##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939956_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1310118 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8914914534416 34.0 31.0 34.0 30.0 34.0 2 31.96858908892176 34.0 31.0 34.0 30.0 34.0 3 32.09633178080142 34.0 31.0 34.0 30.0 34.0 4 35.26639356149599 37.0 35.0 37.0 33.0 37.0 5 35.32890319803254 37.0 35.0 37.0 33.0 37.0 6 35.38785361318599 37.0 37.0 37.0 33.0 37.0 7 35.32408683797948 37.0 37.0 37.0 33.0 37.0 8 35.28191277426919 37.0 37.0 37.0 33.0 37.0 9 36.89363324524967 39.0 39.0 39.0 34.0 39.0 10-11 36.89034155702006 39.0 39.0 39.0 34.0 39.0 12-13 36.81735423832052 39.0 38.0 39.0 34.0 39.0 14-15 38.32243965810713 41.0 39.0 41.0 34.0 41.0 16-17 38.356519031110174 41.0 39.0 41.0 34.0 41.0 18-19 38.4208243074288 41.0 39.0 41.0 34.0 41.0 20-21 38.49387917729548 41.0 39.0 41.0 34.0 41.0 22-23 38.51511123425524 41.0 39.0 41.0 34.0 41.0 24-25 38.558240173785876 41.0 39.0 41.0 34.0 41.0 26-27 38.5320486398935 41.0 39.0 41.0 34.0 41.0 28-29 38.494372262651154 41.0 39.0 41.0 34.0 41.0 30-31 38.42895563605721 41.0 39.0 41.0 33.0 41.0 32-33 38.305846114624785 40.5 38.5 41.0 33.0 41.0 34-35 38.191294982589355 40.0 38.0 41.0 33.0 41.0 36-37 38.364720582420816 41.0 39.0 41.0 33.0 41.0 38-39 38.33975832711252 41.0 39.0 41.0 33.0 41.0 40-41 38.30642468846318 41.0 39.0 41.0 33.0 41.0 42-43 38.19340891431153 41.0 38.5 41.0 33.0 41.0 44-45 38.07979662900594 41.0 38.0 41.0 33.0 41.0 46-47 37.97622504232443 41.0 38.0 41.0 33.0 41.0 48-49 37.8449403030872 40.0 38.0 41.0 33.0 41.0 50-51 37.706682527833365 40.0 38.0 41.0 32.0 41.0 52-53 37.58325242459076 40.0 37.5 41.0 32.0 41.0 54-55 37.39285430777991 40.0 37.0 41.0 32.0 41.0 56-57 37.168260797882326 40.0 36.5 41.0 31.0 41.0 58-59 36.948687828119304 40.0 36.0 41.0 31.0 41.0 60-61 36.650040301713275 39.5 35.5 41.0 31.0 41.0 62-63 36.381997652119885 39.0 35.0 41.0 31.0 41.0 64-65 36.05949273271568 39.0 35.0 41.0 30.0 41.0 66-67 35.703572502629534 38.0 35.0 40.5 30.0 41.0 68-69 35.29555467522773 37.0 35.0 40.0 30.0 41.0 70-71 34.87435979049215 37.0 35.0 39.5 29.0 41.0 72-73 34.47492172460801 36.0 34.5 39.0 29.0 41.0 74-75 34.08206283708796 36.0 34.0 39.0 29.0 40.5 76-77 33.68004370598679 35.0 34.0 37.0 29.0 39.5 78-79 33.24381429764342 35.0 34.0 37.0 28.0 39.0 80-81 32.82634312329118 35.0 34.0 36.5 27.0 39.0 82-83 32.65631340077764 35.0 34.0 36.0 28.0 37.5 84-85 32.29786057439101 35.0 34.0 36.0 27.5 37.0 86-87 31.726703243524632 35.0 34.0 35.5 14.0 37.0 88-89 16.463763187743396 18.0 16.5 18.5 2.0 19.0 90-91 2.8448517614443887 2.0 2.0 2.0 2.0 2.0 92-93 2.3138549352043096 2.0 2.0 2.0 2.0 2.0 94-95 2.1048520056971967 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11809248260830074 1101 2 0.14473348239237893 1101 3 0.20543159956048385 1101 4 0.25441095750397125 1101 5 0.18371072880837147 1101 6 0.19296882626866818 1101 7 0.15118586930677225 1101 8 0.12504011393809833 1101 9 0.21296236251555456 1101 10-11 0.14896525380064674 1101 12-13 0.17000058611694868 1101 14-15 0.3359720353367237 1101 16-17 0.26460364032116246 1101 18-19 0.22676888599569622 1101 20-21 0.21387168502222664 1101 22-23 0.22009842421314119 1101 24-25 0.10353394733675003 1101 26-27 0.23114487236621528 1101 28-29 0.19497092018531959 1101 30-31 0.25485671089039386 1101 32-33 0.3169324166245815 1101 34-35 0.21373664098213396 1101 36-37 0.2377653174993526 1101 38-39 0.2151732226582581 1101 40-41 0.19210117706864338 1101 42-43 0.2639777897888038 1101 44-45 0.26427814362411084 1101 46-47 0.22501908962236428 1101 48-49 0.33222457623958945 