##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939956_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1310118 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8322403020186 34.0 31.0 34.0 31.0 34.0 2 32.87494943203589 34.0 31.0 34.0 31.0 34.0 3 32.913068135847304 34.0 31.0 34.0 31.0 34.0 4 36.338807649387306 37.0 37.0 37.0 35.0 37.0 5 36.24472375770732 37.0 37.0 37.0 35.0 37.0 6 36.384945478193565 37.0 37.0 37.0 35.0 37.0 7 36.37479066771085 37.0 37.0 37.0 35.0 37.0 8 36.396958899885355 37.0 37.0 37.0 35.0 37.0 9 38.22271581643791 39.0 39.0 39.0 37.0 39.0 10-11 38.22097742340766 39.0 39.0 39.0 37.0 39.0 12-13 38.20809194286316 39.0 39.0 39.0 37.0 39.0 14-15 39.685862647486715 41.0 40.0 41.0 37.0 41.0 16-17 39.70595129599013 41.0 40.0 41.0 37.0 41.0 18-19 39.792104222673075 41.0 40.0 41.0 37.5 41.0 20-21 39.81309393505013 41.0 40.0 41.0 37.5 41.0 22-23 39.76028914952699 41.0 40.0 41.0 37.0 41.0 24-25 39.70437662867009 41.0 40.0 41.0 37.0 41.0 26-27 39.590888759638446 41.0 40.0 41.0 36.5 41.0 28-29 39.50766152361848 41.0 40.0 41.0 36.0 41.0 30-31 39.355144727421504 41.0 39.0 41.0 36.0 41.0 32-33 39.14670205279219 41.0 39.0 41.0 35.0 41.0 34-35 39.00083885573666 41.0 39.0 41.0 35.0 41.0 36-37 38.9615011777565 41.0 39.0 41.0 35.0 41.0 38-39 38.9984455598656 41.0 39.0 41.0 35.0 41.0 40-41 38.93579013493441 41.0 39.0 41.0 35.0 41.0 42-43 38.771711021449974 41.0 39.0 41.0 35.0 41.0 44-45 38.54741214150175 41.0 39.0 41.0 35.0 41.0 46-47 38.24621751628479 41.0 39.0 41.0 34.5 41.0 48-49 37.909599745977076 41.0 38.0 41.0 34.0 41.0 50-51 37.69431379463529 41.0 38.0 41.0 33.0 41.0 52-53 37.56350305850313 41.0 38.0 41.0 33.0 41.0 54-55 37.40451814264058 40.0 37.5 41.0 33.0 41.0 56-57 37.22065951311256 40.0 37.0 41.0 33.0 41.0 58-59 37.01589627804518 40.0 36.5 41.0 33.0 41.0 60-61 36.758800352334674 40.0 36.0 41.0 32.0 41.0 62-63 36.516151598558295 39.0 35.5 41.0 32.0 41.0 64-65 36.229909443271524 39.0 35.0 41.0 31.5 41.0 66-67 35.90406627494623 38.5 35.0 41.0 31.0 41.0 68-69 35.53557427651555 37.0 35.0 40.0 31.0 41.0 70-71 35.14101477882145 37.0 35.0 39.5 31.0 41.0 72-73 34.7698081394195 36.0 35.0 39.0 30.5 41.0 74-75 34.42346681749277 36.0 35.0 39.0 30.0 41.0 76-77 34.06197266200449 35.0 35.0 37.0 30.0 39.5 78-79 33.71143553481443 35.0 35.0 37.0 29.5 39.0 80-81 33.457094704446476 35.0 35.0 36.5 29.5 39.0 82-83 33.24731092924454 35.0 35.0 36.0 29.5 38.0 84-85 33.0242447626855 35.0 34.5 36.0 29.0 37.0 86-87 32.877081301073645 35.0 34.5 36.0 29.5 37.0 88-89 32.71065163595951 35.0 34.0 35.0 29.0 36.0 90-91 32.57977105879012 35.0 34.0 35.0 29.0 36.0 92-93 32.4582472723831 35.0 34.0 35.0 29.0 36.0 94-95 32.37180849358607 35.0 34.0 35.0 29.0 36.0 96-97 32.315016280976224 35.0 34.0 35.0 29.0 36.0 98-99 32.26462769002487 35.0 34.0 35.0 29.0 35.0 100-101 32.210401276831554 35.0 34.0 35.0 29.0 35.0 102-103 31.360797271696136 34.5 32.5 35.0 26.0 35.0 104-105 32.04367698176805 35.0 34.0 35.0 28.0 35.0 106-107 32.08774018828838 35.0 34.0 35.0 29.0 35.0 108-109 32.106208753715315 35.0 34.0 35.0 29.0 35.0 110-111 32.07244919923244 35.0 34.0 35.0 29.0 35.0 112-113 31.98293169012257 35.0 34.0 35.0 27.0 35.0 114-115 31.922540946693353 35.0 34.0 35.0 27.0 35.0 116-117 31.854960774525654 35.0 34.0 35.0 27.0 35.0 118-119 31.759235427648502 35.0 34.0 35.0 26.0 35.0 120-121 31.664819886452975 35.0 34.0 35.0 25.0 35.0 122-123 31.486159643635155 35.0 34.0 35.0 25.0 35.0 124-125 29.495108455879546 33.5 29.