##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939950_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2733774 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03650301744036 34.0 31.0 34.0 30.0 34.0 2 32.16668971173184 34.0 31.0 34.0 30.0 34.0 3 32.269464118101936 34.0 31.0 34.0 30.0 34.0 4 35.58481388732207 37.0 35.0 37.0 35.0 37.0 5 35.5915174407248 37.0 35.0 37.0 35.0 37.0 6 35.7406398626953 37.0 37.0 37.0 35.0 37.0 7 35.73765497806329 37.0 37.0 37.0 35.0 37.0 8 35.7613676185376 37.0 37.0 37.0 35.0 37.0 9 37.50891624545409 39.0 39.0 39.0 35.0 39.0 10-11 37.47718684865684 39.0 39.0 39.0 35.0 39.0 12-13 37.4322934156225 39.0 39.0 39.0 35.0 39.0 14-15 38.98239539918077 41.0 40.0 41.0 36.0 41.0 16-17 38.970979495744714 41.0 40.0 41.0 36.0 41.0 18-19 38.970474881976344 41.0 40.0 41.0 36.0 41.0 20-21 38.99924774322969 41.0 40.0 41.0 36.0 41.0 22-23 38.97429140082538 41.0 40.0 41.0 36.0 41.0 24-25 38.97670546285099 41.0 39.5 41.0 36.0 41.0 26-27 38.92108363749162 41.0 39.0 41.0 36.0 41.0 28-29 38.866277168485766 41.0 39.0 41.0 35.0 41.0 30-31 38.78347112819128 41.0 39.0 41.0 35.0 41.0 32-33 38.685273362026265 41.0 39.0 41.0 35.0 41.0 34-35 38.629827118115834 41.0 39.0 41.0 35.0 41.0 36-37 38.80302248832566 41.0 39.0 41.0 35.0 41.0 38-39 38.773364403933904 41.0 39.0 41.0 35.0 41.0 40-41 38.705545154793334 41.0 39.0 41.0 35.0 41.0 42-43 38.64974555321691 41.0 39.0 41.0 35.0 41.0 44-45 38.56672296246873 41.0 39.0 41.0 34.5 41.0 46-47 38.47833379789259 41.0 39.0 41.0 34.0 41.0 48-49 38.394392879586974 41.0 39.0 41.0 34.0 41.0 50-51 38.284229603471246 41.0 38.5 41.0 34.0 41.0 52-53 38.186045188812244 40.0 38.0 41.0 33.5 41.0 54-55 38.04690201165129 40.0 38.0 41.0 33.0 41.0 56-57 37.87107986980636 40.0 38.0 41.0 33.0 41.0 58-59 37.708734335757086 40.0 37.5 41.0 33.0 41.0 60-61 37.49283828875394 40.0 37.0 41.0 32.5 41.0 62-63 37.25289270437132 40.0 36.5 41.0 32.0 41.0 64-65 36.97309580089649 39.0 36.0 41.0 32.0 41.0 66-67 36.67305966038158 39.0 35.5 41.0 31.0 41.0 68-69 36.28993764663794 39.0 35.0 41.0 31.0 41.0 70-71 35.88536415226716 37.5 35.0 40.0 31.0 41.0 72-73 35.48594159575737 37.0 35.0 39.5 30.5 41.0 74-75 35.06590230209227 37.0 35.0 39.0 30.0 41.0 76-77 34.61019766081615 36.0 35.0 39.0 30.0 40.5 78-79 34.15250638860418 36.0 34.0 37.5 29.5 39.5 80-81 33.7295436638142 35.0 34.0 37.0 29.0 39.0 82-83 33.52591124943028 35.0 34.0 37.0 30.0 39.0 84-85 33.11660565211316 35.0 34.0 36.0 30.0 37.5 86-87 32.51286810833668 35.0 34.0 36.0 29.0 37.0 88-89 16.83248560415016 18.0 17.0 18.5 2.0 19.0 90-91 2.8348080346071036 2.0 2.0 2.0 2.0 2.0 92-93 2.3177524916104986 2.0 2.0 2.0 2.0 2.0 94-95 2.108344362043095 2.0 2.0 2.0 2.0 2.0 96-97 2.0000162778634953 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09043324581203649 1101 2 0.12622471627297216 1101 3 0.13972191123646382 1101 4 0.12192294421905814 1101 5 0.11812369736335171 1101 6 0.07748980575004794 1101 7 0.1580647250356293 1101 8 0.1786761435555988 1101 9 0.2031467278373995 1101 10-11 0.16791676788999155 1101 12-13 0.2185904537702541 1101 14-15 0.2144969052259853 1101 16-17 0.2653322785211074 1101 18-19 0.3170721763853166 1101 20-21 0.29935953188997644 1101 22-23 0.25554581040371005 1101 24-25 0.22789483194502225 1101 26-27 0.2708381053106166 1101 28-29 0.27780613656657493 1101 30-31 0.23227398844534974 1101 32-33 0.21662452518154396 1101 34-35 0.23541070936907715 1101 36-37 0.25207732356336976 1101 38-39 0.2280340555753 1101 40-41 0.20722971535028023 1101 42-43 0.23433135546375183 