##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939958_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1129129 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8214145593639 34.0 31.0 34.0 31.0 34.0 2 32.911144785051135 34.0 31.0 34.0 31.0 34.0 3 32.95715901371765 34.0 33.0 34.0 31.0 34.0 4 36.36384239533304 37.0 37.0 37.0 35.0 37.0 5 36.26523187341748 37.0 37.0 37.0 35.0 37.0 6 36.39430127115679 37.0 37.0 37.0 35.0 37.0 7 36.393703465237365 37.0 37.0 37.0 35.0 37.0 8 36.416062292262446 37.0 37.0 37.0 35.0 37.0 9 38.25547036698198 39.0 39.0 39.0 37.0 39.0 10-11 38.251080257437366 39.0 39.0 39.0 37.0 39.0 12-13 38.23699329306041 39.0 39.0 39.0 37.0 39.0 14-15 39.77694266996951 41.0 40.0 41.0 37.0 41.0 16-17 39.803045090507815 41.0 40.0 41.0 38.0 41.0 18-19 39.83581149718057 41.0 40.0 41.0 38.0 41.0 20-21 39.85009241636695 41.0 40.0 41.0 38.0 41.0 22-23 39.80171796136668 41.0 40.0 41.0 37.5 41.0 24-25 39.77511072694085 41.0 40.0 41.0 37.0 41.0 26-27 39.68943052565296 41.0 40.0 41.0 37.0 41.0 28-29 39.61236625753125 41.0 40.0 41.0 37.0 41.0 30-31 39.49961917548836 41.0 40.0 41.0 36.5 41.0 32-33 39.37371017837643 41.0 39.0 41.0 36.0 41.0 34-35 39.29352447771689 41.0 39.0 41.0 35.0 41.0 36-37 39.28883103702057 41.0 39.5 41.0 35.5 41.0 38-39 39.34896986969602 41.0 40.0 41.0 36.0 41.0 40-41 39.2869069875984 41.0 40.0 41.0 35.0 41.0 42-43 39.20359454057065 41.0 40.0 41.0 35.0 41.0 44-45 39.072714455124256 41.0 40.0 41.0 35.0 41.0 46-47 38.88403450801458 41.0 39.0 41.0 35.0 41.0 48-49 38.6882309284413 41.0 39.0 41.0 35.0 41.0 50-51 38.54717928598061 41.0 39.0 41.0 35.0 41.0 52-53 38.452198995863185 41.0 39.0 41.0 35.0 41.0 54-55 38.35389401919532 41.0 39.0 41.0 35.0 41.0 56-57 38.22417412005183 41.0 38.5 41.0 34.5 41.0 58-59 38.07537447005612 41.0 38.0 41.0 34.0 41.0 60-61 37.911981713338335 40.0 38.0 41.0 34.0 41.0 62-63 37.718467951846065 40.0 37.0 41.0 34.0 41.0 64-65 37.49445767489809 40.0 37.0 41.0 34.0 41.0 66-67 37.23712215344748 39.0 36.5 41.0 33.5 41.0 68-69 36.92077167444995 39.0 36.0 41.0 33.0 41.0 70-71 36.565429193652804 39.0 35.0 41.0 32.5 41.0 72-73 36.222482107890244 37.5 35.0 40.0 32.0 41.0 74-75 35.86334289527592 37.0 35.0 39.5 32.0 41.0 76-77 35.46188256611955 36.5 35.0 39.0 32.0 41.0 78-79 35.06998668885486 36.0 35.0 39.0 31.5 40.5 80-81 34.760085871499186 36.0 35.0 37.5 31.5 39.0 82-83 34.48824137897441 35.0 35.0 37.0 31.5 39.0 84-85 34.18568560368213 35.0 35.0 37.0 31.0 39.0 86-87 33.93827100357886 35.0 35.0 36.0 31.0 37.0 88-89 33.70423795686764 35.0 35.0 36.0 31.0 37.0 90-91 33.51271865305027 35.0 35.0 36.0 31.0 37.0 92-93 33.36337566389669 35.0 35.0 35.5 31.0 36.0 94-95 33.25290910073163 35.0 34.5 35.0 31.0 36.0 96-97 33.14204090055255 35.0 34.0 35.0 31.0 36.0 98-99 33.043818288255814 35.0 34.0 35.0 31.0 36.0 100-101 33.01148938695224 35.0 34.0 35.0 31.0 36.0 102-103 32.17782733416642 34.5 33.0 35.0 28.0 35.0 104-105 32.846819539662874 35.0 34.0 35.0 30.0 35.0 106-107 32.91025826101358 35.0 34.0 35.0 31.0 35.0 108-109 32.91122847787985 35.0 34.0 35.0 31.0 35.0 110-111 32.892858566204566 35.0 34.0 35.0 31.0 35.0 112-113 32.8259273298268 35.0 34.0 35.0 30.5 35.0 114-115 32.776804953198436 35.0 34.0 35.0 30.0 35.0 116-117 32.7235656864716 35.0 34.0 35.0 30.0 35.0 118-119 32.65118688830063 35.0 34.0 35.0 30.0 35.0 120-121 32.558515900309004 35.0 34.0 35.0 30.0 35.0 122-123 32.406086018515154 35.0 34.0 35.0 30.0 35.0 124-125 30.40076643147063 33.5 30.5 34.5 23.