##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939949_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1888562 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735256242580334 33.0 31.0 34.0 30.0 34.0 2 31.769119573516782 34.0 31.0 34.0 30.0 34.0 3 31.95919858601412 34.0 31.0 34.0 30.0 34.0 4 35.272258469671634 37.0 35.0 37.0 33.0 37.0 5 35.27756250522884 37.0 35.0 37.0 33.0 37.0 6 35.35572885613499 37.0 36.0 37.0 33.0 37.0 7 35.27840441563475 37.0 36.0 37.0 33.0 37.0 8 35.27221134386904 37.0 36.0 37.0 33.0 37.0 9 36.96706859504745 39.0 38.0 39.0 34.0 39.0 10-11 36.97319759690177 39.0 38.0 39.0 34.0 39.0 12-13 36.93842060784873 39.0 38.0 39.0 34.0 39.0 14-15 38.4260037531201 41.0 39.0 41.0 34.0 41.0 16-17 38.39909703785208 41.0 39.0 41.0 34.0 41.0 18-19 38.3863116487571 41.0 39.0 41.0 34.0 41.0 20-21 38.43165779042467 41.0 39.0 41.0 34.0 41.0 22-23 38.38208462311536 41.0 39.0 41.0 34.0 41.0 24-25 38.385372044973906 41.0 39.0 41.0 34.0 41.0 26-27 38.345601574107704 41.0 39.0 41.0 34.0 41.0 28-29 38.28417653219751 41.0 39.0 41.0 34.0 41.0 30-31 38.21284156940572 40.5 39.0 41.0 33.0 41.0 32-33 38.12656216740568 40.0 38.5 41.0 33.0 41.0 34-35 38.05425000608929 40.0 38.0 41.0 33.0 41.0 36-37 38.23510056858075 41.0 39.0 41.0 33.5 41.0 38-39 38.215390334021336 41.0 39.0 41.0 33.0 41.0 40-41 38.16632893174807 41.0 39.0 41.0 33.0 41.0 42-43 38.0901847543263 41.0 38.5 41.0 33.0 41.0 44-45 37.98638911510451 40.5 38.0 41.0 33.0 41.0 46-47 37.89899060766869 40.0 38.0 41.0 33.0 41.0 48-49 37.81666209528731 40.0 38.0 41.0 33.0 41.0 50-51 37.70610231488296 40.0 38.0 41.0 33.0 41.0 52-53 37.59867613559946 40.0 38.0 41.0 32.0 41.0 54-55 37.471045959836104 40.0 38.0 41.0 32.0 41.0 56-57 37.31290685717493 40.0 37.5 41.0 31.5 41.0 58-59 37.166956393277005 40.0 37.0 41.0 31.0 41.0 60-61 36.969153249933015 40.0 37.0 41.0 31.0 41.0 62-63 36.74176807539281 40.0 36.0 41.0 31.0 41.0 64-65 36.47715404630613 39.0 36.0 41.0 30.5 41.0 66-67 36.20592069521678 39.0 35.0 41.0 30.0 41.0 68-69 35.86232302672615 39.0 35.0 40.5 30.0 41.0 70-71 35.49017268164879 38.0 35.0 40.0 29.5 41.0 72-73 35.10457930425371 37.0 35.0 39.5 29.0 41.0 74-75 34.68475856233473 37.0 35.0 39.0 29.0 41.0 76-77 34.230159242852494 36.0 34.0 39.0 29.0 40.5 78-79 33.77786644018042 36.0 34.0 38.5 28.5 39.5 80-81 33.335179623438364 35.0 34.0 37.0 28.0 39.0 82-83 33.11683624895555 35.0 34.0 37.0 29.0 39.0 84-85 32.694818332678516 35.0 34.0 36.5 28.0 38.0 86-87 32.019353878771255 35.0 34.0 36.0 14.5 37.0 88-89 16.388615782801942 18.0 16.5 18.5 2.0 19.0 90-91 2.7526308376426085 2.0 2.0 2.0 2.0 2.0 92-93 2.2905197181771104 2.0 2.0 2.0 2.0 2.0 94-95 2.1003832545608776 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21678231653210744 1101 2 0.16152772852740682 1101 3 0.24233643579882624 1101 4 0.22644073065062287 1101 5 0.23274135249960182 1101 6 0.1330237563621992 1101 7 0.15507967237321907 1101 8 0.11694414199172343 1101 9 0.271091783627881 1101 10-11 0.2362439600346704 1101 12-13 0.21656494542860116 1101 14-15 0.25109267727907536 1101 16-17 0.32785738425239686 1101 18-19 0.3110951717885655 1101 20-21 0.33290728387946444 1101 22-23 0.36418979887884007 1101 24-25 0.35099463489261495 1101 26-27 0.3314883844082175 1101 28-29 0.41507429063393175 1101 30-31 0.2769520396975196 1101 32-33 0.39788065118931115 1101 34-35 0.3454442331727776 1101 36-37 0.3623290616490422 1101 38-39 0.3775999056672035 1101 40-41 0.3655945827184084 1101 42-43 