##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544586 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.840379664552522 34.0 31.0 34.0 30.0 34.0 2 31.851459640901528 34.0 31.0 34.0 30.0 34.0 3 32.034560198021985 34.0 31.0 34.0 30.0 34.0 4 35.257738906251724 37.0 35.0 37.0 33.0 37.0 5 35.34467283404274 37.0 35.0 37.0 33.0 37.0 6 35.38313875127161 37.0 37.0 37.0 33.0 37.0 7 35.33111574664057 37.0 37.0 37.0 33.0 37.0 8 35.35277440110469 37.0 37.0 37.0 33.0 37.0 9 36.98471499450959 39.0 39.0 39.0 34.0 39.0 10-11 36.95443143966242 39.0 38.0 39.0 34.0 39.0 12-13 36.89050489729813 39.0 38.0 39.0 34.0 39.0 14-15 38.38971806105923 41.0 39.0 41.0 34.0 41.0 16-17 38.41751807795279 41.0 39.0 41.0 34.0 41.0 18-19 38.437578270466005 41.0 39.0 41.0 34.0 41.0 20-21 38.49928202340861 41.0 39.0 41.0 34.0 41.0 22-23 38.48203681328569 41.0 39.0 41.0 34.0 41.0 24-25 38.49279452648434 41.0 39.0 41.0 34.0 41.0 26-27 38.45652385481816 41.0 39.0 41.0 34.0 41.0 28-29 38.40150224207012 41.0 39.0 41.0 34.0 41.0 30-31 38.31435622656477 41.0 39.0 41.0 33.5 41.0 32-33 38.21437752714907 40.0 39.0 41.0 33.0 41.0 34-35 38.13150631856126 40.0 38.0 41.0 33.0 41.0 36-37 38.28749178274873 41.0 39.0 41.0 33.0 41.0 38-39 38.231791672940545 41.0 39.0 41.0 33.0 41.0 40-41 38.157140470008414 41.0 39.0 41.0 33.0 41.0 42-43 38.09576907963113 41.0 38.0 41.0 33.0 41.0 44-45 38.00842383021231 41.0 38.0 41.0 33.0 41.0 46-47 37.86427763475374 40.0 38.0 41.0 33.0 41.0 48-49 37.7454497544924 40.0 38.0 41.0 32.0 41.0 50-51 37.63448289159105 40.0 38.0 41.0 32.0 41.0 52-53 37.57244402169722 40.0 38.0 41.0 32.0 41.0 54-55 37.416614639377435 40.0 38.0 41.0 31.5 41.0 56-57 37.16832786740754 40.0 37.5 41.0 30.5 41.0 58-59 36.98998505286585 40.0 37.0 41.0 30.5 41.0 60-61 36.75950446761394 40.0 36.5 41.0 30.0 41.0 62-63 36.54707061878197 40.0 36.0 41.0 30.0 41.0 64-65 36.27386308131314 39.0 36.0 41.0 30.0 41.0 66-67 35.92545070934618 39.0 35.0 41.0 29.0 41.0 68-69 35.45751176122779 39.0 35.0 40.5 28.5 41.0 70-71 34.98960953825475 37.5 34.5 40.0 28.0 41.0 72-73 34.59860701523726 37.0 34.0 39.5 27.5 41.0 74-75 34.18509932315557 37.0 34.0 39.0 26.5 41.0 76-77 33.69712956264024 36.0 34.0 39.0 26.0 40.5 78-79 33.16576077974828 36.0 34.0 38.5 26.0 40.0 80-81 32.63414961089708 35.0 33.0 37.0 24.0 39.0 82-83 32.38683880966459 35.0 33.0 37.0 23.5 39.0 84-85 31.900029563741995 35.0 33.0 37.0 19.5 39.0 86-87 31.19257748087538 35.0 34.0 36.0 2.0 37.0 88-89 15.672576966723346 18.0 16.0 18.5 2.0 19.5 90-91 2.7547357074915624 2.0 2.0 2.0 2.0 2.0 92-93 2.2943547942840983 2.0 2.0 2.0 2.0 2.0 94-95 2.1004304187033824 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19798485758147066 1101 2 0.11825986241324671 1101 3 0.1340031622461595 1101 4 0.2761450577713447 1101 5 0.31151938007111823 1101 6 0.1437854225606472 1101 7 0.21396752104599415 1101 8 0.25164482774184904 1101 9 0.3894202256670951 1101 10-11 0.21212835679646247 1101 12-13 0.34816170600822716 1101 14-15 0.35967966664295403 1101 16-17 0.17210002851587802 1101 18-19 0.11490755089433691 1101 20-21 0.20030947062357285 1101 22-23 0.3815442310489203 1101 24-25 0.37368453147961134 1101 26-27 0.23658626281424233 1101 28-29 0.3200508885354978 1101 30-31 0.19974537603471987 1101 32-33 0.30443389945119037 1101 34-35 0.34662745584554955 1101 36-37 0.3302744443756467 1101 38-39 0.3695011942565003 1101 40-41 0.3168214281297068 1101 42-43 0.1562873593304701 1101 44-45 0.21266812778201682 1101 46-47 0.32741215826946757 1101 48-49 0.3518008529055976 