FastQCFastQC Report
Fri 27 May 2016
SRR939949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888562
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG1200.02298.8303108-109
CCGCGCA352.562956E-92149.5554108-109
CCGCTCC4950.02077.18108-109
CGTCGCG750.02006.2518108-109
CGTCGCT751.2732926E-111609.493110-111
CATCGCG1100.01367.8989108-109
CGCCGTT458.993269E-61341.2441110-111
CCGCGCC4750.01214.3103108-109
CCGCACG451.5682512E-51114.5844108-109
CCGCGCT704.096364E-81074.7777108-109
CGCGTTT854.2391548E-81065.1057110-111
CGTCGTC602.1309479E-51005.9331110-111
CGTCGCC2800.0985.2129108-109
CCTCGCG2500.0902.8133108-109
CCGCGCG2050.0856.327108-109
CTCCGCC3300.0823.0362110-111
CAGCGCG1254.110916E-10802.5007108-109
CCGTTCG654.723452E-5771.6353108-109
CCACGCG757.2539406E-5668.7506108-109
CGCCGCT1403.1152194E-7646.6713110-111
Produced by FastQC (version 0.11.5)