0.33143558021018293 1101 44-45 0.3217602601328906 1101 46-47 0.2801353231485848 1101 48-49 0.30380772204964757 1101 50-51 0.23215066284109298 1101 52-53 0.25109372213724157 1101 54-55 0.29812680357917287 1101 56-57 0.28410720028059444 1101 58-59 0.17943304531450366 1101 60-61 0.15398420612446984 1101 62-63 0.16286063057679456 1101 64-65 0.15960111335004967 1101 66-67 0.15123738116023588 1101 68-69 0.1306242428002662 1101 70-71 0.12386981242115525 1101 72-73 0.1041354928356455 1101 74-75 0.16364315641448002 1101 76-77 0.15793940948280039 1101 78-79 0.139376376700433 1101 80-81 0.16802103444846495 1101 82-83 0.20433493914654832 1101 84-85 0.27789448746319323 1101 86-87 0.39281673079546664 1101 88-89 0.9037325053993008 1101 90-91 -0.03605208561274598 1101 92-93 -0.18012631455559358 1101 94-95 -0.013724226553341268 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.041299609318475206 1102 2 -0.009096090743778262 1102 3 0.009127966812286559 1102 4 0.03529825709683365 1102 5 -0.045197103793881865 1102 6 -0.07956068203704092 1102 7 -0.008260925538692732 1102 8 -0.0013545660215186217 1102 9 0.007026512305031929 1102 10-11 0.1234018568475932 1102 12-13 0.10197870943357401 1102 14-15 0.07107951932873391 1102 16-17 0.11368913154085192 1102 18-19 0.09780662808064733 1102 20-21 0.05553142153360113 1102 22-23 0.03857222752090195 1102 24-25 0.06363169100549726 1102 26-27 0.06656094331962947 1102 28-29 0.05415791123979119 1102 30-31 0.07538132698459776 1102 32-33 0.06253521272949314 1102 34-35 0.05183936332237238 1102 36-37 0.1549413092495513 1102 38-39 0.09331182262842219 1102 40-41 0.09425947339049401 1102 42-43 0.061417956445858124 1102 44-45 0.04642444360036535 1102 46-47 0.11382122568677033 1102 48-49 -0.002381797516115114 1102 50-51 0.06706987668878384 1102 52-53 0.032146484954623133 1102 54-55 0.06469384643549603 1102 56-57 0.0748274642285125 1102 58-59 0.013838901870300901 1102 60-61 -0.025833625293167017 1102 62-63 0.07272275385298599 1102 64-65 0.02471733017165434 1102 66-67 0.05197381783513322 1102 68-69 -0.04293912456435578 1102 70-71 0.0249089946559522 1102 72-73 0.11670478691301867 1102 74-75 0.08754547483406583 1102 76-77 0.030719357555383908 1102 78-79 0.01627790479773239 1102 80-81 0.08883017795606207 1102 82-83 0.2135981221026384 1102 84-85 0.2586690173904813 1102 86-87 0.3687452890419394 1102 88-89 1.1780927458488684 1102 90-91 0.11474739370433706 1102 92-93 0.015507542231246063 1102 94-95 0.1173990454090954 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.05398729233574073 1103 2 0.0983892362195391 1103 3 0.0688721789386193 1103 4 0.01902854188119818 1103 5 0.029327773891814957 1103 6 -0.04986625996557592 1103 7 -0.055480274293145726 1103 8 0.030160600841774965 1103 9 0.07717769371777194 1103 10-11 0.08372403054353583 1103 12-13 0.05678593537769672 1103 14-15 0.08224002839405387 1103 16-17 0.11600322123452145 1103 18-19 0.16206918859092667 1103 20-21 0.09158289786599028 1103 22-23 0.1990402782600782 1103 24-25 0.1348510408610082 1103 26-27 0.15477354421138045 1103 28-29 0.13400787217958765 1103 30-31 0.14302359942384868 1103 32-33 0.11135247871376208 1103 34-35 0.1641461749916573 1103 36-37 0.12070410896569683 1103 38-39 0.14485339297504396 1103 40-41 0.1857855160005144 1103 42-43 0.11997322731553339 1103 44-45 0.14366334998085506 1103 46-47 0.13319070764227803 1103 48-49 0.052339917146795756 1103 50-51 0.04288462179002295 1103 52-53 0.06574479361221819 1103 54-55 -0.03458312698540311 1103 56-57 0.02719755672328006 1103 58-59 0.0576056621089478 1103 60-61 0.06169865648859485 1103 62-63 -0.011512670967277927 1103 64-65 -0.05664445865883039 1103 66-67 -0.029087931412149715 1103 68-69 -0.026676222174110364 1103 70-71 0.0578130832870869 1103 72-73 0.01604205727952035 1103 74-75 0.04162820152773605 1103 76-77 -0.048669808648377 1103 78-79 -0.043156151731928105 1103 80-81 0.03811309711548461 1103 82-83 0.1271555483025253 1103 84-85 0.0772427888660232 1103 86-87 0.11117403789889835 1103 88-89 0.9247684999529397 1103 90-91 -0.5278502031162344 1103 92-93 -0.31757878603848333 1103 94-95 -0.10935160297919655 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.13886234299087974 1104 2 -0.017453260065749987 1104 3 0.017574596877292237 1104 4 0.056737308597391234 1104 5 -0.0534916236497196 1104 6 -0.054875310350553264 1104 7 -0.016165401944093105 1104 8 0.011591912472198374 1104 9 0.08488646043396386 1104 10-11 0.1413991622468984 1104 12-13 0.1567115060922717 1104 14-15 0.14532634475229145 1104 16-17 0.1398083002565471 1104 18-19 0.1627294614532815 1104 20-21 0.09176106887774438 1104 22-23 0.010677182217932568 1104 24-25 0.05454873098663171 1104 26-27 0.09742409128688934 1104 28-29 0.11074176384512668 1104 30-31 0.07619987931037997 1104 32-33 0.1058744292743441 1104 34-35 0.07545875097437005 1104 36-37 0.05034357945063306 1104 38-39 0.050441938159423216 1104 40-41 0.15434812575332302 1104 42-43 0.03296755393987638 1104 44-45 0.07444397945539549 1104 46-47 0.09980694430309711 1104 48-49 0.032405025886497185 1104 50-51 -0.008855213411926854 1104 52-53 0.04327484899516776 1104 54-55 -0.08650437512805098 1104 56-57 -0.10090213259185532 1104 58-59 -0.071476736918477 1104 60-61 -0.05668983466363642 1104 62-63 -0.11447984643668718 1104 64-65 -0.23994123023792469 1104 66-67 -0.1834739911176797 1104 68-69 -0.23734257365323685 1104 70-71 -0.2636607786607712 1104 72-73 -0.1900387207834342 1104 74-75 -0.29164547130760354 1104 76-77 -0.2760080499019182 1104 78-79 -0.24448466719862694 1104 80-81 -0.1904919278743833 1104 82-83 -0.16903180817457297 1104 84-85 -0.18136372200795137 1104 86-87 -0.2361912194292124 1104 88-89 0.5589962454615183 1104 90-91 -0.5631222549870976 1104 92-93 -0.31757878603848333 1104 94-95 -0.10935160297919655 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.010375986247449731 1105 2 -0.02786767448016292 1105 3 0.10564339294609226 1105 4 0.11641298427306879 1105 5 0.0918842977262031 1105 6 0.0890755496003024 1105 7 0.1558935661148695 1105 8 0.037937530817814036 1105 9 0.131228802776306 1105 10-11 0.08071283956057584 1105 12-13 0.17559930298006776 1105 14-15 0.07811422354016884 1105 16-17 0.20450281295104844 1105 18-19 0.24827737300118713 1105 20-21 0.26452273963941053 1105 22-23 0.16078692832768127 1105 24-25 0.2342669947049032 1105 26-27 0.25909157695437557 1105 28-29 0.19139532649869295 1105 30-31 0.11692223803273549 1105 32-33 0.13987770527761967 1105 34-35 0.2172408967565147 1105 36-37 0.09628788739493643 1105 38-39 0.16757215928964797 1105 40-41 0.2519173313225238 1105 42-43 0.2184352034075303 1105 44-45 0.13132522433663496 1105 46-47 0.2357856502818052 1105 48-49 0.23488332236478726 1105 50-51 0.25659933204261876 1105 52-53 0.2979922937126176 1105 54-55 0.20102715640347668 1105 56-57 0.22683251114278846 1105 58-59 0.2326379231961866 1105 60-61 0.11306446509065893 1105 62-63 0.1727945188376836 1105 64-65 0.20049447819778266 1105 66-67 