FastQCFastQC Report
Fri 27 May 2016
SRR939950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733774
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41240.150853728216012No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA38490.1407943743703759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.13640483814682558No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC29240.106958365980509No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC28300.031.9941484
GGACCGA2803.6379788E-1227.6227046
GTATCAA133500.027.2654781
GTACATG76600.026.2092321
GTAGGAC90250.026.0053313
TACATGG77300.025.4300862
GTCCTAC82300.024.5387821
ACATGGG74800.024.5280193
AGAGTAC104200.024.43750810-11
ATCAACG148250.024.1888473
TCAACGC148250.024.1888474
CAACGCA148700.024.1557045
TGTAGGA101700.024.0732252
GGATACG1250.001622399823.8294031
AACGCAG151900.023.5786846
CTACAGT89300.023.3469414
TATCAAC155200.023.3359382
TCCTACA94200.022.9545652
TACAGTG95250.022.8266015
GAGTACT68600.022.59250812-13
Produced by FastQC (version 0.11.5)