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10713549984798476 1101 2 0.09455259067069477 1101 3 0.14204951869914595 1101 4 0.04841600643927535 1101 5 -0.08401490710917159 1101 6 -0.03037010467482304 1101 7 0.032936247776170546 1101 8 0.04015083615843196 1101 9 0.027883663751467225 1101 10-11 0.09278551435964744 1101 12-13 0.05930727409963765 1101 14-15 0.1285336629245819 1101 16-17 0.09530014390669095 1101 18-19 0.17211585033297183 1101 20-21 0.09952129920920783 1101 22-23 0.15015460778427325 1101 24-25 0.16729791686997686 1101 26-27 0.1645393404815394 1101 28-29 0.1223479764530424 1101 30-31 0.13840181281687336 1101 32-33 0.10780782748130235 1101 34-35 0.15418295526387737 1101 36-37 0.165683643895143 1101 38-39 0.11169975558239287 1101 40-41 0.1695196205628804 1101 42-43 0.13969922615495278 1101 44-45 0.1805186290898817 1101 46-47 0.11117828521076234 1101 48-49 0.13129167123867092 1101 50-51 0.05142699007372897 1101 52-53 -0.014557386703415887 1101 54-55 0.0052328610985696855 1101 56-57 0.06558561113299533 1101 58-59 0.17195043037810365 1101 60-61 0.13855793182227671 1101 62-63 0.09532297777626297 1101 64-65 0.10360534662022758 1101 66-67 -0.06680159970325406 1101 68-69 -0.058976385803482856 1101 70-71 0.07860000921387922 1101 72-73 0.026982017032210592 1101 74-75 0.02015354441256534 1101 76-77 0.04144181445966666 1101 78-79 0.11894051740819123 1101 80-81 0.1079016130699273 1101 82-83 0.07420519188847408 1101 84-85 0.05050367463185523 1101 86-87 0.12810892461877188 1101 88-89 -0.017390111468557734 1101 90-91 -0.063958691686679 1101 92-93 -0.006587990580754877 1101 94-95 0.019513388913409813 1101 96-97 -0.06315774718692779 1101 98-99 0.08539309626542746 1101 100-101 0.0654444505693732 1101 102-103 0.1260998898888701 1101 104-105 0.06730867604864699 1101 106-107 0.1122153212899164 1101 108-109 0.0010688707985551105 1101 110-111 0.0015393751100134523 1101 112-113 -0.01103319042823614 1101 114-115 -0.011431881322884863 1101 116-117 0.04293999095397538 1101 118-119 0.03087567247776235 1101 120-121 0.11809820191713527 1101 122-123 0.05781979730182485 1101 124-125 0.17324139466812127 1102 1 -0.021207103395894933 1102 2 0.10651018231235554 1102 3 0.1748242855678157 1102 4 0.10716747401039584 1102 5 0.01295547266068553 1102 6 0.07866538979288151 1102 7 0.04704311462546684 1102 8 -0.027484328624602483 1102 9 0.0016492239584522395 1102 10-11 0.13923567228412992 1102 12-13 0.09817903308987042 1102 14-15 0.17030795147377376 1102 16-17 0.2046773798525905 1102 18-19 0.13187000993355014 1102 20-21 0.12496255881018214 1102 22-23 0.16207563368072897 1102 24-25 0.24997306678952924 1102 26-27 0.1889798845798225 1102 28-29 0.21526568573757743 1102 30-31 0.25107174470576155 1102 32-33 0.20560143065006997 1102 34-35 0.1422040171331247 1102 36-37 0.11409056332005463 1102 38-39 0.15601981060889614 1102 40-41 0.1830191007363311 1102 42-43 0.16979283446269022 1102 44-45 0.14703448676414155 1102 46-47 0.19828678467117555 1102 48-49 0.32112441754355103 1102 50-51 0.349611352584553 1102 52-53 0.3951639625528216 1102 54-55 0.3422638807096732 1102 56-57 0.28590193447696777 1102 58-59 0.36111016931845086 1102 60-61 0.3521178937148406 1102 62-63 0.32917692826031697 1102 64-65 0.30713107506916515 1102 66-67 0.2660186272810847 1102 68-69 0.2041536815170204 1102 70-71 0.2626539842068567 1102 72-73 0.20292215198946195 1102 74-75 0.27417142802676153 1102 76-77 0.23264389850948675 1102 78-79 0.2722092973772732 