0.2613990637553769 1105 68-69 0.30291623060556105 1105 70-71 0.2179379109379198 1105 72-73 0.22933228658757088 1105 74-75 0.12951750985537558 1105 76-77 0.21599031209643726 1105 78-79 0.25258330986934396 1105 80-81 0.12640078301832602 1105 82-83 0.20010824096547708 1105 84-85 0.15951097086674793 1105 86-87 0.2327358894978957 1105 88-89 0.7107112331765073 1105 90-91 -0.7605768004416431 1105 92-93 -0.31757878603848333 1105 94-95 -0.10935160297919655 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.030266816769415783 1106 2 0.0757753514349524 1106 3 0.09036383568896866 1106 4 0.1148467055118374 1106 5 0.0903464485165415 1106 6 0.04794784405464725 1106 7 0.012702633246519213 1106 8 0.16337490367454421 1106 9 0.1270631785265337 1106 10-11 0.053092519380648184 1106 12-13 0.06430184142313777 1106 14-15 0.12719323242282599 1106 16-17 0.06319233697718118 1106 18-19 0.008435441307923952 1106 20-21 0.04129390070229988 1106 22-23 0.011175970946737834 1106 24-25 -0.031015721391277395 1106 26-27 -0.043845448430055 1106 28-29 0.12281737266827975 1106 30-31 0.12315589104058233 1106 32-33 0.19561674726490708 1106 34-35 0.0605560351768446 1106 36-37 0.171620386794757 1106 38-39 0.16863561404875327 1106 40-41 0.17060287521143636 1106 42-43 -0.2927951644076998 1106 44-45 0.013346312746229216 1106 46-47 -0.0243687537716184 1106 48-49 -0.005540530660745446 1106 50-51 -0.13344975631642342 1106 52-53 -0.10256284066439747 1106 54-55 -0.013456689293548152 1106 56-57 -0.12740576640545243 1106 58-59 0.024058389946240766 1106 60-61 0.1848733426919651 1106 62-63 0.21195085610060715 1106 64-65 0.11450235002473619 1106 66-67 0.1429820843636449 1106 68-69 0.16434402586224905 1106 70-71 0.11372364056656892 1106 72-73 0.15540256443905776 1106 74-75 0.012221033856235408 1106 76-77 0.06420424131317759 1106 78-79 0.13433848720656982 1106 80-81 0.08675886901456664 1106 82-83 0.20458798017172342 1106 84-85 0.1603975938150839 1106 86-87 0.21035602014747923 1106 88-89 0.9129994330173012 1106 90-91 -0.22761747365342444 1106 92-93 -0.2925086598112747 1106 94-95 -0.10935160297919655 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.10016699253980477 1107 2 -0.09996262855985094 1107 3 0.006146421998735718 1107 4 -0.0061884374123195585 1107 5 -0.002996134406139106 1107 6 -0.023921805839798083 1107 7 -0.05451099528205816 1107 8 -0.09992231375958482 1107 9 -0.027671485436954413 1107 10-11 -0.0993489293561538 1107 12-13 -0.11207961118739718 1107 14-15 -0.08945228120191473 1107 16-17 -0.10178642562826212 1107 18-19 -0.10218101738087171 1107 20-21 -0.13385863965800127 1107 22-23 0.01848009686054297 1107 24-25 -0.06535689010982537 1107 26-27 -0.12656896908784887 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-0.5723190968512739 2216 68-69 -0.5714030707101401 2216 70-71 -0.6029628279304475 2216 72-73 -0.5037837611148035 2216 74-75 -0.5100425718090236 2216 76-77 -0.43447164266407867 2216 78-79 -0.4120255435884346 2216 80-81 -0.3787163665052944 2216 82-83 -0.4130475047082527 2216 84-85 -0.47384115824078066 2216 86-87 -0.546499154899017 2216 88-89 -1.1620310054650123 2216 90-91 -0.7605768004416431 2216 92-93 -0.31757878603848333 2216 94-95 -0.10935160297919655 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24499.0 3 4110.0 4 2788.0 5 2335.0 6 2416.0 7 2454.0 8 2723.0 9 3213.0 10 3984.0 11 4421.0 12 5445.0 13 5875.0 14 6608.0 15 7354.0 16 8403.0 17 10495.0 18 13302.0 19 16269.0 20 17838.0 21 24044.0 22 29166.0 23 37738.0 