1101 44-45 0.24614091431074314 1101 46-47 0.27425310682590975 1101 48-49 0.25008592231364446 1101 50-51 0.2134083993168403 1101 52-53 0.15130311640298544 1101 54-55 0.1926359991970159 1101 56-57 0.21985627311099165 1101 58-59 0.16056420167709717 1101 60-61 0.06146943099179225 1101 62-63 0.07053550032156153 1101 64-65 0.14488925535346908 1101 66-67 0.09221787830329475 1101 68-69 0.09925688186382331 1101 70-71 0.08082431164341841 1101 72-73 -0.032226986511716404 1101 74-75 -0.08049877697229135 1101 76-77 -0.08670678674999976 1101 78-79 -0.06474961531284862 1101 80-81 -0.056071315583942294 1101 82-83 -0.014280337608184368 1101 84-85 0.01030490932579653 1101 86-87 0.16666615986049038 1101 88-89 0.7573178992718361 1101 90-91 0.004295334274999618 1101 92-93 -0.1809251500669058 1101 94-95 -0.007059663481178813 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.044628439284881694 1102 2 0.015358751390529335 1102 3 0.04698684806348297 1102 4 0.08883812256117096 1102 5 0.14093136562240716 1102 6 0.1485562804091174 1102 7 0.13442866090792194 1102 8 0.12570670358365987 1102 9 0.18886929811766606 1102 10-11 0.14457523349424406 1102 12-13 0.14568874784299624 1102 14-15 0.14951788612973615 1102 16-17 0.13259204318780604 1102 18-19 0.09022841636647172 1102 20-21 0.1689252652651163 1102 22-23 0.15264369708025782 1102 24-25 0.16139441834933166 1102 26-27 0.15443739750401875 1102 28-29 0.18035062688736758 1102 30-31 0.20017938750763165 1102 32-33 0.22760668654231608 1102 34-35 0.17131839693516326 1102 36-37 0.21598538664246547 1102 38-39 0.17310748431018652 1102 40-41 0.17512232571479558 1102 42-43 0.17618274087649155 1102 44-45 0.13343114512724696 1102 46-47 0.21251331254327965 1102 48-49 0.07742854782266306 1102 50-51 0.1138735749134625 1102 52-53 0.14881158758679192 1102 54-55 0.10354536063006492 1102 56-57 0.11194541848234962 1102 58-59 0.061552119723153 1102 60-61 0.10562288827984645 1102 62-63 0.09984790411849076 1102 64-65 0.1367785286118064 1102 66-67 0.21031137041486403 1102 68-69 0.20597962750159127 1102 70-71 0.2148789133605149 1102 72-73 0.201709789142285 1102 74-75 0.18229574833144113 1102 76-77 0.2258851209446533 1102 78-79 0.22980859894565953 1102 80-81 0.24048951663790774 1102 82-83 0.2225156507792576 1102 84-85 0.14658077676970294 1102 86-87 0.2165489183223812 1102 88-89 1.1662259534944965 1102 90-91 0.20009394593963803 1102 92-93 0.03334600929408449 1102 94-95 0.13141961510873434 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.008308717837259394 1103 2 0.13465205755450427 1103 3 0.1514274039264123 1103 4 0.05043519328188495 1103 5 0.10650617973087151 1103 6 0.04222351912354583 1103 7 0.01386440401729061 1103 8 0.11440569345025153 1103 9 0.1099881130611422 1103 10-11 0.11959500364850584 1103 12-13 0.10611441250841835 1103 14-15 0.1576249343790579 1103 16-17 0.24302457146688994 1103 18-19 0.16427344372806374 1103 20-21 0.18787815949302455 1103 22-23 0.21040773940672608 1103 24-25 0.14791062144622913 1103 26-27 0.1997675736436051 1103 28-29 0.13817671929675868 1103 30-31 0.12381834457005425 1103 32-33 0.19417920896454177 1103 34-35 0.17635312997280295 1103 36-37 0.20082523006023933 1103 38-39 0.11917956123145501 1103 40-41 0.0788315829032058 1103 42-43 0.15399928796276185 1103 44-45 0.13422914729824953 1103 46-47 0.098367613822262 1103 48-49 0.0015545027747450035 1103 50-51 -0.01712149006950625 1103 52-53 0.01089928161066922 1103 54-55 0.03238493567128842 1103 56-57 0.03210003791365068 1103 58-59 0.03668503841608839 