FastQCFastQC Report
Fri 27 May 2016
SRR939955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174391
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39500.18166006021916023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.11897584197138418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68950.046.409861
TCAACGC78950.039.687514
ATCAACG79250.039.612443
CAACGCA81400.038.5661665
GGTATCA41500.038.481941
TATCAAC83100.037.9205742
AACGCAG84900.037.000446
ACGCAGA96450.032.1994827
CGCAGAG96800.031.9601358
GCAGAGT113700.026.84349
GTACATG59650.026.1734561
AGAGTAC89100.025.2403110-11
TACATGG60200.024.9358562
GAGTACT65050.023.59679612-13
ACATGGG59000.023.0198823
AGTACTT73000.022.16800912-13
GTACTTT70300.021.41365414-15
CAGAGTA108900.020.73311410-11
GTATAGG7250.020.5481281
GTAGGAC12000.019.3599223
Produced by FastQC (version 0.11.5)