##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939952_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759127 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81536817950093 34.0 31.0 34.0 31.0 34.0 2 32.89649031058044 34.0 31.0 34.0 31.0 34.0 3 32.941129745088766 34.0 31.0 34.0 31.0 34.0 4 36.34432446744748 37.0 37.0 37.0 35.0 37.0 5 36.25282067427453 37.0 37.0 37.0 35.0 37.0 6 36.38999403261905 37.0 37.0 37.0 35.0 37.0 7 36.3890152767587 37.0 37.0 37.0 35.0 37.0 8 36.407331052643364 37.0 37.0 37.0 35.0 37.0 9 38.24964334031065 39.0 39.0 39.0 37.0 39.0 10-11 38.234247365724045 39.0 39.0 39.0 37.0 39.0 12-13 38.21874403097242 39.0 39.0 39.0 37.0 39.0 14-15 39.74926395715078 41.0 40.0 41.0 37.0 41.0 16-17 39.768569685968224 41.0 40.0 41.0 37.5 41.0 18-19 39.81180224125871 41.0 40.0 41.0 37.5 41.0 20-21 39.83502233486624 41.0 40.0 41.0 38.0 41.0 22-23 39.79195049049764 41.0 40.0 41.0 37.5 41.0 24-25 39.76948455265061 41.0 40.0 41.0 37.0 41.0 26-27 39.68678165840498 41.0 40.0 41.0 37.0 41.0 28-29 39.61160714873796 41.0 40.0 41.0 37.0 41.0 30-31 39.50819428106233 41.0 40.0 41.0 37.0 41.0 32-33 39.393226034642424 41.0 39.0 41.0 36.0 41.0 34-35 39.32978869148377 41.0 39.0 41.0 36.0 41.0 36-37 39.333031890579576 41.0 39.5 41.0 36.0 41.0 38-39 39.3975309796648 41.0 40.0 41.0 36.0 41.0 40-41 39.3291623140792 41.0 40.0 41.0 36.0 41.0 42-43 39.27244387302783 41.0 40.0 41.0 36.0 41.0 44-45 39.168114821367176 41.0 40.0 41.0 35.5 41.0 46-47 39.00802764227856 41.0 40.0 41.0 35.0 41.0 48-49 38.84047596778932 41.0 39.0 41.0 35.0 41.0 50-51 38.720723278186654 41.0 39.0 41.0 35.0 41.0 52-53 38.64400093791947 41.0 39.0 41.0 35.0 41.0 54-55 38.56700525735483 41.0 39.0 41.0 35.0 41.0 56-57 38.4399448313655 41.0 39.0 41.0 35.0 41.0 58-59 38.3124806521175 41.0 39.0 41.0 35.0 41.0 60-61 38.15007502038526 40.0 38.0 41.0 34.0 41.0 62-63 37.96650889772067 40.0 38.0 41.0 34.0 41.0 64-65 37.748651411423914 40.0 37.0 41.0 34.0 41.0 66-67 37.48931601695105 39.5 37.0 41.0 34.0 41.0 68-69 37.158373368356024 39.0 36.0 41.0 33.5 41.0 70-71 36.776649361701004 39.0 36.0 41.0 33.0 41.0 72-73 36.42558030474479 38.0 35.0 40.0 32.5 41.0 74-75 36.06491601537029 37.0 35.0 39.5 32.0 41.0 76-77 35.65052619653892 37.0 35.0 39.0 32.0 41.0 78-79 35.24666557769649 36.0 35.0 39.0 31.5 40.5 80-81 34.9227968442698 36.0 35.0 37.5 31.5 39.0 82-83 34.64160278846623 35.5 35.0 37.0 32.0 39.0 84-85 34.32985323931305 35.0 35.0 37.0 31.5 39.0 86-87 34.07498942864633 35.0 35.0 36.0 31.0 37.0 88-89 33.82365598905058 35.0 35.0 36.0 31.0 37.0 90-91 33.60899164434936 35.0 35.0 36.0 31.0 37.0 92-93 33.46290080579402 35.0 35.0 35.5 31.0 36.0 94-95 33.35401454565573 35.0 34.0 35.0 31.0 36.0 96-97 33.21442854752894 35.0 34.0 35.0 31.0 36.0 98-99 33.09455005552431 35.0 34.0 35.0 30.5 36.0 100-101 33.085348696594906 35.0 34.0 35.0 31.0 36.0 102-103 32.23754655018199 34.5 33.0 35.0 28.0 35.0 104-105 32.90832825074065 35.0 34.0 35.0 30.0 35.0 106-107 32.96666302212937 35.0 34.0 35.0 31.0 35.0 108-109 32.974642582861634 35.0 34.0 35.0 31.0 35.0 110-111 32.95989208656786 35.0 34.0 35.0 31.0 35.0 112-113 32.88483876874357 35.0 34.0 35.0 30.5 35.0 114-115 32.824587322015944 35.0 34.0 35.0 30.0 35.0 116-117 32.758095154038784 35.0 34.0 35.0 30.0 35.0 118-119 32.67267334714745 35.0 34.0 35.0 30.0 35.0 120-121 32.56839567028969 35.0 34.0 35.0 30.0 35.0 122-123 32.40111272553868 35.0 34.0 35.0 29.0 35.0 124-125 30.3470335003234 33.5 30.5 34.5 23.