FastQCFastQC Report
Fri 27 May 2016
SRR939954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699785
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9020.12889673256786013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG207.407234E-73073.2256110-111
CCGCGCT252.913148E-61948.8748108-109
CCGCGCC2250.01624.0624108-109
GGTCGCA305.033153E-61624.0623108-109
CGACGCG150.00309199471624.0623108-109
CCGCTCC3000.01542.8593108-109
CGCGTTG250.0053903271229.2902110-111
CGTTTCG250.0053903271229.2902110-111
CGACGCA200.0054961331218.0468108-109
CATCGCG401.1926808E-51218.0468108-109
CCGTTCG401.1926808E-51218.0468108-109
CGTCGCT300.00776123771024.4084110-111
CCTCGCC3900.0999.423108-109
CGTCGCC1001.5279511E-10974.4374108-109
CGTCTCT956.0288585E-8970.4923110-111
CCGCTCG859.8463715E-8859.7977108-109
CCGTTCC1551.3587851E-9628.66925108-109
CGTGTCT1554.2657666E-7594.8179110-111
CTGTTCG1254.5991328E-7584.6624108-109
CTCCGCC1604.8427864E-7576.2298110-111
Produced by FastQC (version 0.11.5)