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07655158669749085 1101 2 0.14408500651062894 1101 3 0.17221436500999943 1101 4 0.09199463209854031 1101 5 0.0943970604643738 1101 6 0.05102195891466721 1101 7 0.07184189416695119 1101 8 0.12051743296798634 1101 9 0.11249720196379798 1101 10-11 0.08219436698741589 1101 12-13 0.06158865371616429 1101 14-15 0.16855288283782244 1101 16-17 0.19777145897334947 1101 18-19 0.23479733334520603 1101 20-21 0.22296081681709268 1101 22-23 0.2293366563844259 1101 24-25 0.2743281248769307 1101 26-27 0.20412648826744828 1101 28-29 0.20869970386137737 1101 30-31 0.2736494462996433 1101 32-33 0.30658565344433697 1101 34-35 0.3515190324059603 1101 36-37 0.349424716366066 1101 38-39 0.25201885686232117 1101 40-41 0.3562211905820618 1101 42-43 0.3438639499511922 1101 44-45 0.33984881528324706 1101 46-47 0.3174648309207484 1101 48-49 0.3086130147312076 1101 50-51 0.3171527057434602 1101 52-53 0.32067440154204974 1101 54-55 0.21038536031844757 1101 56-57 0.26689434379942156 1101 58-59 0.12092838271684059 1101 60-61 0.15059709048065173 1101 62-63 0.14609220803896505 1101 64-65 0.1647649303317138 1101 66-67 0.1900848604371248 1101 68-69 0.18820488437524574 1101 70-71 0.166544500557265 1101 72-73 0.17255625401182328 1101 74-75 0.23953589137205 1101 76-77 0.20394518298955688 1101 78-79 0.18038258461056245 1101 80-81 0.1673785715072924 1101 82-83 0.2213892186311739 1101 84-85 0.23873305332950423 1101 86-87 0.3377659215684048 1101 88-89 0.35963927352869973 1101 90-91 0.30911886801595045 1101 92-93 0.2845049403926794 1101 94-95 0.2760601128908817 1101 96-97 0.24361226404216296 1101 98-99 0.28041935293475007 1101 100-101 0.27141597982813437 1101 102-103 0.3186708925115056 1101 104-105 0.3603700827642058 1101 106-107 0.40192229509230515 1101 108-109 0.3304445757220691 1101 110-111 0.3079047763153824 1101 112-113 0.3071460441894267 1101 114-115 0.34639994378922623 1101 116-117 0.3717552491944254 1101 118-119 0.3448127767336828 1101 120-121 0.3583038876555271 1101 122-123 0.3789623026254958 1101 124-125 0.35485477773081087 1102 1 0.04478642934930832 1102 2 0.12124225104194153 1102 3 0.08907228769814424 1102 4 0.06145413024274404 1102 5 0.033760264286527786 1102 6 0.04414136930320467 1102 7 0.015663214154315597 1102 8 -0.0026779739261115765 1102 9 0.07169272978105568 1102 10-11 0.12381507051343732 1102 12-13 0.09354491284708644 1102 14-15 0.10354465265935175 1102 16-17 0.1366663940203381 1102 18-19 0.12425320379455229 1102 20-21 0.06450581997589921 1102 22-23 0.09976733409705218 1102 24-25 0.13433180192818384 1102 26-27 0.04459135762107991 1102 28-29 0.08639348322842721 1102 30-31 0.07085899009850749 1102 32-33 0.013696799104920387 1102 34-35 0.005150367298959679 1102 36-37 0.1416629917549912 1102 38-39 0.09772892260502886 1102 40-41 -0.056294757702318066 1102 42-43 -0.02611974393059313 1102 44-45 0.0478341860484619 1102 46-47 -0.07892391606851135 1102 48-49 -0.037594779383532284 1102 50-51 0.022257822520685977 1102 52-53 0.02117086515651323 1102 54-55 0.046845729602985386 1102 56-57 -0.04484865252846504 1102 58-59 0.012192239524068782 1102 60-61 0.06208164439757269 1102 62-63 0.1550875648363359 1102 64-65 0.14772479001149463 1102 66-67 0.11566421194348209 1102 68-69 0.07192168603165072 1102 70-71 0.07566673413145963 1102 72-73 0.12109727904538659 1102 74-75 0.12079181413916729 1102 76-77 0.0309075314654379 1102 78-79 0.08103680359499776 1102 80-81 0.1178346245752877 1102 82-83 0.07467965508975283 1102 84-85 0.038142349852840596 1102 86-87 0.02044488167857139 1102 88-89 0.05139194370953959 1102 90-91 0.12887564588257305 1102 92-93 0.10449338482725068 1102 94-95 0.13706726709998662 1102 96-97 0.021507571729920016 1102 98-99 0.10300317242855073 1102 100-101 0.17470521322464094 1102 