1102 80-81 0.21347680212471687 1102 82-83 0.22990529743501043 1102 84-85 0.2654381132389574 1102 86-87 0.3052019676398601 1102 88-89 0.2915335115840705 1102 90-91 0.29953169646364586 1102 92-93 0.26314229257647526 1102 94-95 0.1615094437659792 1102 96-97 0.17714323830931988 1102 98-99 0.2628326244289525 1102 100-101 0.328075049575574 1102 102-103 0.2929636162743279 1102 104-105 0.25437112243535864 1102 106-107 0.28896701813814474 1102 108-109 0.25385478527819316 1102 110-111 0.25808918951414483 1102 112-113 0.3228237248459749 1102 114-115 0.3497552601845726 1102 116-117 0.35444298143897157 1102 118-119 0.29515054410234853 1102 120-121 0.38394935363928084 1102 122-123 0.38642824339600423 1102 124-125 0.293102128592043 1103 1 -0.03544998546843914 1103 2 0.038530607924016635 1103 3 0.030170197517499275 1103 4 -0.07219024356804482 1103 5 -0.13221979642098347 1103 6 -0.01505584277098393 1103 7 -0.06489017420251741 1103 8 0.0325768076671622 1103 9 0.055354294054374975 1103 10-11 0.02257134800412075 1103 12-13 0.03750594828376563 1103 14-15 -0.007668929169916794 1103 16-17 0.03589846601729363 1103 18-19 -0.06822075754943313 1103 20-21 0.011047025273057898 1103 22-23 -0.0016694912270409645 1103 24-25 0.077869135093529 1103 26-27 0.07129955008700506 1103 28-29 0.14482894774523913 1103 30-31 -0.14086810280486617 1103 32-33 -0.11555442004620176 1103 34-35 -0.03425265456967708 1103 36-37 -0.04278317032716217 1103 38-39 -0.05892578746097854 1103 40-41 -0.10905484841045165 1103 42-43 -0.18741234689659336 1103 44-45 -0.13214696621830768 1103 46-47 -0.0819007233807767 1103 48-49 -0.19687799511469706 1103 50-51 -0.12595521218829475 1103 52-53 -0.11613336829758225 1103 54-55 -0.15528012687965997 1103 56-57 -0.20602151632705556 1103 58-59 -0.15596992859330783 1103 60-61 -0.08937924271747022 1103 62-63 -0.19055885052468824 1103 64-65 -0.2505958729181401 1103 66-67 -0.11098733409296102 1103 68-69 -0.0496878326017125 1103 70-71 -0.07342755427269765 1103 72-73 -0.08872021241563033 1103 74-75 -0.1535079695113808 1103 76-77 -0.21599224685902385 1103 78-79 -0.14794166944281528 1103 80-81 -0.23399224909157113 1103 82-83 -0.19783910133289595 1103 84-85 -0.15925122773055023 1103 86-87 -0.1875700127309443 1103 88-89 -0.17398304323363334 1103 90-91 -0.16234127590790592 1103 92-93 -0.1545848896494988 1103 94-95 -0.09003008986483962 1103 96-97 -0.11503319025374736 1103 98-99 -0.15953365457271929 1103 100-101 -0.03955433800743435 1103 102-103 -0.14315305800509748 1103 104-105 -0.15403570327936222 1103 106-107 -0.12906584554666978 1103 108-109 -0.13461832997894163 1103 110-111 -0.15319935738628487 1103 112-113 -0.031920779329333016 1103 114-115 -0.037348144474435685 1103 116-117 -0.08325187585112559 1103 118-119 -0.06374935400383208 1103 120-121 -0.08275010644058511 1103 122-123 -0.07734381895831888 1103 124-125 -0.11637458222861241 1104 1 0.08355039868722258 1104 2 0.1461077122464971 1104 3 0.1384581588000131 1104 4 0.07520386296587134 1104 5 0.04040128302263213 1104 6 0.07627294152842978 1104 7 0.04342933065350252 1104 8 0.04467264154969541 1104 9 0.03707366665269518 1104 10-11 0.06643550335171255 1104 12-13 0.1037851922744295 1104 14-15 0.009592728151964991 1104 16-17 0.011871275456933006 1104 18-19 0.08738200071076108 1104 20-21 0.16092518822039636 1104 22-23 0.10558429146702508 1104 24-25 0.16934493497659275 1104 26-27 0.08780078197621322 1104 28-29 0.15844732232742587 1104 30-31 0.06701531214746836 1104 32-33 