##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939957_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670316 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62443981644478 33.0 31.0 34.0 30.0 34.0 2 31.816840117198456 34.0 31.0 34.0 30.0 34.0 3 31.886722083315927 34.0 31.0 34.0 30.0 34.0 4 35.177637114435576 37.0 35.0 37.0 33.0 37.0 5 35.215344703095255 37.0 35.0 37.0 33.0 37.0 6 35.27696191050191 37.0 37.0 37.0 33.0 37.0 7 35.18249154130291 37.0 37.0 37.0 33.0 37.0 8 35.199225439941756 37.0 37.0 37.0 33.0 37.0 9 36.848578879215175 39.0 38.0 39.0 34.0 39.0 10-11 36.82334302030684 39.0 38.0 39.0 34.0 39.0 12-13 36.751070390681406 39.0 38.0 39.0 33.0 39.0 14-15 38.24395807350563 41.0 39.0 41.0 33.5 41.0 16-17 38.25380343002405 41.0 39.0 41.0 33.5 41.0 18-19 38.26808475405629 41.0 39.0 41.0 33.5 41.0 20-21 38.309576378901895 41.0 39.0 41.0 34.0 41.0 22-23 38.29277087224533 41.0 39.0 41.0 34.0 41.0 24-25 38.30404316770001 41.0 39.0 41.0 34.0 41.0 26-27 38.257343551399636 41.0 39.0 41.0 33.0 41.0 28-29 38.19949098634077 41.0 39.0 41.0 33.0 41.0 30-31 38.12050510505493 41.0 39.0 41.0 33.0 41.0 32-33 38.01794750535568 40.0 38.0 41.0 33.0 41.0 34-35 37.94735691822961 40.0 38.0 41.0 33.0 41.0 36-37 38.114595951760066 41.0 39.0 41.0 33.0 41.0 38-39 38.07763129628414 41.0 39.0 41.0 33.0 41.0 40-41 38.00362739364718 41.0 39.0 41.0 33.0 41.0 42-43 37.939192112376844 41.0 38.0 41.0 33.0 41.0 44-45 37.85363171996491 41.0 38.0 41.0 33.0 41.0 46-47 37.74355751615656 40.0 38.0 41.0 32.5 41.0 48-49 37.648872322904424 40.0 38.0 41.0 32.0 41.0 50-51 37.53916660202054 40.0 38.0 41.0 32.0 41.0 52-53 37.44592475787539 40.0 38.0 41.0 32.0 41.0 54-55 37.30393426383974 40.0 38.0 41.0 31.0 41.0 56-57 37.101050101743056 40.0 37.0 41.0 31.0 41.0 58-59 36.93372528777472 40.0 37.0 41.0 31.0 41.0 60-61 36.7187617481904 40.0 36.5 41.0 30.5 41.0 62-63 36.4794291050788 40.0 36.0 41.0 30.0 41.0 64-65 36.20078216841012 39.0 35.5 41.0 30.0 41.0 66-67 35.89106033572226 39.0 35.0 41.0 29.5 41.0 68-69 35.49007855996276 38.5 35.0 40.5 29.0 41.0 70-71 35.08137281520955 37.5 35.0 40.0 28.5 41.0 72-73 34.68916749712076 37.0 35.0 39.5 28.0 41.0 74-75 34.27640993203205 36.5 34.0 39.0 28.0 41.0 76-77 33.81935385698685 36.0 34.0 39.0 27.0 40.5 78-79 33.34658504347203 36.0 34.0 38.0 26.0 39.5 80-81 32.880536940786136 35.0 34.0 37.0 26.0 39.0 82-83 32.65242288711593 35.0 34.0 37.0 26.0 39.0 84-85 32.195521962775764 35.0 34.0 36.5 24.5 38.5 86-87 31.526010120599835 35.0 34.0 36.0 10.0 37.0 88-89 16.04731052220147 18.0 16.5 18.5 2.0 19.5 90-91 2.749221262807392 2.0 2.0 2.0 2.0 2.0 92-93 2.2868811127885955 2.0 2.0 2.0 2.0 2.0 94-95 2.0965589363822437 2.0 2.0 2.0 2.0 2.0 96-97 2.0000484845953252 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.20635547873401805 1101 2 -5.695762992381503E-4 1101 3 -0.08999475308302962 1101 4 -0.0019375470817308837 1101 5 -0.1930069979465756 1101 6 -0.14397793324372543 1101 7 -0.17597912537618754 1101 8 -0.21009274120504529 1101 9 -0.19681030261335053 1101 10-11 -0.143650844371642 1101 12-13 -0.14989665966353982 1101 14-15 0.01340161021322217 1101 16-17 -0.05013844703450587 1101 18-19 -0.13100984464480803 1101 20-21 -0.0091757140378661 1101 22-23 0.04442519725866134 1101 24-25 -0.06170710675193902 1101 26-27 0.07881454415775124 1101 28-29 0.11755398784163873 1101 30-31 -0.04319516862413053 1101 32-33 -0.04306043239134283 1101 34-35 0.03298380531853695 1101 36-37 0.09013349107721069 1101 38-39 -0.10071149876254992 1101 40-41 0.034838456381031335 1101 42-43 0.0628904316577632 1101 44-45 0.009817203047063572 1101 46-47 -0.019894094003191753 1101 48-49 -0.06149184265795782 1101 50-51 -0.12642452649048863 1101 52-53 -0.12892805237404303 