FastQCFastQC Report
Fri 27 May 2016
SRR939958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129129
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA18970.16800560431978984No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17220.15250693233456938No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14540.1287718232372032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG201.4205816E-62479.8643108-109
CGTCGCT302.6685757E-62008.6127110-111
CGCCGGT150.00202348152008.6127110-111
CCGCGCA252.774159E-61983.8912108-109
CCGCTCC2450.01923.16108-109
CTCCGCC2150.01821.765110-111
CGACGCG150.00298832241653.2428108-109
CCGCGCT304.793028E-61653.2428108-109
CCGCGCC2600.01430.6908108-109
CATCGCG357.6100077E-61417.0652108-109
CAGCGCG908.367351E-111102.1619108-109
CCGCTCG704.2580723E-81062.799108-109
CGGTGCG300.0080912571004.30634110-111
CGGCGCG502.860137E-6991.9456108-109
CGACGGT250.0082986755991.9456108-109
GGTGTCG552.9512663E-5901.7688108-109
GGTCGCC1400.0885.6658108-109
CGACGCC603.8309714E-5826.6214108-109
CCGCTCT1254.311005E-10793.5565108-109
CCGCGCG951.4433499E-7783.1149108-109
Produced by FastQC (version 0.11.5)