1101 50-51 0.2775629296245157 1101 52-53 0.25331507165515177 1101 54-55 0.22994244822855592 1101 56-57 0.24690426085944495 1101 58-59 0.13897089043433652 1101 60-61 0.19155162197262143 1101 62-63 0.24808395157877783 1101 64-65 0.3524248072562841 1101 66-67 0.3096461136172195 1101 68-69 0.3395391512059618 1101 70-71 0.39812372472394486 1101 72-73 0.353591892289316 1101 74-75 0.24925461288141548 1101 76-77 0.17364590934989366 1101 78-79 0.26642517987512804 1101 80-81 0.2296110040887953 1101 82-83 0.25397243275634196 1101 84-85 0.03934627621762132 1101 86-87 0.1363346598073214 1101 88-89 0.9744571918265734 1101 90-91 -0.0173426009960429 1101 92-93 -0.19887220549332296 1101 94-95 -0.0202430815320227 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.027338904602469682 1102 2 0.06573055555829299 1102 3 0.10810387804899335 1102 4 0.15171079265362408 1102 5 0.12248566612575473 1102 6 0.07303464301825358 1102 7 0.16216982601135044 1102 8 0.11959024275828511 1102 9 0.18663720506866355 1102 10-11 0.1195215435325494 1102 12-13 0.11497684569209099 1102 14-15 0.06520240259764165 1102 16-17 0.11498868187924671 1102 18-19 0.051549836155608375 1102 20-21 0.014782495552907449 1102 22-23 0.08320461325799755 1102 24-25 0.09420589760525644 1102 26-27 0.10645550916238733 1102 28-29 0.21525763147015908 1102 30-31 0.2687836967448547 1102 32-33 0.13440538166051397 1102 34-35 0.2126173305402972 1102 36-37 0.2383827351235297 1102 38-39 0.14243084575309695 1102 40-41 0.17120630248323465 1102 42-43 0.18593870075974195 1102 44-45 0.1760919972231818 1102 46-47 0.26393552554789323 1102 48-49 0.18533640425230402 1102 50-51 0.1995590070252149 1102 52-53 0.2301212688438099 1102 54-55 0.22417762156821652 1102 56-57 0.1880455287264695 1102 58-59 0.1021033617582674 1102 60-61 0.11912265816072676 1102 62-63 0.09360245282255875 1102 64-65 0.14282178072228646 1102 66-67 0.1896127548653439 1102 68-69 0.12932641898413522 1102 70-71 0.09957723963945142 1102 72-73 0.22979264077900297 1102 74-75 0.23383397919469218 1102 76-77 0.22566955229587649 1102 78-79 0.3399947390731839 1102 80-81 0.2651738273731752 1102 82-83 0.25998959397405486 1102 84-85 0.21413314914465076 1102 86-87 0.40765029763997873 1102 88-89 1.3857293590164197 1102 90-91 0.13439483656812 1102 92-93 -0.04374321256115854 1102 94-95 0.08943684843125599 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.03833070088923307 1103 2 0.14030768189976683 1103 3 0.19569483847674007 1103 4 0.029156955040917865 1103 5 0.07381617831575937 1103 6 0.09140632626866818 1103 7 0.1276906414742598 1103 8 0.09334753388883854 1103 9 0.14824946226925562 1103 10-11 0.17031493052478908 1103 12-13 0.13651844325980989 1103 14-15 0.1443637379229301 1103 16-17 0.27505237800588844 1103 18-19 0.2139436858725361 1103 20-21 0.1973037970911946 1103 22-23 0.15701152458260736 1103 24-25 0.14215386728749735 1103 26-27 0.057287883449959054 1103 28-29 0.05707837092423773 1103 30-31 0.021857557565269303 1103 32-33 0.06895289076251743 1103 34-35 0.07502628076046136 1103 36-37 0.09897798683432768 1103 38-39 0.01818661551539691 1103 40-41 0.0842752072410562 1103 42-43 0.20914597020752979 1103 44-45 0.13971110236795425 1103 46-47 0.031563501568172114 1103 48-49 0.03609041675682789 1103 50-51 0.019798536151611756 1103 52-53 0.08578890170441156 1103 54-55 0.12291504675073384 1103 56-57 0.10404711800230615 1103 58-59 0.11479254991709809 1103 60-61 0.08477437443567482 1103 62-63 0.06287382226842908 1103 64-65 0.14768342794593536 1103 66-67 0.1782287029029348 1103 68-69 0.07421241167394754 1103 70-71 0.1370303595761584 