0.1096267020262971 1104 34-35 0.19978714215451987 1104 36-37 0.17297092949247883 1104 38-39 0.13918249937374583 1104 40-41 0.1865923637572564 1104 42-43 0.22310642096636002 1104 44-45 0.21703813935690874 1104 46-47 0.22179178986470305 1104 48-49 0.016825772427189634 1104 50-51 0.16666325364494838 1104 52-53 0.07810407144574327 1104 54-55 0.12833152216203558 1104 56-57 0.2118147973106943 1104 58-59 0.04408166768048716 1104 60-61 0.12631289651135091 1104 62-63 0.17286536209702774 1104 64-65 0.09297841952007957 1104 66-67 0.07238897735926741 1104 68-69 0.17099213418740788 1104 70-71 0.1578251424211956 1104 72-73 0.08616017548019528 1104 74-75 0.07454981380904968 1104 76-77 0.03224553761049975 1104 78-79 0.11153801931223484 1104 80-81 0.07244962455163773 1104 82-83 0.12325757859333919 1104 84-85 0.1323324962361454 1104 86-87 0.11308643156479548 1104 88-89 0.17003229595553648 1104 90-91 0.14603376816860703 1104 92-93 0.1906223459956422 1104 94-95 0.209488532672367 1104 96-97 0.2015904982940171 1104 98-99 0.24329467741437583 1104 100-101 0.27922276271007007 1104 102-103 0.28131698179613096 1104 104-105 0.2695978685459224 1104 106-107 0.2654625836622344 1104 108-109 0.23601415246649537 1104 110-111 0.1732388887459564 1104 112-113 0.1951890069516935 1104 114-115 0.2902448217592948 1104 116-117 0.3176134319634585 1104 118-119 0.2890626992462231 1104 120-121 0.23993332788850807 1104 122-123 0.3861799812311659 1104 124-125 0.4165135360958345 1105 1 -0.0437160853859524 1105 2 -0.007543089123764446 1105 3 -0.05935477203183126 1105 4 -0.050948491333294044 1105 5 -0.14146198112707253 1105 6 -0.005210693216795903 1105 7 -0.05288947257069054 1105 8 0.04230401049244392 1105 9 0.014698963882921134 1105 10-11 0.04945828774771144 1105 12-13 0.04797296440541743 1105 14-15 0.049984906253570216 1105 16-17 0.03324164034179944 1105 18-19 0.003136068352873167 1105 20-21 -0.014688043691755581 1105 22-23 0.034639388573900476 1105 24-25 -0.07394928591731542 1105 26-27 0.0026125920465602803 1105 28-29 0.033312016719129645 1105 30-31 -0.04881181499670362 1105 32-33 -0.03262048004328477 1105 34-35 0.0715837427323649 1105 36-37 0.1654905280815413 1105 38-39 0.04628363980936001 1105 40-41 -0.06201073708990634 1105 42-43 -0.0035959612228921856 1105 44-45 0.012570456857368129 1105 46-47 -0.0469387801025718 1105 48-49 -0.10837787678993749 1105 50-51 -0.11103948739430791 1105 52-53 -0.12923717427219117 1105 54-55 -0.13718210861152613 1105 56-57 -0.045529285311751266 1105 58-59 -0.16237553227264812 1105 60-61 -0.06550863935190421 1105 62-63 -0.08568606287643377 1105 64-65 -0.04371579827903105 1105 66-67 -0.12246067775419078 1105 68-69 -0.09199536206382675 1105 70-71 -0.08823119187078987 1105 72-73 -0.16088981225112065 1105 74-75 -0.18466386257604483 1105 76-77 -0.03351372488275928 1105 78-79 -0.10064862247809003 1105 80-81 -0.22254423373429688 1105 82-83 -0.15370075133203187 1105 84-85 -0.17180669482605992 1105 86-87 -0.21109622676947737 1105 88-89 -0.03908109401272242 1105 90-91 -0.07549071063308332 1105 92-93 -0.18194040301641934 1105 94-95 -0.22528569025527645 1105 96-97 -0.20353954614590464 1105 98-99 -0.28158092248039424 1105 100-101 -0.11739067331183861 1105 102-103 -0.15829692655196226 1105 104-105 -0.10589094483955108 1105 106-107 -0.11390836776185864 1105 108-109 -0.112457198113745 1105 110-111 -0.15170328629767482 1105 112-113 -0.16386952391493992 1105 114-115 -0.1132377917003069 1105 116-117 -0.19535324804034104 1105 