##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939948_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 554672 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.650822468053192 33.0 31.0 34.0 29.0 34.0 2 31.781499697118296 34.0 31.0 34.0 29.0 34.0 3 31.935273458909048 34.0 31.0 34.0 30.0 34.0 4 35.12134558802319 37.0 35.0 37.0 32.0 37.0 5 35.169181065566676 37.0 35.0 37.0 32.0 37.0 6 35.2276516571956 37.0 37.0 37.0 33.0 37.0 7 35.14416808492226 37.0 37.0 37.0 33.0 37.0 8 35.14718969048374 37.0 37.0 37.0 33.0 37.0 9 36.72784095826002 39.0 38.0 39.0 33.0 39.0 10-11 36.706241706810516 39.0 38.0 39.0 33.0 39.0 12-13 36.6386810583552 39.0 38.0 39.0 33.0 39.0 14-15 38.13031665560908 41.0 39.0 41.0 33.0 41.0 16-17 38.16061113595062 41.0 39.0 41.0 33.0 41.0 18-19 38.19571927193008 41.0 39.0 41.0 33.0 41.0 20-21 38.26233341506332 41.0 39.0 41.0 33.0 41.0 22-23 38.2786827169931 41.0 39.0 41.0 33.0 41.0 24-25 38.31779772550264 41.0 39.0 41.0 33.0 41.0 26-27 38.29481657628292 41.0 39.0 41.0 33.0 41.0 28-29 38.24203853809098 41.0 39.0 41.0 33.0 41.0 30-31 38.15691706089365 41.0 39.0 41.0 33.0 41.0 32-33 38.0587220916145 40.0 38.0 41.0 33.0 41.0 34-35 37.994019889231836 40.0 38.0 41.0 33.0 41.0 36-37 38.15811506620128 41.0 39.0 41.0 33.0 41.0 38-39 38.105699404332654 41.0 38.5 41.0 33.0 41.0 40-41 38.02198055787925 41.0 38.0 41.0 33.0 41.0 42-43 37.9687590143364 41.0 38.0 41.0 33.0 41.0 44-45 37.89504067268584 41.0 38.0 41.0 32.5 41.0 46-47 37.78566792626994 40.5 38.0 41.0 32.0 41.0 48-49 37.690161032105465 40.0 38.0 41.0 32.0 41.0 50-51 37.583207733579485 40.0 38.0 41.0 31.5 41.0 52-53 37.49654480485765 40.0 38.0 41.0 31.5 41.0 54-55 37.35401101912482 40.0 38.0 41.0 31.0 41.0 56-57 37.14470353650445 40.0 37.0 41.0 30.5 41.0 58-59 36.98063810684513 40.0 37.0 41.0 30.5 41.0 60-61 36.763302456226384 40.0 36.0 41.0 30.5 41.0 62-63 36.51704971586811 40.0 36.0 41.0 30.0 41.0 64-65 36.237592667378195 39.0 35.5 41.0 30.0 41.0 66-67 35.912450962009984 39.0 35.0 41.0 29.5 41.0 68-69 35.490912647474545 38.5 35.0 40.5 29.0 41.0 70-71 35.070184721781516 37.5 35.0 40.0 28.5 41.0 72-73 34.67857220122883 37.0 34.0 39.5 28.0 41.0 74-75 34.25198946404362 36.5 34.0 39.0 28.0 41.0 76-77 33.77450457207142 36.0 34.0 39.0 26.5 40.5 78-79 33.2863016341189 36.0 34.0 38.5 26.0 39.5 80-81 32.81654563417659 35.0 33.5 37.0 25.5 39.0 82-83 32.57929010297978 35.0 34.0 37.0 25.5 39.0 84-85 32.09941100325958 35.0 34.0 36.5 23.5 38.5 86-87 31.403323044970723 35.0 34.0 36.0 2.0 37.0 88-89 15.928208022038248 18.0 16.5 18.5 2.0 19.5 90-91 2.7688165258026363 2.0 2.0 2.0 2.0 2.0 92-93 2.3079044913029683 2.0 2.0 2.0 2.0 2.0 94-95 2.1037369832982376 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15009261062567347 1101 2 0.17326418285650647 