##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939951_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921034 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.883345635735754 34.0 31.0 34.0 30.0 34.0 2 31.97017387511101 34.0 31.0 34.0 30.0 34.0 3 32.125385079077205 34.0 31.0 34.0 30.0 34.0 4 35.43030940628849 37.0 35.0 37.0 33.0 37.0 5 35.44437266597051 37.0 35.0 37.0 33.0 37.0 6 35.4984664508801 37.0 37.0 37.0 35.0 37.0 7 35.496578405171384 37.0 37.0 37.0 35.0 37.0 8 35.47495255159461 37.0 37.0 37.0 35.0 37.0 9 37.20705151496538 39.0 39.0 39.0 35.0 39.0 10-11 37.178828953573955 39.0 39.0 39.0 35.0 39.0 12-13 37.129712436115135 39.0 38.0 39.0 35.0 39.0 14-15 38.639377803828566 41.0 39.5 41.0 35.0 41.0 16-17 38.61779645753276 41.0 39.0 41.0 35.0 41.0 18-19 38.605866944572554 41.0 39.0 41.0 35.0 41.0 20-21 38.636918971762086 41.0 39.0 41.0 35.0 41.0 22-23 38.59435569594291 41.0 39.0 41.0 34.0 41.0 24-25 38.58691959642567 41.0 39.0 41.0 34.0 41.0 26-27 38.53324355529366 41.0 39.0 41.0 34.0 41.0 28-29 38.47514906034979 41.0 39.0 41.0 34.0 41.0 30-31 38.40261442535635 41.0 39.0 41.0 34.0 41.0 32-33 38.30644147891188 40.5 39.0 41.0 34.0 41.0 34-35 38.23683729699735 40.0 38.5 41.0 34.0 41.0 36-37 38.41272096173207 41.0 39.0 41.0 34.0 41.0 38-39 38.393224169900165 41.0 39.0 41.0 34.0 41.0 40-41 38.34040391789006 41.0 39.0 41.0 34.0 41.0 42-43 38.2641837677001 41.0 39.0 41.0 33.5 41.0 44-45 38.16271913979659 41.0 39.0 41.0 33.0 41.0 46-47 38.078934834052916 41.0 38.0 41.0 33.0 41.0 48-49 37.991709933296335 40.5 38.0 41.0 33.0 41.0 50-51 37.88194795094725 40.0 38.0 41.0 33.0 41.0 52-53 37.77152824989042 40.0 38.0 41.0 33.0 41.0 54-55 37.62980717676002 40.0 38.0 41.0 33.0 41.0 56-57 37.46402432231808 40.0 37.5 41.0 32.0 41.0 58-59 37.309918772910834 40.0 37.0 41.0 32.0 41.0 60-61 37.09425418810911 40.0 37.0 41.0 31.5 41.0 62-63 36.85251380246263 40.0 36.0 41.0 31.0 41.0 64-65 36.57636642558123 39.0 36.0 41.0 31.0 41.0 66-67 36.28948732817847 39.0 35.0 41.0 31.0 41.0 68-69 35.93369221991907 38.5 35.0 40.5 30.0 41.0 70-71 35.55567938932887 37.5 35.0 40.0 30.0 41.0 72-73 35.158370960638905 37.0 35.0 39.5 30.0 41.0 74-75 34.740975953575 36.5 35.0 39.0 29.0 41.0 76-77 34.29457885701138 36.0 34.5 39.0 29.0 40.5 78-79 33.85249298034288 36.0 34.0 37.5 29.0 39.5 80-81 33.42594977496494 35.0 34.0 37.0 29.0 39.0 82-83 33.21649929152737 35.0 34.0 37.0 29.0 39.0 84-85 32.815898625427764 35.0 34.0 36.0 29.0 37.5 86-87 32.18254049642016 35.0 34.0 36.0 27.5 37.0 88-89 16.603971871398425 18.0 17.0 18.5 2.0 19.0 90-91 2.7916333078956437 2.0 2.0 2.0 2.0 2.0 92-93 2.3006190936755937 2.0 2.0 2.0 2.0 2.0 94-95 2.1033133718611956 2.0 2.0 2.0 2.0 2.0 96-97 2.0000161371428096 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28220272318694484 1101 2 0.3055135833105638 1101 3 0.3298627015794011 1101 4 0.319056809749263 1101 5 0.3358029385157977 1101 6 0.33376894155405523 1101 7 0.29561095099934676 1101 8 0.40870195169141255 1101 9 0.4012942322434796 1101 10-11 0.3840629807777205 1101 12-13 0.43373117176299303 1101 14-15 0.5258615283969306 1101 16-17 0.5007870405903816 1101 18-19 0.5024501861699662 1101 20-21 0.47045959012424987 1101 22-23 0.4066376365380151 1101 24-25 