118-119 -0.16373415702145167 1105 120-121 -0.3099589802685898 1105 122-123 -0.247209979663765 1105 124-125 -0.18486269418123058 1106 1 0.006785900213948537 1106 2 0.036419750248050775 1106 3 0.0171524460382102 1106 4 0.05370091154528467 1106 5 0.0537913179958025 1106 6 0.05940670943115123 1106 7 0.026094880469685222 1106 8 -0.013428283073636749 1106 9 0.005731692046722969 1106 10-11 0.06306991738107115 1106 12-13 0.04167201809228516 1106 14-15 -0.06536619537599364 1106 16-17 -0.01302572014368053 1106 18-19 -0.021909157198294338 1106 20-21 0.08223806028016867 1106 22-23 0.12530923123943438 1106 24-25 0.09576220749885067 1106 26-27 0.12744700526755537 1106 28-29 0.09827897143591713 1106 30-31 0.04202667753297362 1106 32-33 0.10621547553116528 1106 34-35 0.12347564825961399 1106 36-37 0.1927645985252937 1106 38-39 0.15346315101304242 1106 40-41 0.13434022760172581 1106 42-43 0.1887910109354678 1106 44-45 0.0762220867790262 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-0.12139032395200644 2216 80-81 -0.15381738940392609 2216 82-83 -0.15463575178281275 2216 84-85 -0.09540274195698828 2216 86-87 -0.05887071089307483 2216 88-89 -0.10212562853346441 2216 90-91 -0.11700421632961167 2216 92-93 -0.08414655632078905 2216 94-95 -0.008312187330012932 2216 96-97 -0.07714445770432832 2216 98-99 -0.08344674832736843 2216 100-101 -0.1799004680504268 2216 102-103 -0.03620668244438008 2216 104-105 -0.18513060689006267 2216 106-107 -0.23048390932391527 2216 108-109 -0.0934631200353806 2216 110-111 -0.1302512204625117 2216 112-113 -0.17636503875665 2216 114-115 -0.14002016183643917 2216 116-117 -0.19614423936167213 2216 118-119 -0.268505034260933 2216 120-121 -0.21364833684427254 2216 122-123 -0.27089116160356497 2216 124-125 -0.17556896214833273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101.0 3 4.0 4 13.0 5 45.0 6 67.0 7 150.0 8 323.0 9 640.0 10 1244.0 11 1997.0 12 2401.0 13 4104.0 14 7031.0 15 7531.0 16 2914.0 17 1819.0 18 1667.0 19 1481.0 20 1481.0 21 1570.0 22 1619.0 23 1762.0 24 2193.0 25 2900.0 26 3711.0 27 4764.0 28 6291.0 29 8242.0 30 10390.0 31 13735.0 32 17812.0 33 23667.0 34 34154.0 35 54891.0 36 120280.0 37 398502.0 38 383178.0 39 4455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.49978785766356 20.859747785349157 16.06107927745826 22.57938507952902 2 13.24277385489169 21.38444765832779 43.510174656748696 21.86260383003182 3 18.443773917772017 27.027469846226605 29.73530925164441 24.79344698435697 4 11.699637508203226 18.757644166432712 40.031564152545904 29.511154172818166 5 12.509908513607693 38.398128066762546 34.93258664075232 14.15937677887744 6 28.063312517878824 38.45220519533198 20.21044539640732 13.274036890381879 7 26.54532830172637 32.689798951227004 23.007734280139825 17.757138466906795 8 23.98317641296964 37.46932369994925 21.36593781578544 17.181562071295662 9 25.33705183375859 18.116707656963907 22.53108369371436 34.015156815563145 10-11 23.453476086434765 27.555531741723044 29.61946774903488 19.371524422807315 12-13 25.68608192686575 24.807440071063624 29.630981048223898 19.875496953846728 14-15 20.90536156630465 28.384577847172466 27.49734529889853 23.212715287624356 16-17 19.788615826889576 30.17786275970239 29.879801156466623 20.153720256941412 18-19 20.409669754297337 28.21564232253356 31.783967996570805 19.59071992659829 20-21 21.519318067586557 27.887303485874188 30.438438895902927 20.154939550636332 22-23 20.392179264236557 29.005430303867634 