FastQCFastQC Report
Fri 27 May 2016
SRR939950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733774
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39930.14606181783863625No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA37700.13790459635653862No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC29780.10893365728110664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG2350.01786.8997108-109
CCGCGCC5600.01632.0476108-109
CCGTTCG950.01560.0797108-109
CCGCTCC7950.01491.3969108-109
CGACGCC1750.01411.5006108-109
CCGCGCT1450.01362.8281108-109
CCGCGCG2400.01337.985108-109
CGTCGCG1000.01235.063108-109
CTCCGCC4700.0976.92096110-111
CGGCGCC3700.0867.8821108-109
CCGCACG1750.0846.9003108-109
CGTCGTA855.811946E-5720.23975110-111
CGTCGCC3450.0644.38074108-109
CCTCGCG3550.0626.2291108-109
CCGCGAC809.224742E-5617.53156108-109
CGCCGCC5200.0588.6575110-111
TTGTTCG4000.0555.7784108-109
GGTCGCC2351.6370905E-11525.5587108-109
CGCGTTT1255.8646765E-7489.76306110-111
CGCCGTC1251.8465919E-4489.76306110-111
Produced by FastQC (version 0.11.5)