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09468942220942722 1101 2 0.10692437582116554 1101 3 0.1192131215973049 1101 4 0.1130537029330867 1101 5 0.0840778171075911 1101 6 0.06328359699312358 1101 7 0.06196231474795866 1101 8 0.09963234060870008 1101 9 -0.026326966445282096 1101 10-11 0.04098341565356378 1101 12-13 0.052452518205612364 1101 14-15 0.1687664667570914 1101 16-17 0.20075236652079553 1101 18-19 0.2074774779265809 1101 20-21 0.10661443334074505 1101 22-23 0.1203657431919396 1101 24-25 0.1916714721305084 1101 26-27 0.1762233997148428 1101 28-29 0.21976131967724655 1101 30-31 0.19217277080046813 1101 32-33 0.2265793087562855 1101 34-35 0.29233784120746265 1101 36-37 0.18166085604720195 1101 38-39 0.12689857653631265 1101 40-41 0.10076120842172998 1101 42-43 0.18219362411590367 1101 44-45 0.14599240211200737 1101 46-47 0.010972914715758009 1101 48-49 0.11854706458694864 1101 50-51 0.1126933538170718 1101 52-53 -0.008183686960464343 1101 54-55 0.084479923390262 1101 56-57 0.10428818378517946 1101 58-59 0.10464086119617377 1101 60-61 0.10838850491654028 1101 62-63 0.0724142486087942 1101 64-65 0.039029911414608875 1101 66-67 -0.09981716568268695 1101 68-69 -0.1051764968478679 1101 70-71 -0.09040131246579364 1101 72-73 -0.1698797367930922 1101 74-75 -0.07942257760446125 1101 76-77 -0.07003388626777252 1101 78-79 -0.16084836897650234 1101 80-81 -0.17634016473999736 1101 82-83 -0.010519556866903201 1101 84-85 -0.05437917661128466 1101 86-87 0.05700699884652494 1101 88-89 -0.019295878453043258 1101 90-91 -0.041487453509731154 1101 92-93 0.0680440873621535 1101 94-95 0.008191261298158281 1101 96-97 0.11121408873395211 1101 98-99 0.12547600286123384 1101 100-101 0.10963171828260698 1101 102-103 0.11243893679495187 1101 104-105 0.123057823711342 1101 106-107 0.14856229599893567 1101 108-109 0.14394906047125744 1101 110-111 0.12959522824062475 1101 112-113 0.19428181290312807 1101 114-115 0.08002644457692298 1101 116-117 0.1287279478044283 1101 118-119 0.18265464969871914 1101 120-121 0.25567000332940637 1101 122-123 0.21041182990072826 1101 124-125 0.23889749621390166 1102 1 0.009454129331061267 1102 2 0.08808005325819579 1102 3 -0.005469982681582053 1102 4 0.054336700468148535 1102 5 -0.10467440379593995 1102 6 -0.007050574372847507 1102 7 0.09715002937677752 1102 8 0.0703453823160416 1102 9 0.07493218929536027 1102 10-11 0.1476508665127838 1102 12-13 0.036390469847845 1102 14-15 0.09037735041334827 1102 16-17 0.20941081789266747 1102 18-19 0.06707599474926695 1102 20-21 0.126231244088153 1102 22-23 0.1979639369741193 1102 24-25 0.1838070157163969 1102 26-27 0.13036700877962915 1102 28-29 0.04463956500544697 1102 30-31 0.03480683261356887 1102 32-33 0.013297076686789921 1102 34-35 -0.008614777176354949 1102 36-37 0.07798943038514494 1102 38-39 0.028845246761108 1102 40-41 0.0712455760699271 1102 42-43 0.0761990280218825 1102 44-45 0.2280196744562275 1102 46-47 0.06722322195538055 1102 48-49 -0.11332843138558246 1102 50-51 -0.09270598746118708 1102 52-53 -0.09902961419207656 1102 54-55 -0.08213041601295856 1102 56-57 -0.0938379178883153 1102 58-59 -0.21682395196702942 1102 60-61 -0.14923235437122884 1102 62-63 -0.19934094988555273 1102 64-65 -0.23874893926342367 1102 66-67 -0.19675521564160903 1102 68-69 -0.12278439940199348 1102 70-71 -0.18060774237653732 1102 72-73 -0.14730113612032625 1102 74-75 -0.1412633291652341 1102 76-77 -0.16720587725072988 1102 78-79 -0.09041588093251107 1102 80-81 -0.09586576921200418 1102 82-83 -0.02595834771221206 1102 84-85 -0.08273871016887568 1102 86-87 -0.1557296634427061 1102 88-89 -0.16425767540101077 1102 90-91 -0.04853509145154078 