##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939958_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1129129 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76974818643397 34.0 31.0 34.0 30.0 34.0 2 31.945898121472393 34.0 31.0 34.0 30.0 34.0 3 32.066147446394524 34.0 31.0 34.0 30.0 34.0 4 35.31959678654963 37.0 35.0 37.0 33.0 37.0 5 35.337041206097794 37.0 35.0 37.0 33.0 37.0 6 35.428737549031155 37.0 37.0 37.0 33.0 37.0 7 35.37517325301184 37.0 37.0 37.0 33.0 37.0 8 35.366262845077934 37.0 37.0 37.0 33.0 37.0 9 37.03274913672397 39.0 39.0 39.0 34.0 39.0 10-11 37.00563841686822 39.0 38.5 39.0 34.0 39.0 12-13 36.94433496969788 39.0 38.0 39.0 34.0 39.0 14-15 38.44995567379812 41.0 39.0 41.0 34.0 41.0 16-17 38.44393554677986 41.0 39.0 41.0 34.0 41.0 18-19 38.458835527207256 41.0 39.0 41.0 34.0 41.0 20-21 38.49601816975739 41.0 39.0 41.0 34.0 41.0 22-23 38.48045484616904 41.0 39.0 41.0 34.0 41.0 24-25 38.49320361092488 41.0 39.0 41.0 34.0 41.0 26-27 38.452137886813645 41.0 39.0 41.0 34.0 41.0 28-29 38.395506182198844 41.0 39.0 41.0 34.0 41.0 30-31 38.313466840369884 41.0 39.0 41.0 34.0 41.0 32-33 38.21920037480217 40.5 39.0 41.0 33.0 41.0 34-35 38.15549596193172 40.0 38.0 41.0 33.0 41.0 36-37 38.33312314181993 41.0 39.0 41.0 34.0 41.0 38-39 38.29295678350304 41.0 39.0 41.0 33.0 41.0 40-41 38.21936023253322 41.0 39.0 41.0 33.0 41.0 42-43 38.16082839073303 41.0 39.0 41.0 33.0 41.0 44-45 38.07758325222362 41.0 38.5 41.0 33.0 41.0 46-47 37.98429054607578 41.0 38.0 41.0 33.0 41.0 48-49 37.894447401492656 40.0 38.0 41.0 33.0 41.0 50-51 37.785571887711676 40.0 38.0 41.0 33.0 41.0 52-53 37.68818000423335 40.0 38.0 41.0 32.5 41.0 54-55 37.557673658191405 40.0 38.0 41.0 32.0 41.0 56-57 37.37492173170648 40.0 37.5 41.0 31.5 41.0 58-59 37.21671881600774 40.0 37.0 41.0 31.0 41.0 60-61 37.01744929055936 40.0 37.0 41.0 31.0 41.0 62-63 36.781755229030516 40.0 36.0 41.0 31.0 41.0 64-65 36.51255746686162 39.5 36.0 41.0 31.0 41.0 66-67 36.22489370125114 39.0 35.0 41.0 30.0 41.0 68-69 35.850695536116774 39.0 35.0 41.0 30.0 41.0 70-71 35.448348240103655 38.0 35.0 40.0 29.0 41.0 72-73 35.056091465191315 37.0 35.0 40.0 29.0 41.0 74-75 34.63784563145575 37.0 35.0 39.0 29.0 41.0 76-77 34.17275926842726 36.0 34.0 39.0 28.0 40.5 78-79 33.69936118902269 36.0 34.0 38.5 28.0 40.0 80-81 33.242274354834564 35.0 34.0 37.0 26.5 39.0 82-83 32.99782752900687 35.0 34.0 37.0 27.5 39.0 84-85 32.543396724377814 35.0 34.0 36.5 26.0 38.5 86-87 31.860093930808613 35.0 34.0 36.0 13.5 37.0 88-89 16.30428719836263 18.0 16.5 18.5 2.0 19.5 90-91 2.7648054385282816 2.0 2.0 2.0 2.0 2.0 92-93 2.29727382787972 2.0 2.0 2.0 2.0 2.0 94-95 2.10332433229507 2.0 2.0 2.0 2.0 2.0 96-97 2.0000128417567877 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12198593358025178 1101 2 0.12251445370988989 1101 3 0.1708653811340355 1101 4 0.19504184727558993 1101 5 0.24806040283014852 1101 6 0.20489443282625075 1101 7 0.26066564161516226 1101 8 0.17001694080253316 1101 9 0.22366131592933414 1101 10-11 0.22364826373151914 1101 12-13 0.1782636204098651 1101 14-15 0.3013196383534833 1101 16-17 0.3192137271141746 1101 18-19 0.28058540909898966 1101 20-21 0.25186867201508534 1101 22-23 0.2494694301594791 1101 24-25 0.24583445147219152 1101 26-27 0.3297667779557045 1101 28-29 0.33119063878611854 1101 30-31 0.21637100979037882 1101 32-33 0.24172076995269975 1101 34-35 