0.4584980905166063 1101 26-27 0.4570815825344994 1101 28-29 0.46886876312321135 1101 30-31 0.426117814755159 1101 32-33 0.46398032829905134 1101 34-35 0.4972723090586939 1101 36-37 0.4946317732824923 1101 38-39 0.48419884993379014 1101 40-41 0.4771835511601026 1101 42-43 0.549451844938659 1101 44-45 0.5693761077569022 1101 46-47 0.5755221793593535 1101 48-49 0.5739747899975924 1101 50-51 0.5327913437779728 1101 52-53 0.5254253256044521 1101 54-55 0.5088053907702346 1101 56-57 0.5472613698830031 1101 58-59 0.4624975982965154 1101 60-61 0.5007226291063489 1101 62-63 0.4590148425860363 1101 64-65 0.500277769714323 1101 66-67 0.49481421594984454 1101 68-69 0.5849986200866795 1101 70-71 0.5666800102901206 1101 72-73 0.5829173147284976 1101 74-75 0.5256278897755706 1101 76-77 0.5441523508381678 1101 78-79 0.5763852736023765 1101 80-81 0.5181911451295491 1101 82-83 0.6008423540475221 1101 84-85 0.6219611840756727 1101 86-87 0.6370441667878737 1101 88-89 0.7826580621390988 1101 90-91 -0.05508699587541477 1101 92-93 -0.1410821662380397 1101 94-95 0.019095786815793936 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.14041376551723417 1102 2 0.1414821111935538 1102 3 0.2723760480216981 1102 4 0.24650561767239054 1102 5 0.2640504915228874 1102 6 0.22498953189801796 1102 7 0.27577665576716726 1102 8 0.32647356995982335 1102 9 0.4210516071535437 1102 10-11 0.3851363119028477 1102 12-13 0.332560463969223 1102 14-15 0.3642400870243634 1102 16-17 0.3557734213735628 1102 18-19 0.35461392556781846 1102 20-21 0.32722928217959435 1102 22-23 0.35837473291012145 1102 24-25 0.3739067081288212 1102 26-27 0.27890638618910657 1102 28-29 0.2749738817107925 1102 30-31 0.31665942997323526 1102 32-33 0.2852803458430131 1102 34-35 0.29271595050018107 1102 36-37 0.3510107323972491 1102 38-39 0.354862553867477 1102 40-41 0.2879853382198476 1102 42-43 0.25596479451668586 1102 44-45 0.31209386438127495 1102 46-47 0.3597245149223127 1102 48-49 0.33658056066606434 1102 50-51 0.31775138631826394 1102 52-53 0.22255852470810567 1102 54-55 0.28620348283968156 1102 56-57 0.33235044905059397 1102 58-59 0.3166024610404179 1102 60-61 0.2479159082768092 1102 62-63 0.21899633433027077 1102 64-65 0.24703656054358447 1102 66-67 0.29998367964100225 1102 68-69 0.3084127202227265 1102 70-71 0.36139492922348637 1102 72-73 0.3197661343847642 1102 74-75 0.31209254676875986 1102 76-77 0.20228924587969033 1102 78-79 0.2938703776057636 1102 80-81 0.30117808386377476 1102 82-83 0.2822275106828016 1102 84-85 0.30884231166070464 1102 86-87 0.4783850635812854 1102 88-89 1.362863383659775 1102 90-91 0.0783210607201057 1102 92-93 -0.10216717197292358 1102 94-95 0.029931379799712943 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.03793274696676363 1103 2 0.17451788468000728 1103 3 0.1483432528400357 1103 4 0.15795470300400893 1103 5 0.1319929172783887 1103 6 0.03524851315810906 1103 7 0.11449096448517082 1103 8 0.1331081126120992 1103 9 0.1482930149269066 1103 10-11 0.05018696744112816 1103 12-13 0.0566729602649545 1103 14-15 0.061456569593637767 1103 16-17 0.02948538235926179 1103 18-19 0.0315172463573532 1103 20-21 0.05134177847531873 1103 22-23 0.05999866002351695 1103 24-25 0.006544787940896413 1103 26-27 