102-103 0.059238731639275954 1102 104-105 0.03750400639999896 1102 106-107 0.04423096503623469 1102 108-109 0.05559903038329139 1102 110-111 0.07640401499302385 1102 112-113 0.058865805995477416 1102 114-115 0.04631986304216085 1102 116-117 0.0526421095355758 1102 118-119 0.05891001129500495 1102 120-121 0.1333633621086605 1102 122-123 0.17812574261799696 1102 124-125 0.09715934492460931 1103 1 0.043030958618672344 1103 2 0.06746093015594568 1103 3 0.11440956205433395 1103 4 0.06759500155666842 1103 5 -0.02329074741739845 1103 6 0.0671665094072793 1103 7 0.10216824884675191 1103 8 0.06051897237685466 1103 9 0.10608941255492965 1103 10-11 0.09129612191352265 1103 12-13 0.1240020619673956 1103 14-15 0.17611522889693276 1103 16-17 0.11937706242162704 1103 18-19 0.03448175452747648 1103 20-21 0.1405639572111781 1103 22-23 0.04906225675388498 1103 24-25 0.18592757068974208 1103 26-27 0.19324479183887888 1103 28-29 0.2983414064475838 1103 30-31 0.13526621046220555 1103 32-33 0.24166108447882095 1103 34-35 0.2605207257557183 1103 36-37 0.34748121267148235 1103 38-39 0.23404625834015036 1103 40-41 0.25695818257713654 1103 42-43 0.21123425475414592 1103 44-45 0.17690238671181646 1103 46-47 0.28647460616705445 1103 48-49 0.32382429859821116 1103 50-51 0.3485374040193179 1103 52-53 0.3720714150888398 1103 54-55 0.33009364233814864 1103 56-57 0.355641264981692 1103 58-59 0.36005630759368756 1103 60-61 0.38883985525897913 1103 62-63 0.3321009056990647 1103 64-65 0.244400475282454 1103 66-67 0.26922009750609277 1103 68-69 0.22671896996638452 1103 70-71 0.33959329981834685 1103 72-73 0.2800069929280795 1103 74-75 0.19989611304692545 1103 76-77 0.20742809555112984 1103 78-79 0.28421571268937385 1103 80-81 0.12885486461073725 1103 82-83 0.22045595200555113 1103 84-85 0.19996881379748999 1103 86-87 0.17785020420386388 1103 88-89 0.1493945075188421 1103 90-91 0.2028707922770323 1103 92-93 0.2523986442104089 1103 94-95 0.25144881362979987 1103 96-97 0.19271555837714516 1103 98-99 0.2927153812598746 1103 100-101 0.2827691202222198 1103 102-103 0.18715726874302874 1103 104-105 0.14096583399573603 1103 106-107 0.22365874829426957 1103 108-109 0.2218296588501545 1103 110-111 0.2687556845666208 1103 112-113 0.2662170109381954 1103 114-115 0.23979587974980987 1103 116-117 0.23640272456388445 1103 118-119 0.2772519700834408 1103 120-121 0.24808221277868014 1103 122-123 0.25139341407869864 1103 124-125 0.3401822869672664 1104 1 0.030808150842098314 1104 2 0.06211125832208353 1104 3 0.06826896610831312 1104 4 0.022302436613834686 1104 5 0.004637030718356527 1104 6 -0.027185569591928527 1104 7 -0.06341785909366848 1104 8 -0.09209208861693696 1104 9 -0.08916224668619321 1104 10-11 0.014220373298904576 1104 12-13 0.08008740357315247 1104 14-15 0.056399025866575414 1104 16-17 0.07655466105556741 1104 18-19 0.04031786476920729 1104 20-21 0.10492896379291494 1104 22-23 0.03500660313714832 1104 24-25 0.07207250459624959 1104 26-27 0.1300231887956329 1104 28-29 0.07448883727034428 1104 30-31 0.018343935898066377 1104 32-33 0.04986598579885282 1104 34-35 0.02932267772287389 1104 36-37 0.03952183186068936 1104 38-39 0.007241689788870076 1104 40-41 -0.04339092543693823 1104 42-43 0.001119039904281749 1104 44-45 -0.060142127436606074 1104 46-47 -0.03308781213660694 1104 48-49 -0.028844410093363138 1104 50-51 -0.036138721779231275 1104 52-53 -0.036458027811164584 1104 54-55 -0.15008694108876597 1104 56-57 -0.03942108313216153 1104 58-59 -0.0012725006063192268 1104 60-61 -0.04344768823569467 1104 62-63 -0.09255982436702936 1104 64-65 -0.11607972658804044 1104 66-67 -0.11170475682128256 