0.36975072046066515 1101 36-37 0.34611543171277503 1101 38-39 0.30365752503826116 1101 40-41 0.36541222142096785 1101 42-43 0.30516186657971645 1101 44-45 0.44498427979741706 1101 46-47 0.4358308890823821 1101 48-49 0.49863924223602396 1101 50-51 0.40461277083132785 1101 52-53 0.2796288286196571 1101 54-55 0.4243083597782018 1101 56-57 0.43482598587883814 1101 58-59 0.30272125529415206 1101 60-61 0.35586474195945783 1101 62-63 0.29352677014683337 1101 64-65 0.4589031455710426 1101 66-67 0.337450161964334 1101 68-69 0.347906556795742 1101 70-71 0.30066979067336064 1101 72-73 0.37290947018595944 1101 74-75 0.32438223225756957 1101 76-77 0.24624747932511326 1101 78-79 0.24391146100008143 1101 80-81 0.30293954356703523 1101 82-83 0.23703014388734545 1101 84-85 0.1995811312096123 1101 86-87 0.2745889585095256 1101 88-89 0.6670502977168269 1101 90-91 -0.14523789877949644 1101 92-93 -0.14338907638977272 1101 94-95 0.015220216670627451 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.11185821042049682 1102 2 0.17413224086958223 1102 3 0.2578127936628434 1102 4 0.2960059970330704 1102 5 0.27547996807332 1102 6 0.27008898235603596 1102 7 0.30321986762704967 1102 8 0.19867657894000956 1102 9 0.2779335785385868 1102 10-11 0.34016728384860784 1102 12-13 0.3660929591826019 1102 14-15 0.3980354414755993 1102 16-17 0.41310922419089025 1102 18-19 0.2876659209403627 1102 20-21 0.3790814925583206 1102 22-23 0.36817883724098266 1102 24-25 0.3387596715584493 1102 26-27 0.3264930566696975 1102 28-29 0.33134500552670687 1102 30-31 0.4280313318061246 1102 32-33 0.42598405913042825 1102 34-35 0.3820411592100754 1102 36-37 0.40787774250975417 1102 38-39 0.45686977634178305 1102 40-41 0.47459299060611926 1102 42-43 0.36458337672522845 1102 44-45 0.31545299335520127 1102 46-47 0.4556044581758272 1102 48-49 0.36525451920672225 1102 50-51 0.42141245774949 1102 52-53 0.3752235457589137 1102 54-55 0.39758334604135115 1102 56-57 0.36522437461422186 1102 58-59 0.29048141563000485 1102 60-61 0.21711434171278654 1102 62-63 0.2350873961524016 1102 64-65 0.272415077863144 1102 66-67 0.20825053671380545 1102 68-69 0.19237722316198358 1102 70-71 0.2630900872314328 1102 72-73 0.1584074956357142 1102 74-75 0.17719048148969563 1102 76-77 0.09022816470553607 1102 78-79 0.16652411314322535 1102 80-81 0.0724749411849217 1102 82-83 0.13184215642643693 1102 84-85 0.01798812969559549 1102 86-87 0.19985984150309832 1102 88-89 1.0708139999886477 1102 90-91 0.13063720844708104 1102 92-93 -0.10427519287044795 1102 94-95 0.03542980756911662 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.0735065577417835 1103 2 0.1036158617308729 1103 3 0.11007846715756386 1103 4 -0.028831499892547185 1103 5 0.06870461466573374 1103 6 -0.013098537407849165 1103 7 -0.04549474769450512 1103 8 0.09653680872813908 1103 9 0.07601516386587548 1103 10-11 0.05151614720548281 1103 12-13 0.09789554283509005 1103 14-15 -0.06495133575171508 1103 16-17 0.059203294439065246 1103 18-19 -8.515362951300176E-5 1103 20-21 0.13990858928716676 1103 22-23 0.1579367515798964 1103 24-25 0.05308336597067864 1103 26-27 0.1386459442468535 1103 28-29 0.001017539871952522 1103 30-31 0.047346652186050164 1103 32-33 0.11132188984614544 1103 34-35 0.04272654154614486 1103 36-37 0.08662351157384762 1103 38-39 0.08196317900976169 1103 40-41 0.08518350628254012 1103 42-43 0.05742504309909435 1103 44-45 