FastQCFastQC Report
Fri 27 May 2016
SRR939952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759127
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19110.2517365342031043No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17330.22828854723913128No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC16420.21630109322945965No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA10470.1379215862431451No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9490.12501202038657563No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9490.12501202038657563No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9220.1214553032628269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG109.3343295E-42955.3955110-111
CCGCTCA201.38E-62500.7192108-109
CTGCGCG251.6293907E-62364.3164110-111
GTCGTCG251.6293907E-62364.3164110-111
CCGCTCC1350.02222.8616108-109
GTCGGCT402.226443E-92216.5466110-111
CAGCGCG252.6949128E-62000.5754108-109
CGACGCC252.6949128E-62000.5754108-109
CGCATCC150.00209998921970.2635110-111
CGTCGCT150.00209998921970.2635110-111
CGCGTCT302.8152353E-61970.2635110-111
CCGCGCC1200.01875.5394108-109
CATCGCG304.65612E-61667.1461108-109
CCGTTCG150.00293492271667.1461108-109
CGGCTAG200.00373289731477.6978110-111
CGTCGCG200.00373289731477.6978110-111
CTCCGCC1450.01426.7426110-111
CCGCACC753.092282E-111333.7169108-109
CCGCTCG401.1033459E-51250.3596108-109
CGGCGCT250.00583199971182.1582110-111
Produced by FastQC (version 0.11.5)