##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939954_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 699785 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85134434147631 34.0 31.0 34.0 31.0 34.0 2 32.89586515858442 34.0 31.0 34.0 31.0 34.0 3 32.93741792121866 34.0 31.0 34.0 31.0 34.0 4 36.35963474495738 37.0 37.0 37.0 35.0 37.0 5 36.2641568481748 37.0 37.0 37.0 35.0 37.0 6 36.39155169087648 37.0 37.0 37.0 35.0 37.0 7 36.38562415599077 37.0 37.0 37.0 35.0 37.0 8 36.40824967668641 37.0 37.0 37.0 35.0 37.0 9 38.24410068806848 39.0 39.0 39.0 37.0 39.0 10-11 38.23423837321463 39.0 39.0 39.0 37.0 39.0 12-13 38.2161421007881 39.0 39.0 39.0 37.0 39.0 14-15 39.732054130911635 41.0 40.0 41.0 37.0 41.0 16-17 39.73890909350729 41.0 40.0 41.0 37.0 41.0 18-19 39.8003086662332 41.0 40.0 41.0 37.5 41.0 20-21 39.82353222775566 41.0 40.0 41.0 37.5 41.0 22-23 39.778729895610795 41.0 40.0 41.0 37.0 41.0 24-25 39.737780175339566 41.0 40.0 41.0 37.0 41.0 26-27 39.64369842165808 41.0 40.0 41.0 37.0 41.0 28-29 39.563886050715574 41.0 40.0 41.0 37.0 41.0 30-31 39.4290875054481 41.0 39.0 41.0 36.0 41.0 32-33 39.257443357602696 41.0 39.0 41.0 35.0 41.0 34-35 39.14689726130169 41.0 39.0 41.0 35.0 41.0 36-37 39.12838586137171 41.0 39.0 41.0 35.0 41.0 38-39 39.183558521545905 41.0 39.0 41.0 35.0 41.0 40-41 39.11812771065399 41.0 39.0 41.0 35.0 41.0 42-43 38.97492587008867 41.0 39.0 41.0 35.0 41.0 44-45 38.783984366626896 41.0 39.0 41.0 35.0 41.0 46-47 38.524393206484845 41.0 39.0 41.0 35.0 41.0 48-49 38.24819408818423 41.0 39.0 41.0 35.0 41.0 50-51 38.0558857363333 41.0 39.0 41.0 34.0 41.0 52-53 37.917521095765125 41.0 38.0 41.0 34.0 41.0 54-55 37.76849675257401 41.0 38.0 41.0 34.0 41.0 56-57 37.61225162014047 40.0 37.5 41.0 34.0 41.0 58-59 37.42559714769536 40.0 37.0 41.0 33.0 41.0 60-61 37.21275177375908 40.0 36.5 41.0 33.0 41.0 62-63 36.971518394935586 39.5 36.0 41.0 33.0 41.0 64-65 36.707254370985375 39.0 35.5 41.0 32.5 41.0 66-67 36.41276749287281 39.0 35.0 41.0 32.0 41.0 68-69 36.08698457383339 38.5 35.0 41.0 32.0 41.0 70-71 35.73100880984874 37.0 35.0 40.0 32.0 41.0 72-73 35.373326093014285 37.0 35.0 39.0 31.0 41.0 74-75 35.00991375922605 36.0 35.0 39.0 31.0 41.0 76-77 34.63578170438063 36.0 35.0 38.5 31.0 40.5 78-79 34.284607415134644 35.0 35.0 37.0 31.0 39.0 80-81 34.019727487728375 35.0 35.0 37.0 31.0 39.0 82-83 33.80777238723322 35.0 35.0 36.5 31.0 39.0 84-85 33.56455554205935 35.0 35.0 36.0 31.0 37.0 86-87 33.37205784633852 35.0 35.0 36.0 31.0 37.0 88-89 33.201091763898916 35.0 35.0 35.5 31.0 37.0 90-91 33.06592953550019 35.0 35.0 35.0 31.0 36.0 92-93 32.938078123995226 35.0 34.5 35.0 30.0 36.0 94-95 32.85944325757197 35.0 34.0 35.0 30.0 36.0 96-97 32.799507705938254 35.0 34.0 35.0 30.0 36.0 98-99 32.730562244117834 35.0 34.0 35.0 30.0 36.0 100-101 32.67717656137242 35.0 34.0 35.0 30.0 35.0 102-103 31.85925962974342 34.5 32.5 35.0 28.0 35.0 104-105 32.53426623891624 35.0 34.0 35.0 29.5 35.0 106-107 32.60686425116286 35.0 34.0 35.0 30.0 35.0 108-109 32.59839164886358 35.0 34.0 35.0 30.0 35.0 110-111 32.57070171552691 35.0 34.0 35.0 30.0 35.0 112-113 32.51568195945897 35.0 34.0 35.0 30.0 35.0 114-115 32.47714869567081 35.0 34.0 35.0 30.0 35.0 116-117 32.4261365990983 35.0 34.0 35.0 29.5 35.0 118-119 32.356922483334166 35.0 34.0 35.0 29.0 35.0 120-121 32.272436534078324 35.0 34.0 35.0 29.0 35.0 122-123 32.13046292789929 35.0 34.0 35.0 29.0 35.0 124-125 30.185264759890536 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 