FastQCFastQC Report
Fri 27 May 2016
SRR939957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670316
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21950.32745749765782106No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12310.18364472875479623No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA11660.17394780968975826No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC9530.14217175183047995No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8150.12158444673855315No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7330.10935141037958217No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6860.10233979197870856No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6710.10010204142523824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCT253.0377123E-103645.6348110-111
CGTCGCG108.8268926E-43038.029110-111
CCGCTCC1850.02324.3691108-109
CGTGTCG150.00198583542025.3527110-111
CGCCGCT302.5859263E-62025.3527110-111
CCGCTCG750.01686.3069108-109
CCGCGCG456.6502253E-91686.3069108-109
CCGCGAC150.00286700951686.3069108-109
CCGTTCG150.00286700951686.3069108-109
CGTCGCC850.01487.9178108-109
CGTATTG458.724319E-61350.2351110-111
CGTGGCG200.005096241264.7301108-109
CGGCGAC250.00551501151215.2115110-111
TTCGTCG250.00551501151215.2115110-111
CTCCGCC1056.366463E-111157.3444110-111
CCTCTCG1350.01124.2046108-109
CCGCGCC1850.01093.8208108-109
CGGGTTG300.0079407541012.67633110-111
GTCGGTC300.0079407541012.67633110-111
CGCCGCC1251.5279511E-10972.1693110-111
Produced by FastQC (version 0.11.5)