0.08075832073406275 1103 28-29 0.06732832569065295 1103 30-31 0.022660829140498606 1103 32-33 0.14086070932880546 1103 34-35 0.11768053154160896 1103 36-37 0.08006943382388698 1103 38-39 0.11280654928837919 1103 40-41 0.12204484477658895 1103 42-43 0.1019205380712549 1103 44-45 0.10080546677961877 1103 46-47 0.1351802253244614 1103 48-49 0.020302183212962177 1103 50-51 0.03406724629869018 1103 52-53 -0.02006770142384795 1103 54-55 0.0585072103890667 1103 56-57 0.09378579980992185 1103 58-59 0.08796127707969248 1103 60-61 0.1229003131097528 1103 62-63 -0.030808404706085923 1103 64-65 -0.02034227414223011 1103 66-67 -0.015462436857930584 1103 68-69 0.016636346122744783 1103 70-71 0.08221945010341614 1103 72-73 0.13568308284695973 1103 74-75 0.0753225934121815 1103 76-77 0.15806554690903596 1103 78-79 0.1774635176266841 1103 80-81 0.20975711719344758 1103 82-83 0.22340423200358828 1103 84-85 0.2134452878004396 1103 86-87 0.17696289259871634 1103 88-89 0.6190666742738529 1103 90-91 -0.5681873809675011 1103 92-93 -0.33138771131536915 1103 94-95 -0.11783367376511888 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.05599348184318487 1104 2 -0.03338738834154498 1104 3 -0.01627042805272083 1104 4 6.244363714102974E-4 1104 5 -0.06482112139611473 1104 6 -0.0454994210781976 1104 7 0.028529638988963768 1104 8 0.05732652985376774 1104 9 0.04968154887357912 1104 10-11 0.12795764637460394 1104 12-13 0.18810664286142753 1104 14-15 0.08399256073427352 1104 16-17 0.15251545306374936 1104 18-19 0.16542132677306398 1104 20-21 0.0779454074045276 1104 22-23 -0.0447888005778978 1104 24-25 0.03630030374291948 1104 26-27 0.10989197777469428 1104 28-29 0.16088399482771365 1104 30-31 0.058345299708690845 1104 32-33 0.07022436892502526 1104 34-35 0.12307281367807121 1104 36-37 0.13544041942742524 1104 38-39 0.08003118511766871 1104 40-41 0.22234725554411483 1104 42-43 0.07618580768535566 1104 44-45 -0.024507592254373378 1104 46-47 0.03425169648895121 1104 48-49 0.05075622529490431 1104 50-51 0.046742942695317424 1104 52-53 0.1476339767429451 1104 54-55 0.1902518223662355 1104 56-57 0.13293734593820972 1104 58-59 0.10168050358961267 1104 60-61 -0.022827602385603996 1104 62-63 -0.00999317342574102 1104 64-65 -0.06992060279032586 1104 66-67 -0.01636966916051108 1104 68-69 0.0924392254139974 1104 70-71 -4.409127895073084E-4 1104 72-73 0.030793398887510648 1104 74-75 -0.028050777403052507 1104 76-77 -0.12411351433978979 1104 78-79 -0.08033953203683808 1104 80-81 0.007704131419529858 1104 82-83 -0.008706680339884088 1104 84-85 0.0621334213722875 1104 86-87 0.062251247654515396 1104 88-89 0.45064091398762685 1104 90-91 -0.6593578384170282 1104 92-93 -0.33138771131536915 1104 94-95 -0.11783367376511888 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.051949704267325814 1105 2 -0.07970230554862923 1105 3 -0.008506022488653286 1105 4 0.07307165222866274 1105 5 0.008777519511781406 1105 6 -9.881470489858657E-4 1105 7 0.04357838533605474 1105 8 -0.08783173599406524 1105 9 -0.049994231090011 1105 10-11 -0.07971331531026493 1105 12-13 -0.02069339038545337 1105 14-15 -0.0819020605126255 1105 16-17 0.011831600027548461 1105 18-19 -0.034650834066269454 1105 20-21 0.03504547358345178 1105 