24 56158.0 25 91407.0 26 177684.0 27 541953.0 28 758984.0 29 25920.0 30 976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.073419882429064 20.229571494078563 14.830437126236786 23.866571497255585 2 13.292928501344681 20.854378987510543 43.31660271117623 22.536089799968547 3 18.115346701689873 26.089747766158112 29.360434474236065 26.43447105791595 4 11.493655571199884 18.18976394720198 40.13559713486294 30.18098334673519 5 13.004863428301118 38.498105959275605 35.5079530640234 12.98907754839988 6 29.36318744102656 38.20631782276674 20.007074165423216 12.423420570783485 7 27.48673329231447 31.980205044896593 23.023337332849014 17.50972432993992 8 23.786245831484486 37.96581737851339 21.68962416907679 16.55831262092534 9 25.424317549542984 17.80396936928732 21.614858289005074 35.15685479216462 10-11 23.26613809578399 27.738030972414574 29.807977171922502 19.187853759878934 12-13 24.68896311507094 24.866535841255754 29.569922934442914 20.874578109230395 14-15 20.86651113386799 27.771394320122926 26.747017042596433 24.61507750341265 16-17 20.905432030910372 30.057287124508285 28.41052719086583 20.62675365371551 18-19 21.18536836572996 29.08052535356517 29.267796080632586 20.466310200072282 20-21 22.073375466867034 28.482946562108573 28.501744355830056 20.941933615194337 22-23 21.368334408672467 29.184245174145367 28.34619548368154 21.101224933500625 24-25 21.41076120349769 28.692809661530838 28.94244403943318 20.9539850955383 26-27 20.88329639164613 29.313308220752088 28.608856897470137 21.194538490131645 28-29 20.8603966805177 29.255446315223736 28.228996093558674 21.655160910699887 30-31 21.41949800959672 28.675759652052722 28.582064025433105 21.322678312917446 32-33 20.958900446559884 29.12919631134705 28.703101879565178 21.208801362527886 34-35 21.12646672597111 29.31376286694625 28.705045113737448 20.854725293345194 36-37 21.01073807272788 28.83712224204626 28.589499780388287 21.562639904837575 38-39 21.313279627568487 29.111885127414403 28.373704437556192 21.201130807460913 40-41 21.94836600545812 28.433273570049593 28.78454612557067 20.833814298921613 42-43 21.0505400405176 29.1173125372638 28.730986856666608 21.10116056555199 44-45 20.920711345645877 29.22480946476988 28.508629451569355 21.345849738014884 46-47 21.41497075552722 28.675997928582696 28.3628231426874 21.54620817320268 48-49 21.277375354767653 28.7345649595459 28.387051510145294 21.601008175541153 50-51 21.076999895687777 29.17777080009595 28.409858087718636 21.335371216497638 52-53 21.10576724513148 28.965742189030596 28.64152725724652 21.286963308591407 54-55 21.021513005869107 28.64934245223274 28.873087668093522 21.456056873804627 56-57 21.253445743375117 28.834531246525135 28.229520661752165 21.68250234834758 58-59 21.391831456949788 28.861747721282114 28.115677430764784 21.630743391003314 60-61 21.186066435732585 28.453129947547396 28.873264420230843 21.48753919648918 62-63 21.344705654355007 28.3979821684435 28.845968519963865 21.411343657237623 64-65 21.30955227033909 28.696735584199935 28.387134864286917 21.606577281174058 66-67 21.34711489482474 29.129067512742502 28.06966358530988 21.45415400712288 68-69 21.604298943584972 28.426465285400443 28.766989823447215 21.202245947567373 70-71 21.77823656305697 28.42400726055062 28.440315965268816 21.357440211123595 72-73 21.4936814359285 28.67091469594326 28.342728488659624 21.49267537946861 74-75 21.16814274564457 28.945382783302854 28.672503206143084 