1102 92-93 0.024664486668321217 1102 94-95 -0.1374275895203283 1102 96-97 -0.04525163736983018 1102 98-99 -0.0159788732948698 1102 100-101 -0.12480352262837613 1102 102-103 -0.1294850503676983 1102 104-105 -0.12128457816510974 1102 106-107 -0.02790429037577269 1102 108-109 -0.05038833778853302 1102 110-111 -0.1042768863404433 1102 112-113 0.007278663258119877 1102 114-115 0.022918499887076393 1102 116-117 -0.015654483005214104 1102 118-119 -0.03281512845671841 1102 120-121 -0.024500927237944836 1102 122-123 0.12457873650689777 1102 124-125 0.376754596433706 1103 1 0.02420364844120826 1103 2 0.0754288865775905 1103 3 0.09887307995955297 1103 4 0.01599263423079833 1103 5 -0.1946175333434823 1103 6 0.007582694841836712 1103 7 -0.02137217518958323 1103 8 0.011953645258252266 1103 9 0.0373926726942031 1103 10-11 0.027131923634129862 1103 12-13 0.028713795096656725 1103 14-15 0.04351317043509084 1103 16-17 0.08322113820712929 1103 18-19 0.05430606918265113 1103 20-21 0.04382470398612526 1103 22-23 0.03669815124190734 1103 24-25 0.07252157622488653 1103 26-27 0.130652615537187 1103 28-29 0.1023584744308863 1103 30-31 -0.08713847139246411 1103 32-33 -0.1311028564067982 1103 34-35 -0.03479609356006108 1103 36-37 2.1741399307018128E-4 1103 38-39 -0.10290017433113974 1103 40-41 0.06308424797759216 1103 42-43 -0.019886528555851157 1103 44-45 0.05285742640069202 1103 46-47 0.05175362255753413 1103 48-49 -0.04060283131867237 1103 50-51 -0.2162691722065233 1103 52-53 -0.1901528056280526 1103 54-55 -0.22898121470827704 1103 56-57 -0.25766705563189163 1103 58-59 -0.30451944414733134 1103 60-61 -0.21725861513898082 1103 62-63 -0.24124987352860927 1103 64-65 -0.3172677985090573 1103 66-67 -0.2936009963558277 1103 68-69 -0.30777018178888227 1103 70-71 -0.1936490570875833 1103 72-73 -0.14028223505818715 1103 74-75 -0.14817517996393548 1103 76-77 -0.21427746711440676 1103 78-79 -0.2553209574567177 1103 80-81 -0.21818783996553748 1103 82-83 -0.09995224250187817 1103 84-85 -0.11462379840170911 1103 86-87 -0.11647148831516319 1103 88-89 -0.3042334218256926 1103 90-91 -0.21380355344034285 1103 92-93 -0.05289970167324043 1103 94-95 -0.017157454871167488 1103 96-97 -0.05039590432642882 1103 98-99 -0.06226514911655556 1103 100-101 -1.635627722222921E-4 1103 102-103 -0.017071819624199236 1103 104-105 -0.06388180154889511 1103 106-107 -0.010006406291132919 1103 108-109 -0.08134760850861511 1103 110-111 0.0388752421198717 1103 112-113 -0.03135142790186762 1103 114-115 -0.015369114062913525 1103 116-117 0.0045537632103958 1103 118-119 -0.06230371254971345 1103 120-121 -0.11393096821812776 1103 122-123 -0.04904340951635078 1103 124-125 -0.154313815375275 1104 1 0.005978619356945103 1104 2 0.01590919237781918 1104 3 0.05067131828429439 1104 4 -0.007627109827701872 1104 5 0.0022330566515265104 1104 6 -0.04602707746994383 1104 7 0.049222805697901606 1104 8 -3.6312389580928084E-4 1104 9 -0.003963484859390576 1104 10-11 0.056032538558511646 1104 12-13 -0.027005001645903803 1104 14-15 -0.022008684309795967 1104 16-17 -0.030440154796025354 1104 18-19 0.0568222732710737 1104 20-21 0.06470471063395422 1104 22-23 0.06501874289887866 1104 24-25 0.08263292739656691 1104 26-27 0.08808738369407365 1104 28-29 0.045021761783395675 1104 30-31 0.0875083625779638 1104 32-33 -0.0034993995346042084 1104 34-35 -0.01484429033586565 1104 36-37 0.08394186139210547 1104 38-39 0.07254938525004917 1104 40-41 -0.06216338373256747 1104 42-43 0.010360272175411467 1104 44-45 0.11166829560461622 1104 46-47 -0.021942571559137036 1104 48-49 