1103 60-61 0.04243816743086626 1103 62-63 -0.02780684604014283 1103 64-65 -0.07640949211733528 1103 66-67 -0.012704193711471135 1103 68-69 -0.061862255673887034 1103 70-71 -0.09554406822478967 1103 72-73 -0.0472405714081674 1103 74-75 -0.031831281908687004 1103 76-77 0.010377383839355048 1103 78-79 -0.005194405213146069 1103 80-81 -0.030963011107985494 1103 82-83 0.036740176543986536 1103 84-85 0.06677565953623343 1103 86-87 0.016671545021502254 1103 88-89 0.8745416309269984 1103 90-91 -0.6047753681835268 1103 92-93 -0.3469654185232818 1103 94-95 -0.12115362321272238 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.17966920954817311 1104 2 -0.02861257665465189 1104 3 -0.025170486616985954 1104 4 -0.01803910680960996 1104 5 -0.06622808205224828 1104 6 0.033249540038625014 1104 7 0.06804056945171055 1104 8 0.08272488019111535 1104 9 0.08078215694212787 1104 10-11 0.0822609300617998 1104 12-13 0.11521265220288512 1104 14-15 0.028371165804351506 1104 16-17 0.09965949060987356 1104 18-19 0.1387624153334528 1104 20-21 0.07438355022626553 1104 22-23 -0.038555560700167746 1104 24-25 0.018447528174831973 1104 26-27 0.09505922497682917 1104 28-29 0.11612046110233365 1104 30-31 0.09099233570931631 1104 32-33 0.11614776120630665 1104 34-35 0.15344872010183508 1104 36-37 0.09169265275182426 1104 38-39 0.040978051677463156 1104 40-41 0.1143505687560804 1104 42-43 0.08185262884732225 1104 44-45 0.0802242716624022 1104 46-47 0.057235360706592076 1104 48-49 0.1264052838376415 1104 50-51 0.08677199575389949 1104 52-53 0.08072358826036208 1104 54-55 0.06675709964855514 1104 56-57 0.053541166263705975 1104 58-59 0.032731101664744244 1104 60-61 0.012947347847180879 1104 62-63 0.02895310370334414 1104 64-65 -0.0395664205225188 1104 66-67 0.011491975556971568 1104 68-69 0.08739096594172224 1104 70-71 0.12176185024431874 1104 72-73 0.09837981959854858 1104 74-75 -0.038042387408601996 1104 76-77 -0.03306251889750911 1104 78-79 0.09442871740287018 1104 80-81 0.05599213399813863 1104 82-83 0.06937508161472294 1104 84-85 0.02659417930561858 1104 86-87 0.1239553474917372 1104 88-89 0.8162688812786705 1104 90-91 -0.6640559098748202 1104 92-93 -0.3469654185232818 1104 94-95 -0.12115362321272238 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.0967583441623816 1105 2 -0.14406725012979393 1105 3 -0.07200932427908668 1105 4 0.040942622982342414 1105 5 0.002964820486234032 1105 6 0.02555327612473235 1105 7 0.0713606164742302 1105 8 0.006167871509092038 1105 9 0.03477447904639064 1105 10-11 0.008049689965304196 1105 12-13 0.0569136061232669 1105 14-15 -0.06498965855919181 1105 16-17 0.047008657307152646 1105 18-19 0.0508385697730418 1105 20-21 0.07127091047509282 1105 22-23 -0.05205616684983738 1105 24-25 0.08940607121146371 1105 26-27 -0.0027298594310352087 1105 28-29 -0.07078486934107531 1105 30-31 -0.02598536533032103 1105 32-33 -0.09722227785667314 1105 34-35 0.017433174505910642 1105 36-37 -0.033801757201281646 1105 38-39 -0.04814814378437404 1105 40-41 0.042165950263012064 1105 42-43 0.027124661469194677 1105 44-45 -0.028659953751791534 1105 46-47 0.0785516238857582 1105 48-49 0.09991455364158952 1105 50-51 0.05906513952769643 1105 52-53 0.018938416273051928 1105 54-55 -0.034293865904999166 1105 56-57 0.010104267055986327 1105 58-59 -0.09512897572648882 1105 60-61 -0.1212581675024964 1105 62-63 0.011634979216125885 1105 64-65 0.051154744509389616 1105 66-67 0.0826456804171869 1105 68-69 0.06891488130119683 1105 70-71 -0.03605518439804456 1105 72-73 