FastQCFastQC Report
Fri 27 May 2016
SRR939953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544586
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57941.063927460492925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26960.4950549591800009No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18180.33383157113844275No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16480.3026151975996445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15680.28792513946373943No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13680.25119999412397676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.23908069616185504No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12080.2218198778521666No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11070.20327367945558644No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11020.2023555508220924No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10720.196846779021128No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10380.1906035043133683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.17921870925804187No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9650.17719882626435493No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8300.15240935316001514No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA8280.1520421017066175No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA7180.13184327176974803No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6750.12394736552169905No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA6280.11531695636685481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.10485028994502246No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5600.1028304069513355No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG152.67846E-4119.250111
TACCTGG7250.053.4225352
ATAACGC350.00774933451.0742953
GTACCTG8350.047.842861
ACCTGGG8000.047.669343
CCTGGGG5250.034.0495264
GACGAGG750.003910732531.7299567
TAGCCCT5250.030.6445754
CATGGGC5650.030.584314
TAGGACC13550.030.3430274
GTACATG27800.029.8125291
TACATGG28400.028.7442762
TACCGTG850.007210836327.997027
GTGTAGC4500.027.8250261
ACATGGG27950.027.0751613
TCGTAAT557.755799E-427.04257444-45
GTATAGC2705.820766E-1126.5000271
GTAGCCC4150.025.8448243
CATGGGG13250.025.1838044
AGTACTT21000.025.0723312-13
Produced by FastQC (version 0.11.5)