0.06663831875611947 1104 50-51 0.03980445252340559 1104 52-53 0.055087347272056775 1104 54-55 0.17607861577202755 1104 56-57 0.20868743996391714 1104 58-59 0.20064508967350037 1104 60-61 0.11020357532138547 1104 62-63 0.05300058350977821 1104 64-65 0.16189125085109168 1104 66-67 0.19751334633986772 1104 68-69 0.16948177101151174 1104 70-71 0.20766970641582105 1104 72-73 0.0852994501670068 1104 74-75 0.14939753068922101 1104 76-77 -0.010789848699914728 1104 78-79 0.020168489111341614 1104 80-81 0.056160672582251436 1104 82-83 0.06613467789033933 1104 84-85 0.0862238000692912 1104 86-87 0.03406698600758773 1104 88-89 0.10716594663034584 1104 90-91 0.04887967740559418 1104 92-93 -0.1151009647611545 1104 94-95 -0.12230453813525344 1104 96-97 -0.07602163143295826 1104 98-99 -0.01094472365537058 1104 100-101 -0.04818084713320303 1104 102-103 -0.050165554043346106 1104 104-105 0.012100415502786177 1104 106-107 0.008083173233544017 1104 108-109 -0.016559508126363198 1104 110-111 0.007294720265129229 1104 112-113 0.07977537947722624 1104 114-115 0.00739222879898449 1104 116-117 -0.11141432720010869 1104 118-119 -0.0799296058520298 1104 120-121 -0.041841056301890944 1104 122-123 0.05898384659135303 1104 124-125 0.017787730245466093 1105 1 -0.012747981681151543 1105 2 0.09090642620147094 1105 3 -0.08930381090026884 1105 4 -0.016997429003168918 1105 5 -0.130853880555442 1105 6 0.01594518149700974 1105 7 -0.012883220603072232 1105 8 0.006582043057377973 1105 9 0.05902854359837306 1105 10-11 0.09543651425579469 1105 12-13 0.05147720151859403 1105 14-15 -0.027509659490171146 1105 16-17 0.124762568772951 1105 18-19 0.053286934748882686 1105 20-21 0.11497810794440255 1105 22-23 0.14300964011437145 1105 24-25 0.06176687473848119 1105 26-27 0.13629316739724118 1105 28-29 0.15115169115093607 1105 30-31 0.16083722048567495 1105 32-33 0.14770062556741692 1105 34-35 0.2613811257878922 1105 36-37 0.2581725753628774 1105 38-39 0.23516174568199943 1105 40-41 0.13454560956090234 1105 42-43 0.15910624470521384 1105 44-45 0.07267470487651195 1105 46-47 0.038710180001892525 1105 48-49 0.06678158191940042 1105 50-51 -0.0741450858141448 1105 52-53 -0.025074379086213128 1105 54-55 -0.2127132505134881 1105 56-57 -0.007793934173413675 1105 58-59 -0.12762242517715094 1105 60-61 0.03685077140943349 1105 62-63 -0.006963513998350379 1105 64-65 0.006896609801692932 1105 66-67 -0.001814431520486437 1105 68-69 0.08250451641264789 1105 70-71 0.1083986558137866 1105 72-73 0.030825180561429022 1105 74-75 -0.059619295920249726 1105 76-77 -0.01513687419332399 1105 78-79 0.08084312236589142 1105 80-81 0.04393788886751082 1105 82-83 0.0759896972205567 1105 84-85 0.030875914171176078 1105 86-87 -0.050199828628869625 1105 88-89 -0.008011856579742016 1105 90-91 0.006818520962227126 1105 92-93 -0.00986488193110091 1105 94-95 -0.04271958018939159 1105 96-97 -0.03470156253452217 1105 98-99 -0.06241748548841031 1105 100-101 0.020570608205851215 1105 102-103 0.0673804264134148 1105 104-105 -0.02185708281686516 1105 106-107 0.012798471757641039 1105 108-109 -0.1168259693528313 1105 110-111 -0.07850752867753386 1105 112-113 -0.0723795957593012 1105 114-115 0.0088244475696726 1105 116-117 -0.032395403250966126 1105 118-119 0.14290241375396562 1105 120-121 -0.01080909912064243 1105 122-123 -0.07121635851856212 1105 124-125 -0.20670069227434595 1106 1 0.012876327825040335 1106 2 0.045353860637767696 1106 3 -0.022933129634189697 1106 4 -0.08852009623451806 1106 5 -0.19049509739488713 1106 6 0.013481808897232384 1106 7 0.006282865116901348 