##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939957_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670316 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.830869321335015 34.0 31.0 34.0 31.0 34.0 2 32.8958610565763 34.0 31.0 34.0 31.0 34.0 3 32.941341098825035 34.0 31.0 34.0 31.0 34.0 4 36.35219806777699 37.0 37.0 37.0 35.0 37.0 5 36.25907184074377 37.0 37.0 37.0 35.0 37.0 6 36.3950823193837 37.0 37.0 37.0 35.0 37.0 7 36.38847051241504 37.0 37.0 37.0 35.0 37.0 8 36.40819852129444 37.0 37.0 37.0 35.0 37.0 9 38.24950918671194 39.0 39.0 39.0 37.0 39.0 10-11 38.23218825151123 39.0 39.0 39.0 37.0 39.0 12-13 38.21667318100717 39.0 39.0 39.0 37.0 39.0 14-15 39.74248339589089 41.0 40.0 41.0 37.0 41.0 16-17 39.757543457115744 41.0 40.0 41.0 37.0 41.0 18-19 39.80529854576051 41.0 40.0 41.0 37.5 41.0 20-21 39.832443951807804 41.0 40.0 41.0 38.0 41.0 22-23 39.78540941884126 41.0 40.0 41.0 37.0 41.0 24-25 39.76083742593046 41.0 40.0 41.0 37.0 41.0 26-27 39.66967519796633 41.0 40.0 41.0 37.0 41.0 28-29 39.595321460326176 41.0 40.0 41.0 37.0 41.0 30-31 39.4852002637562 41.0 39.5 41.0 36.5 41.0 32-33 39.3498872173721 41.0 39.0 41.0 36.0 41.0 34-35 39.255751764839275 41.0 39.0 41.0 35.0 41.0 36-37 39.256535723449836 41.0 39.0 41.0 35.0 41.0 38-39 39.31441364968164 41.0 40.0 41.0 35.0 41.0 40-41 39.24222232499299 41.0 40.0 41.0 35.0 41.0 42-43 39.15818061929001 41.0 40.0 41.0 35.0 41.0 44-45 39.026773939455424 41.0 39.0 41.0 35.0 41.0 46-47 38.83196208952196 41.0 39.0 41.0 35.0 41.0 48-49 38.62837005233352 41.0 39.0 41.0 35.0 41.0 50-51 38.48248811008539 41.0 39.0 41.0 35.0 41.0 52-53 38.39340773605285 41.0 39.0 41.0 35.0 41.0 54-55 38.295318178292035 41.0 39.0 41.0 35.0 41.0 56-57 38.156674613167525 41.0 38.5 41.0 34.5 41.0 58-59 37.998339589089326 40.5 38.0 41.0 34.0 41.0 60-61 37.81799255873349 40.0 38.0 41.0 34.0 41.0 62-63 37.621512092804 40.0 37.0 41.0 34.0 41.0 64-65 37.39187338509002 40.0 37.0 41.0 33.5 41.0 66-67 37.12384830438181 39.0 36.0 41.0 33.0 41.0 68-69 36.78737341791036 39.0 36.0 41.0 33.0 41.0 70-71 36.414339654730014 38.5 35.0 40.5 32.0 41.0 72-73 36.062950160819675 37.0 35.0 40.0 32.0 41.0 74-75 35.70556498725974 37.0 35.0 39.0 32.0 41.0 76-77 35.31666109715418 36.5 35.0 39.0 31.5 41.0 78-79 34.932967436253946 36.0 35.0 39.0 31.0 40.0 80-81 34.63048994802452 36.0 35.0 37.0 31.0 39.0 82-83 34.37080869321335 35.0 35.0 37.0 31.0 39.0 84-85 34.0817323471318 35.0 35.0 37.0 31.0 39.0 86-87 33.84916069435908 35.0 35.0 36.0 31.0 37.0 88-89 33.61816218022545 35.0 35.0 36.0 31.0 37.0 90-91 33.419476038167076 35.0 35.0 36.0 31.0 37.0 92-93 33.27268109369312 35.0 34.5 35.0 31.0 36.0 94-95 33.16566067347341 35.0 34.0 35.0 31.0 36.0 96-97 33.046320690539986 35.0 34.0 35.0 31.0 36.0 98-99 32.941301565231925 35.0 34.0 35.0 30.0 36.0 100-101 32.91417406118905 35.0 34.0 35.0 30.0 36.0 102-103 32.06918304202794 34.5 33.0 35.0 28.0 35.0 104-105 32.74202092744318 35.0 34.0 35.0 30.0 35.0 106-107 32.80217464598786 35.0 34.0 35.0 30.0 35.0 108-109 32.81152993513507 35.0 34.0 35.0 30.0 35.0 110-111 32.79348471467189 35.0 34.0 35.0 30.0 35.0 112-113 32.719911206058036 35.0 34.0 35.0 30.0 35.0 114-115 32.65898024215444 35.0 34.0 35.0 30.0 35.0 116-117 32.60258818229015 35.0 34.0 35.0 30.0 35.0 118-119 32.52500387876763 35.0 34.0 35.0 29.5 35.0 120-121 32.43097882192876 35.0 34.0 35.0 29.0 35.0 122-123 32.27249536039719 35.0 34.0 35.0 29.0 35.0 124-125 30.237957172438072 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11615467836374194 1101 