1101 50-51 0.20029174958293083 1101 52-53 -0.06429871743606697 1101 54-55 0.05251606389531105 1101 56-57 0.18498851349490764 1101 58-59 0.06727923047785112 1101 60-61 0.09196408955134672 1101 62-63 -0.03242465799981176 1101 64-65 -0.07979513325113885 1101 66-67 -0.1457062905820692 1101 68-69 0.06448305653929509 1101 70-71 -0.028159217368106226 1101 72-73 -0.1525094161504299 1101 74-75 -0.047466557361644846 1101 76-77 -0.027389868165819564 1101 78-79 -0.013487429312810661 1101 80-81 0.07594785754754696 1101 82-83 0.001926373189959918 1101 84-85 0.1355472402960416 1101 86-87 0.028521327161787013 1101 88-89 0.7371760445533617 1101 90-91 -0.04033571933472846 1101 92-93 -0.22847592575713627 1101 94-95 -0.04594134406663253 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.018287754501667308 1102 2 0.006037430507856101 1102 3 -0.02249896304252985 1102 4 -0.12973925103804618 1102 5 0.047228723253574856 1102 6 -0.12307714809509207 1102 7 -0.07450559607404017 1102 8 -0.020390327139125475 1102 9 0.06742236443541572 1102 10-11 0.08147657164382593 1102 12-13 -0.033733350561291786 1102 14-15 -0.12478281055400231 1102 16-17 -0.1094867937956181 1102 18-19 -0.20684767695637873 1102 20-21 -0.1924268048641622 1102 22-23 -0.17261423550811372 1102 24-25 -0.22754750691037628 1102 26-27 -0.09486171928480758 1102 28-29 -0.16678490258411927 1102 30-31 -0.21617317104065137 1102 32-33 -0.13002992570900318 1102 34-35 0.05279483693273335 1102 36-37 0.07627863204807284 1102 38-39 -0.03273193149049547 1102 40-41 -0.10391473952097385 1102 42-43 -0.027785107172654477 1102 44-45 -0.025700297527343707 1102 46-47 -0.10741022510924125 1102 48-49 -0.07534532921322068 1102 50-51 -0.0896066139897087 1102 52-53 -0.14509743058050617 1102 54-55 0.06635874760023341 1102 56-57 0.014629326226859973 1102 58-59 0.20806949638605943 1102 60-61 0.15161775286608048 1102 62-63 0.18498743233170245 1102 64-65 0.23987344752330841 1102 66-67 -0.026236309723145723 1102 68-69 0.0875970313233978 1102 70-71 0.2500226498305551 1102 72-73 0.15535549996132403 1102 74-75 0.0014555213773803644 1102 76-77 -0.07374991813032494 1102 78-79 0.04853775064027843 1102 80-81 -0.01698342328711533 1102 82-83 0.020704399592069933 1102 84-85 -0.1523956779544875 1102 86-87 -0.007990503462142584 1102 88-89 0.7770191416336729 1102 90-91 -0.01601396949873024 1102 92-93 -0.1364360979369721 1102 94-95 0.015727074952230247 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.2084690512589482 1103 2 0.18940832179699996 1103 3 0.28598395456308623 1103 4 0.139743830613817 1103 5 0.21846882651636435 1103 6 0.1463731243080133 1103 7 0.19630712355365887 1103 8 0.20375900674678604 1103 9 0.1272353517175162 1103 10-11 0.1932274631056501 1103 12-13 0.2889913712076435 1103 14-15 0.0908654742993491 1103 16-17 0.13515191593750586 1103 18-19 0.11062583820926619 1103 20-21 -0.014309710382157448 1103 22-23 0.19759064961634465 1103 24-25 0.21105948145960696 1103 26-27 0.2511921051511763 1103 28-29 0.09954935458858927 1103 30-31 0.10916247620811959 1103 32-33 0.190668393248707 1103 34-35 0.2875104757201683 1103 36-37 0.1706639335046347 1103 38-39 0.12608382730972068 1103 40-41 0.15063495353987122 1103 42-43 0.06963936680012495 1103 44-45 0.1583397297561433 1103 46-47 0.15790435514822576 1103 48-49 0.10578244554265126 1103 50-51 0.11641820016316018 1103 52-53 0.12691109982417004 1103 54-55 -0.015911307053073642 1103 56-57 0.06818179080583064 1103 58-59 0.07650958928804386 1103 60-61 0.032233530661123666 1103 62-63 -0.17238730972717065 1103 64-65 -1.6555153230513042E-5 1103 66-67 0.022574355009510327 1103 68-69 0.039166263155273384 1103 70-71 -0.36568089270490844 1103 72-73 -0.4410648383192992 1103 74-75 -0.2061700387542018 1103 76-77 -0.13557981080953851 1103 78-79 -0.3753655138799701 1103 80-81 -0.22746684166547482 1103 82-83 -0.3156940082959636 1103 84-85 -0.2315462637722554 1103 86-87 -0.4045852488019328 1103 88-89 0.42445095451734716 1103 90-91 -0.5964648376487203 1103 92-93 -0.3114691230360478 1103 94-95 -0.1058052896448638 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.2825053537702438 1104 2 -0.313764855871014 1104 3 -0.17267492883760482 1104 4 -0.26213712533090217 1104 5 -0.31394149130032645 1104 6 -0.2652917961130825 1104 7 -0.2826830475784021 1104 8 -0.3590110376577442 1104 9 -0.43115411093777567 1104 10-11 -0.5706077910978138 1104 12-13 -0.5464324675390486 1104 14-15 -0.5896115002054714 1104 16-17 -0.6686773511382782 1104 18-19 -0.3931310844071092 1104 20-21 -0.5583575076181049 1104 22-23 -0.709272095481694 1104 24-25 -0.5254170939033642 1104 26-27 -0.3646288797313275 1104 28-29 -0.3320003136247536 1104 30-31 -0.3538844728513979 1104 32-33 -0.3977315468800171 1104 34-35 -0.43032118620668314 1104 36-37 -0.5072236082805333 1104 38-39 -0.5352243600873763 1104 40-41 -0.4722059385421602 1104 42-43 -0.5331346690228642 1104 44-45 -0.6229604748040529 1104 46-47 -0.4386754981671501 1104 48-49 -0.5259942345950535 1104 50-51 -0.6359392313590604 1104 52-53 -0.4973542297173168 1104 54-55 -0.5783869843468921 1104 56-57 -0.4339137038805987 1104 58-59 -0.7021812254602509 1104 60-61 -0.6455417524801206 1104 62-63 -0.8663577732306393 1104 64-65 -0.702311620762174 1104 66-67 -0.7042953750708207 1104 68-69 -0.7005608236081855 1104 70-71 -0.4863614295506906 1104 72-73 -0.6375174651715625 1104 74-75 -0.6526750529736347 1104 76-77 -0.5294904030362559 1104 78-79 -0.6021928351392631 1104 80-81 -0.6546384227661015 1104 82-83 -0.6849251613169685 1104 84-85 -0.49836510326734285 1104 86-87 -0.8165869451547749 1104 88-89 -0.15105420938027514 1104 90-91 -0.6552160226010733 1104 92-93 -0.3114691230360478 1104 94-95 -0.1058052896448638 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.1349194599244221 1105 2 -0.2411562658471027 1105 3 -0.09417172579951227 1105 4 0.056037718353167065 1105 5 0.12055606006975239 1105 6 -0.07046268874417905 1105 7 0.11027730123947777 1105 8 -0.10621077681152968 1105 9 -0.05718183224149698 1105 10-11 -0.06620121623878816 1105 12-13 0.013061887186225363 1105 14-15 0.04432312201505084 1105 16-17 4.842967960527744E-4 1105 18-19 -0.0359427892417159 1105 20-21 0.13053543035420745 1105 22-23 -0.02271430653696882 1105 24-25 0.025489474562370162 1105 26-27 0.021346800195026105 1105 28-29 -0.068232364464194 1105 30-31 0.04762849913320366 1105 32-33 0.09830461356597908 1105 34-35 0.024487846232865706 1105 36-37 -0.08028772018867159 1105 38-39 0.10754259172001213 1105 40-41 0.03827905640714846 1105 42-43 -0.10667028603923967 1105 44-45 0.05522530140523685 1105 46-47 0.11191460246323004 1105 48-49 -0.011911415921531443 1105 50-51 0.07257151576076382 1105 52-53 0.12042934464303556 1105 54-55 0.06423452012202091 1105 56-57 0.1892897817408894 1105 58-59 0.09875890777590257 1105 60-61 0.08600401708014971 1105 62-63 0.11938199430935725 1105 64-65 0.09467586117678195 1105 66-67 0.1208689324719785 1105 68-69 0.2682766845432596 1105 70-71 0.21193923407276571 1105 72-73 0.4021832649421029 1105 74-75 0.30618093178476613 1105 76-77 0.5206761421579102 