0.008756918162163174 1105 74-75 -0.06018139160878633 1105 76-77 -0.009992095327476136 1105 78-79 -0.017387527408125436 1105 80-81 -0.05996460283493832 1105 82-83 -0.04376137553233406 1105 84-85 -0.03953353459174025 1105 86-87 -0.027027961962978964 1105 88-89 1.0034244780605661 1105 90-91 -0.8491274845169468 1105 92-93 -0.3469654185232818 1105 94-95 -0.12115362321272238 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.09617396229787545 1106 2 -0.03897053299495212 1106 3 0.022787962153223873 1106 4 0.052271775468199166 1106 5 -0.011188875079028548 1106 6 0.02127395597715065 1106 7 -0.05069995565571617 1106 8 0.044952460498151936 1106 9 0.0271009864159808 1106 10-11 -0.03786559328932526 1106 12-13 -0.0437278677656181 1106 14-15 0.012031557692708361 1106 16-17 -0.024087140749351477 1106 18-19 -0.07285482318973635 1106 20-21 -0.03037437618735339 1106 22-23 -0.05690530080661205 1106 24-25 -0.16242221973067217 1106 26-27 -0.24873692143415127 1106 28-29 -0.0626544192287426 1106 30-31 -0.0240412675687125 1106 32-33 0.01674159041564849 1106 34-35 -0.02365667093255297 1106 36-37 0.042865215578956395 1106 38-39 0.001665938324869387 1106 40-41 0.049256167463497036 1106 42-43 -0.26595575198328447 1106 44-45 0.014486937978418268 1106 46-47 -0.048329868119154185 1106 48-49 0.009850502143699202 1106 50-51 -0.06294472101728843 1106 52-53 -0.1673717977409055 1106 54-55 -0.11109305761765143 1106 56-57 -0.23918918226119956 1106 58-59 -0.09419514494016568 1106 60-61 0.0326882818014127 1106 62-63 -0.02462491046497206 1106 64-65 0.00683825922422443 1106 66-67 -0.02368434246387352 1106 68-69 0.0715076263358867 1106 70-71 0.03194213581739547 1106 72-73 0.04502446068244126 1106 74-75 -0.017697346395443958 1106 76-77 -0.010775266605023148 1106 78-79 0.02877356872923542 1106 80-81 0.09578044808317543 1106 82-83 0.09567778015481565 1106 84-85 0.038477848407232784 1106 86-87 0.15521370769106824 1106 88-89 0.5445067628627278 1106 90-91 -0.36969905386900415 1106 92-93 -0.31826376787041166 1106 94-95 -0.12115362321272238 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.005117143490387832 1107 2 -0.043082707417596566 1107 3 0.029514397918944724 1107 4 0.08408110884204945 1107 5 0.08725167125562905 1107 6 0.07529507202696806 1107 7 0.024729541479970862 1107 8 0.05945839578770773 1107 9 0.13186878471867658 1107 10-11 0.11988916474962252 1107 12-13 0.12040235472676386 1107 14-15 0.15267981276331 1107 16-17 0.12577620127540712 1107 18-19 0.10954112938259897 1107 20-21 0.0589292898972289 1107 22-23 0.17969026487926243 1107 24-25 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2216 92-93 -0.3469654185232818 2216 94-95 -0.12115362321272238 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19287.0 3 3326.0 4 2560.0 5 2160.0 6 2387.0 7 2655.0 8 3087.0 9 3680.0 10 4458.0 11 5504.0 12 6507.0 13 7097.0 14 8047.0 15 8496.0 16 10039.0 17 12103.0 18 15613.0 19 19316.0 20 22513.0 21 30955.0 22 38527.0 23 50699.0 24 77618.0 25 132076.0 26 261365.0 27 808519.0 28 1132535.0 29 41133.0 30 1512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.331068332641976 19.96251336065088 14.572199457599641 24.134218849107498 2 13.575859156464896 20.479508602995804 43.17925843445485 22.765373806084447 3 18.475220336055482 25.518618597958607 29.717043273840282 26.28911779214563 4 11.699857127800353 17.689803375062947 40.01204752257264 30.598291974564063 5 12.781754842591308 38.629475936267255 35.87859788550518 12.710171335636252 6 30.10545129187709 38.018139026854456 