4.822023564514666E-4 1101 2 0.012245707602971834 1101 3 -0.046901722918036626 1101 4 0.03167249109657888 1101 5 -0.055327100942598406 1101 6 0.04108836448492781 1101 7 0.0855725490197301 1101 8 0.07470974863895208 1101 9 0.016743755462528043 1101 10-11 0.0720844388841897 1101 12-13 0.0459784722378771 1101 14-15 0.111826439583119 1101 16-17 0.08428559339839126 1101 18-19 0.07763202014725579 1101 20-21 0.1884991059173302 1101 22-23 0.17675586102139107 1101 24-25 0.11647750080624064 1101 26-27 0.11123097881307586 1101 28-29 0.05685241440061617 1101 30-31 -0.03033614957695363 1101 32-33 -0.032647153011531316 1101 34-35 0.03139937899574363 1101 36-37 -0.02020953213268939 1101 38-39 -0.004848294516932583 1101 40-41 0.017548838918301612 1101 42-43 -0.02055968727378854 1101 44-45 -0.0011917456707521978 1101 46-47 -0.029402187385485945 1101 48-49 -0.025847831701589996 1101 50-51 -0.04047232148629121 1101 52-53 -0.024967882513870165 1101 54-55 -0.041044931010496555 1101 56-57 -0.06810372735341019 1101 58-59 -0.14323014162750525 1101 60-61 -0.2645448513715962 1101 62-63 -0.12433751271194637 1101 64-65 -0.16788552038092774 1101 66-67 -0.17052217426653016 1101 68-69 -0.21542700070987308 1101 70-71 -0.19128138572397546 1101 72-73 -0.3035607069004982 1101 74-75 -0.3927827667209698 1101 76-77 -0.3327483759348695 1101 78-79 -0.20982264885624602 1101 80-81 -0.2124605638015069 1101 82-83 -0.26389172019315055 1101 84-85 -0.1886615780497749 1101 86-87 -0.06464487151934861 1101 88-89 -0.13239068071816007 1101 90-91 -0.03953045778268205 1101 92-93 -0.021196628383925997 1101 94-95 -0.06995298740733347 1101 96-97 -0.1818383272171431 1101 98-99 -0.18574208296115557 1101 100-101 -0.1994458783280848 1101 102-103 -0.14870749375328884 1101 104-105 -0.1256745959002501 1101 106-107 -0.1780459156990588 1101 108-109 -0.2274622482988491 1101 110-111 -0.1671142633400251 1101 112-113 -0.18885620983656537 1101 114-115 -0.18503854245194873 1101 116-117 -0.26539970713091066 1101 118-119 -0.26362066460416855 1101 120-121 -0.38329368737259983 1101 122-123 -0.3558562029193624 1101 124-125 -0.21097524245221777 1102 1 0.0758089345113504 1102 2 0.15326681784875973 1102 3 0.18182278368423255 1102 4 0.0876396204565495 1102 5 -0.003348819823145277 1102 6 0.10953468791649357 1102 7 0.08299403186343568 1102 8 0.007406524183615204 1102 9 0.020664977618579883 1102 10-11 0.09735259812399022 1102 12-13 -0.024742050969869922 1102 14-15 0.020141284817547955 1102 16-17 0.15337327388913735 1102 18-19 0.036017436473066766 1102 20-21 0.09297944308790562 1102 22-23 0.15697904891501224 1102 24-25 0.08689163422159396 1102 26-27 0.1391561887266235 1102 28-29 0.03585506054385945 1102 30-31 0.036060121826160696 1102 32-33 -0.06521456640106038 1102 34-35 -0.00843347550129181 1102 36-37 -0.058923284006041854 1102 38-39 -0.10366854656589908 1102 40-41 -0.05773535424796705 1102 42-43 -0.042936831402428766 1102 44-45 0.0637843779584557 1102 46-47 0.0061184134831862025 1102 48-49 -0.043788922837556754 1102 50-51 0.10307331412255394 1102 52-53 0.08008760359768985 1102 54-55 -0.03133786103059322 1102 56-57 -0.0310533590093911 1102 58-59 -0.07980614571124534 1102 60-61 -0.11571838208583074 1102 62-63 -0.024447786859369103 1102 64-65 -0.05932438530982864 1102 66-67 -0.2224017431175156 1102 68-69 -0.17612206596904656 1102 70-71 -0.17459028640743668 1102 72-73 -0.22687545396803444 1102 74-75 -0.16322547761073736 1102 76-77 -0.1832341929997625 1102 78-79 -0.06581508346073406 