1105 78-79 0.6085795381777075 1105 80-81 0.37124321187017983 1105 82-83 0.2565011176606191 1105 84-85 0.099495382366932 1105 86-87 0.18447114427456057 1105 88-89 0.6719827310470521 1105 90-91 -0.771628236341531 1105 92-93 -0.3114691230360478 1105 94-95 -0.1058052896448638 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.012528900160429401 1106 2 -0.04805143822641256 1106 3 -0.05864583765231046 1106 4 -0.14337976708397093 1106 5 -0.12146874055026302 1106 6 -0.12306400377705273 1106 7 -0.16497958018256043 1106 8 0.08428773565128012 1106 9 0.19873081557469874 1106 10-11 -0.016237467145053586 1106 12-13 -0.0528460105112174 1106 14-15 0.19496751312482985 1106 16-17 0.048082361747667335 1106 18-19 0.1356990018030615 1106 20-21 0.04555518084796262 1106 22-23 -0.04571488155134773 1106 24-25 -0.1679059853241256 1106 26-27 -0.19967122525561365 1106 28-29 0.013182170899185053 1106 30-31 -0.06210861929475442 1106 32-33 0.01809010820334578 1106 34-35 0.009293716379616512 1106 36-37 0.06269397935381704 1106 38-39 -0.061771016120182765 1106 40-41 -0.005759544557875529 1106 42-43 -0.40497774372568074 1106 44-45 -0.26051696715148154 1106 46-47 -0.5159323437104248 1106 48-49 -0.0895789826106963 1106 50-51 -0.19694147206393353 1106 52-53 -0.2701647952104622 1106 54-55 -0.2708644823530406 1106 56-57 -0.3939560494048848 1106 58-59 -0.06202323677770494 1106 60-61 -0.10156317209958132 1106 62-63 -0.10495486411210919 1106 64-65 -0.2328792026998059 1106 66-67 -0.2458433603353356 1106 68-69 -0.1427867170417798 1106 70-71 -0.20312114243664325 1106 72-73 -0.10370750732720069 1106 74-75 -0.22587299456338883 1106 76-77 -0.3935717140384938 1106 78-79 -0.4234681380142007 1106 80-81 -0.4131170221356655 1106 82-83 -0.2755028074375119 1106 84-85 -0.27558586966436494 1106 86-87 -0.25450233006229794 1106 88-89 0.45323351231658293 1106 90-91 -0.10247400748580926 1106 92-93 -0.3114691230360478 1106 94-95 -0.1058052896448638 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.07177165054896406 1107 2 -0.037037250356526386 1107 3 0.007771770540024647 1107 4 0.17527302001334988 1107 5 0.1617355297144556 1107 6 0.1133360131492509 1107 7 0.11251357373195248 1107 8 0.019904306502979807 1107 9 -0.05409073886453086 1107 10-11 0.06435969890802795 1107 12-13 -0.028968587178304972 1107 14-15 -0.05156259453855938 1107 16-17 0.1020576251632761 1107 18-19 0.11457361416047007 1107 20-21 0.04027855667840896 1107 22-23 0.07596484254990088 1107 24-25 0.046794358642557654 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-0.1441676883012093 2216 34-35 -0.01817826763667796 2216 36-37 0.017131533135604116 2216 38-39 0.03051605745824304 2216 40-41 -0.07845521135312339 2216 42-43 -0.11986998233383162 2216 44-45 -0.23142040226220928 2216 46-47 -0.20166372655761933 2216 48-49 -0.19734779263611557 2216 50-51 -0.25379191171459325 2216 52-53 -0.17317336073274703 2216 54-55 -0.12402536778670736 2216 56-57 -0.18387449890688146 2216 58-59 -0.3353187868602987 2216 60-61 -0.32463766049260556 2216 62-63 -0.32234702853848063 2216 64-65 -0.2496826111051007 2216 66-67 -0.16773736941518536 2216 68-69 -0.2753297671985493 2216 70-71 -0.3599785057196314 2216 72-73 -0.2665043580635782 2216 74-75 -0.29235099224892025 2216 76-77 -0.3197324115449973 2216 78-79 -0.37898627298547183 2216 80-81 -0.4024125920000614 2216 82-83 -0.44908321553618435 2216 84-85 -0.4693066296813768 2216 86-87 -0.35184612797135983 2216 88-89 -1.1311507294962198 2216 90-91 -0.771628236341531 2216 92-93 -0.3114691230360478 2216 