0.075086644081928 1103 46-47 0.1398659622223022 1103 48-49 0.048691503149662196 1103 50-51 0.011546325146980507 1103 52-53 0.10463689502046947 1103 54-55 0.06481452789252984 1103 56-57 0.07748969675094486 1103 58-59 0.08467463910540118 1103 60-61 0.01679753391731964 1103 62-63 -7.798139479788802E-4 1103 64-65 -0.08341805051135509 1103 66-67 0.023389381682946464 1103 68-69 0.06909389978378755 1103 70-71 0.1948563638907217 1103 72-73 0.1088888560938841 1103 74-75 0.07683268192771919 1103 76-77 0.1676715667879236 1103 78-79 0.04988145593979709 1103 80-81 0.03309122311458168 1103 82-83 0.07500531536907573 1103 84-85 0.13077756084699388 1103 86-87 0.25558580568800693 1103 88-89 0.7482509605077183 1103 90-91 -0.5420897980998896 1103 92-93 -0.33446387211573114 1103 94-95 -0.11802931192773869 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.08648899135101118 1104 2 0.04176342031461289 1104 3 0.1223016018903067 1104 4 0.15811148847801348 1104 5 0.12826564077818148 1104 6 0.12087828482496121 1104 7 0.10277356644794367 1104 8 0.17892974927639216 1104 9 0.22900938125292214 1104 10-11 0.20492775366334826 1104 12-13 0.26403723431415216 1104 14-15 0.25580005470026634 1104 16-17 0.25435099158921304 1104 18-19 0.2817972316504438 1104 20-21 0.11778527869901012 1104 22-23 -0.007562239589020692 1104 24-25 0.1952789789355407 1104 26-27 0.25155954527247104 1104 28-29 0.2025049320182788 1104 30-31 0.15226057765985956 1104 32-33 0.16780967065435703 1104 34-35 0.21422050151509353 1104 36-37 0.17065953433478853 1104 38-39 0.22430862394876527 1104 40-41 0.347541546119821 1104 42-43 0.22967942850072376 1104 44-45 0.1423189546148862 1104 46-47 0.11079855923850346 1104 48-49 0.12057874388683132 1104 50-51 0.18282623943859022 1104 52-53 0.14216529831858082 1104 54-55 0.2071588462753411 1104 56-57 0.3382300018899542 1104 58-59 0.2701159280659411 1104 60-61 0.29023525394782723 1104 62-63 0.27807213516322804 1104 64-65 0.15520908304525705 1104 66-67 0.31355364484197423 1104 68-69 0.3143806702964014 1104 70-71 0.259294815056208 1104 72-73 0.31247671226871887 1104 74-75 0.3828594196124939 1104 76-77 0.23165826891857222 1104 78-79 0.38416841909352684 1104 80-81 0.4013669499967989 1104 82-83 0.3982109084952796 1104 84-85 0.45937428865186547 1104 86-87 0.4033223853056782 1104 88-89 0.6851835282334626 1104 90-91 -0.621504124064419 1104 92-93 -0.33446387211573114 1104 94-95 -0.11802931192773869 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.16470281731833936 1105 2 -0.022239724596680333 1105 3 0.10352939942816874 1105 4 0.12976602277250038 1105 5 0.05107346886618558 1105 6 0.06504910921467655 1105 7 0.1481673545849702 1105 8 -0.0029489888979554735 1105 9 0.09519738475741946 1105 10-11 0.07290079643870939 1105 12-13 0.15294018491590577 1105 14-15 0.06673713215807453 1105 16-17 0.14443549450091808 1105 18-19 0.11727316475255378 1105 20-21 0.0900613529831702 1105 22-23 -0.029852019437868194 1105 24-25 0.09217665805290665 1105 26-27 0.06908837301194382 1105 28-29 0.09396920212695647 1105 30-31 0.057701392094614334 1105 32-33 -0.04841937585835154 1105 34-35 0.04932947917576058 1105 36-37 -0.04231683535586228 1105 38-39 0.05975895122438146 1105 40-41 0.06366522189332358 1105 42-43 0.04907766578826056 1105 44-45 -0.039368937255581216 1105 46-47 0.015648965947455906 1105 48-49 -0.02287891218160354 1105 50-51 -0.10469698080910206 1105 52-53 -0.1322320805290147 1105 54-55 -0.19254221030580254 1105 56-57 -0.22687032505407956 1105 58-59 -0.3385524363222885 1105 60-61 -0.22436061979296795 1105 62-63 -0.2781243643176552 1105 64-65 -0.2129498322403549 1105 66-67 -0.2317961160106563 1105 68-69 -0.11928365979254352 1105 70-71 -0.3267125771064201 1105 72-73 -0.20415196935175572 1105 74-75 -0.22782296276834302 1105 76-77 -0.11239672134384904 1105 78-79 -0.21538206049071107 1105 80-81 -0.2418993071240152 1105 82-83 -0.18459354478164158 1105 84-85 -0.24416034700485767 1105 86-87 -0.23406871624592895 1105 88-89 0.4449439534891333 1105 90-91 -0.7866583890371959 1105 92-93 -0.33446387211573114 1105 94-95 -0.11802931192773869 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.024638384421525217 1106 2 -0.039707958601969295 1106 3 -0.042976160972266086 1106 4 -0.1314473913782308 1106 5 0.0012475241746940924 1106 6 -0.06627363905738548 1106 7 -0.1538081551484325 1106 8 -0.08815681021558675 1106 9 0.021888027572863678 1106 10-11 -0.001652894038336683 1106 12-13 -0.04760396315002424 1106 14-15 0.0542577629374037 1106 16-17 -0.044744968060975054 1106 18-19 -0.011747945309494412 1106 20-21 -0.07065800355273666 1106 22-23 -0.13399078800556197 1106 24-25 -0.09609388804112484 1106 26-27 -0.2860222944523301 1106 28-29 -0.07934123614646893 1106 30-31 -0.007268805888799079 1106 32-33 -0.06974925931697129 1106 34-35 -0.15459126980260862 1106 36-37 -0.039094830152222926 1106 38-39 -0.002046899628595611 1106 40-41 0.01621406909222145 1106 42-43 -0.1566960695179418 1106 44-45 0.049433864926783144 1106 46-47 -0.12396645470422385 1106 48-49 -0.06107125951383097 1106 50-51 -0.011327298865445812 1106 52-53 -0.062125268948385326 1106 54-55 -0.041499680269545536 1106 56-57 -0.17287245840278587 1106 58-59 -0.031791666875299995 1106 60-61 -0.0020047829941418627 1106 62-63 -0.020708815376764278 1106 64-65 -0.007909435552811317 1106 66-67 0.04562354106794686 1106 68-69 0.03007957886143231 1106 70-71 0.12162903256283641 1106 72-73 0.07187740743654558 1106 74-75 -0.08126659119088941 1106 76-77 0.08944293155455796 1106 78-79 0.111688044519056 1106 80-81 0.07508928378474877 1106 82-83 0.1306047629539151 1106 84-85 0.14665472193257756 1106 86-87 0.10157730642811202 1106 88-89 1.0984441588960188 1106 90-91 -0.34523827069400026 1106 92-93 -0.33446387211573114 1106 94-95 -0.11802931192773869 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.09183565086039636 1107 2 -0.1064572710446754 1107 3 0.06649823967222801 1107 4 -0.03983420139017824 1107 5 0.010693450224088963 1107 6 -0.002561385258452731 1107 7 -0.1088870506119477 1107 8 2.5333507429792235E-4 1107 9 -0.028156420575591312 1107 10-11 -0.04599513089140572 1107 12-13 -0.06310803293361289 1107 14-15 0.014096430048546438 1107 16-17 -0.009544465241297928 1107 18-19 -0.08071480365880745 1107 20-21 -0.018276555098694303 1107 22-23 0.023519212827835645 1107 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114-115 0.0 2215 116-117 0.0 2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.09590530056099666 2216 2 -0.07793404525176584 2216 3 -0.05614647111609372 2216 4 -0.07218378823063887 2216 5 0.007136464994282221 2216 6 0.068163760449373 2216 7 0.040082149586766036 2216 8 0.010531596795644305 2216 9 -0.08215227848509699 2216 10-11 -0.04854851628073931 2216 12-13 -0.04837807344493683 2216 14-15 -0.16185451906391535 2216 16-17 -0.15296740976387468 