1101 54-55 -0.09048916990161615 1101 56-57 -0.1594151358884588 1101 58-59 -0.1965814359884135 1101 60-61 -0.18496565244373642 1101 62-63 -0.252480583541697 1101 64-65 -0.1347969761739236 1101 66-67 -0.11356787619391184 1101 68-69 -0.19240326418697862 1101 70-71 -0.2737785041959384 1101 72-73 -0.13308410543447735 1101 74-75 -0.008573598283383888 1101 76-77 -0.1654151832789239 1101 78-79 -0.12997130399828905 1101 80-81 -0.14600123215111438 1101 82-83 -0.07760315530582318 1101 84-85 -0.08925623806643301 1101 86-87 -0.029429767786378846 1101 88-89 0.49066688428513316 1101 90-91 -0.1004958258732156 1101 92-93 -0.1639052379836663 1101 94-95 0.00209952446187911 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.45280540554089654 1102 2 -0.26918656301105415 1102 3 -0.07895375666411653 1102 4 -0.2089577067951538 1102 5 -0.2645271346209981 1102 6 -0.33328051325698027 1102 7 -0.11219182139965511 1102 8 -0.11895755663904595 1102 9 -0.11970763965970832 1102 10-11 -0.019677999669845292 1102 12-13 0.033611907889756765 1102 14-15 -0.060241067934349246 1102 16-17 -0.1051183558473312 1102 18-19 -0.22557311386671586 1102 20-21 -0.20884643359892152 1102 22-23 -0.1613815752613661 1102 24-25 -0.13957360107903583 1102 26-27 -0.1757073021284299 1102 28-29 -0.21435466903208322 1102 30-31 -0.03868129737550419 1102 32-33 -0.13775720964913063 1102 34-35 -0.19929359366136623 1102 36-37 -0.09808625606093102 1102 38-39 -0.012794684902289077 1102 40-41 0.010871991709272777 1102 42-43 0.019057853097734778 1102 44-45 0.0503156251711232 1102 46-47 0.06267826321705172 1102 48-49 0.011681194389929317 1102 50-51 0.05833169138044525 1102 52-53 0.045111487271341844 1102 54-55 0.03756216807338575 1102 56-57 -0.0999928357931168 1102 58-59 -0.24628954961438154 1102 60-61 -0.10287002524543709 1102 62-63 -0.040471004044761116 1102 64-65 -0.07020626100800342 1102 66-67 -0.15980300865632557 1102 68-69 -0.005854730471476444 1102 70-71 -0.02114821866597083 1102 72-73 -0.08195111843438241 1102 74-75 0.02490088336872276 1102 76-77 -0.02018088849081323 1102 78-79 0.06791832384510599 1102 80-81 0.22674788734565965 1102 82-83 0.28695412377677343 1102 84-85 0.2106778349565559 1102 86-87 0.2740529015709292 1102 88-89 1.0472240783006654 1102 90-91 -0.1135087241672661 1102 92-93 -0.10210873967496514 1102 94-95 0.0452196663348432 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.12120357114145364 1103 2 0.08023981612784681 1103 3 -0.08435603333785835 1103 4 -0.04593955573918862 1103 5 0.003968984347821447 1103 6 -0.11644737533567451 1103 7 -0.1440118246647657 1103 8 -0.10511051230772495 1103 9 -0.0033954355605061437 1103 10-11 -0.12807972721106609 1103 12-13 -0.004285656633321366 1103 14-15 -0.04229037112970957 1103 16-17 -0.016047577118051493 1103 18-19 0.006850935169332217 1103 20-21 0.024987550283292137 1103 22-23 0.14036674394788662 1103 24-25 0.11394010497181739 1103 26-27 0.026380885585844283 1103 28-29 0.050772447725371705 1103 30-31 -0.0013402860811808637 1103 32-33 0.10499646731253165 1103 34-35 -0.14554539318346826 1103 36-37 -0.01792032899166429 1103 38-39 -0.08197054088115863 1103 40-41 -0.11441927114710637 1103 42-43 -0.14656496706878386 1103 44-45 -0.16964163154091239 1103 46-47 -0.16621352799385392 1103 48-49 -0.21266170176465948 1103 50-51 8.953981733625938E-4 1103 52-53 -0.22843071430481388 1103 54-55 -0.219023711800169 1103 56-57 -0.19961586373950269 1103 58-59 -0.13690086768084342 1103 60-61 -0.2552542992774036 1103 62-63 -0.3062363018378349 1103 64-65 -0.19385012630727516 1103 66-67 -0.12192770626241156 1103 68-69 -0.10671701694055713 1103 70-71 -0.04376654188239826 1103 72-73 0.046449421793290924 1103 74-75 -0.14771966606063103 1103 76-77 -0.04377132468012945 1103 78-79 -0.13227758289141178 1103 80-81 -0.08988637555523837 1103 82-83 -0.07838225699467927 1103 84-85 -0.03027260954395672 1103 86-87 0.03443818481941818 1103 88-89 0.6022709029789759 1103 90-91 -0.5645543021663464 1103 92-93 -0.32421058149511683 1103 94-95 -0.11458531741380229 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.05339610310241838 1104 2 0.13441411043271145 1104 3 0.22965979208884235 1104 4 0.23258816880310462 1104 5 0.19788733338711495 1104 6 0.24821346392271693 1104 7 0.31959441536923094 1104 8 0.2250150996023379 1104 9 0.1437827496446289 1104 10-11 0.1622301498382015 1104 12-13 0.23326635974580512 1104 14-15 0.24054138016171578 1104 16-17 0.23549976932229555 1104 18-19 0.22085313278758179 1104 20-21 0.23373928595102456 1104 22-23 0.1358411311793759 1104 24-25 0.19659222672501642 1104 26-27 0.29435456996580456 1104 28-29 0.2880631750211151 1104 30-31 0.19090355940736714 1104 32-33 0.40350556360640866 1104 34-35 0.26380821947343236 1104 36-37 0.249206177846915 1104 38-39 0.182480837888221 1104 40-41 0.31761713119730217 1104 42-43 0.2553148120534132 1104 44-45 0.2313383903065045 1104 46-47 0.2072662202243336 1104 48-49 0.23128079102976784 1104 50-51 0.29591920928120174 1104 52-53 0.2420838588657901 1104 54-55 0.24130648892564466 1104 56-57 0.20388101044898832 1104 58-59 0.2362532081069375 1104 60-61 0.3049065732457166 1104 62-63 0.15103515337603568 1104 64-65 0.1440760646236825 1104 66-67 0.11286172800112126 1104 68-69 0.2290120922729173 1104 70-71 0.22495774501837928 1104 72-73 0.2632539045911315 1104 74-75 0.17129767237472748 1104 76-77 0.14234644533826213 1104 78-79 0.035403479176281394 1104 80-81 0.05386017725304981 1104 82-83 0.12854313772889014 1104 84-85 0.09286268776761375 1104 86-87 0.09301469047763788 1104 88-89 0.905407015812937 1104 90-91 -0.5785523148670344 1104 92-93 -0.32421058149511683 1104 94-95 -0.11458531741380229 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.09150078201123435 1105 2 -0.0036127634920113394 1105 3 0.07987791648944409 1105 4 0.14703493167888837 1105 5 0.20113475531688607 1105 6 0.16092001639218978 1105 7 0.2598894918115846 1105 8 -0.020902739166601236 1105 9 0.21522187140526938 1105 10-11 0.2347063246622625 1105 12-13 0.2985986223937971 1105 14-15 0.23841422518209754 1105 16-17 0.34222105897111277 1105 18-19 0.3617586871064873 1105 20-21 0.3355031082976936 1105 22-23 0.14631515908515524 1105 24-25 0.2629459398326617 1105 26-27 0.32715176316140315 1105 28-29 0.25173771302801384 1105 30-31 0.22652066318964614 1105 32-33 0.23986129465080808 1105 34-35 0.3512836973811915 1105 36-37 0.12570324767793295 1105 38-39 0.19450940521553406 1105 40-41 0.2730909406666271 1105 42-43 0.45915138830126523 1105 44-45 0.16057694163595215 1105 46-47 0.35672928262018644 1105 48-49 0.41882824894955206 1105 50-51 0.2627241496896602 1105 52-53 0.25796106845452016 1105 54-55 0.29661771926376446 1105 56-57 0.28142595114869806 1105 58-59 0.3603479970453165 1105 60-61 0.4347150356819256 1105 62-63 0.3490618400695098 1105 64-65 0.34584113517865944 1105 66-67 0.24056019832185171 1105 68-69 0.2327629582174211 1105 70-71 0.3127078287523517 1105 72-73 0.18852575395735727 1105 74-75 0.27174827557139025 1105 76-77 0.29392730115148424 1105 78-79 0.4283248899013046 1105 80-81 0.4367262299399286 1105 82-83 0.3561991689673789 1105 84-85 0.3006019953032535 1105 86-87 0.32877575428396355 1105 88-89 0.892529666840943 1105 90-91 -0.7689800134413725 1105 92-93 -0.32421058149511683 1105 94-95 -0.11458531741380229 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.17952065625843616 1106 2 0.023566524853634974 1106 3 0.024236705772008094 1106 4 -0.16006835022422905 1106 5 -0.07824055028306987 