1103 72-73 0.07481456778192097 1103 74-75 0.08912722679767171 1103 76-77 0.03811057991639899 1103 78-79 0.10044804009680064 1103 80-81 0.031123473300624482 1103 82-83 0.1918572849730822 1103 84-85 0.12966147018313734 1103 86-87 0.04780902864968439 1103 88-89 0.6036232170728795 1103 90-91 -0.5907435400354517 1103 92-93 -0.3383811340647518 1103 94-95 -0.1137140636748799 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.10719723005319537 1104 2 0.1900455056934227 1104 3 0.23632467121787215 1104 4 0.29773395441496575 1104 5 0.20575001118576353 1104 6 0.16656149177854473 1104 7 0.1999113550630085 1104 8 0.12681254024044364 1104 9 0.1751997609786784 1104 10-11 0.24125640602969156 1104 12-13 0.2539937502539402 1104 14-15 0.1547401796907053 1104 16-17 0.2046673868889215 1104 18-19 0.25331729358283184 1104 20-21 0.17121159759564364 1104 22-23 0.25387835494878885 1104 24-25 0.27961730529953144 1104 26-27 0.3044275047931819 1104 28-29 0.34727742807945106 1104 30-31 0.338811590142555 1104 32-33 0.3356502990324799 1104 34-35 0.32320206481517744 1104 36-37 0.29036949409724144 1104 38-39 0.3181107012067983 1104 40-41 0.4073046858113045 1104 42-43 0.32994651952910203 1104 44-45 0.23639497896275685 1104 46-47 0.2075851660562762 1104 48-49 0.18735233751828417 1104 50-51 0.15261909072016522 1104 52-53 0.1845023857425545 1104 54-55 0.16236560435699943 1104 56-57 0.17102689643262892 1104 58-59 0.21021042093353515 1104 60-61 0.24216423971726186 1104 62-63 0.26132098775069323 1104 64-65 0.1331659311217379 1104 66-67 0.21218158597821457 1104 68-69 0.2200937368115632 1104 70-71 0.09264594723747877 1104 72-73 0.1427731201228113 1104 74-75 0.09597519221365758 1104 76-77 0.16219753703847317 1104 78-79 0.09826381370358916 1104 80-81 0.04511569719001329 1104 82-83 0.058883981265225316 1104 84-85 0.12333690789140661 1104 86-87 0.039167771078389535 1104 88-89 0.5549288914013601 1104 90-91 -0.6101024078852966 1104 92-93 -0.3383811340647518 1104 94-95 -0.1137140636748799 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.0902579719455936 1105 2 -0.1037151609104967 1105 3 0.07332716359733382 1105 4 0.005480598503901035 1105 5 0.019657805084278834 1105 6 -0.026354009144746726 1105 7 -0.01607829893852397 1105 8 -0.19989656656483135 1105 9 0.05149561366702926 1105 10-11 -0.04598262223939287 1105 12-13 0.02398269182975099 1105 14-15 -0.10617463490508783 1105 16-17 -0.032377279094191636 1105 18-19 0.006257576704037149 1105 20-21 0.17141483049805117 1105 22-23 -0.1654498436997116 1105 24-25 0.022852662049622552 1105 26-27 0.04151284512083464 1105 28-29 -0.011185986805251957 1105 30-31 -0.014501730857986672 1105 32-33 -0.04286306894483971 1105 34-35 0.05740147016459218 1105 36-37 -0.172276742665197 1105 38-39 -0.0811901720561039 1105 40-41 -0.020224737842951868 1105 42-43 0.04038030794310288 1105 44-45 -0.039911535123749786 1105 46-47 0.08214729216118144 1105 48-49 -0.002834372993383738 1105 50-51 -0.01278583834405822 1105 52-53 -0.047209360036404746 1105 54-55 -0.09466196451937492 1105 56-57 -0.023561111326863227 1105 58-59 -0.0903836743732569 1105 60-61 -0.13055976694722204 1105 62-63 -0.08339477632033265 1105 64-65 -0.0541095791191708 1105 66-67 -0.11621682491817609 1105 68-69 -0.10605759007439985 1105 70-71 -0.304982558009506 1105 72-73 -0.11488025061426299 1105 74-75 -0.1438464348211994 1105 76-77 -0.22923222586479852 1105 78-79 -0.12992425669162344 1105 80-81 -0.17444080530500372 1105 82-83 -0.17209465457001727 1105 84-85 -0.11836323528783765 1105 86-87 -0.15628078364970222 1105 88-89 1.1604370305736929 1105 90-91 -0.8412823331389001 1105 92-93 -0.3383811340647518 1105 94-95 -0.1137140636748799 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.10032156352547261 1106 2 0.18397355214542088 1106 3 0.21595659136495726 1106 4 0.1124786571105858 1106 5 0.15309047538445952 1106 6 0.21029496726816177 1106 7 0.05183758444846376 1106 8 0.10199844651258871 1106 9 0.204228719421657 1106 10-11 0.08160584692915052 1106 12-13 0.1483905748253349 1106 14-15 0.20667237158098573 1106 16-17 0.14685355153628876 1106 18-19 0.13264994499438387 1106 20-21 0.1109606224266173 1106 22-23 0.11394571764033401 1106 24-25 0.06473979393469875 1106 26-27 -0.014832774333719101 1106 28-29 0.1568173269020079 1106 30-31 0.18327774584886924 1106 32-33 0.21709296927629396 1106 34-35 0.09984826747554365 1106 36-37 0.14815790031970977 1106 38-39 0.18338055355720684 1106 40-41 0.17758271550566462 1106 42-43 -0.0752605441060723 1106 44-45 0.1266275792710161 1106 46-47 0.047410777764341105 1106 48-49 0.0933068923410687 1106 50-51 0.042180705039754685 1106 52-53 -0.030073733254880608 1106 54-55 -0.0054695135984985654 1106 56-57 -0.14285337919300645 1106 58-59 0.004175385908581575 1106 60-61 0.02638813259038386 1106 62-63 0.028081003465572962 1106 64-65 0.07435398879631805 1106 66-67 0.0812596819952347 1106 68-69 0.15209136923430577 1106 70-71 0.14906322238366698 1106 72-73 0.2747146396576312 1106 74-75 0.07020696158288331 1106 76-77 0.20601895266780446 1106 78-79 0.313032829261374 1106 80-81 0.2731602705891305 1106 82-83 0.35966798258513677 1106 84-85 0.35384720845882356 1106 86-87 0.4807834138595517 1106 88-89 0.8346587164222612 1106 90-91 -0.21787590784568245 1106 92-93 -0.2935060703217638 1106 94-95 -0.1137140636748799 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.011460503232044061 1107 2 -0.006219108150880004 1107 3 0.0759515945303022 1107 4 0.04752904100497801 1107 5 0.11934798987476825 1107 6 0.1382184490050875 1107 7 0.04367360824037547 1107 8 2.523247026857689E-4 1107 9 0.08886622317954362 1107 10-11 0.13120635666785319 1107 12-13 0.07923725613706978 1107 14-15 0.1513447403166026 1107 16-17 0.16282892955657502 1107 18-19 0.1206359948991178 1107 20-21 0.060144635223437604 1107 22-23 0.12842730374030964 1107 24-25 0.09994208365466051 1107 26-27 0.09376591109860755 1107 28-29 0.20022117169437337 1107 30-31 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-0.06158792059325435 2216 7 -0.0672181018528164 2216 8 -0.09892179954068325 2216 9 -0.22932366931349435 2216 10-11 -0.2269189642955851 2216 12-13 -0.16779641029668113 2216 14-15 -0.19154986581390432 2216 16-17 -0.23508301912295337 2216 18-19 -0.2031182460276142 2216 20-21 -0.27398461727902657 2216 22-23 -0.2531912780432819 2216 24-25 -0.30634585317131524 2216 26-27 -0.2590276283809487 2216 28-29 -0.26784475524803497 2216 30-31 -0.28982794055909267 2216 32-33 -0.37285249446328805 2216 34-35 -0.38484445398199796 2216 36-37 -0.36045247053113627 2216 38-39 -0.32536071712058146 2216 40-41 -0.3752677429104381 2216 42-43 -0.4040084717921886 2216 44-45 -0.39662970343207604 2216 46-47 -0.3865462589281492 2216 48-49 -0.39467624750518127 2216 50-51 -0.42508879071320393 2216 52-53 -0.4224451644476588 2216 54-55 -0.42609461094657775 2216 56-57 -0.4675140744663153 2216 58-59 -0.3671238402142052 2216 60-61 -0.31029059634776246 2216 62-63 -0.3694598507224782 2216 64-65 -0.38388533918723056 2216 66-67 -0.39726815902175616 2216 68-69 -0.3914687220008446 2216 70-71 -0.2890584984529809 2216 72-73 -0.2658223803496611 2216 74-75 -0.40781562957225503 2216 76-77 -0.4105593450583598 2216 78-79 -0.4181004197214122 