1102 80-81 -0.22057187633655673 1102 82-83 -0.21484372094358406 1102 84-85 -0.1999796758807264 1102 86-87 -0.1468689189186918 1102 88-89 -0.2096703887479947 1102 90-91 -0.08859263669512529 1102 92-93 -0.05292853240724327 1102 94-95 -0.05665246363240328 1102 96-97 -0.021695941587033474 1102 98-99 -0.11946143034762713 1102 100-101 -0.12057313971163097 1102 102-103 -0.06955552485255723 1102 104-105 -0.02430254990578362 1102 106-107 -0.10590142711586736 1102 108-109 -0.07801896367811167 1102 110-111 -0.0886821850159265 1102 112-113 -0.0550083728896027 1102 114-115 -0.0767277329677114 1102 116-117 -0.005603741572215881 1102 118-119 0.009801411603888255 1102 120-121 -0.0950375023127279 1102 122-123 -0.10869649846716811 1102 124-125 -0.0715564341110877 1103 1 -0.0026684743085283458 1103 2 0.06892930280454124 1103 3 0.044833150181474934 1103 4 0.047371880970580094 1103 5 -0.15476398101043998 1103 6 0.021987387203466824 1103 7 -0.06549795054672813 1103 8 -0.04716935049619764 1103 9 0.008330305376297531 1103 10-11 0.021635499220288068 1103 12-13 0.06876688460809532 1103 14-15 0.03732659362325563 1103 16-17 0.1284395319030267 1103 18-19 0.05128639218754216 1103 20-21 0.03831155807755238 1103 22-23 -0.0034726900802297678 1103 24-25 0.07108965314399285 1103 26-27 0.07634803343856333 1103 28-29 -0.029230614822544965 1103 30-31 0.014981824207637828 1103 32-33 0.04335339327514731 1103 34-35 0.08581048513097755 1103 36-37 0.08654504857641143 1103 38-39 0.04267547054168119 1103 40-41 0.025990873772663292 1103 42-43 0.09532772685280122 1103 44-45 -0.02356885498842587 1103 46-47 -0.11870100302326136 1103 48-49 0.16753924099582918 1103 50-51 0.29336918550268365 1103 52-53 0.29599936251793224 1103 54-55 0.2887232465199645 1103 56-57 0.27974590406188327 1103 58-59 0.2805850991356422 1103 60-61 0.22053389966460202 1103 62-63 0.20882431201260943 1103 64-65 0.24502939546868419 1103 66-67 0.13442330822847737 1103 68-69 0.25126380420543626 1103 70-71 0.4158130361173349 1103 72-73 0.38330268649260546 1103 74-75 0.4146908797108537 1103 76-77 0.3623251980822033 1103 78-79 0.310721859215775 1103 80-81 0.22460212894716847 1103 82-83 0.33199429645588197 1103 84-85 0.30396037568736034 1103 86-87 0.30605510593044016 1103 88-89 0.310852674117271 1103 90-91 0.3403611106635438 1103 92-93 0.3222938258915633 1103 94-95 0.29048982241185684 1103 96-97 0.2563502877873205 1103 98-99 0.24678674795359257 1103 100-101 0.2770510900984462 1103 102-103 0.2690509522516784 1103 104-105 0.26078194095980933 1103 106-107 0.30668823007690804 1103 108-109 0.28520239202536146 1103 110-111 0.28540643679622946 1103 112-113 0.368678576238203 1103 114-115 0.33608877728138964 1103 116-117 0.3714149333521135 1103 118-119 0.3961062555776351 1103 120-121 0.3380667570252598 1103 122-123 0.39477186795815555 1103 124-125 0.14099694774447613 1104 1 0.07322079430785777 1104 2 0.13153251263667443 1104 3 0.16947048670979825 1104 4 0.048383019756748524 1104 5 0.010678648804031354 1104 6 0.10414063519465344 1104 7 0.06470115741545612 1104 8 0.0646199180185647 1104 9 0.06815923492075626 1104 10-11 0.03876032862237366 1104 12-13 0.05700109077268678 1104 14-15 0.046756097941681674 1104 16-17 0.12284117655328686 1104 18-19 0.15168332767485282 1104 20-21 0.17013052053737 1104 22-23 0.21402021416442096 1104 24-25 0.17141189646972066 1104 26-27 0.2301818753899525 1104 28-29 0.22824800137051682 1104 30-31 0.18160479341922553 1104 32-33 0.18151204349124583 1104 34-35 0.2391275792705443 1104 36-37 