1106 6 -0.09257685699755314 1106 7 -0.06984058813285543 1106 8 0.03550278780161875 1106 9 -0.06974559706794992 1106 10-11 -0.07472483170267452 1106 12-13 -0.2247540230009193 1106 14-15 -0.08825707125011917 1106 16-17 -0.08493757765067045 1106 18-19 -0.15627158386640616 1106 20-21 0.012588603397460929 1106 22-23 -0.1721733979363833 1106 24-25 -0.1083062531475747 1106 26-27 -0.20413509232005822 1106 28-29 0.07306183272297773 1106 30-31 0.15242754213713994 1106 32-33 0.14619104801455762 1106 34-35 0.058905904463316006 1106 36-37 0.11509747390137193 1106 38-39 0.04671898781786865 1106 40-41 0.13508022809104858 1106 42-43 -0.14805889787696458 1106 44-45 0.11536130828098123 1106 46-47 -0.05330765764470158 1106 48-49 0.050427816073217 1106 50-51 -0.030164368458038382 1106 52-53 -0.05612634300374708 1106 54-55 0.04571952112667077 1106 56-57 -0.01737614763335671 1106 58-59 0.006918039290233935 1106 60-61 0.18325903853267533 1106 62-63 0.09819755530248386 1106 64-65 -0.004340225425750077 1106 66-67 -0.012164260101876323 1106 68-69 0.033225293623694085 1106 70-71 -0.16618793167145185 1106 72-73 -0.11887718040964756 1106 74-75 -0.1880385662434918 1106 76-77 -0.07973375344297295 1106 78-79 0.05123048621477011 1106 80-81 0.11438268446412536 1106 82-83 0.033521045318224196 1106 84-85 0.04143450273635807 1106 86-87 0.35833606491019054 1106 88-89 0.8320297331491737 1106 90-91 -0.14806821958607053 1106 92-93 -0.2959647935862959 1106 94-95 -0.11458531741380229 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.10803762440767883 1107 2 -0.03946598198302809 1107 3 -0.046987869837874285 1107 4 0.0628620862785425 1107 5 0.050549188606538564 1107 6 0.054935054355929935 1107 7 0.05605941117129731 1107 8 0.15958663498240355 1107 9 0.1390741227456047 1107 10-11 0.0817177368354507 1107 12-13 0.08702621364559349 1107 14-15 0.08548864558323999 1107 16-17 -0.021672460197216026 1107 18-19 0.19353850049967747 1107 20-21 0.03300316720782348 1107 22-23 0.13707446458079886 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0.14408638246771943 2216 2 0.08672753633124941 2216 3 0.0802627617248639 2216 4 0.1749743566997637 2216 5 0.09443853298823512 2216 6 0.09846065189543651 2216 7 0.024048024313628957 2216 8 0.05932901565138593 2216 9 0.06041490858860499 2216 10-11 0.012359870705935805 2216 12-13 0.09048805144113459 2216 14-15 0.10123130925890678 2216 16-17 0.03838447409112433 2216 18-19 -0.029790823572128033 2216 20-21 0.0051715395447544665 2216 22-23 0.1414681435430012 2216 24-25 -0.009256620998741028 2216 26-27 -0.07125672377802061 2216 28-29 0.06910491387230167 2216 30-31 -0.04445446173671286 2216 32-33 0.023374406090631794 2216 34-35 0.040603818259889124 2216 36-37 -0.04047868917862729 2216 38-39 -0.042565008086207 2216 40-41 -0.05297088039728948 2216 42-43 -0.10927434885961418 2216 44-45 -0.1696499390408377 2216 46-47 -0.11177316928475989 2216 48-49 0.009344865532646907 2216 50-51 0.07145055778666887 2216 52-53 -0.10390916071488476 2216 54-55 -0.14842570297672353 2216 56-57 -0.09622378034969614 2216 58-59 0.01854863365053916 2216 60-61 -0.18316924796766187 2216 62-63 -0.1447026499045876 2216 64-65 -0.14091561126851815 2216 66-67 -0.1943988377001773 2216 68-69 -0.2759333573556688 2216 70-71 -0.1766563713163123 2216 72-73 -0.18711244066074784 2216 74-75 -0.3267865152008014 2216 76-77 -0.20030298718575779 2216 78-79 -0.17038452227726708 2216 80-81 -0.16942281705951245 2216 82-83 -0.23582249764984198 2216 84-85 -0.29898306693741006 2216 86-87 -0.3522545120136762 2216 88-89 -1.20293483881518 2216 90-91 -0.7689800134413725 2216 92-93 -0.32421058149511683 2216 94-95 -0.11458531741380229 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10041.0 3 1634.0 