30.67291397376645 19.929476458129358 24-25 20.83353629213314 28.033510785747247 31.579031271006237 19.553921651113377 26-27 19.617067668973164 28.925437217536704 31.37529015728052 20.08220495620961 28-29 19.68238350091088 28.979062622605568 30.307918758618367 21.030635117865188 30-31 20.385491826000397 28.04878804813268 30.95049369912561 20.61522642674132 32-33 19.821074474218623 28.71430102317804 31.394774202061942 20.069850300541393 34-35 20.15872411389664 29.00851009937748 31.256481766033815 19.576284020692057 36-37 19.734149065341516 28.546871083817706 31.143031487102007 20.575948363738775 38-39 20.52976232122282 28.9262785740159 30.504973302430454 20.03898580233082 40-41 21.586151803735447 27.643431352839226 31.152729227572756 19.61768761585257 42-43 19.991648429895964 29.022768877603887 31.022761792496695 19.962820900003454 44-45 19.87934948088305 29.181785252172247 30.251946411791742 20.686918855152957 46-47 21.06951464358811 28.267452168884155 29.873026022713084 20.79000716481465 48-49 20.87153017945691 28.51755645280566 29.621903254632553 20.989010113104882 50-51 20.16386081661174 29.58457359610815 29.56761362076432 20.683951966515785 52-53 20.299363491682527 29.248606669388526 29.795488380867024 20.656541458061923 54-55 20.333062032770393 28.618430666469465 30.089298919786845 20.959208380973298 56-57 20.754758756528265 28.844666995533725 28.864505295674807 21.536068952263204 58-59 20.956728593455665 29.032510899994595 28.68401218992693 21.326748316622812 60-61 20.46550925536409 28.166976492499973 30.449488056723368 20.91802619541257 62-63 20.694269653865945 28.092804276570703 30.312789769813726 20.90013629974963 64-65 20.750994793331852 28.870704764468897 29.346868249774825 21.031432192424425 66-67 20.81325517279248 29.600470805372993 28.703230543188603 20.88304347864593 68-69 21.305271585443293 28.195936868152355 30.10586035785105 20.392931188553302 70-71 21.905114473191283 27.99542833458356 29.559554311332008 20.53990288089315 72-73 21.186197502676844 28.771070444563907 29.30608460149372 20.736647451265533 74-75 20.57541698069928 29.43224379145341 29.870324825595656 20.122014402251647 76-77 20.85262179963494 28.239111740111184 30.152444937646628 20.75582152260725 78-79 20.60610435123002 28.323468797630742 29.928112731140548 21.14231411999869 80-81 20.984891894548806 28.757033075937294 29.609460035124417 20.64861499438948 82-83 20.723097183758455 28.79918946373709 30.233525133089312 20.244188219415143 84-85 20.695066728425186 28.587034785219405 30.20886010367283 20.509038382682583 86-87 19.870315969211667 29.705950338712405 29.114299606156603 21.309434085919325 88-89 20.672792922686426 28.726567115006347 29.45713023046968 21.143509731837547 90-91 20.758385719169407 28.711512130664357 30.040553633708317 20.48954851645792 92-93 20.304411630557713 29.429188338976324 29.944497041524926 20.321902988941034 94-95 20.12476098622023 29.48187923504963 29.755366794819675 20.637992983910465 96-97 20.905981513184056 28.804946113331603 29.522888881606974 20.766183491877367 98-99 20.984672093326022 28.614572271961187 29.876310426643123 20.524445208069668 100-101 20.554622682282258 28.6179689105221 30.025603828795315 20.801804578400322 102-103 20.267631897428583 29.238246767077708 29.599588353996083 20.89453298149763 104-105 20.55522761622153 28.704694738175252 29.50045810640202 21.2396195392012 106-107 20.658993685068243 28.12367700851143 29.48466436979107 21.732664936629263 108-109 20.24201894119038 