2216 18-19 -0.13490482206032084 2216 20-21 -0.16902468521071512 2216 22-23 -0.1922285149696492 2216 24-25 -0.21092867494400025 2216 26-27 -0.2263179172826284 2216 28-29 -0.20983121695171292 2216 30-31 -0.3165862013978682 2216 32-33 -0.37153396053420096 2216 34-35 -0.30692021450229845 2216 36-37 -0.35521357899480677 2216 38-39 -0.40168593230671945 2216 40-41 -0.33633269057937554 2216 42-43 -0.4167183507255956 2216 44-45 -0.4547163774327956 2216 46-47 -0.3444320966705021 2216 48-49 -0.36550310341603875 2216 50-51 -0.3689858026147448 2216 52-53 -0.4189023716271336 2216 54-55 -0.45152387213484957 2216 56-57 -0.4656761868307271 2216 58-59 -0.5128835048397704 2216 60-61 -0.4579735305139607 2216 62-63 -0.6105213357693557 2216 64-65 -0.5290433667750136 2216 66-67 -0.5337050538344243 2216 68-69 -0.545251773266898 2216 70-71 -0.637119868809016 2216 72-73 -0.544857601984468 2216 74-75 -0.5505701123859623 2216 76-77 -0.449559910916129 2216 78-79 -0.45238130353515515 2216 80-81 -0.4761767473677878 2216 82-83 -0.4720926447245759 2216 84-85 -0.4212474053642552 2216 86-87 -0.6418328353426901 2216 88-89 -1.340087779951178 2216 90-91 -0.7866583890371959 2216 92-93 -0.33446387211573114 2216 94-95 -0.11802931192773869 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13583.0 3 2359.0 4 1514.0 5 1244.0 6 1167.0 7 1409.0 8 1476.0 9 1878.0 10 2125.0 11 2566.0 12 3115.0 13 3643.0 14 4238.0 15 4752.0 16 5534.0 17 6483.0 18 8130.0 19 10127.0 20 11144.0 21 15403.0 22 18465.0 23 23143.0 24 35109.0 25 57666.0 26 105501.0 27 300198.0 28 469367.0 29 17167.0 30 623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4525966475044 20.713488007127616 15.852041706483494 22.981873638884483 2 12.937928762348088 20.97682978455827 44.06901999961031 22.016221453483325 3 18.52120599987596 26.634416890377338 29.469562058669784 25.37481505107691 4 11.530947396027612 18.443331404760237 40.40540791979026 29.62031327942189 5 12.827766958290729 38.58882779176731 34.95173760568197 13.631667644259993 6 28.364960956631176 37.929058592950845 20.70365742089699 13.002323029520985 7 27.06316107371257 32.17170048772107 23.200537759635967 17.564600678930397 8 23.93172082197871 37.98379104601865 21.57379714806723 16.510690983935405 9 25.24804517464346 18.513827915145214 22.398149370001125 33.8399775402102 10-11 23.523816566802715 27.471272969778664 29.967377235093362 19.037533228325255 12-13 25.35758188735927 24.88672514329845 29.540780719716025 20.214912249626256 14-15 20.72957152352456 28.8054016881161 26.729110105714277 23.735916682645065 16-17 20.248333886859292 30.360943208236467 29.391165725672536 19.999557179231704 18-19 20.95087567485221 28.974570067495186 30.183026606149365 19.891527651503242 20-21 22.04084994526653 28.665592662526524 28.62126563623675 20.6722917559702 22-23 21.010421362420214 29.895945791556812 28.754130418032403 20.339502427990574 24-25 21.443962558751036 28.81800927971915 29.729419756290028 20.00860840523979 26-27 20.060551097350256 29.811739845491523 29.47643714757127 20.651271909586946 28-29 20.228479118887876 29.856476534570998 28.333474298756066 21.581570047785064 30-31 20.887205979121962 28.928049850814215 29.035212097112023 21.149532072951807 32-33 20.28990833975798 29.61295810747439 29.557782175517033 20.5393513772506 34-35 20.661316220407446 29.821401844079542 29.430126106275022 20.087155829237986 36-37 20.17872206637426 