2216 80-81 -0.3647596063415648 2216 82-83 -0.31748322325083933 2216 84-85 -0.2432455778618774 2216 86-87 -0.404561886441936 2216 88-89 -1.2335460676623686 2216 90-91 -0.8412823331389001 2216 92-93 -0.3383811340647518 2216 94-95 -0.1137140636748799 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11775.0 3 2465.0 4 1718.0 5 1685.0 6 2029.0 7 2088.0 8 2205.0 9 2373.0 10 3258.0 11 3908.0 12 4652.0 13 5203.0 14 5688.0 15 6018.0 16 6556.0 17 7594.0 18 9892.0 19 12022.0 20 12333.0 21 17645.0 22 20101.0 23 25901.0 24 40736.0 25 71053.0 26 148036.0 27 419533.0 28 446251.0 29 16814.0 30 586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.363269568084704 19.677006193335256 14.334968300565293 22.624755938014744 2 14.586943081115326 21.456824236132842 39.78085809588508 24.175374586866752 3 19.665784445934325 26.289999648740736 29.076783747997442 24.967432157327497 4 12.222069492172585 17.231996945399008 38.74066437571592 31.805269186712486 5 12.865200544335718 39.549181892742645 34.27222589626769 13.31339166665394 6 30.207813342004307 37.43647518773118 18.092034457964857 14.263677012299656 7 27.375396719990107 33.595447127663306 21.772160980919274 17.256995171427306 8 25.4238167859689 34.57108443666906 20.66218462764423 19.34291414971781 9 25.68989968842501 18.293619353371223 21.095809690424833 34.920671267778935 10-11 24.377190652402238 27.508525988617766 27.40288616545761 20.711397193522384 12-13 25.84811565263754 25.04214387424428 27.169965853634338 21.939774619483845 14-15 22.524803109338244 26.52318340790677 26.06852970495787 24.883483777797114 16-17 21.75734820428115 29.62565910194212 26.706337454088036 21.9106552396887 18-19 21.814153465658297 29.3867518553929 27.500844444698892 21.298250234249913 20-21 23.23859967300056 27.677899448436527 26.917152509667247 22.166348368895665 22-23 22.699785476274347 27.770956899470008 26.958087155177253 22.571170469078396 24-25 22.935224155381423 27.82547068279346 27.07309570588298 22.166209455942138 26-27 22.384701225385804 27.595949372499273 27.739447897059655 22.279901505055268 28-29 21.793727826187844 29.052808021347925 27.047623700103195 22.105840452361033 30-31 22.218800138613467 28.0969729444218 26.726333047862866 22.957893869101866 32-33 22.24768672680191 27.917930085623073 26.92796937644192 22.9064138111331 34-35 23.21502583357059 27.841673682579493 26.76837259573 22.174927888119917 36-37 22.86043045757346 27.143634063356913 27.433951534881412 22.561983944188214 38-39 22.138845508572512 28.175439158915456 27.098207947681047 22.587507384830985 40-41 22.523772667805495 28.050183265934827 27.080843099629192 22.34520096663049 42-43 22.09010944052368 27.98797512895785 26.453227877183583 23.46868755333489 44-45 22.393763305109392 27.508382839089947 26.84847994492095 23.249373910879715 46-47 23.190239352485804 28.21081765153978 26.42937506392554 22.16956793204887 48-49 22.415678300364892 28.07624360123699 26.792197487780665 22.715880610617454 50-51 21.80387568142717 28.10510198318014 27.676361976554787 22.414660358837907 52-53 22.649104889788553 28.41625716156865 26.525167961969835 22.409469986672956 54-55 22.102227232862198 27.935632443605492 26.984637062333363 22.977503261198944 56-57 22.468930279562606 27.75295813048901 26.767207228661842 23.010904361286542 58-59 23.059411442328095 27.399287697749365 26.735492528153955 22.80580833176859 60-61 22.249637055593467 28.07678392328019 27.461839315237253 22.211739705889087 62-63 21.76857351780527 28.053618071043985 27.572287381747294 22.60552102940346 64-65 21.727372440782005 28.5997357487919 26.920191097436337 