FastQCFastQC Report
Fri 27 May 2016
SRR939956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310118
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT97840.746802959733398No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53050.40492535786852785No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42980.328062052425812No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34400.2625717683445308No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30640.2338720634324542No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26380.2013559083990908No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24250.18509783088240908No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23470.17914416869320168No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20770.15853533803825304No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19810.1512077538053824No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19140.14609371064285812No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16440.1254848799879095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4500.01428.0974108-109
CCGCGCC4950.01198.4034108-109
CGTCGCT1350.01123.2783110-111
CCGCGCG1200.01029.8779108-109
TTGCGAC300.0079885871010.95044110-111
CGCGTAG300.0079885871010.95044110-111
CCTCGCC5550.0801.6347108-109
CGTGTCG654.923703E-5760.5252108-109
CGTTGTG907.062443E-5673.967110-111
GGTCGCC1501.0932126E-9659.1219108-109
CGTCGCG757.561443E-5659.1219108-109
TTGTTCG3000.0659.1219108-109
CCGCTCG2057.2759576E-12602.85535108-109
CGTCGCC2057.2759576E-12602.85535108-109
CCGTTCG851.1003904E-4581.5781108-109
CGGCGCG1051.12107715E-4577.686110-111
CGGTGCG502.3775219E-4494.34143108-109
CGGTACT1302.1263337E-4466.59253110-111
CTCCGCC2707.376002E-9449.3113110-111
CATCGCC2956.366463E-11418.93338108-109
Produced by FastQC (version 0.11.5)