29.38078294966689 29.298241341212574 21.14225364274628 38-39 20.920683110610035 29.73256377260703 28.784399302471197 20.56235381431174 40-41 22.065326459598506 28.364960956631176 29.562078380769602 20.00763420300072 42-43 20.284839021936378 29.807798754615284 29.5481295759829 20.359232647465436 44-45 20.076377523186036 29.905024053960222 28.968460617396786 21.050137805456952 46-47 21.21405083033028 28.868534950390966 28.749460867624517 21.16795335165424 48-49 20.79419728950008 28.948457990793774 28.90329037243358 21.354054347272562 50-51 20.15565115542753 29.95715718552599 29.031885238823783 20.855306420222693 52-53 20.298433571363415 29.54396707550687 29.29071877526837 20.866880577861345 54-55 20.32425567416432 28.960091541206385 29.566764590486834 21.14888819414246 56-57 20.846555176600727 29.156234584356618 28.27520150487677 21.722008734165893 58-59 21.024125675631396 29.39788102156618 28.03103099822961 21.546962304572816 60-61 20.54069995545239 28.476152857645143 29.81740793124612 21.16573925565635 62-63 20.772072987231752 28.3941870238033 29.693241427684526 21.14049856128042 64-65 20.882334967926607 29.174080198099595 28.697075356314468 21.246509477659327 66-67 20.98976290574416 29.99519983987658 27.994719823864234 21.020317430515025 68-69 21.472439093405388 28.534225350303977 29.468664494190865 20.524671062099774 70-71 22.051643346331552 28.313195392200534 28.892447187168163 20.742714074299748 72-73 21.380329439771717 29.07657141035258 28.67139184273896 20.871707307136738 74-75 20.67447563564482 29.833925087390366 29.28265946583606 20.208939811128754 76-77 21.00610293420858 28.6690449009812 29.582093808590514 20.742758356219706 78-79 20.73544428571808 28.717451624130856 29.28492543354291 21.262178656608157 80-81 21.231099369514023 29.149857987882694 28.93460357496796 20.684439067635317 82-83 20.836067766107913 29.161585271348724 29.68176959204477 20.32057737049859 84-85 20.85029332369758 28.924842887608843 29.70433821497651 20.520525573717062 86-87 20.03595720561145 30.130234164659203 28.572118109678335 21.261690520051012 88-89 29.681795872257698 18.458416848778324 35.03448011121378 16.825307167750196 90-91 30.486759451360072 15.013038842784693 16.85204395266636 37.64815775318887 92-93 22.31931412700829 17.606128003386292 35.69175816219307 24.382799707412346 94-95 15.074750098197178 6.834042757857778 27.191560653792816 50.89964649015223 96-97 25.797307118080315 7.048842631169326 14.722370643223211 52.43147960752715 98-99 57.142039638081556 14.267547512923388 18.743204491552905 9.847208357442156 100-101 30.41112565493017 6.952434378655051 12.556510070066986 50.079929896347785 102-103 22.613649674476953 1.9640016474526831 37.12823680998749 38.29411186808287 104-105 40.82622338175151 4.595261599210267 41.05714990833451 13.521365110703709 106-107 5.026585923338893 1.553158571818197 5.9876857575238285 87.43256974731908 108-109 17.765522592381945 10.74057998686245 21.371916740145444 50.12198068061016 110-111 39.85105557967931 2.6561948592681452 24.82511084421686 32.66763871683568 112-113 26.8164490736119 2.6708299594393234 38.65401040453451 31.858710562414267 114-115 27.607306486616835 3.8086434638158777 23.36226991399405 45.22178013557323 116-117 18.848636258001818 3.9957587729491055 19.702823611433885 57.45278135761519 118-119 19.404185779454725 4.086876691757787 15.105149253260478 61.403788275527006 120-121 