FastQCFastQC Report
Fri 27 May 2016
SRR939956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310118
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99390.7586339551093871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71450.5453707223318816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54130.4131688901305074No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52880.40362776482729035No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43780.33416837261987087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.26150316231057047No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32670.2493668509248785No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29930.22845270426022696No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26800.20456172650097168No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24640.18807466197701275No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23100.17631999560344946No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20970.16006191808676776No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20310.15502420392666919No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19420.14823092271077873No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16500.1259428540024639No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.10228086325048584No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT851.6999658E-435.0371973
GTGTACG1301.720955E-632.0749631
GTACATG67550.029.1004711
TACATGG66850.028.6010442
GAGTACT73950.028.3999312-13
ACATGGG63900.028.1503873
GTACTTT77650.026.89498514-15
AGTACTT78400.025.6875912-13
GGACCGA5150.025.4153586
AGAGTAC112150.025.06589910-11
TGTACGC1250.00162357823.8252942
CATGGGG26550.023.331744
ACTTTTT90050.023.02764716-17
CATGGGA37700.022.7509444
TACTTTT87850.022.51933114-15
GTAAGGT8500.022.4238074
TAAGGTG9150.022.1327865
AGGACCG6250.021.9192715
TACGCTC1400.0031365121.2725854
GGTAAGG8450.021.1467113
Produced by FastQC (version 0.11.5)