22.752700712989757 66-67 22.058776385027915 27.912294923052734 26.41178122886641 23.617147463052945 68-69 22.06617976228777 27.759961255265285 27.064105514522215 23.109753467924726 70-71 22.71070239474612 27.582973442086896 26.647714175364356 23.058609987802626 72-73 22.245858769973392 27.29238892985212 27.385395819307877 23.076356480866608 74-75 21.7357520467622 28.860186639676733 26.671795975629674 22.73226533793139 76-77 22.201549784065254 27.566219226054443 26.815408993693694 23.416821996186606 78-79 22.428535826448755 27.776007364241828 26.929401972117706 22.86605483719171 80-81 22.76684237603025 27.744638269224602 26.579476047195747 22.909043307549396 82-83 22.386673811094013 27.550965108326615 27.375865574851616 22.686495505727756 84-85 22.139358545839798 28.04073987285105 27.144407713204238 22.67549386810491 86-87 22.112701696696714 28.27499638387625 26.867665899933936 22.7446360194931 88-89 31.879459733157084 18.035180980126015 32.92050736179525 17.16485192492165 90-91 31.62099109673119 14.834054744442444 16.380431025024787 37.16452313380158 92-93 23.396647858979424 17.45170541357094 33.861508425102315 25.290138302347316 94-95 16.983531154809356 7.159580290457579 28.583107133182594 47.27378142155047 96-97 29.492983016179853 7.440470912326128 15.990683431760274 47.07586263973374 98-99 57.85928595327815 14.201949394722263 18.77869378486449 9.160070867135092 100-101 32.323069063064715 7.114135868074062 12.466531267443951 48.09626380141728 102-103 25.11886734079104 2.167212153778516 36.84399068601111 35.86992981941934 104-105 44.694889218119975 4.529842338073149 38.66045744250124 12.114811001305634 106-107 6.777749156598474 2.164176624067273 7.926520200089741 83.13155401924452 108-109 20.27165901988745 10.671831973218438 20.94119805121777 48.11531095567634 110-111 41.95335319006478 2.7094971891921036 24.915663224652103 30.421486396091012 112-113 29.220378551504567 2.9118918351573906 38.83643597988607 29.03129363345197 114-115 30.48561930914872 4.131810955340367 23.999132822662233 41.38343691284867 116-117 21.43984761160052 4.481410047314506 21.305280289865287 52.77346205121969 118-119 22.376915530597746 4.607557254490715 16.767497716586043 56.248029498325494 120-121 27.519489916594047 4.484625918087888 30.38092866923939 37.61495549607868 122-123 35.85801828116578 1.7520899421976284 34.61851541001433 27.771376366622253 124-125 40.17924290846408 1.9747229362276613 19.514137307695243 38.33189684761302 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 51.0 1 118.0 2 258.0 3 400.5 4 527.0 5 581.5 6 561.0 7 500.5 8 446.0 9 410.5 10 361.5 11 334.5 12 323.0 13 336.5 14 373.0 15 387.0 16 431.5 17 507.5 18 621.0 19 804.5 20 1162.0 21 1714.5 22 2412.0 23 3446.5 24 4826.0 25 6489.0 26 8409.5 27 10535.0 28 13005.5 29 15578.0 30 18273.0 31 21419.5 32 24533.0 33 27628.0 34 31109.0 35 34502.0 36 37419.5 37 39853.0 38 42036.5 39 43852.5 40 45124.0 41 46243.0 42 48435.0 43 52626.5 44 56823.5 45 57740.5 46 55273.5 47 52719.5 48 51913.5 49 51147.5 50 49579.5 51 47291.0 52 43719.0 53 39107.0 54 34841.0 55 31291.5 56 27591.0 57 24077.0 58 20756.0 59 17417.0 60 14181.0 61 11383.0 62 9064.0 63 6924.0 64 5173.5 65 3803.0 66 2723.5 67 2006.0 68 1496.0 69 1048.5 70 683.0 71 429.0 72 275.0 73 193.5 74 137.5 75 100.0 76 67.5 77 41.0 78 25.5 79 14.0 80 12.5 81 13.0 82 10.0 83 6.5 84 6.0 85 6.0 86 5.5 87 6.5 88 5.0 89 4.5 90 5.5 91 5.5 92 6.0 93 4.5 94 3.5 95 3.0 96 2.5 97 6.5 98 6.