FastQCFastQC Report
Fri 27 May 2016
SRR939955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174391
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC6800.01653.3616108-109
CCGTTCG1000.01222.0498108-109
CCGCTCG2450.01197.1101108-109
CCGCGCC5750.01105.1582108-109
CGTCGCC2350.01040.0424108-109
CGTCGCT1251.5643309E-10973.8061110-111
CCGCGCG2600.0940.0384108-109
CTCCGCC5350.0853.2179110-111
CGTCGCG1352.6089037E-7676.25415110-111
CCTCGCC8250.0592.509108-109
CGTCGTT1101.2798999E-4553.2989110-111
CGGCGCC4400.0499.9295108-109
CCTCGCG3750.0456.23193108-109
TTTTTCG12000.0437.3735792-93
CGACGCG1152.8233675E-4425.0608108-109
CATCGCG1152.8233675E-4425.0608108-109
GGTCGCC4150.0412.2578108-109
GGTGTCG3007.6397555E-11407.3499108-109
CGTTGCT4500.0405.75253110-111
CGTTGAC1553.5769586E-4392.6637110-111
Produced by FastQC (version 0.11.5)