19.192003435543683 12.684406245724775 7 27.346005924410722 32.17471524712723 22.91272065649904 17.56655817196301 8 23.93186122920183 37.81066028135464 21.24941564299024 17.008062846453292 9 25.33087958258437 17.37916155468594 21.500460535508786 35.789498327220905 10-11 23.705748783037237 26.70503273430934 30.123465822162025 19.465752660491393 12-13 25.668762280631324 24.28184541571566 29.126241826401372 20.92315047725165 14-15 20.89210056798801 28.025704406639544 26.273162818047226 24.809032207325227 16-17 20.83606308515911 29.563459185409137 28.843170499293098 20.757307230138654 18-19 21.499426024467674 28.246180995812797 29.544174166638008 20.71021881308152 20-21 22.30864705137838 27.971063584466282 28.314990792759165 21.405298571396173 22-23 21.502855686984443 29.18519962739901 28.25742828866737 21.05451639694918 24-25 21.96999459355455 28.112803033462164 29.026027755037543 20.89117461794574 26-27 20.823392862760418 29.064820281413166 28.702482355893356 21.40930449993306 28-29 21.12630254177288 29.027543386966176 27.73528513943253 22.110868931828414 30-31 21.783841678207487 28.108175730693176 28.322147331856982 21.78583525924235 32-33 20.951967424828673 28.977607038859055 28.683595742606688 21.38682979370558 34-35 21.441510118619988 28.984165468078537 28.59554491010955 20.978779503191927 36-37 21.16612913078674 28.323861069562884 28.602889561707983 21.907120237942387 38-39 21.74325675787391 28.94429093260818 27.915804305696078 21.396648003821824 40-41 22.757056728171385 27.807254732834537 28.57695990963408 20.858728629359998 42-43 21.211153518908294 28.965598473026667 28.54989110292219 21.273356905142855 44-45 21.10650554353816 28.993786416077295 28.129009460749877 21.77069857963467 46-47 22.215113612171304 27.975940951958723 28.00902708124373 21.79991835462624 48-49 21.568241214191612 28.29474101602202 28.077275717649204 22.059742052137167 50-51 21.099229068423995 29.13936996540126 28.264515986254153 21.496884979920587 52-53 21.248098782123176 28.79091322106363 28.402347816608103 21.558640180205092 54-55 21.22181272805421 28.1832847122516 28.725914274478964 21.86898828521523 56-57 21.616033366328015 28.275892593901325 27.644768733626112 22.463305306144544 58-59 21.985284811399918 28.348868633617847 27.370495878591285 22.295350676390953 60-61 21.329835604552535 27.828489114315964 28.97622480863451 21.865450472496995 62-63 21.574812251271 27.90249448244341 28.76558720025635 21.757106066029245 64-65 21.619089232585825 28.4403250235069 27.865843944894443 22.074741799012827 66-67 21.86592600558788 28.833473432697804 27.286527708581616 22.014072853132703 68-69 22.227056824562464 27.676080423884276 28.548393881697955 21.54846886985531 70-71 22.643239711841577 27.669533033820642 28.00635678004107 21.680870474296704 72-73 22.00280637682559 28.34956364351991 27.782362404500155 21.865267575154345 74-75 21.279227909841854 28.934396186370932 28.38347281084684 21.40290309294038 76-77 21.787371596920593 27.735906479467577 28.646607217714415 21.830114705897415 78-79 21.65124167494889 27.85250169454822 28.21996168656656 22.276294943936335 80-81 21.969555639932196 28.331292198989384 28.00628362110401 21.69286853997441 82-83 21.574481277143274 28.31436219196472 28.728572303871136 21.382584227020867 84-85 21.56899611471104 28.070193086588922 28.681801912265225 21.67900888643481 86-87 21.015947731736304 28.984088848147028 27.718991873047322 22.28097154706935 88-89 30.770617622106244 17.72343198532455 34.261615891540636 17.244334501028565 90-91 31.183953800410467 