0.21699109797079075 1104 38-39 0.09215064378176407 1104 40-41 0.11645527609817918 1104 42-43 0.13240704992841046 1104 44-45 0.07943519261537801 1104 46-47 0.042462832962911534 1104 48-49 0.0804685049782492 1104 50-51 0.10413024273719884 1104 52-53 0.06502833393065544 1104 54-55 0.0361616102433544 1104 56-57 0.11822537489587148 1104 58-59 -0.028478362779992494 1104 60-61 -0.126600715934984 1104 62-63 0.02588745686763616 1104 64-65 0.025256601910705 1104 66-67 0.05011465785994318 1104 68-69 0.041007401766101736 1104 70-71 0.10270349472720142 1104 72-73 0.10374008785576905 1104 74-75 0.11788455394531638 1104 76-77 0.07535943757881114 1104 78-79 0.08242399703754444 1104 80-81 0.1412751469881144 1104 82-83 0.2255960744342005 1104 84-85 0.23439809207868478 1104 86-87 0.18878993656215926 1104 88-89 0.19974732158550523 1104 90-91 0.1645189946498533 1104 92-93 0.15386198528361916 1104 94-95 0.23714437573748626 1104 96-97 0.25211976440359507 1104 98-99 0.32516524717973994 1104 100-101 0.30647522877587363 1104 102-103 0.10616055867987484 1104 104-105 0.3307715450303803 1104 106-107 0.2683701349607617 1104 108-109 0.2566567754390334 1104 110-111 0.06108758358004707 1104 112-113 0.05743176589836452 1104 114-115 0.1900569635016467 1104 116-117 0.11712928003959178 1104 118-119 0.06590186327204606 1104 120-121 0.13774644451732598 1104 122-123 0.08863715175280618 1104 124-125 0.10399841414977118 1105 1 -0.06997532712257026 1105 2 -0.040833282142450855 1105 3 -0.10217109133093771 1105 4 0.0048958448050413494 1105 5 -0.08778909912914656 1105 6 -0.033626373245496666 1105 7 -0.005889371686883749 1105 8 0.006036351688308628 1105 9 -0.03434064950205595 1105 10-11 -0.0992755224177202 1105 12-13 0.025176157053692805 1105 14-15 0.004402081985993789 1105 16-17 -0.04033773895073978 1105 18-19 -0.06869825796383111 1105 20-21 0.014830451995067051 1105 22-23 0.09179230800103255 1105 24-25 0.0076862505942116854 1105 26-27 0.14264019222422064 1105 28-29 0.1177401250361001 1105 30-31 -0.038439608323074026 1105 32-33 0.01326180178416081 1105 34-35 0.09447066262883652 1105 36-37 0.15854923932873533 1105 38-39 0.22013982173646696 1105 40-41 0.0866745511578273 1105 42-43 0.00305808117847306 1105 44-45 -0.005568314901168492 1105 46-47 0.0860067914918119 1105 48-49 -0.0029922982731847014 1105 50-51 -0.006719340079385461 1105 52-53 0.05205554783690047 1105 54-55 0.0754623782789281 1105 56-57 0.14909503799337642 1105 58-59 0.08389181388226064 1105 60-61 0.20199428751223536 1105 62-63 0.2183164472455843 1105 64-65 0.16734921240753664 1105 66-67 0.057708581916685375 1105 68-69 0.10503314633447047 1105 70-71 0.24668755110474905 1105 72-73 0.009107995184962192 1105 74-75 0.07019438926288757 1105 76-77 0.00354228270738588 1105 78-79 -0.03714814624601104 1105 80-81 -0.03069316275071543 1105 82-83 0.12088514806792006 1105 84-85 0.008680860222796127 1105 86-87 -0.067246207943505 1105 88-89 0.025031792231914096 1105 90-91 0.09367168317635333 1105 92-93 -0.004529019393736178 1105 94-95 0.090285462323898 1105 96-97 0.09829456133949321 1105 98-99 -0.0741474202815624 1105 100-101 0.03899442966511657 1105 102-103 0.009039033784549844 1105 104-105 0.15608013613435645 1105 106-107 0.1538090167363464 1105 108-109 0.07697996947002395 1105 110-111 0.0745241859723933 1105 112-113 -0.05130576935267328 1105 114-115 0.0186007109765427 1105 116-117 -0.08176650551346398 1105 118-119 -0.029179472920901617 1105 120-121 -0.18727521364353095 1105 122-123 -0.31784130164345825 1105 