1104 68-69 -0.10217107021440341 1104 70-71 -0.03725651814839637 1104 72-73 -0.09821828645037556 1104 74-75 -0.17470835010457364 1104 76-77 -0.12336972672365931 1104 78-79 -0.16318507803912752 1104 80-81 -0.1949412362876899 1104 82-83 -0.08060210187259997 1104 84-85 -0.12208649347279987 1104 86-87 -0.10475267564957846 1104 88-89 0.01536073356225387 1104 90-91 -0.042326095897934124 1104 92-93 -0.07093029171203824 1104 94-95 -0.09960279659352267 1104 96-97 -0.07739770611647856 1104 98-99 -0.07629417721169318 1104 100-101 -0.0224325776808314 1104 102-103 -0.045131598002186024 1104 104-105 -0.027125388595713673 1104 106-107 -0.0729419648802434 1104 108-109 -0.03596486220218509 1104 110-111 -0.058056564591105086 1104 112-113 0.01638083510926336 1104 114-115 0.03422588669975468 1104 116-117 -0.07271349365509039 1104 118-119 0.10237232835065058 1104 120-121 0.08922282023122818 1104 122-123 0.14533646482708562 1104 124-125 0.06633199032901516 1105 1 -0.06809602956430183 1105 2 -0.010093615991415561 1105 3 -0.09394226339799161 1105 4 -0.03273258913316823 1105 5 -0.2305439728912475 1105 6 -0.07918376270705352 1105 7 -0.16777045818428604 1105 8 -0.014832489957498751 1105 9 -0.008569850539736024 1105 10-11 0.035323139459741526 1105 12-13 0.024589550984195796 1105 14-15 0.008383864469948321 1105 16-17 0.024998610559414658 1105 18-19 0.003827424906013732 1105 20-21 0.052669094433909436 1105 22-23 0.0628471508220585 1105 24-25 -0.027075583057097674 1105 26-27 0.018846878422337454 1105 28-29 0.02179793091546145 1105 30-31 -0.07247271331888072 1105 32-33 0.0260953713330494 1105 34-35 0.1069226637297902 1105 36-37 0.06021160766314182 1105 38-39 0.07103710866381618 1105 40-41 0.060126947258751784 1105 42-43 0.08737544264304375 1105 44-45 0.008830038817905006 1105 46-47 0.03315695190916301 1105 48-49 -0.07890883658927095 1105 50-51 -0.07316556323730339 1105 52-53 -0.06409659197626638 1105 54-55 -0.10295648139093316 1105 56-57 -0.02928942319411476 1105 58-59 -0.23883982497575573 1105 60-61 -0.09870093947625946 1105 62-63 -0.15552870724407342 1105 64-65 -0.06149475621646161 1105 66-67 -0.07072486882704254 1105 68-69 -0.11715977440753278 1105 70-71 -0.05347392071529811 1105 72-73 -0.09836529713593478 1105 74-75 -0.1133701891568819 1105 76-77 -0.05989775912097883 1105 78-79 -0.0815813760907318 1105 80-81 -0.14508892719265276 1105 82-83 -0.09782969967913857 1105 84-85 -0.15136771811206273 1105 86-87 -0.21409614175341574 1105 88-89 -0.03226198287870119 1105 90-91 -0.12824488769498998 1105 92-93 -0.17540630203915697 1105 94-95 -0.07198325080795343 1105 96-97 0.005927645050270769 1105 98-99 -0.1251634998436444 1105 100-101 -0.019670886860822634 1105 102-103 -0.052974944304111204 1105 104-105 -0.05361174436601601 1105 106-107 0.010275245281739842 1105 108-109 0.03899975944727885 1105 110-111 -0.0838947684342628 1105 112-113 -0.19558261406404753 1105 114-115 -0.12186892089393453 1105 116-117 -0.2094173280658076 1105 118-119 -0.17655247519781625 1105 120-121 -0.4590213946414785 1105 122-123 -0.36214525462322555 1105 124-125 -0.3183398621083491 1106 1 0.05527565696047532 1106 2 0.08992566269915869 1106 3 0.02282242758739983 1106 4 0.04607350038731539 1106 5 -0.010439666424240102 1106 6 0.07803276562771799 1106 7 0.04796445372111435 1106 8 0.0205257423218157 1106 9 0.07077826984097157 1106 10-11 0.0843488230775975 1106 12-13 0.09339114037566532 1106 14-15 -0.0354961059252048 1106 16-17 0.026187286357341577 1106 18-19 0.016431498676311662 1106 20-21 0.07461153255978559 1106 22-23 0.09220485751176 1106 24-25 0.12463511566151908 1106 26-27 0.05665551978380279 1106 28-29 