14.83684951192305 16.916287558503733 37.06290912916275 92-93 23.185690768949165 17.34145269834184 35.04083853364538 24.43201799906362 94-95 15.993540747690924 6.9389916065501644 27.700703639723738 49.366764006035176 96-97 27.801296755844447 7.19188594666611 15.075131893728761 49.93168540376068 98-99 57.53452706906464 14.161807981981084 18.74975389372934 9.553911055224933 100-101 31.156869591476593 7.02935819641964 12.515783215529364 49.297988996574404 102-103 23.848877474675362 2.0454836675612906 36.64971282062067 37.455926037142675 104-105 42.88254596517779 4.553663026316747 39.56526800590799 12.998523002597478 106-107 5.752868441350315 1.7163689988530233 6.606307174000792 85.92445538579587 108-109 19.143856483325912 10.705183681121671 21.312881448424324 48.83807838712809 110-111 40.766609091849475 2.688114488887178 24.840957586284045 31.704318832979304 112-113 28.04100676373917 2.786361906706337 38.35988943074814 30.812741898806355 114-115 28.920657214971595 3.931886607471892 23.19165061639978 43.95580556115673 116-117 19.97352801409366 4.206437842936885 20.106979121939723 55.71305502102973 118-119 20.75041667178681 4.315083421669262 15.54798564980426 59.38651425673967 120-121 25.938899071962528 4.278165107506531 29.784837765922735 39.998098054608214 122-123 34.5921566728507 1.6359432360533985 33.914006676331105 29.857893414764803 124-125 38.86063852358174 1.8406659920662019 19.352646480020713 39.94604900433135 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 30.5 2 66.5 3 111.0 4 141.0 5 162.5 6 168.0 7 147.0 8 122.5 9 117.0 10 124.5 11 116.0 12 100.5 13 120.0 14 149.5 15 179.5 16 224.5 17 287.5 18 424.0 19 640.5 20 945.0 21 1470.5 22 2395.5 23 3952.0 24 6136.5 25 9170.5 26 13249.5 27 18471.0 28 24854.0 29 32681.5 30 42599.5 31 54101.0 32 67284.0 33 82055.5 34 95928.0 35 107146.0 36 114411.0 37 117472.0 38 118430.5 39 118188.0 40 117910.0 41 117267.0 42 115555.0 43 114056.0 44 112927.0 45 110213.0 46 105968.0 47 101768.5 48 97157.5 49 92150.5 50 86783.0 51 80547.0 52 73290.5 53 64909.5 54 56551.0 55 48919.5 56 41866.5 57 35391.0 58 29393.5 59 23895.5 60 18582.5 61 14298.5 62 11154.5 63 8415.0 64 6084.5 65 4325.0 66 3004.0 67 2066.5 68 1466.5 69 1050.0 70 724.0 71 510.5 72 348.0 73 213.0 74 150.0 75 112.5 76 83.0 77 56.5 78 36.5 79 23.5 80 17.5 81 17.0 82 14.0 83 9.0 84 7.0 85 5.5 86 5.5 87 6.5 88 8.5 89 9.5 90 11.0 91 9.5 92 5.5 93 3.5 94 4.0 95 4.0 96 6.0 97 10.5 98 10.0 99 4.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.218509650029592E-4 3 0.041334799438432 4 0.04616328928433733 5 0.04758988855699118 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.864585733860956E-4 12-13 0.0017741042236849131 14-15 5.486920279437876E-5 16-17 3.292152167662726E-4 18-19 0.007608529454153855 20-21 0.002048450237656807 22-23 6.76720167797338E-4 24-25 0.0 26-27 0.0 28-29 0.0011888327272115398 30-31 0.0 32-33 0.0012985711328002973 34-35 7.315893705917168E-5 36-37 1.0973840558875752E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.0118707691272212E-4 46-47 0.0 48-49 1.646076083831363E-4 50-51 1.2802813985355043E-4 52-53 0.0 54-55 0.0017923939579497063 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.828973426479292E-5 64-65 9.144867132396461E-5 66-67 0.0 68-69 3.292152167662726E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.5605627970710085E-4 80-81 0.0 82-83 6.035612307381664E-4 84-85 1.828973426479292E-5 86-87 0.0011339635244171612 88-89 19.350666880290763 90-91 26.28300656894096 92-93 40.3479768261751 