FastQCFastQC Report
Fri 27 May 2016
SRR939948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554672
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18940.3414630628551648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10090.18190930856434073No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9580.17271468543571697No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC9370.16892866414746013No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA7300.13160931144892837No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6420.11574407938385207No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6240.11249891827963192No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5860.10564802261516716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA53.1885886E-45054.862108-109
CCACGCG256.330083E-103032.917108-109
CGCGGCT109.46148E-42933.0679110-111
CCGCGCT100.0012752692527.431108-109
CCGCGCC700.02527.431108-109
CCGCTCG550.02297.6643108-109
CCGCTCC1300.02138.5955108-109
AAGCGTC150.00286898091684.9539108-109
CATCGCG150.00286898091684.9539108-109
CGGGTTG354.5503075E-61676.0388110-111
CTGCGCG354.5503075E-61676.0388110-111
CTTCGCT802.0008883E-111466.5339110-111
CTCCGCC903.45608E-111303.5857110-111
CAGCGCA401.064002E-51263.7155108-109
CGGCGCG200.0050997451263.7155108-109
CCGTCCG204.5239646E-41263.7155108-109
GCGCTCG200.0050997451263.7155108-109
GTTCGGT250.00591143361173.2273110-111
CGTCGCC501.3261211E-51173.2273110-111
CGGTATG250.00591143361173.2273110-111
Produced by FastQC (version 0.11.5)