FastQCFastQC Report
Fri 27 May 2016
SRR939953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544586
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51400.943836235231901No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28050.5150701633901716No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18920.34741987491415494No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15900.2919649054511133No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14160.2600140290055198No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13340.24495671941621708No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC11540.21190408861043067No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10200.18729824123278968No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9860.18105496652503003No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9310.17095555155659528No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8760.16085613658816056No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8600.15791812496097954No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA8260.1516748502532199No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA7470.13716841784401362No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7450.136801166390616No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6720.1233964883416026No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA6260.1149497049134572No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5920.10870643020569754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG101.8158062E-74910.1108-109
CCGCTCG202.910383E-103682.575108-109
CGTCGCT108.6395175E-43068.8125110-111
CGCCGTC108.6395175E-43068.8125110-111
TCGTTCG100.00135163922455.05108-109
CATCGCG100.00135163922455.05108-109
CCGCTCC1600.02148.1687108-109
CGGCGTC150.0019436832045.875110-111
CGCCGCC302.4991386E-62045.875110-111
TGCGTCG150.0019436832045.875110-111
CGGCGCC557.2759576E-121785.491108-109
GGGCTCG150.00304077941636.7001108-109
GCGCGTT150.00304077941636.7001108-109
CGTCGCC457.454219E-91636.7001108-109
CCGGTCG150.00304077941636.7001108-109
CCGCGTG150.00304077941636.7001108-109
CTGTGCG150.00304077941636.7001108-109
CTACGCT200.00345506641534.4062110-111
CGTCGTC200.00345506641534.4062110-111
CCTCGCG702.3646862E-111402.8857108-109
Produced by FastQC (version 0.11.5)