22-23 -0.13228891864209658 1105 24-25 -0.005328062897113739 1105 26-27 -0.023903789419073007 1105 28-29 -0.07600322835895668 1105 30-31 -0.0019679387828830386 1105 32-33 0.003473703515190607 1105 34-35 0.069081330672077 1105 36-37 -0.10124499268589204 1105 38-39 0.024422757789494653 1105 40-41 -0.04006588855907012 1105 42-43 -0.03849627848072856 1105 44-45 -0.08510279954047206 1105 46-47 -0.04601584013771287 1105 48-49 -0.06952959711512818 1105 50-51 -0.21230282150749247 1105 52-53 -0.11153274816872738 1105 54-55 -0.2164035353242184 1105 56-57 -0.1387592334742891 1105 58-59 -0.20208382389466095 1105 60-61 -0.19165229373429327 1105 62-63 -0.18824039545381055 1105 64-65 -0.21663246758131294 1105 66-67 -0.15480104615430434 1105 68-69 -0.11828383753027794 1105 70-71 -0.2519909540647447 1105 72-73 -0.20180100996975625 1105 74-75 -0.22080591728866494 1105 76-77 -0.15366186682471294 1105 78-79 -0.05794171424106054 1105 80-81 -0.19104936249370752 1105 82-83 -0.18128398720416783 1105 84-85 -0.21506444604192865 1105 86-87 -0.25827775552471977 1105 88-89 0.7578680172462633 1105 90-91 -0.8115939743740945 1105 92-93 -0.33138771131536915 1105 94-95 -0.11783367376511888 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.06218748514234207 1106 2 -0.0510299101306515 1106 3 0.03363850927576095 1106 4 -0.014511125094642807 1106 5 -0.03266903180637115 1106 6 0.0014632904994442697 1106 7 -0.08150367601572839 1106 8 0.012223387693993004 1106 9 0.08638341317029585 1106 10-11 -0.030735355483976434 1106 12-13 -0.01187500776145356 1106 14-15 0.016047678531059262 1106 16-17 0.01370695587151971 1106 18-19 -0.06688203632565148 1106 20-21 -0.03806849831326531 1106 22-23 -0.10870316434590421 1106 24-25 -0.06950456835881624 1106 26-27 -0.2125305878538768 1106 28-29 8.699000180456551E-4 1106 30-31 8.504188739024698E-4 1106 32-33 0.08923681166874786 1106 34-35 -0.015835951974878526 1106 36-37 0.05145992326027482 1106 38-39 0.0779550308485426 1106 40-41 0.04881763156327423 1106 42-43 -0.3430086982001299 1106 44-45 -0.016368492779598398 1106 46-47 -0.17998188423959505 1106 48-49 -0.018718994959819213 1106 50-51 -0.0010693139421817932 1106 52-53 -0.07689061888654436 1106 54-55 -0.09289685610540488 1106 56-57 -0.21754633120232114 1106 58-59 -0.1331342361229204 1106 60-61 0.024886562835924053 1106 62-63 0.019777726825800812 1106 64-65 0.04349944741854728 1106 66-67 0.00978783442850073 1106 68-69 0.09041874128900673 1106 70-71 0.10198132939241589 1106 72-73 0.006016701476823982 1106 74-75 -0.020775467080873966 1106 76-77 0.09037372320191395 1106 78-79 0.09340879163871563 1106 80-81 6.598819844327863E-6 1106 82-83 0.019713208145667238 1106 84-85 -0.004233289996356859 1106 86-87 0.09502279977545669 1106 88-89 0.621329432378058 1106 90-91 -0.3079430703407149 1106 92-93 -0.3159148323167602 1106 94-95 -0.11783367376511888 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.0641193934037787 1107 2 -0.06590718531088058 1107 3 0.006189931775615776 1107 4 -0.0038326711196887686 1107 5 0.010419567586502865 1107 6 -0.04243670077796935 1107 7 -0.017550850809570306 1107 8 -0.13291128035422872 1107 9 -0.06173442604470836 1107 10-11 0.027724878645479123 1107 12-13 -0.031921491458369644 1107 14-15 -0.00863327623708443 1107 16-17 0.05235542524998493 1107 18-19 0.004435380371290876 1107 20-21 -0.0992788620832954 1107 22-23 -0.0188913564612605 1107 24-25 -0.03223925136003203 1107 26-27 -0.05736279316238324 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2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21860.0 3 3650.0 4 2617.0 5 2154.0 6 2173.0 7 2330.0 8 2597.0 9 3045.0 10 3593.0 11 4272.0 12 5022.0 13 5582.0 14 6340.0 15 6989.0 16 7889.0 17 9536.0 18 12098.0 19 15080.0 20 17314.0 21 23463.0 22 29063.0 23 37825.0 24 56578.0 25 93394.0 26 184595.0 27 561156.0 28 772293.0 29 27548.0 30 978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22571490145411 20.004955664501516 14.814521762759014 23.954807671285362 2 13.758699850599424 20.72867918438738 42.38109119681834 23.131529768194856 3 18.169319724596495 25.675059901460077 29.349970862971603 26.805649510971826 4 11.876440645659237 18.092165908217396 39.56131460836832 30.470078837755043 5 13.383812869834042 38.130260744391755 35.21314835859046 13.272778027183746 6 29.8800541791556 37.769503298744326 19.690073158517755 12.660369363582321 7 27.82996032345081 31.789234339423455 22.80474994195834 17.576055395167394 8 24.38301456403166 37.21912261833992 21.407689817046442 16.99017300058198 9 25.494551371813305 17.537950916017103 21.48483577073597 35.482661941433626 10-11 23.693469573243007 27.346757430806402 29.425675798780127 19.534097197170468 12-13 25.012903432352047 24.50767191367207 29.172740975340716 21.306683678635167 14-15 21.3145163788138 27.339788712690332 26.38178876110177 24.9639061473941 16-17 21.35678882431989 29.522987295607532 28.022150634412764 21.098073245659812 18-19 21.635964534397488 28.614581627864176 28.90652757933962 20.842926258398716 20-21 22.53540336199196 28.076255957822056 28.09546466326983 21.292876016916154 22-23 21.922255046134225 28.620788381202743 27.970848895771788 21.48610767689124 24-25 22.029932838252734 28.14135512437573 28.477111805413124 21.351600231958415 26-27 21.334005540766064 28.757767952050823 28.252128801468373 21.656097705714735 28-29 21.456430534115107 28.748083373481307 27.81891481003436 21.97657128236922 30-31 21.851200967812126 28.204654368428667 28.12977282026242 21.814371843496783 32-33 21.42552802178224 28.578024788464635 28.27055704160484 21.725890148148284 34-35 21.78660266632796 28.74049696738445 28.15682616344396 21.31607420284363 36-37 21.536350094883822 28.36324822029392 28.113851216025125 21.986550468797134 38-39 21.829545963267698 28.58221145487274 27.90171855365392 21.686524028205643 40-41 22.392393887874967 27.97459597279382 28.320685630759268 21.312324508571944 42-43 21.593839567649507 28.58340872675861 28.26451796272216 21.558233742869724 44-45 21.447731506954852 28.607733443412997 28.051001726675178 21.89353332295697 46-47 21.983629649449203 28.215768174847504 27.81567114376945 21.984931031933844 48-49 21.760740185098328 28.1954812853293 27.957744609280528 22.086033920291843 50-51 21.45701779797177 28.70439271360963 28.047246443125534 21.79134304529307 52-53 21.638398903923616 28.484217353779268 28.109132894056017 21.768250848241102 54-55 21.577652542469934 28.131588344856812 28.369328362326822 21.92143075034643 56-57 21.776944083238504 