FastQCFastQC Report
Fri 27 May 2016
SRR939949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888562
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.16679357098151926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.11093096228770884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61750.043.3126451
ATCAACG69200.038.042053
TCAACGC69600.037.7378464
CAACGCA71300.036.8380665
TATCAAC72550.036.4496462
AACGCAG74100.035.5691226
GGTATCA40300.031.9266991
ACGCAGA84400.031.016867
CGCAGAG84450.030.8575928
GTACATG70100.029.0611921
TAGTGCG850.007213907327.9981067
TACATGG69800.027.8169772
GCAGAGT96800.026.7363119
GTCTACG1358.186085E-526.4741971
CATGGGG35550.026.4706884
CGTTCGC450.009534812526.44265446-47
TTCGCGC450.009534812526.44265448-49
ACATGGG70500.026.2735653
GTCCTAC31800.024.3512671
TAGGACG32100.024.3060284
Produced by FastQC (version 0.11.5)