124-125 -0.2920454288019272 1106 1 0.024438352307299738 1106 2 0.043408179083748166 1106 3 0.0011558781899623227 1106 4 -0.04412527435636093 1106 5 -0.06190893643690032 1106 6 -0.0037045967536570856 1106 7 -0.04934116679584122 1106 8 -0.04181872730596581 1106 9 -0.023569600500700005 1106 10-11 0.05314272630852912 1106 12-13 0.07579613468153212 1106 14-15 -0.05933683569156756 1106 16-17 -0.09756659508509813 1106 18-19 -0.06280777878060917 1106 20-21 0.12099742104164335 1106 22-23 0.017059014985022714 1106 24-25 0.033079245657319234 1106 26-27 0.09336511346710097 1106 28-29 0.08241306065091436 1106 30-31 0.006285937443010425 1106 32-33 -0.05964535374977942 1106 34-35 0.1131421278472402 1106 36-37 0.009866271815795358 1106 38-39 -0.020310506928751693 1106 40-41 -0.09829795796297702 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-0.0031408529478866853 2216 9 -0.027027990901949295 2216 10-11 -0.056117345246434525 2216 12-13 -0.06283041585005833 2216 14-15 0.016101729389731645 2216 16-17 -0.036448720368944976 2216 18-19 -0.0062344498635127366 2216 20-21 -0.049292498899148995 2216 22-23 -0.1253302881746734 2216 24-25 -0.003257725103019027 2216 26-27 0.018650154660441842 2216 28-29 -0.19250062878622032 2216 30-31 -0.19956139804472883 2216 32-33 -0.10174506520245785 2216 34-35 -0.08649338346637592 2216 36-37 -0.08415629276419878 2216 38-39 -0.09635928843546537 2216 40-41 0.043551997427073275 2216 42-43 0.04024516336099282 2216 44-45 0.020518654802238245 2216 46-47 0.09123964752157576 2216 48-49 0.16820561949882062 2216 50-51 0.14152647075560765 2216 52-53 0.2823330468591507 2216 54-55 0.15583189189011648 2216 56-57 0.14740481943219663 2216 58-59 0.18617125930867218 2216 60-61 0.14895856402289098 2216 62-63 0.25723619293969335 2216 64-65 0.3147711364371375 2216 66-67 0.39738339296086167 2216 68-69 0.37326931610214586 2216 70-71 0.2738295810041862 2216 72-73 0.35191008954009106 2216 74-75 0.26780519332183417 2216 76-77 0.2879962132613798 2216 78-79 0.3458697806819657 2216 80-81 0.24496556772081846 2216 82-83 0.3190234822248925 2216 84-85 0.2942436651063929 2216 86-87 0.35231227361870765 2216 88-89 0.3423479676993395 2216 90-91 0.3166933695142333 2216 92-93 0.289926760547381 2216 94-95 0.26914999926464844 2216 96-97 0.26482429251137063 2216 98-99 0.259685904461719 2216 100-101 0.3421051897632239 2216 102-103 0.47199520330967815 2216 104-105 0.3454020477336499 2216 106-107 0.3607311817926302 2216 108-109 0.3488110749487561 2216 110-111 0.3259604927766233 2216 112-113 0.41520819655190166 2216 114-115 0.3622691370874591 2216 116-117 0.2787419546746861 2216 118-119 0.39900973454091826 2216 120-121 0.32967169075527636 2216 122-123 0.37472916455368477 2216 124-125 0.44301470659745945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68.0 3 3.0 4 3.0 5 21.0 6 70.0 7 137.0 8 255.0 9 476.0 10 890.0 11 1493.0 12 1982.0 13 3361.0 14 5827.0 15 6368.0 16 2323.0 17 1591.0 18 1432.0 19 1187.0 20 1152.0 21 1098.0 22 1145.0 23 1218.0 24 1481.0 25 1987.0 26 2499.0 27 3126.0 28 4015.0 29 5383.0 30 6761.0 31 8628.0 32 11305.0 33 15007.0 34 21988.0 35 37335.0 36 87894.0 37 271018.0 38 187522.0 39 1736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42578528082597 18.81386793504515 14.318279914132912 22.44206686999597 2 14.777824617561109 19.91454518173439 41.65865230034939 23.64897790035511 3 19.738491107983165 25.56542366584022 28.30369327722086 26.39239194895575 4 12.121866001700523 16.692269768571776 37.844766606886395 33.3410976228413 