28.32063357545988 27.750523936588316 22.151898404713293 58-59 22.0032284696679 28.277349594020716 27.6106253194894 22.108796616821984 60-61 21.643396212664637 27.966761650236275 28.4539992524859 21.935842884613184 62-63 21.825173318119305 27.91668445222729 28.43221410969301 21.8259281199604 64-65 21.8073500540204 28.264525319313798 27.90752998320956 22.020594643456242 66-67 21.814840341191257 28.643376431650868 27.57621676659549 21.965566460562385 68-69 22.11056322723126 27.92758042245621 28.266904039635037 21.69495231067749 70-71 22.26493648732922 27.787379088553354 28.041539087803756 21.906145336313674 72-73 22.047605612394158 28.06691604625426 27.91613787158374 21.969340469767847 74-75 21.63033033251884 28.507121685508952 28.16204710588152 21.70050087609069 76-77 21.87358474654795 27.92712153975411 28.282893483405292 21.916400230292645 78-79 21.771003055652436 27.992821559267682 28.121502527290048 22.114672857789834 80-81 21.906301502211832 28.21951615640327 28.054344691452627 21.819837649932275 82-83 21.750161697741696 28.22913318297938 28.34610214091714 21.674602978361783 84-85 21.77572645141274 28.069916531908472 28.331130092213385 21.8232269244654 86-87 21.447177663891225 28.498502093945127 27.959203858376952 22.095116383786696 88-89 30.867979567544847 17.743634846595384 34.09669178381627 17.291693802043504 90-91 30.725502838607895 14.427959952288505 16.375543692810297 38.470993516293305 92-93 22.74947964089542 17.11946501316845 34.63765355181134 25.493401794124793 94-95 15.213346172236847 6.6687006457490625 26.806555385690533 51.311397796323554 96-97 26.308120883151474 6.965007987406069 14.420324487740013 52.30654664170245 98-99 57.29379304156665 14.07276968976424 18.790237555799816 9.84319971286929 100-101 30.755389385274718 6.931653193764664 12.181909767023399 50.13104765393722 102-103 23.19792047042475 1.9771957650229253 36.48455614097203 38.340327623580286 104-105 42.44711127238796 4.594282159612244 39.007801767709495 13.950804800290298 106-107 5.124653739612189 1.5384462558466918 6.05714431678852 87.2797556877526 108-109 18.20956386583116 10.24771358594806 20.368279300201454 51.174443248019315 110-111 40.087116738480574 2.5895736800455325 24.038417165603843 33.28489241587005 112-113 27.478573391361582 2.7503500050504996 37.78457935855397 31.986497245033945 114-115 27.987428833139873 3.7883274504694446 22.432268100664093 45.79197561572659 116-117 18.994917791923942 4.022799619902738 19.335622308259502 57.64666027991382 118-119 19.654850867322065 4.149093747161298 14.651319289828502 61.54473609568814 120-121 24.989400908797315 4.129459094153156 28.89212583879015 41.98901415825939 122-123 33.84316832964083 1.5725213060141987 33.129464021001446 31.454846343343522 124-125 38.078191464686554 1.740066831826377 18.75940490381523 41.42233679967183 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 14.5 2 27.0 3 42.5 4 61.5 5 73.5 6 69.0 7 61.0 8 68.5 9 73.0 10 66.5 11 65.5 12 80.0 13 98.5 14 106.0 15 119.5 16 157.5 17 231.0 18 348.0 19 560.0 20 878.5 21 1368.5 22 2154.5 23 3330.5 24 4998.5 25 7281.5 26 10233.0 27 13917.5 28 18441.5 29 23904.5 30 30047.5 31 36993.0 32 44634.5 33 52759.5 34 60763.0 35 67147.0 36 71620.0 37 74992.5 38 77490.0 39 