2 0.14045311232899138 1101 3 0.1250994179472258 1101 4 0.06440001603234435 1101 5 0.09115723858648295 1101 6 0.17123010864617783 1101 7 0.0875806537429682 1101 8 0.13464753832245435 1101 9 0.08018955539933614 1101 10-11 0.11634672171228289 1101 12-13 0.06434148582389554 1101 14-15 0.17411352250491774 1101 16-17 0.2182621516978287 1101 18-19 0.2941432739357026 1101 20-21 0.2792449849776233 1101 22-23 0.2910119927736474 1101 24-25 0.2565036102490481 1101 26-27 0.23875556359718075 1101 28-29 0.21485723184093075 1101 30-31 0.1925733130770837 1101 32-33 0.21247402346623545 1101 34-35 0.2128943339931837 1101 36-37 0.24023943580282037 1101 38-39 0.19304949553988138 1101 40-41 0.34589288569888055 1101 42-43 9.480801565331376E-5 1101 44-45 -0.039433537851017775 1101 46-47 -0.04847150671417211 1101 48-49 -0.041844492102427466 1101 50-51 -0.08058773307122635 1101 52-53 -0.1005547016844659 1101 54-55 -0.1218582584233232 1101 56-57 0.05041869007077793 1101 58-59 -0.029918694427891523 1101 60-61 0.07465742629224081 1101 62-63 0.09735579430788732 1101 64-65 0.03957688249020208 1101 66-67 0.03803182961758722 1101 68-69 0.003189799157311768 1101 70-71 0.13427404087174466 1101 72-73 0.12067181143115135 1101 74-75 0.20423518014012387 1101 76-77 0.1106919573213645 1101 78-79 0.14038124350344106 1101 80-81 0.08482738848780258 1101 82-83 0.0828269786405329 1101 84-85 0.06636446643383209 1101 86-87 0.08201192003654256 1101 88-89 0.1302272374210105 1101 90-91 0.1169898057140557 1101 92-93 0.12634447201025267 1101 94-95 0.07979818249603454 1101 96-97 0.13546050521204478 1101 98-99 0.31158250110225083 1101 100-101 0.2269847721155358 1101 102-103 0.2177610889960988 1101 104-105 0.2189762211100117 1101 106-107 0.19394757640898064 1101 108-109 0.16197188040400334 1101 110-111 0.2162405736575863 1101 112-113 0.221645788189484 1101 114-115 0.25571585540359365 1101 116-117 0.1867939703426984 1101 118-119 0.19827000268857375 1101 120-121 0.1397719326135416 1101 122-123 0.22264391271315986 1101 124-125 0.40820988402188974 1102 1 -0.0016762059438661936 1102 2 0.05559871288475904 1102 3 0.07252797047200232 1102 4 0.007457066497195797 1102 5 0.04543132703969377 1102 6 0.02227294818487735 1102 7 0.04757550245946618 1102 8 -0.05853150067961366 1102 9 -0.011932170847750001 1102 10-11 0.09938824743738195 1102 12-13 0.017454763257134687 1102 14-15 0.037439692088476306 1102 16-17 0.04604032986843265 1102 18-19 -0.006734216491295797 1102 20-21 -0.013545817382038194 1102 22-23 -0.033031504853333615 1102 24-25 0.08832129262043509 1102 26-27 -0.0072412629286162655 1102 28-29 0.024218389981797372 1102 30-31 0.03149941224400976 1102 32-33 0.17191593624649926 1102 34-35 0.14313756673077904 1102 36-37 0.1475287382646826 1102 38-39 0.13972780379221206 1102 40-41 0.1512511657372997 1102 42-43 0.28559347234455146 1102 44-45 0.2347328935028159 1102 46-47 0.41649208576259156 1102 48-49 0.3025737495849299 1102 50-51 0.3186580056003834 1102 52-53 0.39145194772460457 1102 54-55 0.3015217160801953 1102 56-57 0.27977368084927434 1102 58-59 0.3412247841771432 1102 60-61 0.1829507564434394 1102 62-63 0.19815849660505336 1102 64-65 0.13959566813419144 1102 66-67 0.34666814545743563 1102 68-69 0.22317914214418266 1102 70-71 0.27892113659937223 1102 72-73 0.32780598464423605 1102 74-75 0.22689551088561188 1102 76-77 0.1635711821713457 1102 78-79 0.2502453606785977 1102 80-81 0.3237347020107464 1102 82-83 0.20043160817936467 1102 84-85 0.3214173911459284 1102 86-87 0.3093117141033517 1102 88-89 0.22815124653735808 