28.84574934695094 29.27617056193438 21.6360611499243 110-111 20.077062946105535 29.111433498335366 29.636109849018265 21.17539370654083 112-113 20.454577662516186 28.397264544837764 29.366905633705514 21.78125215894054 114-115 20.118212176996764 28.797999063832542 28.592830480620577 22.490958278550117 116-117 20.61896483162417 28.693724531954786 27.99166778111275 22.69564285530829 118-119 20.572655817557845 28.171593047713937 29.099966788442376 22.155784346285838 120-121 20.175366346594963 28.27086001335551 29.57846893426928 21.975304705780243 122-123 20.623816273726003 28.6270347249991 29.025267145183236 21.723881856091655 124-125 20.73077522592896 28.66654090456605 28.558846035305496 22.043837834199497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18.0 1 16.5 2 34.0 3 50.5 4 31.0 5 26.5 6 61.0 7 133.5 8 291.5 9 372.5 10 312.5 11 259.5 12 290.5 13 390.0 14 511.0 15 619.0 16 752.5 17 918.0 18 1074.5 19 1297.0 20 1605.5 21 1993.5 22 2502.5 23 3192.5 24 4006.5 25 5151.0 26 6711.5 27 8732.5 28 10936.5 29 13470.5 30 17341.0 31 22575.0 32 28442.5 33 35487.5 34 43840.0 35 51597.0 36 57586.5 37 59511.0 38 56254.5 39 50895.5 40 46109.0 41 43276.5 42 41367.0 43 39552.0 44 38434.0 45 38112.0 46 37701.0 47 38212.0 48 40451.0 49 38256.5 50 33475.5 51 30276.0 52 26629.5 53 23853.0 54 21952.5 55 19426.5 56 16571.5 57 14141.0 58 11844.5 59 9608.0 60 7579.5 61 5764.0 62 4464.0 63 3399.5 64 2508.0 65 1938.0 66 1404.5 67 1053.0 68 743.0 69 437.5 70 305.0 71 251.5 72 210.0 73 170.0 74 130.0 75 97.5 76 53.5 77 29.0 78 23.0 79 13.0 80 6.5 81 3.5 82 2.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 3.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01505585278564274 2 0.0 3 0.0 4 0.0 5 0.11610719412928018 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0038525270363262303 22-23 2.2140959978886383E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.199468794088187E-4 92-93 0.0 94-95 8.856383991554553E-4 96-97 0.0 98-99 0.0010627660789865462 100-101 8.856383991554552E-5 102-103 3.0997343970440934E-4 104-105 0.0021698140779308653 106-107 0.005225266555017186 108-109 2.2140959978886383E-4 110-111 0.004605319675608367 112-113 0.00912207551130119 114-115 0.005092420795143868 116-117 0.0015941491184798193 118-119 3.542553596621821E-4 120-121 6.199468794088187E-4 122-123 0.00128417567877541 124-125 0.0011513299189020918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1129129.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.42570198926781 #Duplication Level Percentage of deduplicated Percentage of total 1 86.36978207561387 65.14501443713256 2 9.693488249852571 14.622763119396986 3 2.0805656759341753 4.707843799263321 4 0.7116053992716729 2.1469334711767654 5 0.32923716766160044 1.2416472245917238 6 0.18683346105455306 0.8455226973074517 7 0.11587997140865376 0.6118229732995795 8 0.08769009554647644 0.5291269611279162 9 0.062456905961421394 0.42397703785961055 >10 0.3207653280378035 4.408896121233967 >50 0.022098239536304052 1.1556890298952425 >100 0.017954536723817287 2.717314233613279 >500 0.001056145755274501 0.6313539956847 >1k 5.867476418191672E-4 0.8120948984168123 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2362 0.2091877898805185 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1989 0.17615347759202005 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1780 0.15764363504967102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1584 0.1402851224262241 