4 1042.0 5 877.0 6 843.0 7 896.0 8 1073.0 9 1201.0 10 1451.0 11 1664.0 12 1969.0 13 2304.0 14 2601.0 15 2896.0 16 3286.0 17 3911.0 18 5155.0 19 6317.0 20 6773.0 21 9646.0 22 11609.0 23 14263.0 24 21676.0 25 35951.0 26 65420.0 27 178888.0 28 266836.0 29 9727.0 30 366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.558011445348164 20.03174622118523 14.703065419891514 23.70717691357509 2 13.145033521603308 20.289446438534775 44.75797909636379 21.807540943498122 3 19.489401964251872 25.457372809393547 29.01576982943751 26.03745539691707 4 11.420964104645904 17.38009136890681 39.810546079211264 31.38839844723602 5 12.330676696218024 38.5995940419689 34.806274439568966 14.263454822244112 6 29.320947135380926 37.156206923301845 20.113797074812474 13.409048866504753 7 28.003210426127378 31.83871487477548 22.203110174902584 17.95496452419456 8 23.809069155443105 38.79782072932766 20.72127175839455 16.671838356834687 9 25.374301075910466 18.768163075325667 22.07346982617154 33.78406602259233 10-11 23.90546747843159 26.916317698255583 29.8178899582133 19.36032486509953 12-13 25.99979263062965 24.44989307050633 29.098249071499914 20.452065227364105 14-15 20.746589290109874 28.993085340474256 26.18932889760784 24.07099647180803 16-17 20.25019692322381 30.476628793755744 29.28501951330127 19.988154769719177 18-19 21.08614567481276 28.895130247963476 30.107868588309017 19.91085548891475 20-21 21.978921205529808 28.64002315400171 28.297860119693656 21.083195520774822 22-23 20.811104745878016 30.243634269619342 28.527632073613923 20.417628910888727 24-25 21.513957596118846 28.688931787395795 29.89313995190328 19.903970664582076 26-27 20.11096259077808 29.936179354215025 29.436862614050685 20.515995440956207 28-29 19.861108003073227 30.497460111441804 27.883426201505284 21.758005683979682 30-31 20.80958831357151 28.856837670591183 29.081284051104255 21.252289964733052 32-33 20.113724179703002 29.762302590160317 29.781995088810188 20.3419781413265 34-35 20.596719007691156 29.94134851424441 29.475896051933752 19.986036426130678 36-37 19.96353950272596 29.34070499743031 29.594839437308906 21.100916062534825 38-39 20.822119716670944 30.120346224765633 28.674759367223817 20.382774691339606 40-41 22.24421019340132 28.380942719553165 29.601486463100983 19.773360623944527 42-43 20.03107489601919 30.184495073965117 29.477291307383386 20.307138722632313 44-45 19.942221108316403 30.085452489430324 28.891832782845057 21.080493619408216 46-47 21.430937050585097 28.98662720269246 28.76717846508214 20.8152572816403 48-49 20.739549479459985 29.289131130433745 28.812786852718915 21.158532537387355 50-51 19.574155434385325 30.449415573275253 29.23110776276825 20.74532122957117 52-53 19.833406930462647 29.735602312938976 29.484302925784256 20.946687830814124 54-55 20.020363940161346 28.90844059212072 29.781703037061625 21.28949243065631 56-57 20.716796257287605 29.168556322689597 28.121139880295264 21.99350753972753 58-59 20.996738851526743 29.746268923910513 27.70969214519719 21.547300079365552 60-61 20.294607319532876 28.348495336527847 30.42923039282965 20.927666951109625 62-63 20.63981763824823 28.39556268983584 29.890007101128425 21.074612570787508 64-65 20.941317229485794 29.554419109792995 28.293670447967823 21.210593212753388 66-67 20.888207949683434 30.355608399620476 27.69365493289732 21.06252871779877 68-69 21.487557687559626 28.556414274813203 29.472254400366392 20.483773637260775 70-71 22.265543415344403 28.367516216232346 28.77874017627507 20.588200192148182 72-73 21.261688517057625 29.527715286521584 28.561902147643796 20.648694048776996 74-75 20.48921702599968 30.23260671086473 29.286038226746786 19.992138036388805 76-77 20.890072741811323 