1102 90-91 0.24934411950047775 1102 92-93 0.2910041860194994 1102 94-95 0.24869352755523266 1102 96-97 0.24142925960570238 1102 98-99 0.23172224738972602 1102 100-101 0.1923920129770309 1102 102-103 0.06899355720523914 1102 104-105 0.10699783287100928 1102 106-107 0.01774591547448523 1102 108-109 0.08345911266427208 1102 110-111 -0.017913617490478373 1102 112-113 0.1368805910170252 1102 114-115 0.1412380313246473 1102 116-117 0.1002134183283232 1102 118-119 0.04881957138672277 1102 120-121 0.07790277285167946 1102 122-123 0.10208970077092516 1102 124-125 0.09336426302639467 1103 1 0.04269963816186362 1103 2 0.006933698562789914 1103 3 -0.023258550550863788 1103 4 0.05318347977136284 1103 5 -0.12566055618801641 1103 6 -1.0791232062246081E-4 1103 7 0.014241674412147631 1103 8 0.008596239417450136 1103 9 0.04244678995605966 1103 10-11 0.026271994598964454 1103 12-13 0.009573969538024585 1103 14-15 0.025395880218312072 1103 16-17 -0.06128539476387118 1103 18-19 0.01143910160484296 1103 20-21 -0.0057689135989633655 1103 22-23 0.0495387321641374 1103 24-25 0.012310962992508223 1103 26-27 0.07634561132003626 1103 28-29 0.05684725060601892 1103 30-31 -0.06479913774930424 1103 32-33 -0.10662040296898567 1103 34-35 -0.020392613295641127 1103 36-37 -0.0014716921364765767 1103 38-39 -0.013116554242188272 1103 40-41 0.05243699515002476 1103 42-43 0.03618341876703113 1103 44-45 -0.18137598315015424 1103 46-47 -0.21739235894569475 1103 48-49 -0.22067533395742345 1103 50-51 -0.17680794076272832 1103 52-53 -0.22965403485157054 1103 54-55 -0.11343695056668679 1103 56-57 -0.14553687585237185 1103 58-59 -0.23239607692200792 1103 60-61 -0.153556167078726 1103 62-63 -0.43613408713765267 1103 64-65 -0.23735412334387007 1103 66-67 -0.14533975017770473 1103 68-69 -0.07731507712295382 1103 70-71 -0.09767246081229075 1103 72-73 -0.2054758984350613 1103 74-75 -0.2702475158734714 1103 76-77 -0.05090959076977697 1103 78-79 -0.1696035536715712 1103 80-81 -0.24229477304825053 1103 82-83 -0.18876235433121025 1103 84-85 -0.15522963537568302 1103 86-87 -0.10177334207141797 1103 88-89 -0.08696919158133909 1103 90-91 -0.023470852523445274 1103 92-93 -0.11173257172401208 1103 94-95 -0.10311489940271912 1103 96-97 0.032877633728197964 1103 98-99 -0.07685312475886974 1103 100-101 0.08425001027588763 1103 102-103 -0.04374186481052078 1103 104-105 -0.08316527432856446 1103 106-107 -0.004909511290527746 1103 108-109 -0.09232265910438286 1103 110-111 -0.09636536061519507 1103 112-113 -0.15517628130224637 1103 114-115 -0.14808059910916427 1103 116-117 -0.018903814043781608 1103 118-119 0.043164990582610585 1103 120-121 0.06418930129526501 1103 122-123 0.14208732614381603 1103 124-125 0.13436368421103495 1104 1 0.03634070958660374 1104 2 0.061559749352667836 1104 3 0.12034313972660726 1104 4 -0.03510647707032888 1104 5 -0.11646768728753898 1104 6 -0.013882729418263295 1104 7 -0.027169694399269417 1104 8 0.01571059926455831 1104 9 -0.07558827321473416 1104 10-11 0.02080638496378384 1104 12-13 -0.03621332076004791 1104 14-15 -0.0017599425042931216 1104 16-17 0.06390909659695865 1104 18-19 0.01645402781079497 1104 20-21 0.16358235979633662 1104 22-23 0.15413323511396726 1104 24-25 0.19029550669699802 1104 26-27 0.1739083797181138 1104 28-29 0.11788212706510848 1104 30-31 0.009082362554373447 1104 32-33 -0.008721231582661915 1104 34-35 0.017110686350896742 1104 36-37 -0.05281526528347058 1104 38-39 -0.0746074794094298 1104 40-41 0.03470313873776831 1104 42-43 -0.2073063517921767 1104 44-45 -0.2226285397055605 1104 46-47 -0.13494626390299658 1104 48-49 -0.034737837310721886 1104 50-51 -0.2695306910476276 1104 52-53 -0.2602731451166349 1104 54-55 -0.23918996746302668 1104 56-57 -0.30359477365024645 1104 58-59 -0.348952469222624 1104 60-61 -0.38468433500766963 1104 62-63 -0.3077429625966559 1104 64-65 -0.32250270937826286 1104 66-67 -0.22391050425692782 1104 68-69 -0.17005177713303254 1104 70-71 -0.21788099847332631 1104 72-73 -0.14281856163074735 1104 74-75 -0.16898163196523086 1104 76-77 -0.10842055203460887 1104 78-79 -0.10549490891956026 1104 80-81 -0.10878356283448198 1104 82-83 -0.12233429950235575 1104 84-85 -0.2470435074116324 1104 86-87 -0.1412821829780242 1104 88-89 -0.14778516466051883 1104 90-91 -0.14945672252488862 1104 92-93 -0.1438083552156897 1104 94-95 -0.0784882691627189 1104 96-97 -0.06912275686410396 1104 98-99 -0.07949284198720363 1104 100-101 -0.08418165431262281 1104 102-103 -0.04816017200063527 1104 104-105 -0.03198158649336591 1104 106-107 -0.09062788994615545 1104 108-109 -0.038787369518814785 1104 110-111 -0.07077244364181468 1104 112-113 -0.08605753909531444 1104 114-115 -0.0893447446670308 1104 116-117 -0.17953329002772023 1104 118-119 -0.11245277836272294 1104 120-121 -0.10061167904850521 1104 122-123 -0.040025799689139774 1104 124-125 0.09360312373253521 1105 1 0.08500211020113824 1105 2 0.11153280885683614 1105 3 0.11957445144790313 1105 4 0.06191155329857878 1105 5 0.06379751536947964 1105 6 0.043510928435360086 1105 7 -0.005598574610800711 1105 8 0.06810622304625724 1105 9 -0.014764122880528419 1105 10-11 0.05940670658545599 1105 12-13 0.09411002760032261 1105 14-15 0.12367146948974295 1105 16-17 0.09048449149885585 1105 18-19 0.11759998704847874 1105 20-21 0.11968651445766909 1105 22-23 0.13154430547338336 1105 24-25 0.04261166121686699 1105 26-27 0.10351307214918393 1105 28-29 0.146675692135247 1105 30-31 0.08419485732749621 1105 32-33 0.17979150742322503 1105 34-35 0.2850937273607812 1105 36-37 0.2558229602636004 1105 38-39 0.21171018128178787 1105 40-41 0.27208167246747195 1105 42-43 0.108189218108528 1105 44-45 0.10619945511022166 1105 46-47 0.22272470422816326 1105 48-49 0.13770578008014667 1105 50-51 0.14332044555005297 1105 52-53 0.13396564545274003 1105 54-55 0.16097157149459207 1105 56-57 0.29506777115523164 1105 58-59 0.18578724814141623 1105 60-61 0.23267480988643285 1105 62-63 0.22546873977223214 1105 64-65 0.2993104588032409 1105 66-67 0.1926150455268143 1105 68-69 0.2655510762711444 1105 70-71 0.2726081743598314 1105 72-73 0.22732148734061752 1105 74-75 0.24488211361663303 1105 76-77 0.29218098034911577 1105 78-79 0.2822069107601024 1105 80-81 0.10231290494406409 1105 82-83 0.19519376958182022 1105 84-85 0.12844459404273323 1105 86-87 0.07800409151546006 1105 88-89 0.22195337730737208 1105 90-91 0.26818285316750234 1105 92-93 0.17993943181500782 1105 94-95 0.15269655096005863 1105 96-97 0.15353472021566716 1105 98-99 0.06267496297608943 1105 100-101 0.10992565831571 1105 102-103 0.19045928881498497 1105 104-105 0.25113910068691325 1105 106-107 0.1613775647241198 1105 108-109 0.04638256363962512 1105 110-111 0.11789060044698374 1105 112-113 0.18048590167408207 1105 114-115 0.17040636259774544 1105 116-117 0.19876900105861495 1105 118-119 0.2514789945715421 1105 120-121 0.13542659101801036 1105 122-123 0.25144280059087265 1105 124-125 -0.046736576156583 1106 1 0.10992286933421269 1106 2 0.11972364698275584 1106 3 0.07452838494327807 1106 4 0.08994221908383082 1106 5 0.15279227246370652 