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1460 0.12930320627669645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.856383991554552E-5 0.0 9 0.0 0.0 0.0 8.856383991554552E-5 0.0 10-11 0.0 0.0 0.0 8.856383991554552E-5 0.0 12-13 0.0 0.0 0.0 8.856383991554552E-5 0.0 14-15 0.0 0.0 0.0 8.856383991554552E-5 0.0 16-17 0.0 0.0 0.0 1.3284575987331827E-4 0.0 18-19 0.0 0.0 0.0 1.7712767983109104E-4 0.0 20-21 0.0 0.0 0.0 1.7712767983109104E-4 0.0 22-23 0.0 0.0 0.0 2.6569151974663655E-4 0.0 24-25 0.0 0.0 0.0 2.6569151974663655E-4 0.0 26-27 0.0 0.0 0.0 4.8710111953550035E-4 0.0 28-29 0.0 0.0 0.0 7.52792639282137E-4 0.0 30-31 0.0 0.0 0.0 0.001505585278564274 0.0 32-33 0.0 0.0 0.0 0.004605319675608367 0.0 34-35 0.0 0.0 0.0 0.008546410551850143 0.0 36-37 0.0 0.0 0.0 0.013550267507078464 0.0 38-39 0.0 0.0 0.0 0.024399337896732792 0.0 40-41 0.0 0.0 0.0 0.03768391388406462 0.0 42-43 0.0 0.0 0.0 0.05247407514996072 0.0 44-45 0.0 0.0 0.0 0.06837128441480114 0.0 46-47 0.0 0.0 0.0 0.08692540887710792 0.0 48-49 0.0 0.0 0.0 0.11070479989443191 0.0 50-51 0.0 0.0 0.0 0.14201211730457725 0.0 52-53 0.0 0.0 0.0 0.17145959407649614 0.0 54-55 0.0 0.0 0.0 0.20413965100533243 0.0 56-57 0.0 0.0 0.0 0.24053938921062165 0.0 58-59 0.0 0.0 0.0 0.2822529578108436 0.0 60-61 0.0 0.0 0.0 0.33007743136523815 0.0 62-63 0.0 0.0 0.0 0.38224153307549447 0.0 64-65 0.0 0.0 0.0 0.4329443314271443 0.0 66-67 0.0 4.428191995777276E-5 0.0 0.49431907248861734 0.0 68-69 0.0 8.856383991554552E-5 0.0 0.5718124324147197 0.0 70-71 0.0 8.856383991554552E-5 0.0 0.6585164316920387 0.0 72-73 0.0 8.856383991554552E-5 0.0 0.7439362552905824 0.0 74-75 0.0 8.856383991554552E-5 0.0 0.8267877275315751 0.0 76-77 0.0 8.856383991554552E-5 0.0 0.9208425255218846 0.0 78-79 0.0 8.856383991554552E-5 0.0 1.0328757830150497 0.0 80-81 0.0 8.856383991554552E-5 0.0 1.1527026584207827 0.0 82-83 0.0 8.856383991554552E-5 0.0 1.279924614459464 0.0 84-85 0.0 8.856383991554552E-5 0.0 1.412991783932571 0.0 86-87 0.0 8.856383991554552E-5 0.0 1.5506199911613288 0.0 88-89 0.0 8.856383991554552E-5 0.0 1.7102120306891417 0.0 90-91 0.0 8.856383991554552E-5 0.0 1.8816273428456802 0.0 92-93 0.0 8.856383991554552E-5 0.0 2.060127762195462 0.0 94-95 0.0 8.856383991554552E-5 0.0 2.2419493255420773 0.0 96-97 0.0 8.856383991554552E-5 0.0 2.426782059445821 0.0 98-99 0.0 1.7712767983109104E-4 0.0 2.6330029606891685 0.0 100-101 0.0 1.7712767983109104E-4 0.0 2.859150725913514 0.0 102-103 0.0 1.7712767983109104E-4 0.0 3.10079716312308 0.0 104-105 0.0 1.7712767983109104E-4 0.0 3.3503257820851293 0.0 106-107 0.0 1.7712767983109104E-4 0.0 3.6049025399223646 0.0 108-109 0.0 2.6569151974663655E-4 0.0 3.8680257083114507 0.0 110-111 0.0 2.6569151974663655E-4 0.0 4.159666433153342 0.0 112-113 0.0 2.6569151974663655E-4 0.0 4.482570193485421 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1515 0.0 32.624474 1 TACCTGG 1490 0.0 31.571817 2 GTATCAA 7325 0.0 29.998308 1 TAGGACC 1330 0.0 28.654121 4 GTACATG 5445 0.0 26.794535 1 TACATGG 5285 0.0 26.590454 2 GTACCGT 90 0.00953349 26.441828 6 CGCGCAG 45 0.00953547 26.441828 64-65 TATCAAC 8295 0.0 25.98669 2 GAGTACT 3495 0.0 25.874405 12-13 CAACGCA 8305 0.0 25.811998 5 TCAACGC 8305 0.0 25.811998 4 ATCAACG 8360 0.0 25.642181 3 ACATGGG 5270 0.0 25.310234 3 AACGCAG 8465 0.0 25.301687 6 AGTACTT 3725 0.0 25.235085 12-13 AGAGTAC 6695 0.0 23.904282 10-11 ACCTGGG 1920 0.0 23.880766 3 GTACTTT 3875 0.0 23.647253 14-15 GTCCTAC 4275 0.0 23.12331 1 >>END_MODULE