5 13.09708608684082 38.25914898913667 33.757155304260486 14.886609619762018 6 31.04467800824539 36.51793050722722 18.23317161699665 14.204219867530743 7 28.606643469065503 32.79693048579206 20.39783647834692 18.198589566795516 8 26.348807133619612 35.355287695506476 19.36537650849904 18.930528662374872 9 26.860964439077716 16.92677036518359 20.317668998335204 35.89459619740349 10-11 25.024900505155156 26.97221289396029 26.805304486377956 21.1975821145066 12-13 26.255206956422327 24.086397965089276 27.846695770843905 21.811699307644492 14-15 22.587223218559988 25.458033538872655 26.73249640961152 25.222246832955836 16-17 21.621783833606038 28.886014990318454 27.26873253927992 22.223468636795587 18-19 21.468593925277048 27.12661746107733 30.14733096593954 21.257457647706083 20-21 22.555968162938075 26.076294336338453 30.183401083671967 21.184336417051504 22-23 21.95220239388154 26.223663301818846 30.04827203822894 21.77586226607067 24-25 21.655294126053 26.261780403981223 30.53452131726173 21.548404152704045 26-27 21.421079331508963 26.6764077538101 30.29644819480269 21.606064719878248 28-29 21.174146344948806 26.855677100823826 30.167337110683995 21.802839443543373 30-31 21.577055809998786 26.523360746515003 30.172267196353165 21.727316247133047 32-33 21.206799231192438 26.545438956250848 30.30780882699686 21.939952985559852 34-35 21.89186678765621 26.67590760019149 30.023578670591682 21.408646941560622 36-37 21.732317783319164 26.34459155311989 30.096029494773397 21.827061168787555 38-39 21.541616353594318 26.769579227905716 29.968919025129146 21.719885393370824 40-41 22.048057617696866 26.489564652000258 29.878534121194367 21.58384360910851 42-43 21.61599634173353 26.58638010246004 29.769500632337078 22.02812292346935 44-45 21.884364483377038 26.734782826153747 29.51156426616747 21.86928842430175 46-47 22.19060139900112 27.042162950048944 28.806419114442296 21.96081653650764 48-49 22.22032481405003 27.063097951513683 28.631150996377457 22.085426238058833 50-51 22.205320205491685 27.43499789220975 28.49625242038626 21.863429481912302 52-53 22.35558064262595 27.454289531784763 28.21202226398108 21.97810756160821 54-55 22.088641511321335 27.23772301492601 28.55084061533185 22.1227948584208 56-57 22.163164400494438 27.317819044420787 28.327557749880324 22.191458805204455 58-59 22.320784240873984 27.12133012282344 28.409011339197036 22.14887429710554 60-61 22.04534249805297 27.23972362940046 28.706531291753894 22.008402580792673 62-63 21.920804247018726 27.21750251863072 28.830640839686478 22.031052394664073 64-65 21.78397650707003 27.325892952835513 28.659588302121364 22.23054223797309 66-67 21.88679380095315 27.38719749637389 28.380645483970074 22.34536321870289 68-69 22.002686539437114 26.962281272105002 28.75068771122559 22.284344477232292 70-71 22.07077888208521 27.074029880606187 28.49818158434376 22.35700965296484 72-73 22.137656565945253 27.066456125810067 28.52661889008767 22.269268418157008 74-75 21.924162421315117 27.473152468258107 28.365712325928676 22.236972784498096 76-77 21.910729724129556 27.087748379859526 28.51768757546961 22.48383432054131 78-79 22.031123845181018 27.16448623505791 28.45138149574512 22.353008424015947 80-81 22.069135520195488 27.351114985316922 28.190587108897734 22.38916238558986 82-83 22.06313367677215 27.431711168430304 28.27375551062112 22.231399644176424 84-85 22.078424087398272 27.339039847953302 28.21602349292997 22.366512571718456 86-87 21.946383532084855 