1106 8 -0.03582111729262749 1106 9 0.05195249390898482 1106 10-11 0.03458383519929953 1106 12-13 0.04273167361849062 1106 14-15 -0.07924722544645846 1106 16-17 -0.14461428361424566 1106 18-19 -0.1315084411533718 1106 20-21 -0.013003853759904871 1106 22-23 0.008262920976122246 1106 24-25 -0.04986807977319785 1106 26-27 -0.03753758320173972 1106 28-29 -0.015094235282575141 1106 30-31 -0.1443497412464012 1106 32-33 -0.03398975879326116 1106 34-35 -0.05971029261824157 1106 36-37 0.0049456444983064785 1106 38-39 -0.07974110337575269 1106 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-0.3324074193587663 2216 102-103 -0.28615668601335287 2216 104-105 -0.31272898833552887 2216 106-107 -0.3509295968376662 2216 108-109 -0.3477260159209976 2216 110-111 -0.409368345902152 2216 112-113 -0.4070866232825523 2216 114-115 -0.3857323475797614 2216 116-117 -0.49621567414693146 2216 118-119 -0.39713720254568585 2216 120-121 -0.4727512985524882 2216 122-123 -0.6508629341571428 2216 124-125 -0.9498442303074626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70.0 3 3.0 4 12.0 5 20.0 6 40.0 7 104.0 8 192.0 9 387.0 10 729.0 11 1095.0 12 1415.0 13 2295.0 14 4020.0 15 4402.0 16 1667.0 17 1169.0 18 1007.0 19 951.0 20 985.0 21 1005.0 22 1119.0 23 1287.0 24 1554.0 25 1982.0 26 2624.0 27 3307.0 28 4277.0 29 5666.0 30 7240.0 31 9290.0 32 12122.0 33 16100.0 34 23407.0 35 37189.0 36 78512.0 37 266245.0 38 262606.0 39 3032.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.33515019044721 20.5182324707544 15.404682194932034 23.74193514386636 2 13.07910270613481 20.436764862796345 45.04674448412453 21.43738794694432 3 19.348541153193075 25.458059059946493 29.508896403368606 25.684503383491826 4 11.360549684044962 18.17245335760683 39.99633789866518 30.47065905968303 5 11.728773594236499 38.43903841411388 35.44928121072572 14.382906780923896 6 28.63974012253549 38.06240589519277 19.888766965211353 13.409087017060386 7 27.041061640542356 32.02402233091433 22.565525926491876 18.369390102051437 8 23.273312634117875 39.65173152845308 20.660838041592513 16.414117795836532 9 24.67571302298561 18.47411566180626 22.63652853870301 34.21364277650512 10-11 23.478284924656876 26.866716636346748 30.51781849413866 19.137179944857714 12-13 26.265302116773608 24.389199699128078 29.71195860508189 19.633539579016425 14-15 20.28797552978619 29.719533095252835 26.772200172039724 23.22029120292125 16-17 19.105313804747954 30.60431956992675 30.878911485766864 19.411455139558427 18-19 20.26327610531571 28.132381011345924 32.38437046765561 19.21997241568275 20-21 20.886036344905765 28.355125917465486 30.463905065087932 20.294932672540817 22-23 19.669645538805256 30.385549659871113 30.68497373288158 19.259831068442047 24-25 20.72064358137703 28.400913154188956 32.18255970344883 18.695883560985184 26-27 18.820039334656784 29.977329221592697 31.765699283519094 19.436932160231425 28-29 18.749102587577575 30.180062097646378 29.885842553354053 21.184992761422002 30-31 19.86064255388097 28.331359574880093 31.43788852194692 20.370109349292015 32-33 19.01434147382454 29.413853018006208 32.26805264468264 19.303752863486608 34-35 19.293543768038813 29.8376951419196 31.92772750804543 18.941033581996162 36-37 18.65583756077705 29.194719724104136 31.776698760549948 20.372743954568868 38-39 20.055998535159468 29.728359022930288 30.69624713651339 19.51939530539686 40-41 21.69623791539492 27.588730212467745 31.91396169547388 18.801070176663455 42-43 19.169651454894897 30.092593202454925 31.777950198056455 18.959805144593727 44-45 