FastQCFastQC Report
Fri 27 May 2016
SRR939958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129129
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.2091877898805185No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19890.17615347759202005No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA17800.15764363504967102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.1402851224262241No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14600.12930320627669645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG15150.032.6244741
TACCTGG14900.031.5718172
GTATCAA73250.029.9983081
TAGGACC13300.028.6541214
GTACATG54450.026.7945351
TACATGG52850.026.5904542
GTACCGT900.0095334926.4418286
CGCGCAG450.0095354726.44182864-65
TATCAAC82950.025.986692
GAGTACT34950.025.87440512-13
CAACGCA83050.025.8119985
TCAACGC83050.025.8119984
ATCAACG83600.025.6421813
ACATGGG52700.025.3102343
AACGCAG84650.025.3016876
AGTACTT37250.025.23508512-13
AGAGTAC66950.023.90428210-11
ACCTGGG19200.023.8807663
GTACTTT38750.023.64725314-15
GTCCTAC42750.023.123311
Produced by FastQC (version 0.11.5)