94-95 44.79598533016994 96-97 48.34187098128814 98-99 93.03283665730963 100-101 51.547293229067215 102-103 53.04002818082255 104-105 74.86240632912596 106-107 81.69395129224289 108-109 12.590579908946387 110-111 39.17697658987173 112-113 51.98353997075106 114-115 86.84794353885874 116-117 66.47709357101209 118-119 71.72076038472822 120-121 80.38268708386282 122-123 75.82675085797143 124-125 49.91519050221416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2733774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.13134881623074 #Duplication Level Percentage of deduplicated Percentage of total 1 72.1618559117578 40.5054230540946 2 14.969727427321928 16.80541983813807 3 5.545223356043021 9.337825993858816 4 2.665351523354688 5.984391043011759 5 1.4675499195122763 4.118777821868747 6 0.8714185592285958 2.934833946779852 7 0.5492586002692801 2.1581438257420724 8 0.3741347139820305 1.6800548907828863 9 0.2699955431415918 1.363969260981753 >10 1.0675077347733837 9.60582399781759 >50 0.03565779518698741 1.386117208295377 >100 0.018731158757816824 2.03183884775784 >500 0.002674509518120239 1.0653665514155874 >1k 9.132471524510514E-4 1.0220137194550956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3993 0.14606181783863625 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3770 0.13790459635653862 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2978 0.10893365728110664 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.657946852958584E-5 2 0.0 0.0 0.0 3.657946852958584E-5 3.657946852958584E-5 3 0.0 0.0 0.0 7.315893705917168E-5 3.657946852958584E-5 4 0.0 0.0 0.0 7.315893705917168E-5 3.657946852958584E-5 5 0.0 0.0 0.0 7.315893705917168E-5 3.657946852958584E-5 6 0.0 0.0 0.0 1.4631787411834336E-4 3.657946852958584E-5 7 0.0 0.0 0.0 1.828973426479292E-4 3.657946852958584E-5 8 7.315893705917168E-5 0.0 0.0 1.828973426479292E-4 3.657946852958584E-5 9 7.315893705917168E-5 0.0 0.0 2.1947681117751505E-4 3.657946852958584E-5 10-11 1.4631787411834336E-4 0.0 0.0 2.743460139718938E-4 5.486920279437876E-5 12-13 1.4631787411834336E-4 0.0 0.0 3.8408441956065134E-4 2.0118707691272212E-4 14-15 1.4631787411834336E-4 0.0 0.0 4.2066388809023714E-4 2.3776654544230795E-4 16-17 1.4631787411834336E-4 0.0 0.0 6.95009902062131E-4 2.5605627970710085E-4 18-19 1.4631787411834336E-4 0.0 0.0 9.14486713239646E-4 3.657946852958584E-4 20-21 1.646076083831363E-4 0.0 0.0 0.0010242251188284034 4.57243356619823E-4 22-23 2.1947681117751502E-4 0.0 0.0 0.0011156737901523682 4.755330908846159E-4 24-25 2.5605627970710085E-4 1.828973426479292E-5 0.0 0.0014083095383890546 4.755330908846159E-4 26-27 2.5605627970710085E-4 3.657946852958584E-5 0.0 0.0016826555523609486 4.938228251494088E-4 28-29 2.5605627970710085E-4 3.657946852958584E-5 0.0 0.0026154319998653876 5.121125594142017E-4 30-31 2.926357482366867E-4 3.657946852958584E-5 0.0 0.005340602405319533 5.121125594142017E-4 32-33 2.926357482366867E-4 3.657946852958584E-5 0.0 0.011229896838582852 5.852714964733734E-4 34-35 2.926357482366867E-4 3.657946852958584E-5 0.0 0.01909448257244381 5.852714964733734E-4 36-37 2.926357482366867E-4 3.657946852958584E-5 0.0 0.03268375513118495 5.852714964733734E-4 38-39 3.657946852958584E-4 3.657946852958584E-5 0.0 0.05611290472438468 6.584304335325451E-4 40-41 4.755330908846159E-4 3.657946852958584E-5 0.0 0.08329144984186695 6.584304335325451E-4 42-43 4.755330908846159E-4 3.657946852958584E-5 0.0 0.1126281836025948 6.584304335325451E-4 44-45 4.938228251494088E-4 3.657946852958584E-5 