94-95 -0.1058052896448638 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6427.0 3 1044.0 4 759.0 5 721.0 6 725.0 7 679.0 8 805.0 9 913.0 10 1093.0 11 1269.0 12 1640.0 13 1861.0 14 2201.0 15 2548.0 16 2907.0 17 3419.0 18 4214.0 19 5403.0 20 5901.0 21 8348.0 22 10105.0 23 12060.0 24 19314.0 25 32695.0 26 54998.0 27 136645.0 28 217585.0 29 8007.0 30 300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.32790780519514 20.510993672257456 14.767364566845274 23.393733955702128 2 12.471881845909762 20.77245274772897 45.64588662393013 21.109778782431135 3 20.389747810240433 25.995679266357065 29.2459658456293 24.368607077773206 4 11.00772146154793 17.429780260762374 40.67922406935537 30.883274208334328 5 11.247349836305313 39.40026382218825 34.85135199685471 14.501034344651723 6 27.817277711876542 37.39960263392742 20.73354805301642 14.049571601179611 7 27.560201694498204 32.04727995211041 22.635910581616127 17.756607771775258 8 23.365822845243912 38.990352304319245 20.683785481081042 16.960039369355805 9 24.8375830447349 19.81578667097575 23.31771290484882 32.02891737944053 10-11 23.805645787694438 26.312426205004563 30.888129082251925 18.993798925049074 12-13 27.263220328401072 24.367583707017175 29.236766817609233 19.13242914697252 14-15 20.228136812309547 30.692058455467507 26.608218544195537 22.47158618802741 16-17 19.093070870479355 31.136553522406967 31.019032841521526 18.75134276559215 18-19 20.46467682978897 29.56915841597795 31.15854268796007 18.80762206627301 20-21 21.576903301626942 29.10288662822726 28.245895919791398 21.0743141503544 22-23 19.740021741365886 31.813182172079973 28.855603543255036 19.591192543299105 24-25 21.472733415842495 29.321356039266526 30.721594018208325 18.484316526682655 26-27 19.019860958599743 30.792106297260673 30.918165358639406 19.269867385500177 28-29 18.522755547149508 32.21235021755238 27.89729360986372 21.367600625434395 30-31 20.157330522635544 29.19841861524167 29.646189949796725 20.99806091232606 32-33 19.160672739859596 30.64247795085745 31.035809313249317 19.161039996033633 34-35 19.826161524534232 30.807163606849976 30.642818581454534 18.723856287161254 36-37 18.75758605176419 30.015975467557865 30.80510847709724 20.42133000358071 38-39 20.136672628381927 31.428094001682013 29.19245077912396 19.2427825908121 40-41 22.14939421872762 28.819782366788715 30.52125835037992 18.509565064103743 42-43 18.661882604400407 31.80590393436482 30.256378239616883 19.27583522161789 44-45 18.86851351810417 31.0919609353553 29.349347989155035 20.690177557385493 46-47 21.433437510328947 29.43171510101251 29.367721535257974 19.767125853400564 48-49 20.131109009612825 30.02708484442282 29.378242147690443 20.463563998273916 50-51 17.831008933408008 31.945885398973527 30.42996042858323 19.79314523903523 52-53 18.40462296129537 31.011355414939057 30.397770049174973 20.186251574590607 54-55 18.686378547812666 29.25822448003364 30.86552260142477 21.189874370728923 56-57 20.144843973220024 29.595692874954555 28.13504203192884 22.12442111989658 58-59 20.973914129265168 30.64079869846085 27.291465443474493 21.09382172879949 60-61 19.459185509726655 28.46933266738403 32.21740918789686 19.854072634992452 62-63 19.683576147752603 28.723011608818442 31.160184066428442 20.433228177000508 64-65 20.222058591296875 30.86399576926326 28.410572434840407 20.503373204599455 66-67 20.356564436103756 31.748979959088185 27.19891807721829 20.695537527589767 68-69 20.911190927386773 28.849543872019744 30.384109705757055 