18.917980785823715 30.08923408072694 30.656201136305256 20.33658399714409 46-47 20.89406647372574 28.405589578555368 30.40551844421289 20.294825503506 48-49 20.23278054923616 29.08894032223857 30.075138942495787 20.603140186029478 50-51 18.9430095359538 30.73458064329157 30.168667429823998 20.153742390930635 52-53 19.099966145322192 29.844413385375567 30.707246613544243 20.348373855757995 54-55 19.41802886737002 28.87059740991955 30.93909187790712 20.772281844803306 56-57 20.125617979600253 29.226598447954032 28.94588125570557 21.701902316740153 58-59 20.35061327024332 29.988460428887393 28.437007246481816 21.223919054387473 60-61 19.68168699045087 28.13659637978889 31.62573587818639 20.555980751573845 62-63 20.17027453904287 28.012769931776898 31.172320310040348 20.644635219139882 64-65 20.57014175493692 29.37281904081926 29.42432557398169 20.63271363026213 66-67 20.447566744431434 30.76086083092816 28.50708774684605 20.284484677794364 68-69 21.152587116516735 28.346969611145433 30.839833124101762 19.660610148236067 70-71 22.1630899704529 28.15760735687178 29.92358327394494 19.755719398730385 72-73 20.859619009730913 29.59880230844114 29.46200042944066 20.079578252387282 74-75 20.005611709239695 30.281494400805137 30.662392458705856 19.050501431249316 76-77 20.375246829581876 28.62103442506985 31.15170452374899 19.852014221599283 78-79 19.946267225378627 28.555169293148573 30.579073066825448 20.919490414647353 80-81 20.553807202220444 29.204797089288093 30.272207417204232 19.969188291287228 82-83 20.06080668978972 29.132081983647005 31.36787388671461 19.439237439848668 84-85 19.846481550517897 29.004501223115497 31.291602063949775 19.857415162416828 86-87 18.74943191323718 30.835617755658802 29.290092435126137 21.12485789597788 88-89 19.85287046831426 29.038290035791114 30.282877568575483 20.825961927319145 90-91 20.123340020892584 29.005652590298514 31.087797597484457 19.783209791324442 92-93 19.267988096853358 30.373573855231072 30.858670551831246 19.49976749608432 94-95 19.2067941648378 30.312057877389933 30.552006133401484 19.929141824370785 96-97 20.53898754753816 29.477544600574078 30.036607840321842 19.946860011565917 98-99 20.63331475912145 28.79806406654047 31.126295419546658 19.442325754791423 100-101 19.8491292294499 28.953277220594988 31.18327446084414 20.01431908911097 102-103 19.34215006892809 29.98954716914024 30.41082194027462 20.257480821657055 104-105 19.9214213966968 29.084424245418166 30.44838545011444 20.545768907770587 106-107 19.94444616435478 28.35485160723425 30.374260539189695 21.32644168922128 108-109 19.162061584060595 29.54566112300346 30.083912399143752 21.208364893792194 110-111 19.125967769345177 29.78714014525268 30.476451439945933 20.61044064545621 112-113 19.776104655758438 28.469488577978026 30.065739203752006 21.688667562511526 114-115 19.39114735071965 29.087116239517783 28.912168424158867 22.609567985603693 116-117 19.955672065497325 29.130829979647356 27.91849719739433 22.99500075746099 118-119 19.94228885737602 28.140999083814204 29.82398826017569 22.092723798634086 120-121 19.370746316630473 28.048079260039398 30.610383186354227 21.9707912369759 122-123 20.214354450206258 28.66668027898221 29.517801286100354 21.601163984711174 124-125 20.611919870270672 28.986693785822776 28.71447032678671 21.686916017119845 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 122.0 1 75.5 2 80.5 3 113.5 4 59.0 5 36.0 6 103.5 7 338.0 8 522.0 9 499.5 10 355.5 11 216.0 12 206.5 13 249.0 14 296.5 15 349.5 16 414.0 17 509.5 18 615.0 19 739.0 20 955.5 21 1218.0 22 