0.06321330502149891 1106 30-31 0.015188845157730668 1106 32-33 0.16716778628472184 1106 34-35 0.16847392741215117 1106 36-37 0.12653056842768962 1106 38-39 0.06112966494128358 1106 40-41 0.08564230749580304 1106 42-43 0.16545002916551255 1106 44-45 0.12534227707387657 1106 46-47 0.22510906650573048 1106 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78-79 -0.3615686257899213 2216 80-81 -0.33670092640065974 2216 82-83 -0.3163972708338605 2216 84-85 -0.30129678640449953 2216 86-87 -0.28035342802813545 2216 88-89 -0.3407277692088968 2216 90-91 -0.3117000669063472 2216 92-93 -0.2929688622821587 2216 94-95 -0.1834638522914318 2216 96-97 -0.1738344073565159 2216 98-99 -0.2020844762642966 2216 100-101 -0.2575150198730043 2216 102-103 -0.23402519049805903 2216 104-105 -0.2962927235717103 2216 106-107 -0.3527427900840294 2216 108-109 -0.34428994871008456 2216 110-111 -0.32727505594701967 2216 112-113 -0.2891643824393846 2216 114-115 -0.37382476481807103 2216 116-117 -0.41603334166032724 2216 118-119 -0.4159542772113234 2216 120-121 -0.4798480101328728 2216 122-123 -0.5534983313404318 2216 124-125 -0.5765574962530486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103.0 3 6.0 4 28.0 5 52.0 6 161.0 7 328.0 8 604.0 9 1257.0 10 2503.0 11 3551.0 12 4366.0 13 7170.0 14 12520.0 15 13742.0 16 5505.0 17 4109.0 18 3729.0 19 3283.0 20 2966.0 21 3016.0 22 2930.0 23 2831.0 24 3375.0 25 4390.0 26 5403.0 27 6705.0 28 8278.0 29 10452.0 30 13387.0 31 17326.0 32 22649.0 33 29932.0 34 42933.0 35 74896.0 36 179528.0 37 510463.0 38 304261.0 39 1380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08884199886863 19.319485678907352 14.704779906574519 21.88689241564949 2 14.883086866984502 21.59614630132553 39.745198524102406 23.775568307587562 3 19.618156532465015 26.729424372461107 29.65824452453901 23.994174570534867 4 12.138830242772025 17.327294182661408 38.70750573612453 31.82636983844203 5 12.301736820987065 39.03555801822802 34.398838297953645 14.263866862831268 6 29.614660664153913 37.897502362382625 17.63818220954143 14.849654763922029 7 26.42235279570237 34.27111145713592 21.7849842533268 17.521551493834906 8 25.74607783421035 33.470114905680255 20.533646587559286 20.250160672550106 9 26.171459364728978 17.042358016606137 21.4347104611951 35.351472157469786 10-11 24.45424763265599 26.913758913319256 27.367229516730557 21.264763937294198 12-13 26.658896374219726 24.590304079479864 27.676819950569335 21.07397959573107 14-15 22.890037385945387 25.493047191169037 27.701359724849212 23.915555698036357 16-17 20.849648657601836 28.84816482179468 28.17150821529053 22.130678305312955 18-19 20.859609592418394 27.51546043944133 30.8063090500245 20.818620918115773 20-21 22.18481929807688 25.734776628210355 30.77974410293285 21.30065997077992 22-23 21.657992488442805 25.82766620780092 30.849059376107967 21.66528192764831 24-25 21.96195304545087 25.979644581633103 30.900117394005733 21.158284978910295 26-27 21.330330550377905 25.794966560264037 31.70874684570397 21.165956043654084 28-29 20.855678648793468 27.161293868185922 30.89637727288687 21.086650210133744 30-31 21.19534270958799 26.181000490032197 30.682579737092386 21.94107706328743 32-33 21.373685423755724 26.01456510024288 30.76131310309453 21.850436372906866 34-35 22.214373056472773 25.924344219375662 30.536486026449527 21.324796697702038 36-37 21.935161565599433 25.379316977554694 31.090710913062793 21.59481054378308 38-39 21.365899865508297 26.370639896558934 30.67987005750627 21.583590180426494 40-41 21.753803855835887 26.254276332360902 30.519350165404944 21.472569646398263 42-43 21.4827595682221 26.251108678760232 