28.64969656102495 29.79452974418036 20.665700952983368 78-79 20.53155645596209 28.69652976326784 29.330336729997637 21.441577050772437 80-81 21.124141449704318 29.302970539268046 28.963130821881023 20.60975718914661 82-83 20.62991703098788 29.155592818552 30.081134041612057 20.133356108848062 84-85 20.468629720907753 29.17161458177934 29.865168069984904 20.494587627328006 86-87 19.58824867691321 30.68763216881804 28.353181189230312 21.370937965038433 88-89 29.334962268541602 18.711054227659393 35.03531206483236 16.918671438966644 90-91 30.261065867246007 14.99338536040683 16.77539354071341 37.970155231633754 92-93 22.22129143978845 17.52664712023556 35.756090547070855 24.495970892905145 94-95 15.183699996224604 6.846006979089698 27.076737626138687 50.89355539854701 96-97 26.18068478125606 7.117245131547342 14.606226178658973 52.09584390853763 98-99 57.29765139230447 14.058355437665782 18.670733147907516 9.973260022122231 100-101 30.424503171149414 7.096518144348683 12.533877326629161 49.945101357872744 102-103 22.831826027473532 1.9656222906276597 36.88309828906865 38.319453392830155 104-105 41.31571997851741 4.600361406101175 40.58875368304863 13.49516493233279 106-107 5.260553153167772 1.6226258954866661 6.079167917440794 87.03765303390477 108-109 17.593130008159864 10.747037354428349 21.279664865123234 50.38016777228855 110-111 39.6511947199898 2.6778840731787446 24.769784102108964 32.90113710472249 112-113 27.130782330116716 2.7291742805377788 38.26582583610025 31.87421755324526 114-115 27.873605443714748 3.8047225581842223 23.160655144739966 45.16101685336106 116-117 18.94545163128651 4.088011112694116 19.572989385952415 57.39354787006696 118-119 19.596064078081902 4.1929238277105885 14.914067472947167 61.29694462126034 120-121 24.818312157017846 4.092777741860651 29.22711836045499 41.86179174066651 122-123 33.66269659867307 1.6206649452056485 33.18394099259937 31.532697463521913 124-125 37.99495782254403 1.9335316749109852 19.36623294381915 40.70527755872583 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 13.5 2 36.0 3 59.0 4 73.5 5 81.0 6 78.5 7 75.5 8 63.5 9 58.0 10 68.5 11 70.5 12 68.5 13 73.0 14 83.0 15 107.0 16 147.5 17 205.5 18 313.0 19 467.0 20 655.5 21 897.0 22 1284.0 23 1861.5 24 2652.5 25 3585.5 26 4673.0 27 6132.0 28 7962.0 29 10073.5 30 12657.0 31 15908.0 32 19929.0 33 24120.5 34 27890.5 35 30913.0 36 32270.0 37 32178.0 38 31535.0 39 30461.5 40 29271.5 41 28031.0 42 26721.5 43 25953.5 44 25492.5 45 24669.5 46 23270.5 47 21919.0 48 20734.5 49 19440.0 50 18392.5 51 16995.5 52 15065.5 53 13149.5 54 11372.0 55 9747.0 56 8296.5 57 6925.5 58 5720.0 59 4680.0 60 3690.0 61 2879.0 62 2202.0 63 1633.5 64 1214.5 65 900.0 66 640.0 67 438.0 68 308.0 69 226.5 70 148.5 71 93.5 72 67.5 73 55.0 74 47.0 75 41.0 76 29.0 77 14.0 78 9.0 79 7.0 80 6.5 81 6.0 82 4.0 83 3.5 84 3.0 85 2.5 86 2.5 87 1.5 88 2.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.5 94 1.0 95 1.0 96 0.5 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 2.9836674046270715E-4 3 0.0356548254852935 4 0.04251726051593577 5 0.044158277588480656 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.001044283591619475 12-13 0.0021631588683546266 14-15 1.4918337023135358E-4 16-17 5.967334809254143E-4 18-19 0.0083542687329558 20-21 0.003207442459974102 22-23 0.0011934669618508286 24-25 0.0 26-27 0.0 28-29 0.0016410170725448892 30-31 0.0 32-33 0.0014918337023135356 34-35 7.459168511567679E-5 36-37 2.2377505534703038E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.9836674046270715E-4 46-47 0.0 48-49 3.729584255783839E-4 50-51 1.4918337023135358E-4 52-53 0.0 54-55 0.002013975498123273 