FastQCFastQC Report
Fri 27 May 2016
SRR939954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699785
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.37340040155190524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.28322984916795874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.17148124066677622No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9450.1350414770250863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA2200.037.8603636
GTATCAA51750.035.339991
CGCGGAA609.2966366E-734.70533482-83
AGGACCG2600.034.3632435
TCGCGGA651.7376078E-632.0356982-83
ATCAACG59600.030.5809783
CAACGCA60150.030.3013525
TCAACGC60200.030.2761824
TATCAAC61050.030.0497782
AACGCAG61050.030.0154236
TAGAGCG800.005333142429.7814774
ATTCGCG703.097126E-629.74742780-81
TACCTGG8850.029.6132162
GTACATG43150.029.1299691
GGTATCA36200.029.1274911
GTACCTG9200.028.4907051
TACATGG43600.028.1421282
ACATGGG41700.027.7103423
ACCGAGT2803.6379788E-1227.622618
GTGTAGC5250.027.2326741
Produced by FastQC (version 0.11.5)