FastQCFastQC Report
Fri 27 May 2016
SRR939951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921034
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC5500.02335.73108-109
CCGTTCG700.01764.6311108-109
CCGCGCC4600.01503.7726108-109
CTCCGCC3300.01278.4319110-111
CGTCGTC905.3592885E-81004.48236110-111
CGTGTCG807.4200216E-8926.4313108-109
CCGCGCT552.9945411E-5898.3576108-109
CAGCGCG1356.530172E-10731.9951108-109
CCTCGCG2400.0720.5577108-109
CGCCGCC3400.0709.0463110-111
CATCGCG1052.2001404E-7705.8525108-109
CGTCGCC2450.0705.8524108-109
CCGCACC2450.0705.8524108-109
TTGTTCG3350.0663.712108-109
CCGTTCC3350.0663.712108-109
CCGCTCG1551.3005774E-9637.5441108-109
CGTCGAT1009.899501E-5602.6894110-111
CGACGCC1254.4162516E-7592.9161108-109
CCGCGCG2550.0581.2903108-109
CGCCGCG1551.0430904E-6478.15808108-109
Produced by FastQC (version 0.11.5)