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.106320194058856E-4 3 0.041522977319600224 4 0.04717132349910466 5 0.04823992953306496 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.579740145544142E-4 12-13 0.001717402554579053 14-15 0.0 16-17 1.526580048514714E-4 18-19 0.0065261297074004024 20-21 0.0018318960582176568 22-23 4.96138515767282E-4 24-25 0.0 26-27 0.0 28-29 0.0010686060339602998 30-31 0.0 32-33 0.0011830995375989033 34-35 7.63290024257357E-5 36-37 3.816450121286785E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 7.63290024257357E-5 46-47 0.0 48-49 1.1449350363860355E-4 50-51 0.0 52-53 0.0 54-55 0.001297593041237507 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.63290024257357E-5 66-67 0.0 68-69 3.4348051091581065E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.6715150849007495E-4 80-81 0.0 82-83 4.579740145544142E-4 84-85 7.63290024257357E-5 86-87 0.0010304415327474319 88-89 19.275324818069823 90-91 26.253818358346347 92-93 40.30102631976661 94-95 44.77230295286379 96-97 48.352438482640494 98-99 93.04211529037842 100-101 51.594131215661484 102-103 53.124069740282934 104-105 74.83245783967551 106-107 81.62821974814483 108-109 12.617222265475322 110-111 39.124071266862984 112-113 52.00294935265373 114-115 86.79699080540837 116-117 66.42069645634973 118-119 71.79547949116034 120-121 80.37917195245008 122-123 75.85435052415126 124-125 49.96366739484535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1310118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.17091256076033 #Duplication Level Percentage of deduplicated Percentage of total 1 68.01351188641061 28.681918627120552 2 14.543221952133969 12.266018825903437 3 6.235620813610414 7.888854602784659 4 3.3595023797997983 5.666931244248142 5 1.9719984613434771 4.158048734163485 6 1.277510193633826 3.2324262402727246 7 0.8861770102955974 2.6159625250172147 8 0.6548166913456711 2.209137394725175 9 0.4882015807713131 1.8529115557258817 >10 2.3589253655039806 17.320623954640038 >50 0.13748597136660842 3.9590950551738104 >100 0.06469203496604611 4.915508385692882 >500 0.00471145933234656 1.4220606977930739 >1k 0.003261779537778388 2.6574324663202824 >5k 3.624199486420431E-4 1.153069690418598 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9784 0.746802959733398 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5305 0.40492535786852785 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4298 0.328062052425812 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3440 0.2625717683445308 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3064 0.2338720634324542 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2638 0.2013559083990908 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2425 0.18509783088240908 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2347 0.17914416869320168 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2077 0.15853533803825304 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1981 0.1512077538053824 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1914 0.14609371064285812 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1644 0.1254848799879095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.63290024257357E-5 0.0 0.0 0.0 0.0 8 7.63290024257357E-5 0.0 0.0 0.0 0.0 9 7.63290024257357E-5 0.0 0.0 0.0 0.0 10-11 7.63290024257357E-5 0.0 0.0 0.0 0.0 12-13 7.63290024257357E-5 0.0 0.0 7.63290024257357E-5 0.0 14-15 1.526580048514714E-4 0.0 0.0 7.63290024257357E-5 0.0 16-17 1.526580048514714E-4 0.0 0.0 7.63290024257357E-5 0.0 18-19 1.526580048514714E-4 0.0 0.0 7.63290024257357E-5 0.0 20-21 1.526580048514714E-4 0.0 0.0 1.9082250606433925E-4 0.0 22-23 1.526580048514714E-4 0.0 0.0 3.053160097029428E-4 