1531.0 23 1952.5 24 2561.0 25 3302.5 26 4297.0 27 5464.0 28 6719.0 29 8494.0 30 11077.0 31 14508.5 32 19043.5 33 25251.5 34 32989.0 35 40817.0 36 46735.5 37 48542.0 38 44815.0 39 38206.5 40 33087.5 41 30094.0 42 27510.5 43 25783.5 44 25169.0 45 24687.0 46 24216.5 47 24917.5 48 26674.0 49 25114.0 50 21473.5 51 18795.5 52 16217.0 53 14048.5 54 12220.5 55 10343.0 56 8289.5 57 6518.0 58 5179.0 59 4051.5 60 3019.5 61 2140.5 62 1561.0 63 1101.5 64 706.0 65 510.0 66 380.5 67 269.0 68 192.5 69 124.5 70 92.0 71 84.5 72 69.0 73 59.0 74 42.5 75 23.5 76 14.5 77 8.5 78 6.5 79 3.5 80 1.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 2.5 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016334552716475638 2 0.0 3 0.0 4 0.0 5 0.1103899611000531 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.586513192127273E-5 18-19 0.0 20-21 0.005071615157937999 22-23 3.951907915276363E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.903815830552726E-4 92-93 0.0 94-95 6.586513192127272E-4 96-97 0.0 98-99 0.0013173026384254544 100-101 6.586513192127273E-5 102-103 3.293256596063636E-4 104-105 0.0019100888257169092 106-107 0.005730266477150727 108-109 2.634605276850909E-4 110-111 0.004742289498331636 112-113 0.008825927677450544 114-115 0.005730266477150727 116-117 0.0015807631661105452 118-119 1.9759539576381816E-4 120-121 7.24516451134E-4 122-123 0.0013831677703467272 124-125 0.0010538421107403636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 759127.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.10155066010546 #Duplication Level Percentage of deduplicated Percentage of total 1 88.86237835526255 69.40289544890996 2 8.30611747072792 12.974413088576872 3 1.5165364172447318 3.5533153745800274 4 0.5110611541554504 1.5965867448673547 5 0.21846735721184254 0.8531319683430038 6 0.12461953352880983 0.583978728668343 7 0.08093748777320357 0.44249403111343677 8 0.056886519267460856 0.3554340293155727 9 0.043017939638031716 0.3023791012739783 >10 0.23397191408205484 3.40355617215184 >50 0.024941714290471706 1.3458020878502353 >100 0.018367927315209982 3.01916413250633 >500 0.0016851309389543986 0.9264122180201149 >1k 0.001011078563372639 1.2404368738229268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2031 0.2675441658642098 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1936 0.255029790799168 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1595 0.21010977082885998 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.2074751655520091 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1240 0.16334552716475637 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1048 0.13805331650698763 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 934 0.12303606642893745 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 922 0.1214553032628269 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 911 0.1200062703605589 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 841 0.11078515189158072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3173026384254546E-4 2 0.0 0.0 0.0 0.0 1.3173026384254546E-4 3 0.0 0.0 0.0 0.0 1.3173026384254546E-4 4 0.0 0.0 0.0 0.0 1.3173026384254546E-4 5 0.0 0.0 0.0 0.0 1.3173026384254546E-4 6 0.0 0.0 0.0 0.0 1.3173026384254546E-4 7 0.0 0.0 0.0 0.0 1.3173026384254546E-4 8 0.0 0.0 0.0 0.0 1.3173026384254546E-4 9 0.0 0.0 0.0 2.634605276850909E-4 1.3173026384254546E-4 10-11 0.0 0.0 0.0 4.6105592344890907E-4 1.9759539576381818E-4 12-13 0.0 0.0 0.0 5.269210553701818E-4 2.634605276850909E-4 14-15 0.0 0.0 0.0 5.927861872914546E-4 2.634605276850909E-4 