79335.0 40 80674.0 41 81255.0 42 81587.0 43 82007.5 44 81673.0 45 80622.0 46 78742.0 47 75732.0 48 72348.0 49 68869.0 50 64694.0 51 59548.0 52 53881.0 53 47829.5 54 41835.5 55 36157.5 56 30825.0 57 25787.0 58 21247.5 59 17222.5 60 13680.0 61 10595.5 62 8016.5 63 6070.0 64 4475.5 65 3145.5 66 2228.5 67 1665.5 68 1221.0 69 838.0 70 573.0 71 401.5 72 281.0 73 199.0 74 144.5 75 91.5 76 58.5 77 45.5 78 23.5 79 13.5 80 10.0 81 8.5 82 7.5 83 5.0 84 6.5 85 7.0 86 4.5 87 5.5 88 3.5 89 3.0 90 4.5 91 4.5 92 4.0 93 4.5 94 4.0 95 2.0 96 5.5 97 10.0 98 8.5 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0012493271852554407 3 0.04148807361035776 4 0.0462251058544513 5 0.047682654237249315 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.72608293242077E-4 12-13 0.0017438525294190526 14-15 2.6027649692821677E-5 16-17 2.863041466210385E-4 18-19 0.006793216569826458 20-21 0.0015616589815693007 22-23 4.6849769447079024E-4 24-25 0.0 26-27 0.0 28-29 8.068571404774721E-4 30-31 0.0 32-33 0.0010150783380200455 34-35 1.301382484641084E-4 36-37 2.6027649692821677E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.2055299385643354E-5 46-47 0.0 48-49 5.2055299385643354E-5 50-51 2.6027649692821677E-5 52-53 0.0 54-55 0.0014575483827980141 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.6027649692821677E-5 66-67 0.0 68-69 3.383594460066818E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.0822119754257342E-4 80-81 0.0 82-83 5.986359429348986E-4 84-85 2.6027649692821677E-5 86-87 9.890506883272238E-4 88-89 19.269206062984832 90-91 26.289123461635768 92-93 40.42781127247097 94-95 44.80477701071402 96-97 48.481755138118324 98-99 93.03828042606222 100-101 51.68760677843286 102-103 53.09958074661875 104-105 74.89568118003118 106-107 81.65904403565996 108-109 12.571016442186863 110-111 39.087283202691886 112-113 52.07263380033878 114-115 86.81553788220302 116-117 66.50139976700048 118-119 71.92038246069565 120-121 80.41619773517803 122-123 75.85792338917479 124-125 50.06473076478605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1921034.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.541986430139 #Duplication Level Percentage of deduplicated Percentage of total 1 76.94529363175278 50.43147391075401 2 14.069535888739857 18.44290660596283 3 4.556288701145956 8.958846366669118 4 1.8375981859031836 4.81759341458058 5 0.8787728478321065 2.8798259033893263 6 0.5020072896534622 1.9741532979778849 7 0.29866874248301856 1.370273986685008 8 0.20515399440257462 1.075696025377789 9 0.12577115102122252 0.7418961966182337 >10 0.539850852004449 6.016152644566149 >50 0.026652112977075603 1.1984738141023896 >100 0.013770201070798868 1.7445253727881753 >500 4.7730076007103736E-4 0.2179920436462715 >1k 1.5910025335700583E-4 0.13019041688225858 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.2055299385643354E-5 0.0 5 0.0 0.0 0.0 5.2055299385643354E-5 0.0 6 0.0 0.0 0.0 5.2055299385643354E-5 0.0 7 0.0 0.0 0.0 5.2055299385643354E-5 0.0 8 0.0 0.0 0.0 5.2055299385643354E-5 0.0 9 0.0 0.0 0.0 1.0411059877128671E-4 0.0 10-11 0.0 0.0 0.0 1.0411059877128671E-4 0.0 12-13 0.0 0.0 0.0 1.0411059877128671E-4 0.0 14-15 0.0 0.0 0.0 1.3013824846410838E-4 0.0 16-17 0.0 0.0 0.0 1.5616589815693006E-4 