FastQCFastQC Report
Fri 27 May 2016
SRR939952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759127
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20310.2675441658642098No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19360.255029790799168No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15950.21010977082885998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.2074751655520091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.16334552716475637No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA10480.13805331650698763No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9340.12303606642893745No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9220.1214553032628269No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9110.1200062703605589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.11078515189158072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA350.00775096151.0735361
TGTACGA558.480065E-443.320832
TAGGACC11950.038.3815354
TGTACCG650.001927939436.656095
GTGTAGC5050.034.2175831
TACCTGG8500.030.1334632
CACAGCG800.005363043429.747767
GTACATG27750.029.2024041
GTACCTG9100.028.810711
ACATGGG24550.028.1453043
TACATGG27200.028.0311262
ACCTGGG9100.027.4920673
CATGGGG11150.026.7112774
GTAGCCC4950.026.4738433
GAGTACT21500.026.01199512-13
TAGAACG1401.0475178E-425.5283474
GTACTTT23550.023.87414-15
GTAGGAC45650.023.6176823
GTATCAA48000.023.089491
GTATAGA4650.023.0654681
Produced by FastQC (version 0.11.5)