24.642654238692256 4.140118017469483 29.493502701454734 41.72372504238353 122-123 33.71335026647678 1.564174656076111 33.47311741128452 31.249357666162584 124-125 37.97872415972503 1.8649204286414283 19.458854399681858 40.69750101195168 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 7.5 2 13.5 3 24.0 4 34.5 5 35.0 6 34.0 7 40.5 8 44.0 9 46.0 10 51.0 11 56.5 12 64.0 13 81.5 14 107.0 15 142.0 16 191.5 17 263.0 18 373.5 19 587.0 20 900.5 21 1318.5 22 1982.0 23 2882.0 24 4093.5 25 5682.5 26 7701.5 27 10420.0 28 13747.5 29 17682.0 30 22256.5 31 27511.5 32 33587.5 33 40365.0 34 46731.5 35 51366.5 36 53729.5 37 54069.0 38 53099.0 39 51689.0 40 49975.5 41 48028.5 42 46315.5 43 45037.5 44 43545.0 45 41760.5 46 39713.5 47 37278.0 48 35362.5 49 33273.5 50 30768.0 51 28097.0 52 25092.0 53 21804.5 54 18742.0 55 16042.5 56 13513.0 57 11210.5 58 9158.5 59 7481.0 60 5986.5 61 4568.0 62 3452.5 63 2634.0 64 1960.0 65 1468.0 66 1063.5 67 785.0 68 587.5 69 399.5 70 276.5 71 207.5 72 153.5 73 105.5 74 70.0 75 51.5 76 39.0 77 25.5 78 13.5 79 8.0 80 5.5 81 6.5 82 5.5 83 3.0 84 4.0 85 6.0 86 5.5 87 3.5 88 3.5 89 2.0 90 0.0 91 1.0 92 1.0 93 1.5 94 2.0 95 1.5 96 2.0 97 4.0 98 3.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.199468794088187E-4 3 0.03887952572292448 4 0.043219153878786214 5 0.04490186683718158 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.413564791976824E-4 12-13 0.001505585278564274 14-15 4.428191995777276E-5 16-17 3.542553596621821E-4 18-19 0.007572208312779142 20-21 0.001859840638226456 22-23 5.313830394932731E-4 24-25 0.0 26-27 0.0 28-29 9.29920319113228E-4 30-31 0.0 32-33 0.0011956118388598645 34-35 1.7712767983109104E-4 36-37 1.3284575987331827E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 8.856383991554552E-5 46-47 0.0 48-49 1.3284575987331827E-4 50-51 1.3284575987331827E-4 52-53 0.0 54-55 0.0017712767983109107 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2140959978886383E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.7712767983109104E-4 80-81 0.0 82-83 8.856383991554553E-4 84-85 8.856383991554552E-5 86-87 7.970745592399096E-4 88-89 19.251254728202003 90-91 26.235531989701798 92-93 40.36974517526341 94-95 44.758747671878055 96-97 48.45115128563698 98-99 93.03564960248121 100-101 51.69037373054805 102-103 53.12298240502192 104-105 74.87975244635466 106-107 81.65324776885546 108-109 12.565659016817389 110-111 39.01679967479358 112-113 52.09422484056295 114-115 86.77812721132838 116-117 66.46406212221987 118-119 71.92721115125022 120-121 80.43629204457595 122-123 75.87131319804912 124-125 50.11482301845051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1129129.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.00229307597336 #Duplication Level Percentage of deduplicated Percentage of total 1 86.89856722196677 66.91388941108058 2 9.405287198923572 14.484573627104268 3 1.9551724018285224 4.51658274898964 4 0.6800389358951437 2.0945822977948367 5 0.30717845779529096 1.1826722816889255 6 0.1810793520375696 0.8366115201362573 7 0.10263980572878367 0.553245028139214 8 0.08089960979312555 0.49835643704177107 9 0.05327658988340356 0.369217762946125 >10 0.2983005368546422 4.056722420679463 >50 0.0206403760490682 1.0843641200060001 >100 0.0157685303908226 2.462366012478827 >500 6.905897120443023E-4 0.39785252400661736 >1k 4.6039314136286816E-4 0.5489638079075492 