1106 6 0.11030407003836018 1106 7 0.13034949642707971 1106 8 0.02298726810447249 1106 9 0.07315148040795805 1106 10-11 0.09899525135768528 1106 12-13 0.08541301977190585 1106 14-15 0.013922742253463127 1106 16-17 0.04492415657649218 1106 18-19 0.021709680097046657 1106 20-21 0.13014954837812098 1106 22-23 -0.02323935253406262 1106 24-25 0.06565959245067177 1106 26-27 0.04928306256233128 1106 28-29 0.04632603010475833 1106 30-31 0.13364510641360994 1106 32-33 0.043820152579023386 1106 34-35 0.007994160743280077 1106 36-37 0.012188297357440092 1106 38-39 0.027726605476289023 1106 40-41 0.06290704290777427 1106 42-43 0.09514016317797314 1106 44-45 0.00650578901417731 1106 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-0.40755503949340266 2216 96-97 -0.41778456146307263 2216 98-99 -0.4557442046901343 2216 100-101 -0.4527642742516065 2216 102-103 -0.4975888931303487 2216 104-105 -0.5018457809986074 2216 106-107 -0.46616726677893894 2216 108-109 -0.47904858593716426 2216 110-111 -0.5198318617148416 2216 112-113 -0.46543910436561475 2216 114-115 -0.5545127902341278 2216 116-117 -0.72139256612612 2216 118-119 -0.6206112490931304 2216 120-121 -0.7250008923569311 2216 122-123 -0.7168032291032453 2216 124-125 -0.7174501928117323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64.0 3 3.0 4 9.0 5 29.0 6 54.0 7 110.0 8 203.0 9 402.0 10 739.0 11 1237.0 12 1564.0 13 2519.0 14 4256.0 15 4732.0 16 1823.0 17 1170.0 18 1042.0 19 937.0 20 936.0 21 939.0 22 1039.0 23 1112.0 24 1340.0 25 1833.0 26 2305.0 27 3069.0 28 3867.0 29 5046.0 30 6344.0 31 8159.0 32 10748.0 33 14153.0 34 20615.0 35 34290.0 36 73701.0 37 239061.0 38 218063.0 39 2803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.632398493593 20.036138250443148 14.994837392793837 23.336625863170017 2 13.474241999295856 20.521813592395226 44.07533163463203 21.928612773676893 3 19.638349673885152 25.625376688009833 29.133871189110806 25.602402448994205 4 11.56156200955967 17.658089617434168 39.43766820424994 31.342680168756225 5 12.056795846799968 38.26192282647008 34.796492003656155 14.8847893230738 6 29.12208570286253 37.52901616551 19.567935123135953 13.780963008491517 7 27.461674792187562 32.330870813168715 22.13806622548171 18.069388169162007 8 24.170988011624367 38.183931160825644 20.308928923075086 17.336151904474907 9 25.45321907876285 18.265116750905545 22.18073266936788 34.100931500963725 10-11 23.9237165754659 26.76327284444948 29.51436337488588 19.79864720519874 12-13 26.390314418871096 24.268703119125906 29.127680079246204 20.21330238275679 14-15 20.925130833815693 28.46799121608316 27.063951927150775 23.54292602295037 16-17 19.828260104189667 30.105278704372267 29.924468459651866 20.1419927317862 18-19 20.51935206678641 28.099060741501024 31.82558673819512 19.556000453517445 20-21 21.423882605510773 27.620705757384524 30.38631213196735 20.569099505137352 22-23 20.194475876622093 29.153852408086056 30.643451693197743 20.008220022094108 24-25 20.981298372707798 27.683883422147165 31.933297131502158 19.40152107364288 26-27 19.566144922693177 28.85236216948424 31.60598881721457 19.975504090608013 28-29 19.304701066362732 29.413962966720174 30.076038763806924 21.205297203110174 30-31 20.2763474811488 27.793628522688195 31.186583034406933 20.743440961756068 32-33 19.571067973910814 28.641491475662225 31.927628163433365 19.859812386993596 34-35 20.061732078601736 28.807457975044603 31.553550862578245 19.577259083775413 36-37 19.543096091992435 28.335889341743297 31.44755607802887 