19.855155494836428 70-71 22.60460239521398 28.61439699147609 29.17105838196355 19.60994223134638 72-73 20.86070886875535 30.120403388996415 29.360009254736624 19.658878487511615 74-75 19.499124105283645 31.78111446126048 30.170074148068444 18.54968728538743 76-77 20.297712390696788 29.068319787875563 30.627026768958437 20.006941052469216 78-79 19.909527189127104 29.132906156723443 30.01936342177095 20.938203232378505 80-81 20.866493079146363 29.92447473860878 29.63269345888436 19.576338723360497 82-83 20.08812247168935 29.44932177464225 31.51420444633784 18.94835130733056 84-85 19.438894866926436 29.928514504596155 31.24731447374703 19.385276154730384 86-87 18.162144990501865 32.35674064715868 28.382305279017515 21.098809083321935 88-89 28.505076887025325 19.413433559099154 35.740693132153766 16.340796421721752 90-91 30.27970514405698 15.741406461853074 17.412099243952742 36.566789150137204 92-93 22.264286614202263 17.839638382801674 36.59837690173219 23.29769810126388 94-95 15.900239390931034 7.163964526314741 28.183716768322036 48.75207931443219 96-97 27.460664343423403 7.385321670959874 15.445780437534285 49.70823354808244 98-99 57.81071386337656 13.87782989738132 18.588303309813814 9.723152929428306 100-101 30.728635534109273 7.19045475866286 13.049885394056442 49.03102431317142 102-103 23.0245604700838 2.0575946544398973 37.51868051045717 37.39916436501913 104-105 41.20678657992091 4.461574909336103 42.34505130027518 11.986587210467807 106-107 5.720726574761824 1.9094080834941167 6.6334436324739325 85.73642170927013 108-109 18.009415410034908 11.343372373265618 22.783442416012292 47.86376980068718 110-111 40.16896548603969 2.813628233704422 26.195531855212085 30.82187442504381 112-113 27.177330923734672 2.772312030290642 39.48309523900493 30.567261806969753 114-115 28.987252765613796 4.017711067328511 24.21765676452774 42.77737940252995 116-117 19.85763297058695 4.310071675189022 20.54679646868581 55.28549888553822 118-119 20.763938114964656 4.382222684676365 15.996722356261664 58.85711684409731 120-121 25.746289607156893 4.223203758117947 30.711571933896053 39.318934700829104 122-123 34.326029518950435 1.712068756073858 34.35753765792031 29.604364067055393 124-125 38.780607310704 2.1180818715167353 20.54678883593582 38.554521981843436 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20.0 1 48.5 2 89.5 3 125.0 4 162.5 5 166.0 6 136.5 7 113.0 8 91.5 9 72.0 10 63.5 11 57.5 12 49.5 13 60.0 14 78.0 15 101.0 16 138.5 17 171.5 18 240.5 19 362.5 20 526.5 21 740.0 22 1027.5 23 1414.0 24 1993.5 25 2749.0 26 3626.0 27 4747.0 28 6262.0 29 8383.5 30 11071.0 31 14320.0 32 18498.5 33 23358.5 34 27814.5 35 30800.0 36 31799.0 37 31087.5 38 29506.5 39 27514.5 40 25459.5 41 23356.5 42 21650.0 43 20958.5 44 20621.5 45 19579.0 46 17570.0 47 15886.0 48 14954.5 49 14093.5 50 13233.0 51 11739.5 52 9567.0 53 7701.5 54 6287.5 55 5027.5 56 4030.5 57 3274.0 58 2558.0 59 1950.0 60 1469.0 61 1115.5 62 848.5 63 603.5 64 406.5 65 278.0 66 199.0 67 142.0 68 101.0 69 79.5 70 65.5 71 50.0 72 33.5 73 30.0 74 24.0 75 13.0 76 9.0 77 8.5 78 10.0 79 6.5 80 3.5 81 3.0 82 1.5 83 1.5 84 1.0 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0016526315402893206 3 0.04296842004752234 4 0.047559063214992675 5 0.05141520347566775 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.508771800964402E-4 12-13 0.0014690058135905073 14-15 9.18128633494067E-5 16-17 1.836257266988134E-4 18-19 0.006518713297807875 20-21 0.0011017543601928804 22-23 3.672514533976268E-4 