FastQCFastQC Report
Fri 27 May 2016
SRR939951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921034
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25130.13081496735612178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65150.037.6713141
TCAACGC73000.032.7184644
ATCAACG73350.032.5623443
GGTATCA44200.032.076181
CAACGCA74650.032.0750735
TATCAAC76650.031.4712682
AACGCAG76200.031.309386
ACGCAGA86200.027.608187
CGCAGAG87050.027.4069468
GTACATG69200.027.2885911
TACATGG68250.026.8793932
ACATGGG70150.024.6230413
GTAGGAC35150.024.062153
TAGGACC15600.023.672194
GAGTACT45500.023.53734412-13
GCAGAGT101400.023.4119
AGAGTAC82850.022.83657310-11
ATTCGCG802.6141066E-422.31086280-81
AGTACTT49400.022.28133212-13
TAGGGCG1903.2805212E-521.9440025
Produced by FastQC (version 0.11.5)