20.6734584882354 38-39 20.318327475399663 29.16728826404263 30.56192900065044 19.952455259907268 40-41 21.828137773826075 27.379250980134746 31.386017937808436 19.406593308230747 42-43 19.708092899468312 29.192276478556384 31.218708788093974 19.880921833881334 44-45 19.747701084264733 29.148118200967904 30.35247554884562 20.751705165921745 46-47 21.259152399763693 28.141652593702077 30.06000155150705 20.53919345502718 48-49 20.85374659115999 28.634032307150658 29.723145501524655 20.7890756001647 50-51 19.696829555015842 29.867927962334186 29.946771373501452 20.48847110914852 52-53 19.949695367557986 29.230243646280265 30.1140059315308 20.70605505463095 54-55 20.02794204524433 28.40876541810131 30.436316602915642 21.126975933738716 56-57 20.698446702749152 28.69974758173757 28.87376998311244 21.728035732400837 58-59 20.981298372707798 29.277087224532906 28.447478502676347 21.294135900082946 60-61 20.284686625412494 27.86476825855268 31.07765591154023 20.772889204494597 62-63 20.61050310599777 27.820162430853507 30.737144868987166 20.83218959416156 64-65 20.86963462008963 28.993638821093338 29.17899915860579 20.957727400211244 66-67 20.817867990619348 29.773867847403313 28.52647109721385 20.881793064763485 68-69 21.389240298605433 28.05221716324838 30.28840129133125 20.270141246814934 70-71 22.162830664940117 27.92876792438193 29.527416919781118 20.380984490896832 72-73 21.080505313911647 28.961489804808476 29.502354113582253 20.455650767697623 74-75 20.413655648977496 29.653178500886153 30.046500456501114 19.88666539363524 76-77 20.799145477655316 28.16761050012233 30.512474713418747 20.52076930880361 78-79 20.51331014029204 28.180440269962226 30.10072861158021 21.20552097816552 80-81 20.967722686016742 28.82961170552396 29.803704521449585 20.39896108700971 82-83 20.618111457879568 28.64693666867567 30.73617517708066 19.998776696364104 84-85 20.474149505606313 28.65111380304215 30.510013187809932 20.36472350354161 86-87 19.525343270934904 30.06865418698047 29.02720507939539 21.37879746268924 88-89 20.314299524403413 28.79671677238795 29.681374157859874 21.20760954534876 90-91 20.729590523752158 28.652670602671158 30.27789484676809 20.339844026808592 92-93 20.199950471121085 29.643481581821113 30.188448433276243 19.96811951378156 94-95 20.055392198546645 29.7314231752127 29.767749596828935 20.445435029411723 96-97 20.85859505069251 29.00318655678814 29.820786017341078 20.317432375178274 98-99 20.955405175577578 28.54213473926678 30.37108646550361 20.131373619652038 100-101 20.289490761806018 28.56530138815333 30.46962995009809 20.675577899942567 102-103 20.137503235436064 29.198887382955537 29.858282306062183 20.80532707554622 104-105 20.739833462504446 28.636229028963918 29.71142004432347 20.912517464208165 106-107 20.447087486190295 28.244673986586033 29.95853172930671 21.349706797916966 108-109 19.996807456826073 29.00283748463402 29.582194560145126 21.418160498394776 110-111 19.69751221513558 29.44567528253329 29.767110514341127 21.089701987990004 112-113 20.487128033360438 28.206652691885925 29.380459675795034 21.925759598958606 114-115 20.152429631652012 28.757684328780737 28.525019152543763 22.56486688702349 116-117 20.658614071429053 28.7115574111053 27.963759591765797 22.666068925699854 118-119 20.488933942300307 27.95944596238637 29.52423149994294 22.02738859537039 120-121 19.989154288084187 28.204818360432693 29.81407137880775 21.99195597267537 122-123 20.69915933791334 28.505904028763023 28.98098626744542 21.81395036587822 124-125 21.142064751265657 