0.0 24-25 3.053160097029428E-4 0.0 0.0 4.1980951334154635E-4 0.0 26-27 3.4348051091581065E-4 0.0 0.0 0.0014502510460889782 0.0 28-29 4.1980951334154635E-4 0.0 0.0 0.0043889176394798024 0.0 30-31 4.579740145544142E-4 0.0 0.0 0.012250804889330578 0.0 32-33 4.579740145544142E-4 0.0 0.0 0.028547046907225152 0.0 34-35 4.579740145544142E-4 0.0 0.0 0.05159840563979733 0.0 36-37 4.579740145544142E-4 0.0 0.0 0.08690056926170009 0.0 38-39 5.343030169801499E-4 0.0 0.0 0.14231542502278421 0.0 40-41 5.343030169801499E-4 0.0 0.0 0.20047812487119482 0.0 42-43 6.106320194058856E-4 0.0 0.0 0.26344955187242675 0.0 44-45 6.869610218316213E-4 0.0 0.0 0.3357712816708114 0.0 46-47 8.777835278959605E-4 0.0 0.0 0.4250762145089221 0.0 48-49 9.92277031534564E-4 0.0 0.0 0.51461013435431 0.0 50-51 9.92277031534564E-4 0.0 0.0 0.6158223915708356 0.0 52-53 9.92277031534564E-4 0.0 0.0 0.7150119302230791 0.0 54-55 0.0010686060339602998 0.0 0.0 0.8215672176094062 0.0 56-57 0.0010686060339602998 0.0 0.0 0.9496472836797907 0.0 58-59 0.0010686060339602998 0.0 0.0 1.0911994186783176 0.0 60-61 0.0010686060339602998 0.0 0.0 1.2399264799048635 0.0 62-63 0.0010686060339602998 0.0 0.0 1.3888825281386867 0.0 64-65 0.0010686060339602998 0.0 0.0 1.54276179702897 0.0 66-67 0.0010686060339602998 0.0 0.0 1.7285847534344234 0.0 68-69 0.0011449350363860354 0.0 0.0 1.942382289228909 0.0 70-71 0.0011449350363860354 0.0 0.0 2.16503398930478 0.0 72-73 0.0011449350363860354 0.0 0.0 2.38833448590127 0.0 74-75 0.0011449350363860354 0.0 0.0 2.629228817556892 0.0 76-77 0.0011449350363860354 0.0 0.0 2.879473452009666 0.0 78-79 0.0011449350363860354 3.816450121286785E-5 0.0 2.949963285749833 0.0 80-81 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 82-83 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 84-85 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 86-87 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 88-89 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 90-91 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 92-93 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 94-95 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 96-97 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 98-99 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 100-101 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 102-103 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 104-105 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 106-107 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 108-109 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 110-111 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 112-113 0.0011449350363860354 7.63290024257357E-5 0.0 2.951184549788645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 450 0.0 1428.0974 108-109 CCGCGCC 495 0.0 1198.4034 108-109 CGTCGCT 135 0.0 1123.2783 110-111 CCGCGCG 120 0.0 1029.8779 108-109 TTGCGAC 30 0.007988587 1010.95044 110-111 CGCGTAG 30 0.007988587 1010.95044 110-111 CCTCGCC 555 0.0 801.6347 108-109 CGTGTCG 65 4.923703E-5 760.5252 108-109 CGTTGTG 90 7.062443E-5 673.967 110-111 GGTCGCC 150 1.0932126E-9 659.1219 108-109 CGTCGCG 75 7.561443E-5 659.1219 108-109 TTGTTCG 300 0.0 659.1219 108-109 CCGCTCG 205 7.2759576E-12 602.85535 108-109 CGTCGCC 205 7.2759576E-12 602.85535 108-109 CCGTTCG 85 1.1003904E-4 581.5781 108-109 CGGCGCG 105 1.12107715E-4 577.686 110-111 CGGTGCG 50 2.3775219E-4 494.34143 108-109 CGGTACT 130 2.1263337E-4 466.59253 110-111 CTCCGCC 270 7.376002E-9 449.3113 110-111 CATCGCC 295 6.366463E-11 418.93338 108-109 >>END_MODULE