24-25 0.0 26-27 0.0 28-29 8.263157701446603E-4 30-31 0.0 32-33 0.0012853800868916937 34-35 0.0 36-37 1.836257266988134E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.754385900482201E-4 46-47 0.0 48-49 1.836257266988134E-4 50-51 1.836257266988134E-4 52-53 0.0 54-55 0.0013771929502411006 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.672514533976268E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.5906431674703357E-4 80-81 0.0 82-83 5.508771800964402E-4 84-85 0.0 86-87 0.0010099414968434738 88-89 19.288964461076855 90-91 26.18998652187166 92-93 40.24323063758525 94-95 44.695420007124675 96-97 48.28245676532265 98-99 93.0123984090667 100-101 51.652539727425975 102-103 53.062693495609516 104-105 74.80976374714004 106-107 81.63173493259099 108-109 12.647497364970823 110-111 39.11944119018851 112-113 51.939454925392866 114-115 86.81209212135457 116-117 66.3876779792356 118-119 71.76405563125016 120-121 80.3773875935114 122-123 75.81428828504588 124-125 49.96869181359786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 544586.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.91992165580501 #Duplication Level Percentage of deduplicated Percentage of total 1 91.42163786155729 71.23566859817922 2 6.31422605002754 9.84007998270378 3 1.078320574081578 2.5206796395683773 4 0.38596844989252027 1.2029852550895073 5 0.2021272883977688 0.7874871238227225 6 0.12308538859264351 0.5754482301667859 7 0.07450707279004802 0.40639096922227325 8 0.05647596428516985 0.3520482170029181 9 0.04413753113999742 0.3095273671658328 >10 0.2509118581409011 3.7092351377501136 >50 0.022649465186547563 1.21847793214644 >100 0.02146979206934814 3.2925360529360943 >500 0.0025952495907964257 1.5435026633405624 >1k 0.0016515224668704526 2.061006236149481 >5k 2.3593178098149328E-4 0.9449265947558947 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5140 0.943836235231901 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2805 0.5150701633901716 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1892 0.34741987491415494 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1590 0.2919649054511133 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1416 0.2600140290055198 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1334 0.24495671941621708 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1154 0.21190408861043067 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1020 0.18729824123278968 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 986 0.18105496652503003 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 931 0.17095555155659528 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 876 0.16085613658816056 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 860 0.15791812496097954 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 826 0.1516748502532199 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 747 0.13716841784401362 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 745 0.136801166390616 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 672 0.1233964883416026 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 626 0.1149497049134572 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 592 0.10870643020569754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.18128633494067E-5 0.0 14-15 0.0 0.0 0.0 1.836257266988134E-4 0.0 16-17 0.0 0.0 0.0 1.836257266988134E-4 0.0 18-19 0.0 0.0 0.0 1.836257266988134E-4 0.0 20-21 0.0 0.0 0.0 1.836257266988134E-4 0.0 22-23 0.0 0.0 0.0 3.672514533976268E-4 