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.729584255783839E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.2377505534703038E-4 80-81 0.0 82-83 8.205085362724446E-4 84-85 0.0 86-87 0.0012680586469665054 88-89 19.223470721271756 90-91 26.25000745916851 92-93 40.34119728605613 94-95 44.679524284069004 96-97 48.451029066887855 98-99 93.0540968737133 100-101 51.69763216154769 102-103 53.11129377785999 104-105 74.86133405736996 106-107 81.61210533539406 108-109 12.609276818694468 110-111 39.151385316775965 112-113 52.03642759534309 114-115 86.80189641900238 116-117 66.49222157907614 118-119 71.87595104398522 120-121 80.38604180714768 122-123 75.85049439368895 124-125 50.056988047428376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 670316.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.37261332476048 #Duplication Level Percentage of deduplicated Percentage of total 1 90.9236229582356 74.89616436025055 2 6.8121142617481185 11.22263308014128 3 1.1753413345994823 2.9044781183871327 4 0.3975867040843044 1.310010233544095 5 0.19484761856320434 0.8025053770578627 6 0.10885469651678635 0.5379987494856836 7 0.07821229109195695 0.45097855679529647 8 0.05122999381615155 0.33759587769981775 9 0.035121650602302636 0.26037559299517343 >10 0.19191618909245117 2.9001640730189227 >50 0.016844161202818817 0.9634856160136587 >100 0.012315867728398149 1.9054513172437146 >500 0.0014489256133659206 0.8230797768644904 >1k 5.433471050122202E-4 0.6850792705023999 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2195 0.32745749765782106 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1231 0.18364472875479623 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1166 0.17394780968975826 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 953 0.14217175183047995 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 815 0.12158444673855315 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 733 0.10935141037958217 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 686 0.10233979197870856 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 671 0.10010204142523824 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.4918337023135358E-4 0.0 0.0 0.0 0.0 8 1.4918337023135358E-4 0.0 0.0 0.0 0.0 9 1.4918337023135358E-4 0.0 0.0 1.4918337023135358E-4 0.0 10-11 1.4918337023135358E-4 0.0 0.0 1.4918337023135358E-4 0.0 12-13 1.4918337023135358E-4 0.0 0.0 1.4918337023135358E-4 1.4918337023135358E-4 14-15 1.4918337023135358E-4 0.0 0.0 1.4918337023135358E-4 1.4918337023135358E-4 16-17 1.4918337023135358E-4 0.0 0.0 1.4918337023135358E-4 1.4918337023135358E-4 18-19 1.4918337023135358E-4 0.0 0.0 1.4918337023135358E-4 1.4918337023135358E-4 20-21 1.4918337023135358E-4 0.0 0.0 2.2377505534703035E-4 1.4918337023135358E-4 22-23 4.475501106940607E-4 0.0 0.0 3.729584255783839E-4 1.4918337023135358E-4 24-25 5.967334809254143E-4 7.459168511567679E-5 0.0 4.475501106940607E-4 1.4918337023135358E-4 26-27 6.713251660410911E-4 1.4918337023135358E-4 0.0 5.221417958097375E-4 1.4918337023135358E-4 28-29 7.459168511567679E-4 1.4918337023135358E-4 0.0 0.001044283591619475 1.4918337023135358E-4 30-31 7.459168511567679E-4 1.4918337023135358E-4 0.0 0.00231234223858598 1.4918337023135358E-4 32-33 7.459168511567679E-4 1.4918337023135358E-4 0.0 0.005221417958097375 1.4918337023135358E-4 34-35 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.009920694120385012 1.4918337023135358E-4 36-37 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.017678229372415397 1.4918337023135358E-4 38-39 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.030060449101617745 1.4918337023135358E-4 40-41 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.04669439488241367 1.4918337023135358E-4 42-43 