29.71037723319579 22.55575451982188 44-45 21.785556720844994 25.826375944762226 29.880667237607604 22.507400096785172 46-47 22.770620661650327 26.584475596854634 29.088868330944234 21.556035410550805 48-49 22.217120900560104 26.659163525728218 28.956284853730736 22.16743071998095 50-51 21.62053341760055 26.93669577854819 29.51302859742405 21.929742206427207 52-53 22.457328271193894 27.3353239937166 28.190475972393326 22.016871762696187 54-55 21.840284615584245 26.976654011318065 28.588302733036258 22.59475864006143 56-57 22.31234133108621 26.72030305667123 28.44022446833033 22.52713114391223 58-59 22.881374044170066 26.298508989266615 28.408013629306673 22.412103337256646 60-61 22.06656194327534 27.054891238804444 29.18706559256494 21.691481225355275 62-63 21.637020482124512 26.916277770399304 29.385597327874287 22.0611044196019 64-65 21.531571965273358 27.46806012893495 28.764889880148196 22.23547802564349 66-67 21.899897566478746 26.781786068125164 28.29432158019354 23.023994785202554 68-69 21.863717619328945 26.730302155989005 28.81534335075161 22.59063687393044 70-71 22.564532355100837 26.719692424651825 28.18127832760102 22.53449689264631 72-73 22.212083186400005 26.377662164782105 28.91499086341841 22.495263785399484 74-75 21.6215638591333 27.939239060909017 28.09403427782841 22.345162802129273 76-77 22.097742340766253 26.758811038395013 28.115139246999128 23.028307373839606 78-79 22.29722818860591 26.98375260854366 28.22909844762075 22.48992075522968 80-81 22.599033064197272 26.996766703457247 27.759560589198834 22.644639643146647 82-83 22.286618457268734 26.69954156801143 28.524110041996213 22.489729932723616 84-85 22.039617805419056 27.187016742003394 28.20009342669897 22.573272025878584 86-87 22.045800454615538 27.351391248727214 27.889167235317736 22.713641061339512 88-89 21.951381478614902 27.349254036659293 27.293266713380014 23.406097771345788 90-91 22.14856849751872 26.75497735112981 27.70859993000585 23.38785422134562 92-93 22.501789915106883 27.01000978537811 27.837835981186426 22.650364318328577 94-95 22.072039752387205 26.938310523543823 27.942997152910827 23.04665257115815 96-97 21.586414353516247 26.76098641496415 28.6026525854923 23.049946646027305 98-99 21.889326174603198 27.15651147773227 27.533314199963055 23.420848147701477 100-101 21.395379191324135 26.597166512609178 28.046464552402572 23.960989743664115 102-103 22.049964297040987 26.177233991964833 27.856322591405107 23.916479119589074 104-105 22.442169027383244 26.446857256698873 27.48361848560728 23.627355230310602 106-107 21.873924171607367 26.612454457393188 28.07119188236668 23.44242948863276 108-109 21.49364750422673 26.79302961953722 27.762104853390735 23.95121802284532 110-111 21.352672824374046 27.39634010665879 27.448856564805347 23.802130504161813 112-113 21.635893270717844 26.866289162034374 27.294951320833643 24.20286624641414 114-115 21.288463021074655 27.095500424790675 27.36682449694937 24.2492120571853 116-117 21.75854808417808 27.047214208648402 27.23628324021728 23.957954466956235 118-119 21.344301323051287 26.73878753438151 27.773123819999075 24.143787322568127 120-121 20.87378788989033 27.922345570455043 27.20721587162014 23.996650668034487 122-123 20.9521116181936 27.328031484403205 27.11717116667392 24.602685730729277 124-125 20.98709833772548 27.2896586335802 27.24401335010047 24.47922967859385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 258.5 2 315.5 3 393.0 4 233.0 5 181.5 6 461.0 7 1273.0 8 2242.0 9 2433.0 10 1732.5 11 1128.0 12 1108.0 13 1262.5 14 1425.5 15 1551.5 16 1657.0 17 1802.5 18 1978.5 19 2113.5 20 2370.0 21 