0.0 18-19 0.0 0.0 0.0 1.5616589815693006E-4 0.0 20-21 0.0 0.0 0.0 2.0822119754257342E-4 0.0 22-23 0.0 0.0 0.0 2.6027649692821677E-4 0.0 24-25 0.0 0.0 0.0 3.123317963138601E-4 0.0 26-27 0.0 0.0 0.0 4.1644239508514683E-4 0.0 28-29 0.0 0.0 0.0 6.506912423205419E-4 0.0 30-31 0.0 0.0 0.0 0.0019520737269616258 0.0 32-33 0.0 0.0 0.0 0.00543977878579973 0.0 34-35 0.0 0.0 0.0 0.010176811029893276 0.0 36-37 0.0 0.0 0.0 0.01814127183589671 0.0 38-39 0.0 0.0 0.0 0.03318525335834764 0.0 40-41 0.0 0.0 0.0 0.05236763118195722 0.0 42-43 0.0 0.0 0.0 0.07115959426017447 0.0 44-45 0.0 0.0 0.0 0.09416803658862882 0.0 46-47 0.0 0.0 0.0 0.122460091804726 0.0 48-49 0.0 0.0 0.0 0.15486451567228898 0.0 50-51 0.0 0.0 0.0 0.18781552018340122 0.0 52-53 0.0 0.0 0.0 0.22081857999389914 0.0 54-55 0.0 0.0 0.0 0.2582203126024839 0.0 56-57 0.0 0.0 0.0 0.30512213734894855 0.0 58-59 0.0 0.0 0.0 0.35944184225786735 0.0 60-61 0.0 0.0 0.0 0.4153232061483555 0.0 62-63 0.0 0.0 0.0 0.47456213684921766 0.0 64-65 0.0 0.0 0.0 0.5378874085518528 0.0 66-67 0.0 0.0 0.0 0.6091771410604914 0.0 68-69 0.0 0.0 0.0 0.6948601638492604 0.0 70-71 0.0 0.0 0.0 0.7913967165599359 0.0 72-73 0.0 0.0 0.0 0.8869702462319772 0.0 74-75 5.2055299385643354E-5 0.0 0.0 0.9862657298100919 0.0 76-77 5.2055299385643354E-5 0.0 0.0 1.093265397697282 0.0 78-79 5.2055299385643354E-5 0.0 0.0 1.1235355542900334 0.0 80-81 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 82-83 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 84-85 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 86-87 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 88-89 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 90-91 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 92-93 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 94-95 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 96-97 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 98-99 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 100-101 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 102-103 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 104-105 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 106-107 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 108-109 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 110-111 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 112-113 5.2055299385643354E-5 5.2055299385643354E-5 0.0 1.124186245532354 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 550 0.0 2335.73 108-109 CCGTTCG 70 0.0 1764.6311 108-109 CCGCGCC 460 0.0 1503.7726 108-109 CTCCGCC 330 0.0 1278.4319 110-111 CGTCGTC 90 5.3592885E-8 1004.48236 110-111 CGTGTCG 80 7.4200216E-8 926.4313 108-109 CCGCGCT 55 2.9945411E-5 898.3576 108-109 CAGCGCG 135 6.530172E-10 731.9951 108-109 CCTCGCG 240 0.0 720.5577 108-109 CGCCGCC 340 0.0 709.0463 110-111 CATCGCG 105 2.2001404E-7 705.8525 108-109 CGTCGCC 245 0.0 705.8524 108-109 CCGCACC 245 0.0 705.8524 108-109 TTGTTCG 335 0.0 663.712 108-109 CCGTTCC 335 0.0 663.712 108-109 CCGCTCG 155 1.3005774E-9 637.5441 108-109 CGTCGAT 100 9.899501E-5 602.6894 110-111 CGACGCC 125 4.4162516E-7 592.9161 108-109 CCGCGCG 255 0.0 581.2903 108-109 CGCCGCG 155 1.0430904E-6 478.15808 108-109 >>END_MODULE