FastQCFastQC Report
Fri 27 May 2016
SRR939957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670316
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25790.38474391182666084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15460.23063749037767262No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA11900.17752821057531074No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11350.1693231252125863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.16768210814004142No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC9280.1384421675746961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.11561711192929901No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7720.11516956181860495No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7280.10860549352842541No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7000.1044283591619475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6950.042.005572
GTATACG650.001924271836.6696851
GTACCTG8350.034.9679451
TACATGG29000.030.611452
GTACATG30200.030.188741
TAGGACC10100.030.0846144
ACATGGG28650.029.3217643
ACCTGGG9400.028.522023
GTAGCCC3400.028.0373333
GTGTAGC4500.027.8078441
GTGTAGG3250.027.5022621
TAGCCCT6300.027.4254064
AGTACTT22800.027.39896412-13
AGGACCG900.00946450326.4797025
AGAGTAC37400.026.16820510-11
GAGTACT22350.025.55485212-13
CATGGGG17000.024.8831334
TAAGACG1451.327201E-424.6535174
CCTAGAC2951.7826096E-1024.235663
GTACTTT24000.024.04764614-15
Produced by FastQC (version 0.11.5)