0.0 24-25 0.0 0.0 0.0 5.508771800964402E-4 0.0 26-27 0.0 0.0 0.0 6.42690043445847E-4 0.0 28-29 0.0 0.0 0.0 9.18128633494067E-4 0.0 30-31 0.0 0.0 0.0 0.0029380116271810145 0.0 32-33 0.0 0.0 0.0 0.007712280521350163 0.0 34-35 9.18128633494067E-5 0.0 0.0 0.011752046508724058 0.0 36-37 1.836257266988134E-4 0.0 0.0 0.01928070130337541 0.0 38-39 1.836257266988134E-4 0.0 0.0 0.03635789388636505 0.0 40-41 1.836257266988134E-4 0.0 0.0 0.058117542500174445 0.0 42-43 1.836257266988134E-4 0.0 0.0 0.07840818530039333 0.0 44-45 1.836257266988134E-4 0.0 0.0 0.09704619656032289 0.0 46-47 1.836257266988134E-4 0.0 0.0 0.12238654684475914 0.0 48-49 1.836257266988134E-4 0.0 0.0 0.15286841747676216 0.0 50-51 1.836257266988134E-4 0.0 0.0 0.18849180845633196 0.0 52-53 1.836257266988134E-4 0.0 0.0 0.22420701229925116 0.0 54-55 1.836257266988134E-4 0.0 0.0 0.26451285930964075 0.0 56-57 1.836257266988134E-4 0.0 0.0 0.32033508022608004 0.0 58-59 1.836257266988134E-4 0.0 0.0 0.3751473596456758 0.0 60-61 1.836257266988134E-4 0.0 0.0 0.4395081768536099 0.0 62-63 1.836257266988134E-4 0.0 0.0 0.5059806899185804 0.0 64-65 1.836257266988134E-4 0.0 0.0 0.5712596357600086 0.0 66-67 1.836257266988134E-4 0.0 0.0 0.6444344878494856 0.0 68-69 1.836257266988134E-4 0.0 0.0 0.7391853628260734 0.0 70-71 1.836257266988134E-4 0.0 0.0 0.8507379917956026 0.0 72-73 1.836257266988134E-4 0.0 0.0 0.9527420829767934 0.0 74-75 1.836257266988134E-4 0.0 0.0 1.0590613787354064 0.0 76-77 1.836257266988134E-4 0.0 0.0 1.1715321363384295 0.0 78-79 1.836257266988134E-4 0.0 0.0 1.2024730712871796 0.0 80-81 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 82-83 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 84-85 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 86-87 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 88-89 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 90-91 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 92-93 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 94-95 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 96-97 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 98-99 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 100-101 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 102-103 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 104-105 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 106-107 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 108-109 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 110-111 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 112-113 1.836257266988134E-4 1.836257266988134E-4 0.0 1.2029321356039266 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGCG 10 1.8158062E-7 4910.1 108-109 CCGCTCG 20 2.910383E-10 3682.575 108-109 CGTCGCT 10 8.6395175E-4 3068.8125 110-111 CGCCGTC 10 8.6395175E-4 3068.8125 110-111 TCGTTCG 10 0.0013516392 2455.05 108-109 CATCGCG 10 0.0013516392 2455.05 108-109 CCGCTCC 160 0.0 2148.1687 108-109 CGGCGTC 15 0.001943683 2045.875 110-111 CGCCGCC 30 2.4991386E-6 2045.875 110-111 TGCGTCG 15 0.001943683 2045.875 110-111 CGGCGCC 55 7.2759576E-12 1785.491 108-109 GGGCTCG 15 0.0030407794 1636.7001 108-109 GCGCGTT 15 0.0030407794 1636.7001 108-109 CGTCGCC 45 7.454219E-9 1636.7001 108-109 CCGGTCG 15 0.0030407794 1636.7001 108-109 CCGCGTG 15 0.0030407794 1636.7001 108-109 CTGTGCG 15 0.0030407794 1636.7001 108-109 CTACGCT 20 0.0034550664 1534.4062 110-111 CGTCGTC 20 0.0034550664 1534.4062 110-111 CCTCGCG 70 2.3646862E-11 1402.8857 108-109 >>END_MODULE