21.213971264909492 76-77 21.38545094098049 28.371877650826395 28.784175473190714 21.458495935002396 78-79 21.282284535457986 28.376344080327907 28.628838177679384 21.71253320653472 80-81 21.388045507640204 28.796830604449315 28.488236022963502 21.32688786494698 82-83 21.339326987381344 28.680953089481736 28.813303095015076 21.166416828121847 84-85 21.305645225556713 28.524873839095967 28.82841190419899 21.341069031148326 86-87 20.986590728095294 28.94020472914584 28.336565099886474 21.736639442872395 88-89 30.5517588143941 17.96236604605518 34.52892985654546 16.95694528300526 90-91 30.673153374030342 14.357390941004903 16.463678618404625 38.50577706656013 92-93 22.598485792067756 17.184836568744327 35.294790128289534 24.921887510898383 94-95 15.295028337626624 6.821845367732084 26.996451381917925 50.88667491272337 96-97 26.38576418124873 7.105748351305198 14.281834830616514 52.22665263682956 98-99 57.288428192769246 14.210964533697954 18.52718981308197 9.973417460450834 100-101 30.688525166363423 6.926111409809524 12.134191314777663 50.25117210904939 102-103 23.269077550576956 1.9855048189007747 36.521960063557884 38.223457566964385 104-105 42.4633473209033 4.727643740500827 38.942514361639766 13.866494576956104 106-107 5.248948480594097 1.5625045044525614 6.023714321132153 87.1648326938212 108-109 17.858941928234014 10.401260318494398 20.577018319906323 51.16277943336526 110-111 39.99763668969736 2.5348240724007063 24.15446491874383 33.313074319158105 112-113 27.498448890957032 2.7480666533714464 37.86600633738838 31.88747811828314 114-115 28.2583559168925 3.954431396165813 22.47776774064037 45.30944494630131 116-117 19.209353151691406 4.070338408110788 19.379988392375658 57.340320047822146 118-119 19.840505473213497 4.2460848459365454 14.359242434142928 61.554167246707024 120-121 25.183509770719382 4.181978887070598 28.582991896197697 42.05151944601233 122-123 33.988840096410996 1.5700905779157175 33.023629500005505 31.417439825667774 124-125 38.07240838144314 1.7637870934448958 18.925529048438243 41.23827547667372 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13.0 1 38.5 2 89.5 3 125.5 4 145.5 5 158.5 6 149.0 7 128.5 8 118.0 9 110.0 10 109.0 11 117.0 12 115.0 13 123.0 14 157.0 15 199.5 16 270.0 17 358.5 18 504.5 19 766.5 20 1170.0 21 1810.0 22 2737.5 23 4074.0 24 6032.5 25 8601.5 26 11819.0 27 15745.5 28 20735.0 29 26707.0 30 33080.0 31 40141.5 32 48091.5 33 56273.5 34 64051.5 35 70959.5 36 75578.0 37 78135.0 38 80082.5 39 81940.0 40 83175.0 41 83592.5 42 83228.5 43 82153.5 44 81223.0 45 79705.0 46 76810.5 47 73013.5 48 68685.0 49 63875.0 50 58805.5 51 53698.5 52 48097.5 53 41696.0 54 35368.5 55 29811.5 56 24930.0 57 20441.0 58 16340.0 59 12981.5 60 10154.0 61 7689.5 62 5818.5 63 4350.0 64 3143.5 65 2254.5 66 1646.0 67 1201.0 68 886.5 69 631.0 70 425.0 71 293.5 72 229.0 73 182.5 74 135.0 75 91.5 76 56.5 77 37.0 78 23.0 79 16.5 80 10.5 81 10.5 82 9.5 83 5.0 84 2.5 85 3.5 86 7.0 87 8.5 88 5.5 89 3.0 90 4.5 91 6.0 92 3.5 93 2.5 94 4.5 95 6.0 96 6.0 97 5.5 98 5.5 99 2.