0.0 0.14715920189452383 6.95009902062131E-4 46-47 5.669817622085806E-4 3.657946852958584E-5 0.0 0.1877624119623641 7.315893705917168E-4 48-49 6.218509650029592E-4 3.657946852958584E-5 0.0 0.2330295042677266 7.864585733860956E-4 50-51 6.767201677973381E-4 3.657946852958584E-5 0.0 0.28157045900648703 8.779072447100602E-4 52-53 7.498791048565097E-4 3.657946852958584E-5 0.0 0.33444608076600335 9.14486713239646E-4 54-55 8.047483076508885E-4 3.657946852958584E-5 0.0 0.3915832106092164 9.14486713239646E-4 56-57 8.047483076508885E-4 3.657946852958584E-5 0.0 0.46150486470351976 9.327764475044388E-4 58-59 8.779072447100602E-4 3.657946852958584E-5 0.0 0.537260944028292 9.510661817692318E-4 60-61 9.14486713239646E-4 3.657946852958584E-5 0.0 0.621174244835162 9.693559160340248E-4 62-63 9.14486713239646E-4 3.657946852958584E-5 0.0 0.7077761365789563 0.0010608045873579893 64-65 9.14486713239646E-4 3.657946852958584E-5 0.0 0.7947621127423116 0.0010608045873579893 66-67 9.510661817692318E-4 3.657946852958584E-5 0.0 0.898940439114572 0.0010608045873579893 68-69 9.510661817692318E-4 3.657946852958584E-5 0.0 1.0259077743807645 0.0010608045873579893 70-71 9.510661817692318E-4 3.657946852958584E-5 0.0 1.157977945506834 0.0011339635244171612 72-73 9.510661817692318E-4 3.657946852958584E-5 0.0 1.2890238915140753 0.0012985711328002973 74-75 9.876456502988177E-4 3.657946852958584E-5 0.0 1.429324443059302 0.0014083095383890549 76-77 0.0010425148530931964 3.657946852958584E-5 0.0 1.5875489341840257 0.0014265992726538478 78-79 0.0010608045873579893 3.657946852958584E-5 0.0 1.6299628279440803 0.0014265992726538478 80-81 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306212583776127 0.0014265992726538478 82-83 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 84-85 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 86-87 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 88-89 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 90-91 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 92-93 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 94-95 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 96-97 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 98-99 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 100-101 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 102-103 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 104-105 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 106-107 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 108-109 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 110-111 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 112-113 0.0010608045873579893 3.657946852958584E-5 0.0 1.6306395481118776 0.0014265992726538478 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCG 235 0.0 1786.8997 108-109 CCGCGCC 560 0.0 1632.0476 108-109 CCGTTCG 95 0.0 1560.0797 108-109 CCGCTCC 795 0.0 1491.3969 108-109 CGACGCC 175 0.0 1411.5006 108-109 CCGCGCT 145 0.0 1362.8281 108-109 CCGCGCG 240 0.0 1337.985 108-109 CGTCGCG 100 0.0 1235.063 108-109 CTCCGCC 470 0.0 976.92096 110-111 CGGCGCC 370 0.0 867.8821 108-109 CCGCACG 175 0.0 846.9003 108-109 CGTCGTA 85 5.811946E-5 720.23975 110-111 CGTCGCC 345 0.0 644.38074 108-109 CCTCGCG 355 0.0 626.2291 108-109 CCGCGAC 80 9.224742E-5 617.53156 108-109 CGCCGCC 520 0.0 588.6575 110-111 TTGTTCG 400 0.0 555.7784 108-109 GGTCGCC 235 1.6370905E-11 525.5587 108-109 CGCGTTT 125 5.8646765E-7 489.76306 110-111 CGCCGTC 125 1.8465919E-4 489.76306 110-111 >>END_MODULE