27.339397100538022 28.27254085183306 22.441678515544062 88-89 21.98975399587016 27.325035546632183 28.099344798759617 22.58586565873804 90-91 22.038274995784377 27.3341688364024 28.21151851015608 22.416037657657142 92-93 22.004972955979337 27.285594861278824 28.29333295226391 22.416099230477933 94-95 21.824383754071807 27.335389689495937 28.279684444358704 22.560542112073552 96-97 21.884364483377038 27.16905906814236 28.365640875411735 22.58093557306887 98-99 21.8962591413638 27.057507975806423 28.09391623706276 22.952316645767016 100-101 21.702324072625213 27.038824095132146 28.334222892904886 22.924628939337754 102-103 21.85309139131901 26.802489918288842 28.267231795044744 23.077186895347406 104-105 21.777344936218192 26.937051324830996 28.171602465672574 23.114001273278234 106-107 21.779017920942064 26.99967130648527 28.176179723897448 23.04513104867522 108-109 21.692954596606803 26.960805678907374 27.99284063262514 23.353399091860684 110-111 21.516388757095413 27.264243699160268 28.037307716492222 23.182059827252097 112-113 21.640557618666175 27.049678766887876 28.08772868789261 23.22203492655334 114-115 21.473801997290455 27.020941824771207 27.878679181648252 23.626576996290094 116-117 21.502509040476898 26.929224551798843 27.986644173545717 23.58162223417854 118-119 21.369180610860358 26.911251837175353 28.13491601330983 23.584651538654455 120-121 21.253582383568514 27.276774007775316 27.978638955040775 23.491004653615395 122-123 21.238521359631196 27.231993575105246 27.958009540168455 23.5714755250951 124-125 21.176720248136725 27.05574859365611 27.94717734473413 23.820353813473037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97.0 1 63.0 2 80.5 3 132.5 4 84.0 5 53.5 6 155.5 7 406.0 8 693.5 9 757.0 10 563.0 11 388.0 12 380.0 13 448.0 14 538.0 15 630.0 16 706.0 17 812.0 18 945.0 19 1063.5 20 1208.0 21 1401.0 22 1651.0 23 2006.0 24 2461.0 25 3088.0 26 3822.0 27 4885.5 28 6049.0 29 7278.0 30 8688.0 31 10338.5 32 12325.5 33 13904.5 34 15352.5 35 16985.0 36 18851.0 37 20359.0 38 21296.0 39 22093.5 40 22522.5 41 23112.0 42 23837.0 43 24302.0 44 24864.5 45 25811.0 46 26300.0 47 27205.5 48 28830.5 49 27802.5 50 26030.5 51 24799.5 52 23058.5 53 22109.0 54 21667.0 55 19937.5 56 17448.5 57 15670.5 58 14379.5 59 12757.0 60 10441.0 61 8510.5 62 6797.0 63 5011.0 64 3672.0 65 2825.5 66 2038.5 67 1477.0 68 1060.0 69 698.0 70 494.5 71 359.5 72 271.0 73 233.0 74 171.0 75 117.0 76 77.5 77 40.5 78 19.5 79 12.0 80 5.0 81 2.0 82 3.0 83 2.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 3.0 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014147202355009039 2 0.0 3 0.0 4 0.0 5 0.11832205606007559 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004930085669169817 22-23 1.4290103388898018E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0010003072372228613 92-93 0.0 94-95 5.001536186114307E-4 96-97 0.0 98-99 0.0017862629236122523 100-101 7.145051694449009E-5 102-103 4.2870310166694055E-4 104-105 0.002357867059168173 106-107 0.006430546525004109 108-109 3.572525847224505E-4 110-111 0.005287338253892267 112-113 0.008502611516394322 114-115 0.005287338253892267 116-117 0.002214966025279193 118-119 2.1435155083347028E-4 120-121 9.288567202783712E-4 122-123 0.0010003072372228613 124-125 0.0013575598219453119 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 699785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.34097724573182 #Duplication Level Percentage of deduplicated Percentage of total 1 83.08977520633454 