28.820303041831053 28.293004586728248 21.744627620175038 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40.0 1 35.0 2 66.0 3 92.5 4 52.5 5 33.5 6 81.5 7 229.5 8 376.5 9 384.0 10 298.0 11 233.0 12 232.0 13 269.5 14 321.5 15 388.0 16 462.5 17 568.5 18 710.0 19 876.5 20 1056.0 21 1264.0 22 1645.5 23 2134.0 24 2634.0 25 3315.0 26 4284.5 27 5427.0 28 6603.0 29 8023.0 30 9883.0 31 12357.0 32 15848.5 33 20295.5 34 25831.0 35 31341.5 36 35344.0 37 36547.5 38 34222.0 39 29952.5 40 26607.0 41 24860.5 42 23280.5 43 22045.5 44 21350.5 45 21135.0 46 21071.0 47 22233.0 48 25236.5 49 23975.0 50 19828.5 51 17760.5 52 16035.0 53 14548.5 54 13321.0 55 11859.5 56 10122.0 57 8685.5 58 7440.0 59 6179.5 60 4860.5 61 3767.0 62 2959.5 63 2140.0 64 1480.5 65 1072.0 66 788.5 67 583.5 68 417.5 69 265.0 70 190.5 71 138.5 72 105.5 73 82.0 74 54.5 75 39.0 76 22.0 77 10.5 78 7.0 79 3.5 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.5 98 4.5 99 4.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0167085374659116 2 0.0 3 0.0 4 0.0 5 0.11337936137582871 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004326317736709253 22-23 2.2377505534703038E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 7.459168511567679E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0012680586469665054 92-93 0.0 94-95 7.459168511567678E-4 96-97 0.0 98-99 0.00208856718323895 100-101 1.4918337023135358E-4 102-103 6.713251660410911E-4 104-105 0.002461525608817334 106-107 0.006638659975295233 108-109 5.967334809254143E-4 110-111 0.006116518179485497 112-113 0.009100185584112568 114-115 0.005892743124138467 116-117 0.0021631588683546266 118-119 7.459168511567679E-5 120-121 7.459168511567678E-4 122-123 0.0016410170725448892 124-125 0.0018647921278919196 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 670316.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.31920771252686 #Duplication Level Percentage of deduplicated Percentage of total 1 90.30606807963024 72.53311839789413 2 7.190596887886976 11.550860900304864 3 1.268261150219326 3.055971922745828 4 0.43864541012959113 1.4092660723338066 5 0.22665404997865 0.9102336859560316 6 0.13474177134440157 0.6493411392098873 7 0.07890469868263239 0.4436294018089286 8 0.05061791897584585 0.32524729185574547 9 0.04218384597251443 0.30493557781016634 >10 0.2265746006077619 3.3593261829678487 >50 0.02004647691601988 1.1446697245817468 >100 0.013920924715148272 2.1821183927847887 >500 0.0018561232939710549 1.0021315727556361 >1k 9.280616469855274E-4 1.1291497369906558 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2579 0.38474391182666084 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1546 0.23063749037767262 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1190 0.17752821057531074 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1135 0.1693231252125863 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1124 0.16768210814004142 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 928 0.1384421675746961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 775 0.11561711192929901 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 772 0.11516956181860495 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 728 0.10860549352842541 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 700 0.1044283591619475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.459168511567679E-5 0.0 22-23 7.459168511567679E-5 0.0 0.0 2.2377505534703035E-4 0.0 24-25 2.2377505534703035E-4 0.0 0.0 3.729584255783839E-4 0.0 