16-17 0.0 0.0 0.0 6.586513192127272E-4 2.634605276850909E-4 18-19 0.0 0.0 0.0 6.586513192127272E-4 2.634605276850909E-4 20-21 0.0 0.0 0.0 7.24516451134E-4 2.634605276850909E-4 22-23 0.0 0.0 0.0 7.903815830552727E-4 2.634605276850909E-4 24-25 0.0 0.0 0.0 9.221118468978181E-4 2.634605276850909E-4 26-27 0.0 0.0 0.0 9.221118468978181E-4 2.634605276850909E-4 28-29 0.0 0.0 0.0 0.0013831677703467272 2.634605276850909E-4 30-31 0.0 0.0 0.0 0.002898065804536 2.634605276850909E-4 32-33 0.0 0.0 0.0 0.004939884894095454 2.634605276850909E-4 34-35 0.0 0.0 0.0 0.008035546094395272 2.634605276850909E-4 36-37 0.0 0.0 0.0 0.013699947439624726 2.634605276850909E-4 38-39 0.0 0.0 0.0 0.024699424470477273 2.634605276850909E-4 40-41 0.0 0.0 0.0 0.039650809416606175 2.634605276850909E-4 42-43 0.0 0.0 0.0 0.053680082515837266 2.634605276850909E-4 44-45 0.0 0.0 0.0 0.07040982602384055 2.634605276850909E-4 46-47 0.0 0.0 0.0 0.09089388205135637 2.634605276850909E-4 48-49 0.0 0.0 0.0 0.11328802690458908 2.634605276850909E-4 50-51 0.0 0.0 0.0 0.13950234940925565 2.634605276850909E-4 52-53 0.0 0.0 0.0 0.16749503047579656 2.634605276850909E-4 54-55 0.0 0.0 0.0 0.19924202406185 2.634605276850909E-4 56-57 0.0 0.0 0.0 0.23797072163155836 2.634605276850909E-4 58-59 0.0 0.0 0.0 0.2815734389634409 2.634605276850909E-4 60-61 0.0 0.0 0.0 0.33044536684902526 2.634605276850909E-4 62-63 0.0 0.0 0.0 0.38353266317757106 2.634605276850909E-4 64-65 0.0 0.0 0.0 0.43286564698660435 2.634605276850909E-4 66-67 0.0 0.0 0.0 0.4925394565072775 2.634605276850909E-4 68-69 0.0 0.0 0.0 0.566571864786788 2.634605276850909E-4 70-71 0.0 0.0 0.0 0.6511426941737022 2.634605276850909E-4 72-73 0.0 0.0 0.0 0.7345279511860334 2.634605276850909E-4 74-75 0.0 0.0 0.0 0.8122488068531353 2.634605276850909E-4 76-77 0.0 0.0 0.0 0.9078849784028232 2.634605276850909E-4 78-79 0.0 0.0 0.0 1.0199215678009081 2.634605276850909E-4 80-81 0.0 0.0 0.0 1.1371615026207738 2.634605276850909E-4 82-83 0.0 0.0 0.0 1.258287480223994 2.634605276850909E-4 84-85 0.0 0.0 0.0 1.3822456584998295 2.634605276850909E-4 86-87 0.0 0.0 0.0 1.5069283532267987 2.634605276850909E-4 88-89 0.0 0.0 0.0 1.6516340480578349 2.634605276850909E-4 90-91 0.0 0.0 0.0 1.8160992824652529 2.634605276850909E-4 92-93 0.0 0.0 0.0 1.983726043204892 2.634605276850909E-4 94-95 0.0 0.0 0.0 2.1446345604885613 2.634605276850909E-4 96-97 0.0 0.0 0.0 2.3179257225734298 2.634605276850909E-4 98-99 0.0 0.0 0.0 2.518023993350256 2.634605276850909E-4 100-101 0.0 0.0 0.0 2.7326125931497627 2.634605276850909E-4 102-103 0.0 0.0 0.0 2.961559791708107 2.634605276850909E-4 104-105 0.0 0.0 0.0 3.189321417891868 2.634605276850909E-4 106-107 0.0 0.0 0.0 3.414184978271093 2.634605276850909E-4 108-109 0.0 0.0 0.0 3.656634528873298 2.634605276850909E-4 110-111 0.0 0.0 0.0 3.931292128985005 2.634605276850909E-4 112-113 0.0 0.0 0.0 4.2359183641208915 2.634605276850909E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCGA 35 0.007750961 51.073536 1 TGTACGA 55 8.480065E-4 43.32083 2 TAGGACC 1195 0.0 38.381535 4 TGTACCG 65 0.0019279394 36.65609 5 GTGTAGC 505 0.0 34.217583 1 TACCTGG 850 0.0 30.133463 2 CACAGCG 80 0.0053630434 29.74776 7 GTACATG 2775 0.0 29.202404 1 GTACCTG 910 0.0 28.81071 1 ACATGGG 2455 0.0 28.145304 3 TACATGG 2720 0.0 28.031126 2 ACCTGGG 910 0.0 27.492067 3 CATGGGG 1115 0.0 26.711277 4 GTAGCCC 495 0.0 26.473843 3 GAGTACT 2150 0.0 26.011995 12-13 TAGAACG 140 1.0475178E-4 25.528347 4 GTACTTT 2355 0.0 23.874 14-15 GTAGGAC 4565 0.0 23.617682 3 GTATCAA 4800 0.0 23.08949 1 GTATAGA 465 0.0 23.065468 1 >>END_MODULE