57.61526211935415 2 10.901405764317206 15.11828258100018 3 2.846523508985812 5.921421655980777 4 1.1402833709442974 3.1627345311333968 5 0.5685578799147645 1.9712179507025605 6 0.33123859822895846 1.378104486162139 7 0.21995455175677728 1.0676304497923286 8 0.15716572801707854 0.8718420136192895 9 0.10045253022877786 0.6268928951582886 >10 0.5933210846190016 7.27946889460808 >50 0.03132923665046746 1.4537866703177997 >100 0.018961996703846767 2.470190544319729 >500 4.1221731938416174E-4 0.23495679549772683 >1k 6.183259790762426E-4 0.8282084123536053 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.37340040155190524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.28322984916795874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1200 0.17148124066677622 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 945 0.1350414770250863 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.2870310166694055E-4 0.0 24-25 0.0 0.0 0.0 8.574062033338811E-4 0.0 26-27 0.0 0.0 0.0 9.288567202783713E-4 0.0 28-29 0.0 0.0 0.0 0.0019291639575012326 0.0 30-31 0.0 0.0 0.0 0.003929778431946955 0.0 32-33 0.0 0.0 0.0 0.009002765135005752 0.0 34-35 0.0 0.0 0.0 0.013932850804175568 0.0 36-37 0.0 0.0 0.0 0.024578977828904593 0.0 38-39 0.0 0.0 0.0 0.048014747386697344 0.0 40-41 0.0 0.0 0.0 0.07445143865615868 0.0 42-43 0.0 0.0 0.0 0.10274584336617676 0.0 44-45 0.0 0.0 0.0 0.13296941203369605 0.0 46-47 0.0 0.0 0.0 0.16740856120094028 0.0 48-49 0.0 0.0 0.0 0.2075637517237437 0.0 50-51 0.0 0.0 0.0 0.25343498360210637 0.0 52-53 0.0 0.0 0.0 0.29766285359074574 0.0 54-55 0.0 0.0 0.0 0.34853562165522267 0.0 56-57 0.0 0.0 0.0 0.4093400115749838 0.0 58-59 0.0 0.0 0.0 0.4755031902655816 0.0 60-61 0.0 0.0 0.0 0.5511692877097966 0.0 62-63 0.0 0.0 0.0 0.626763934637067 0.0 64-65 0.0 0.0 0.0 0.70271583414906 0.0 66-67 0.0 0.0 0.0 0.7962445608293975 0.0 68-69 0.0 0.0 0.0 0.9045635445172446 0.0 70-71 0.0 0.0 0.0 1.0271726315939895 0.0 72-73 0.0 0.0 0.0 1.1464235443743434 0.0 74-75 0.0 0.0 0.0 1.2623877333752511 0.0 76-77 0.0 0.0 0.0 1.3907843123244996 0.0 78-79 0.0 0.0 0.0 1.5448316268568203 0.0 80-81 0.0 0.0 0.0 1.7221718099130447 0.0 82-83 0.0 0.0 0.0 1.9130875911887224 0.0 84-85 0.0 0.0 0.0 2.096501068185228 0.0 86-87 0.0 0.0 0.0 2.28334416999507 0.0 88-89 0.0 0.0 0.0 2.5008395435740978 0.0 90-91 0.0 0.0 0.0 2.7370549525925822 0.0 92-93 0.0 0.0 0.0 2.998135141507749 0.0 94-95 0.0 0.0 0.0 3.2480690497795752 0.0 96-97 0.0 0.0 0.0 3.5101495459319647 0.0 98-99 0.0 0.0 0.0 3.797523525082704 0.0 100-101 0.0 0.0 0.0 4.119193752366798 0.0 102-103 0.0 0.0 0.0 4.456868895446458 0.0 104-105 0.0 0.0 0.0 4.785827075458891 0.0 106-107 0.0 0.0 0.0 5.102995920175482 0.0 108-109 0.0 0.0 0.0 5.4542466614745955 0.0 110-111 0.0 0.0 0.0 5.855584215151797 0.0 112-113 0.0 0.0 0.0 6.289503204555685 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 220 0.0 37.860363 6 GTATCAA 5175 0.0 35.33999 1 CGCGGAA 60 9.2966366E-7 34.705334 82-83 AGGACCG 260 0.0 34.363243 5 TCGCGGA 65 1.7376078E-6 32.03569 82-83 ATCAACG 5960 0.0 30.580978 3 CAACGCA 6015 0.0 30.301352 5 TCAACGC 6020 0.0 30.276182 4 TATCAAC 6105 0.0 30.049778 2 AACGCAG 6105 0.0 30.015423 6 TAGAGCG 80 0.0053331424 29.781477 4 ATTCGCG 70 3.097126E-6 29.747427 80-81 TACCTGG 885 0.0 29.613216 2 GTACATG 4315 0.0 29.129969 1 GGTATCA 3620 0.0 29.127491 1 GTACCTG 920 0.0 28.490705 1 TACATGG 4360 0.0 28.142128 2 ACATGGG 4170 0.0 27.710342 3 ACCGAGT 280 3.6379788E-12 27.62261 8 GTGTAGC 525 0.0 27.232674 1 >>END_MODULE