26-27 2.9836674046270715E-4 0.0 0.0 5.221417958097375E-4 0.0 28-29 2.9836674046270715E-4 0.0 0.0 0.001044283591619475 0.0 30-31 2.9836674046270715E-4 0.0 0.0 0.0024615256088173342 0.0 32-33 2.9836674046270715E-4 0.0 0.0 0.005370601328328729 0.0 34-35 2.9836674046270715E-4 0.0 0.0 0.010069877490616367 0.0 36-37 2.9836674046270715E-4 0.0 0.0 0.01790200442776243 0.0 38-39 2.9836674046270715E-4 0.0 0.0 0.030284224156964773 0.0 40-41 2.9836674046270715E-4 0.0 0.0 0.04676898656752934 0.0 42-43 2.9836674046270715E-4 0.0 0.0 0.06638659975295233 0.0 44-45 3.729584255783839E-4 0.0 0.0 0.08622798799372236 0.0 46-47 4.475501106940607E-4 0.0 0.0 0.10912763532423514 0.0 48-49 4.475501106940607E-4 0.0 0.0 0.13620441702122582 0.0 50-51 4.475501106940607E-4 0.0 0.0 0.1683534333060825 0.0 52-53 4.475501106940607E-4 0.0 0.0 0.1979663322970062 0.0 54-55 4.475501106940607E-4 0.0 0.0 0.23294983261625862 0.0 56-57 4.475501106940607E-4 0.0 0.0 0.27419903448522787 0.0 58-59 4.475501106940607E-4 0.0 0.0 0.31828272038859284 0.0 60-61 4.475501106940607E-4 0.0 0.0 0.3692288413226001 0.0 62-63 4.475501106940607E-4 0.0 0.0 0.4237553631421598 0.0 64-65 4.475501106940607E-4 0.0 0.0 0.47887861844264495 0.0 66-67 4.475501106940607E-4 0.0 0.0 0.5482488856002243 0.0 68-69 4.475501106940607E-4 0.0 0.0 0.6316423895595511 0.0 70-71 4.475501106940607E-4 0.0 0.0 0.7238377123625275 0.0 72-73 4.475501106940607E-4 0.0 0.0 0.8135715095566867 0.0 74-75 4.475501106940607E-4 0.0 0.0 0.9042749986573497 0.0 76-77 4.475501106940607E-4 0.0 0.0 1.0086287661341815 0.0 78-79 4.475501106940607E-4 0.0 0.0 1.1334206553327086 0.0 80-81 4.475501106940607E-4 0.0 0.0 1.2603757033995906 0.0 82-83 4.475501106940607E-4 0.0 0.0 1.4003097046766002 0.0 84-85 4.475501106940607E-4 0.0 0.0 1.5350968796806281 0.0 86-87 4.475501106940607E-4 0.0 0.0 1.6768956730855298 7.459168511567679E-5 88-89 4.475501106940607E-4 0.0 0.0 1.8340603536242608 1.4918337023135358E-4 90-91 5.967334809254143E-4 0.0 0.0 2.0190477327111394 1.4918337023135358E-4 92-93 5.967334809254143E-4 0.0 0.0 2.2029908282063984 1.4918337023135358E-4 94-95 5.967334809254143E-4 0.0 0.0 2.3902159578467472 1.4918337023135358E-4 96-97 5.967334809254143E-4 0.0 0.0 2.576471395580592 1.4918337023135358E-4 98-99 5.967334809254143E-4 0.0 0.0 2.792712690730939 1.4918337023135358E-4 100-101 5.967334809254143E-4 0.0 0.0 3.0254387482918506 1.4918337023135358E-4 102-103 5.967334809254143E-4 0.0 0.0 3.2768127271316816 1.4918337023135358E-4 104-105 5.967334809254143E-4 0.0 0.0 3.529454764618478 1.4918337023135358E-4 106-107 5.967334809254143E-4 0.0 0.0 3.786497711527101 1.4918337023135358E-4 108-109 5.967334809254143E-4 0.0 0.0 4.044584942027342 1.4918337023135358E-4 110-111 5.967334809254143E-4 0.0 0.0 4.335567105663598 1.4918337023135358E-4 112-113 5.967334809254143E-4 0.0 0.0 4.673392847552497 1.4918337023135358E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 695 0.0 42.00557 2 GTATACG 65 0.0019242718 36.669685 1 GTACCTG 835 0.0 34.967945 1 TACATGG 2900 0.0 30.61145 2 GTACATG 3020 0.0 30.18874 1 TAGGACC 1010 0.0 30.084614 4 ACATGGG 2865 0.0 29.321764 3 ACCTGGG 940 0.0 28.52202 3 GTAGCCC 340 0.0 28.037333 3 GTGTAGC 450 0.0 27.807844 1 GTGTAGG 325 0.0 27.502262 1 TAGCCCT 630 0.0 27.425406 4 AGTACTT 2280 0.0 27.398964 12-13 AGGACCG 90 0.009464503 26.479702 5 AGAGTAC 3740 0.0 26.168205 10-11 GAGTACT 2235 0.0 25.554852 12-13 CATGGGG 1700 0.0 24.883133 4 TAAGACG 145 1.327201E-4 24.653517 4 CCTAGAC 295 1.7826096E-10 24.23566 3 GTACTTT 2400 0.0 24.047646 14-15 >>END_MODULE