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 4.76553059947198E-4 3 0.03552967813606331 4 0.04045405975551769 5 0.04230732165531235 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.559785699383975E-4 12-13 0.002118013599765324 14-15 0.0 16-17 2.647516999706655E-4 18-19 0.006327565629298907 20-21 0.0022503894497506566 22-23 5.559785699383975E-4 24-25 0.0 26-27 0.0 28-29 7.677799299149299E-4 30-31 0.0 32-33 7.148295899207969E-4 34-35 1.059006799882662E-4 36-37 7.942550999119965E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.647516999706655E-5 46-47 0.0 48-49 1.059006799882662E-4 50-51 5.29503399941331E-5 52-53 0.0 54-55 0.001588510199823993 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.647516999706655E-5 66-67 0.0 68-69 2.647516999706655E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.588510199823993E-4 80-81 0.0 82-83 4.76553059947198E-4 84-85 7.942550999119965E-5 86-87 9.53106119894396E-4 88-89 19.227909912409547 90-91 26.310785666554764 92-93 40.482758839794506 94-95 44.79829097482635 96-97 48.621596749272726 98-99 93.06811214034806 100-101 51.792342533631405 102-103 53.212867779824016 104-105 74.93349437296737 106-107 81.6326390131751 108-109 12.544782750050038 110-111 39.057971091232375 112-113 52.20808212809535 114-115 86.8113940659613 116-117 66.51722315708989 118-119 72.04325301472761 120-121 80.4404356330372 122-123 75.94439578896535 124-125 50.18344645290968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1888562.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.7294169598645 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44711235294396 55.39800823515632 2 13.172997736916653 18.370909036176254 3 3.948974955823542 8.26079163776047 4 1.468740364330142 4.096576370806391 5 0.7208982331628263 2.5133906742920167 6 0.3727727772758227 1.5595937050771527 7 0.21261695671953232 1.0377959499483613 8 0.14380157448369196 0.8021759957326695 9 0.08658888560931648 0.5434013257867836 >10 0.3915836106233108 4.674709145684384 >50 0.021237384833431218 1.0042634302236575 >100 0.01237174857872517 1.5380257243537585 >500 1.5170934949938045E-4 0.07944531970452035 >1k 1.5170934949938045E-4 0.12091344929729261 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.29503399941331E-5 0.0 5 0.0 0.0 0.0 5.29503399941331E-5 0.0 6 0.0 0.0 0.0 5.29503399941331E-5 0.0 7 0.0 0.0 0.0 5.29503399941331E-5 0.0 8 0.0 0.0 0.0 5.29503399941331E-5 0.0 9 0.0 0.0 0.0 5.29503399941331E-5 0.0 10-11 0.0 0.0 0.0 5.29503399941331E-5 0.0 12-13 0.0 0.0 0.0 5.29503399941331E-5 5.29503399941331E-5 14-15 0.0 0.0 0.0 5.29503399941331E-5 7.942550999119965E-5 16-17 5.29503399941331E-5 0.0 0.0 5.29503399941331E-5 1.059006799882662E-4 18-19 5.29503399941331E-5 0.0 0.0 5.29503399941331E-5 2.118013599765324E-4 20-21 5.29503399941331E-5 0.0 0.0 1.059006799882662E-4 3.7065237995893174E-4 22-23 5.29503399941331E-5 0.0 0.0 1.8532618997946584E-4 3.7065237995893174E-4 24-25 5.29503399941331E-5 0.0 0.0 2.647516999706655E-4 3.7065237995893174E-4 26-27 5.29503399941331E-5 0.0 0.0 5.824537399354642E-4 3.9712754995599827E-4 28-29 5.29503399941331E-5 5.29503399941331E-5 0.0 0.0011384323098738618 4.236027199530648E-4 30-31 5.29503399941331E-5 5.29503399941331E-5 0.0 0.003044644549662653 4.236027199530648E-4 32-33 5.29503399941331E-5 5.29503399941331E-5 0.0 0.0069629697092285035 4.236027199530648E-4 34-35 5.29503399941331E-5 5.29503399941331E-5 0.0 0.011384323098738616 5.29503399941331E-4 36-37 5.29503399941331E-5 5.29503399941331E-5 0.0 0.019353349267855646 5.29503399941331E-4 38-39 5.29503399941331E-5 5.29503399941331E-5 0.0 0.03457657201616891 5.559785699383976E-4 40-41 5.29503399941331E-5 5.29503399941331E-5 0.0 0.05316214135410964 5.824537399354642E-4 42-43 5.29503399941331E-5 5.29503399941331E-5 0.0 0.07235663960198288 5.824537399354642E-4 44-45 5.29503399941331E-5 5.29503399941331E-5 0.0 0.09507233545946599 