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.06638659975295234 1.4918337023135358E-4 44-45 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.08615339630860669 1.4918337023135358E-4 46-47 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.10912763532423514 1.4918337023135358E-4 48-49 8.951002213881214E-4 1.4918337023135358E-4 0.0 0.13635360039145716 2.9836674046270715E-4 50-51 0.001044283591619475 1.4918337023135358E-4 0.0 0.16895016678700792 2.9836674046270715E-4 52-53 0.001044283591619475 1.4918337023135358E-4 0.0 0.19915979925885702 2.9836674046270715E-4 54-55 0.001044283591619475 1.4918337023135358E-4 0.0 0.23421789126322512 2.9836674046270715E-4 56-57 0.001044283591619475 1.4918337023135358E-4 0.0 0.27584005155777275 2.9836674046270715E-4 58-59 0.001044283591619475 1.4918337023135358E-4 0.0 0.3206696543122945 2.9836674046270715E-4 60-61 0.001044283591619475 1.4918337023135358E-4 0.0 0.37213791704211147 2.9836674046270715E-4 62-63 0.001044283591619475 1.4918337023135358E-4 0.0 0.42658984717655557 2.9836674046270715E-4 64-65 0.001044283591619475 1.4918337023135358E-4 0.0 0.48148932742169365 4.475501106940607E-4 66-67 0.001044283591619475 1.4918337023135358E-4 0.0 0.5515309197453142 4.475501106940607E-4 68-69 0.001044283591619475 1.4918337023135358E-4 0.0 0.6355211571855661 4.475501106940607E-4 70-71 0.001044283591619475 1.4918337023135358E-4 0.0 0.7284623968396995 4.475501106940607E-4 72-73 0.001044283591619475 1.4918337023135358E-4 0.0 0.8181961940338587 5.221417958097375E-4 74-75 0.001044283591619475 1.4918337023135358E-4 0.0 0.909720191670794 5.967334809254143E-4 76-77 0.001044283591619475 1.4918337023135358E-4 0.0 1.0137755924071632 5.967334809254143E-4 78-79 0.001044283591619475 1.4918337023135358E-4 0.0 1.0418966576957733 5.967334809254143E-4 80-81 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 82-83 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 84-85 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 86-87 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 88-89 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 90-91 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 92-93 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 94-95 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 96-97 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 98-99 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 100-101 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 102-103 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 104-105 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 106-107 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 108-109 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 110-111 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 112-113 0.001044283591619475 1.4918337023135358E-4 0.0 1.0423442078064673 5.967334809254143E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGCT 25 3.0377123E-10 3645.6348 110-111 CGTCGCG 10 8.8268926E-4 3038.029 110-111 CCGCTCC 185 0.0 2324.3691 108-109 CGTGTCG 15 0.0019858354 2025.3527 110-111 CGCCGCT 30 2.5859263E-6 2025.3527 110-111 CCGCTCG 75 0.0 1686.3069 108-109 CCGCGCG 45 6.6502253E-9 1686.3069 108-109 CCGCGAC 15 0.0028670095 1686.3069 108-109 CCGTTCG 15 0.0028670095 1686.3069 108-109 CGTCGCC 85 0.0 1487.9178 108-109 CGTATTG 45 8.724319E-6 1350.2351 110-111 CGTGGCG 20 0.00509624 1264.7301 108-109 CGGCGAC 25 0.0055150115 1215.2115 110-111 TTCGTCG 25 0.0055150115 1215.2115 110-111 CTCCGCC 105 6.366463E-11 1157.3444 110-111 CCTCTCG 135 0.0 1124.2046 108-109 CCGCGCC 185 0.0 1093.8208 108-109 CGGGTTG 30 0.007940754 1012.67633 110-111 GTCGGTC 30 0.007940754 1012.67633 110-111 CGCCGCC 125 1.5279511E-10 972.1693 110-111 >>END_MODULE