2877.0 22 3371.0 23 3875.0 24 4609.5 25 5758.5 26 7376.0 27 9387.5 28 10911.0 29 12735.0 30 15330.0 31 18133.0 32 21864.5 33 24802.0 34 26502.5 35 28052.0 36 31559.0 37 35606.0 38 38111.5 39 40465.5 40 41042.5 41 41696.0 42 42986.5 43 43501.0 44 44471.0 45 46415.0 46 46962.5 47 54836.5 48 70332.0 49 66386.0 50 51180.0 51 44450.5 52 40892.0 53 39138.0 54 38418.0 55 35926.0 56 32763.5 57 29846.0 58 27083.0 59 23986.5 60 18958.5 61 15340.0 62 12934.5 63 9421.0 64 6518.0 65 5036.0 66 3804.0 67 2750.5 68 1954.0 69 1263.0 70 750.5 71 459.5 72 325.5 73 284.0 74 211.5 75 157.5 76 107.5 77 45.5 78 26.0 79 12.5 80 4.0 81 5.5 82 5.0 83 3.5 84 1.5 85 0.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 3.0 98 6.0 99 5.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015036813477869932 2 0.0 3 0.0 4 0.0 5 0.12922500110677054 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0045797401455441415 22-23 2.6715150849007495E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.487965206187535E-4 92-93 0.0 94-95 6.106320194058856E-4 96-97 0.0 98-99 0.0012212640388117712 100-101 7.63290024257357E-5 102-103 1.9082250606433922E-4 104-105 0.0021753765691334675 106-107 0.006182649196484591 108-109 2.289870072772071E-4 110-111 0.004007272627351124 112-113 0.008548848271682398 114-115 0.004656069147969878 116-117 0.0011067705351731677 118-119 1.9082250606433922E-4 120-121 4.579740145544142E-4 122-123 6.869610218316213E-4 124-125 0.0011830995375989033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1310118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.71053922328067 #Duplication Level Percentage of deduplicated Percentage of total 1 65.99267148007281 26.20604569258507 2 15.395070839708037 12.226931288508212 3 6.67921869926357 7.957061284139273 4 3.488183703701306 5.540706231353567 5 2.114852534035306 4.1990967252131775 6 1.421059359385379 3.3858620057689923 7 0.9537360920763213 2.651136214513863 8 0.6986626429942163 2.219541623077022 9 0.5033744277544109 1.7990342961604198 >10 2.5256531172775385 17.63831454776397 >50 0.1437694505294191 3.971697391534003 >100 0.07299108097075922 5.379871299194394 >500 0.006338694350345195 1.7522273821725016 >1k 0.0036495512926229914 2.9531270820211812 >5k 7.683265879206297E-4 2.1193469359943826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9939 0.7586339551093871 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7145 0.5453707223318816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5413 0.4131688901305074 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5288 0.40362776482729035 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4378 0.33416837261987087 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3426 0.26150316231057047 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3267 0.2493668509248785 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2993 0.22845270426022696 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2680 0.20456172650097168 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2464 0.18807466197701275 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2310 0.17631999560344946 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2097 0.16006191808676776 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2031 0.15502420392666919 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1942 0.14823092271077873 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1650 0.1259428540024639 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1340 0.10228086325048584 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.63290024257357E-5 0.0 0.0 0.0 0.0 12-13 7.63290024257357E-5 