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1897 0.16800560431978984 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1722 0.15250693233456938 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1454 0.1287718232372032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.856383991554552E-5 0.0 9 0.0 0.0 0.0 8.856383991554552E-5 0.0 10-11 0.0 0.0 0.0 8.856383991554552E-5 0.0 12-13 0.0 0.0 0.0 8.856383991554552E-5 0.0 14-15 0.0 0.0 0.0 8.856383991554552E-5 0.0 16-17 0.0 0.0 0.0 8.856383991554552E-5 0.0 18-19 0.0 0.0 0.0 1.7712767983109104E-4 0.0 20-21 0.0 0.0 0.0 1.7712767983109104E-4 0.0 22-23 0.0 0.0 0.0 1.7712767983109104E-4 0.0 24-25 0.0 0.0 0.0 1.7712767983109104E-4 0.0 26-27 0.0 0.0 0.0 3.985372796199548E-4 0.0 28-29 0.0 0.0 0.0 6.642287993665914E-4 0.0 30-31 0.0 0.0 0.0 0.0014170214386487283 0.0 32-33 0.0 0.0 0.0 0.004605319675608367 0.0 34-35 0.0 0.0 0.0 0.008634974391765688 0.0 36-37 0.0 0.0 0.0 0.01363883134699401 0.0 38-39 0.0 0.0 0.0 0.024576465576563882 0.0 40-41 0.0 0.0 0.0 0.038082451163684576 0.0 42-43 0.0 0.0 0.0 0.05309402202936954 0.0 44-45 0.0 0.0 0.0 0.06916835897404106 0.0 46-47 0.0 0.0 0.0 0.08750107383655897 0.0 48-49 0.0 0.0 0.0 0.11141331061375627 0.0 50-51 0.0 0.0 0.0 0.1425877822640283 0.0 52-53 0.0 0.0 0.0 0.17190241327607386 0.0 54-55 0.0 0.0 0.0 0.20480387980469902 0.0 56-57 0.0 0.0 0.0 0.24102649033015716 0.0 58-59 0.0 0.0 0.0 0.2826957770104213 0.0 60-61 0.0 0.0 0.0 0.33060881440473144 0.0 62-63 0.0 0.0 0.0 0.38264007035511444 0.0 64-65 0.0 0.0 0.0 0.43334286870676425 0.0 66-67 0.0 0.0 0.0 0.4952047108877728 0.0 68-69 0.0 0.0 0.0 0.5727866346537906 0.0 70-71 0.0 0.0 0.0 0.6598448892907719 0.0 72-73 0.0 0.0 0.0 0.7460175055285977 0.0 74-75 0.0 0.0 0.0 0.8290903873693795 0.0 76-77 0.0 0.0 0.0 0.9233665949594776 0.0 78-79 0.0 0.0 0.0 0.9515741779725788 0.0 80-81 0.0 0.0 0.0 0.9521498429320299 0.0 82-83 0.0 0.0 0.0 0.9521498429320299 0.0 84-85 0.0 0.0 0.0 0.9521498429320299 0.0 86-87 0.0 0.0 0.0 0.9521498429320299 0.0 88-89 0.0 0.0 0.0 0.9521498429320299 0.0 90-91 0.0 0.0 0.0 0.9521498429320299 0.0 92-93 0.0 0.0 0.0 0.9521498429320299 0.0 94-95 0.0 0.0 0.0 0.9521498429320299 0.0 96-97 0.0 0.0 0.0 0.9521498429320299 0.0 98-99 0.0 0.0 0.0 0.9521498429320299 0.0 100-101 0.0 0.0 0.0 0.9521498429320299 0.0 102-103 0.0 0.0 0.0 0.9521498429320299 0.0 104-105 0.0 0.0 0.0 0.9521498429320299 0.0 106-107 0.0 0.0 0.0 0.9521498429320299 0.0 108-109 0.0 0.0 0.0 0.9521498429320299 0.0 110-111 0.0 0.0 0.0 0.9521498429320299 0.0 112-113 0.0 0.0 0.0 0.9521498429320299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTCG 20 1.4205816E-6 2479.8643 108-109 CGTCGCT 30 2.6685757E-6 2008.6127 110-111 CGCCGGT 15 0.0020234815 2008.6127 110-111 CCGCGCA 25 2.774159E-6 1983.8912 108-109 CCGCTCC 245 0.0 1923.16 108-109 CTCCGCC 215 0.0 1821.765 110-111 CGACGCG 15 0.0029883224 1653.2428 108-109 CCGCGCT 30 4.793028E-6 1653.2428 108-109 CCGCGCC 260 0.0 1430.6908 108-109 CATCGCG 35 7.6100077E-6 1417.0652 108-109 CAGCGCG 90 8.367351E-11 1102.1619 108-109 CCGCTCG 70 4.2580723E-8 1062.799 108-109 CGGTGCG 30 0.008091257 1004.30634 110-111 CGGCGCG 50 2.860137E-6 991.9456 108-109 CGACGGT 25 0.0082986755 991.9456 108-109 GGTGTCG 55 2.9512663E-5 901.7688 108-109 GGTCGCC 140 0.0 885.6658 108-109 CGACGCC 60 3.8309714E-5 826.6214 108-109 CCGCTCT 125 4.311005E-10 793.5565 108-109 CCGCGCG 95 1.4433499E-7 783.1149 108-109 >>END_MODULE