5.824537399354642E-4 46-47 5.29503399941331E-5 5.29503399941331E-5 0.0 0.123321341846336 5.824537399354642E-4 48-49 5.29503399941331E-5 5.29503399941331E-5 0.0 0.15477384380285106 5.824537399354642E-4 50-51 5.29503399941331E-5 5.29503399941331E-5 0.0 0.18818550833914904 5.824537399354642E-4 52-53 5.29503399941331E-5 5.29503399941331E-5 0.0 0.22342395960524464 6.354040799295972E-4 54-55 5.29503399941331E-5 5.29503399941331E-5 0.0 0.26096575066108496 6.354040799295972E-4 56-57 5.29503399941331E-5 5.29503399941331E-5 0.0 0.30517928455618615 6.354040799295972E-4 58-59 5.29503399941331E-5 5.29503399941331E-5 0.0 0.3573353694504072 6.354040799295972E-4 60-61 5.29503399941331E-5 5.29503399941331E-5 0.0 0.41656032473384513 6.354040799295972E-4 62-63 5.29503399941331E-5 5.29503399941331E-5 0.0 0.4759970813772595 6.354040799295972E-4 64-65 5.29503399941331E-5 5.29503399941331E-5 0.0 0.5368105468605214 6.354040799295972E-4 66-67 5.29503399941331E-5 5.29503399941331E-5 0.0 0.6067314708227742 6.354040799295972E-4 68-69 5.29503399941331E-5 5.29503399941331E-5 0.0 0.6908960362434488 6.354040799295972E-4 70-71 5.29503399941331E-5 5.29503399941331E-5 0.0 0.7873450805427622 6.354040799295972E-4 72-73 5.29503399941331E-5 5.29503399941331E-5 0.0 0.8850384578319377 6.618792499266638E-4 74-75 5.29503399941331E-5 5.29503399941331E-5 0.0 0.9810638994112981 7.148295899207968E-4 76-77 5.29503399941331E-5 5.29503399941331E-5 0.0 1.0895856212292738 7.413047599178635E-4 78-79 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1195819888359502 7.413047599178635E-4 80-81 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 82-83 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 84-85 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 86-87 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 88-89 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 90-91 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 92-93 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 94-95 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 96-97 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 98-99 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 100-101 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 102-103 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 104-105 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 106-107 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 108-109 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 110-111 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 112-113 5.29503399941331E-5 5.29503399941331E-5 0.0 1.1201114922358917 7.413047599178635E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCG 120 0.0 2298.8303 108-109 CCGCGCA 35 2.562956E-9 2149.5554 108-109 CCGCTCC 495 0.0 2077.18 108-109 CGTCGCG 75 0.0 2006.2518 108-109 CGTCGCT 75 1.2732926E-11 1609.493 110-111 CATCGCG 110 0.0 1367.8989 108-109 CGCCGTT 45 8.993269E-6 1341.2441 110-111 CCGCGCC 475 0.0 1214.3103 108-109 CCGCACG 45 1.5682512E-5 1114.5844 108-109 CCGCGCT 70 4.096364E-8 1074.7777 108-109 CGCGTTT 85 4.2391548E-8 1065.1057 110-111 CGTCGTC 60 2.1309479E-5 1005.9331 110-111 CGTCGCC 280 0.0 985.2129 108-109 CCTCGCG 250 0.0 902.8133 108-109 CCGCGCG 205 0.0 856.327 108-109 CTCCGCC 330 0.0 823.0362 110-111 CAGCGCG 125 4.110916E-10 802.5007 108-109 CCGTTCG 65 4.723452E-5 771.6353 108-109 CCACGCG 75 7.2539406E-5 668.7506 108-109 CGCCGCT 140 3.1152194E-7 646.6713 110-111 >>END_MODULE