0.0 0.0 7.63290024257357E-5 0.0 14-15 7.63290024257357E-5 0.0 0.0 7.63290024257357E-5 0.0 16-17 7.63290024257357E-5 0.0 0.0 7.63290024257357E-5 0.0 18-19 7.63290024257357E-5 0.0 0.0 7.63290024257357E-5 0.0 20-21 7.63290024257357E-5 0.0 0.0 1.1449350363860355E-4 0.0 22-23 7.63290024257357E-5 0.0 0.0 2.289870072772071E-4 0.0 24-25 7.63290024257357E-5 0.0 0.0 3.4348051091581065E-4 0.0 26-27 7.63290024257357E-5 0.0 0.0 0.0013739220436632426 0.0 28-29 7.63290024257357E-5 0.0 0.0 0.004312588637054067 0.0 30-31 7.63290024257357E-5 0.0 0.0 0.012021817882053372 0.0 32-33 7.63290024257357E-5 0.0 0.0 0.02824173089752221 0.0 34-35 7.63290024257357E-5 0.0 0.0 0.05121676062766865 0.0 36-37 7.63290024257357E-5 0.0 0.0 0.08651892424957142 0.0 38-39 7.63290024257357E-5 0.0 0.0 0.14216276701793273 0.0 40-41 7.63290024257357E-5 0.0 0.0 0.20051628937240767 0.0 42-43 3.4348051091581065E-4 0.0 0.0 0.2635258808748525 0.0 44-45 4.579740145544142E-4 0.0 0.0 0.3360384331793014 0.0 46-47 6.106320194058856E-4 0.0 0.0 0.4252288725137736 0.0 48-49 6.869610218316213E-4 0.0 0.0 0.5148772858628001 0.0 50-51 6.869610218316213E-4 0.0 0.0 0.6167383395999444 0.0 52-53 6.869610218316213E-4 0.0 0.0 0.7158515492497622 0.0 54-55 6.869610218316213E-4 0.0 0.0 0.822483165638515 0.0 56-57 6.869610218316213E-4 0.0 0.0 0.9500289286919193 0.0 58-59 7.63290024257357E-4 0.0 0.0 1.0917718861965104 0.0 60-61 7.63290024257357E-4 0.0 0.0 1.2407660989315468 0.0 62-63 7.63290024257357E-4 0.0 0.0 1.3905236016908402 0.0 64-65 7.63290024257357E-4 0.0 0.0 1.5450898316029549 0.0 66-67 8.396190266830927E-4 0.0 0.0 1.7323630390544973 0.0 68-69 8.396190266830927E-4 0.0 0.0 1.9477253193987105 0.0 70-71 8.396190266830927E-4 0.0 0.0 2.171750941518245 0.0 72-73 8.396190266830927E-4 0.0 0.0 2.395471247628076 0.0 74-75 8.396190266830927E-4 0.0 0.0 2.6368998823006784 0.0 76-77 8.396190266830927E-4 0.0 0.0 2.8883657807922645 0.0 78-79 8.396190266830927E-4 0.0 0.0 3.207573668936691 0.0 80-81 9.159480291088284E-4 0.0 0.0 3.540329955011686 0.0 82-83 9.159480291088284E-4 0.0 0.0 3.875261617655814 0.0 84-85 9.541125303216962E-4 0.0 0.0 4.216719410007343 0.0 86-87 9.92277031534564E-4 0.0 0.0 4.572946864328252 0.0 88-89 9.92277031534564E-4 0.0 0.0 4.97920797973923 0.0 90-91 9.92277031534564E-4 0.0 0.0 5.422374167823051 0.0 92-93 9.92277031534564E-4 0.0 0.0 5.890156459189172 0.0 94-95 9.92277031534564E-4 0.0 0.0 6.342138647053166 0.0 96-97 9.92277031534564E-4 0.0 0.0 6.81675238413639 0.0 98-99 9.92277031534564E-4 0.0 0.0 7.323309808734786 0.0 100-101 9.92277031534564E-4 0.0 0.0 7.894441569385354 0.0 102-103 9.92277031534564E-4 0.0 0.0 8.509576999934357 0.0 104-105 0.001259428540024639 0.0 0.0 9.096699686593116 0.0 106-107 0.0014120865448761103 0.0 0.0 9.694508433591478 0.0 108-109 0.0014502510460889782 0.0 0.0 10.32132220151162 0.0 110-111 0.001526580048514714 0.0 0.0 10.981758895000297 0.0 112-113 0.001526580048514714 0.0 0.0 11.69482443566152 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 85 1.6999658E-4 35.037197 3 GTGTACG 130 1.720955E-6 32.074963 1 GTACATG 6755 0.0 29.100471 1 TACATGG 6685 0.0 28.601044 2 GAGTACT 7395 0.0 28.39993 12-13 ACATGGG 6390 0.0 28.150387 3 GTACTTT 7765 0.0 26.894985 14-15 AGTACTT 7840 0.0 25.68759 12-13 GGACCGA 515 0.0 25.415358 6 AGAGTAC 11215 0.0 25.065899 10-11 TGTACGC 125 0.001623578 23.825294 2 CATGGGG 2655 0.0 23.33174 4 ACTTTTT 9005 0.0 23.027647 16-17 CATGGGA 3770 0.0 22.750944 4 TACTTTT 8785 0.0 22.519331 14-15 GTAAGGT 850 0.0 22.423807 4 TAAGGTG 915 0.0 22.132786 5 AGGACCG 625 0.0 21.919271 5 TACGCTC 140 0.00313651 21.272585 4 GGTAAGG 845 0.0 21.146711 3 >>END_MODULE