1101 3 0.21331315594548883 1101 4 0.38555034322881454 1101 5 0.16903521386188913 1101 6 0.2794423179911547 1101 7 0.3979822740125698 1101 8 0.47264708336316374 1101 9 0.3895759646827912 1101 10-11 0.4377741649960285 1101 12-13 0.43450863674213736 1101 14-15 0.5025247287812604 1101 16-17 0.4864322460127255 1101 18-19 0.4954652652857092 1101 20-21 0.3511399802905899 1101 22-23 0.34394030317488244 1101 24-25 0.23645145898196773 1101 26-27 0.2628501402442751 1101 28-29 0.3358447994923992 1101 30-31 0.2671303908179965 1101 32-33 0.23483012235271872 1101 34-35 0.2136155062067573 1101 36-37 0.3290296616390762 1101 38-39 0.39622654273753 1101 40-41 0.09771007307697488 1101 42-43 0.2685424603952953 1101 44-45 0.1490321067171223 1101 46-47 0.12166734942118751 1101 48-49 0.17013081149425346 1101 50-51 0.07644850255691438 1101 52-53 0.05532721574491717 1101 54-55 0.04797642583740469 1101 56-57 0.20393775750400067 1101 58-59 0.10552984448447944 1101 60-61 0.15575490608213727 1101 62-63 0.09558023064408872 1101 64-65 -0.030953187187208187 1101 66-67 0.1477207780433787 1101 68-69 -0.08549457076826172 1101 70-71 -0.22408487684425893 1101 72-73 -0.0751805850724665 1101 74-75 -0.10223836410525422 1101 76-77 -0.19600065318633852 1101 78-79 -0.14084081913811275 1101 80-81 -0.2383372899153713 1101 82-83 -0.1397677029153641 1101 84-85 -0.006421190220805784 1101 86-87 0.027009028538039104 1101 88-89 0.6176918039333863 1101 90-91 -0.20487727762164987 1101 92-93 -0.2970376408881097 1101 94-95 -0.08599966221318223 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.15234793604811614 1102 2 0.3474025952203341 1102 3 0.32379273411494225 1102 4 0.39497471768184766 1102 5 0.4376353750909914 1102 6 0.27881810413988717 1102 7 0.4112052563746076 1102 8 0.3230412163803109 1102 9 0.5376244337904765 1102 10-11 0.5620436936361415 1102 12-13 0.5850014206418095 1102 14-15 0.5929134038481507 1102 16-17 0.5354957683981709 1102 18-19 0.5320499999634549 1102 20-21 0.4496599033774089 1102 22-23 0.4844691911018302 1102 24-25 0.4595953638003394 1102 26-27 0.3594284132108001 1102 28-29 0.33783036415499623 1102 30-31 0.37356355758974047 1102 32-33 0.5090333086664387 1102 34-35 0.4426008293393622 1102 36-37 0.47127905859830577 1102 38-39 0.3937610548626509 1102 40-41 0.42371516716388413 1102 42-43 0.39856871501809366 1102 44-45 0.47807436191707353 1102 46-47 0.44552533606488964 1102 48-49 0.4933238064974077 1102 50-51 0.5321975434946822 1102 52-53 0.44012839265464976 1102 54-55 0.37119294648822176 1102 56-57 0.34412787610462914 1102 58-59 0.41894087519015244 1102 60-61 0.43950636859323566 1102 62-63 0.5218202675322985 1102 64-65 0.5407678864606211 1102 66-67 0.612735003218333 1102 68-69 0.5365847200746217 1102 70-71 0.5420364653096215 1102 72-73 0.6405237257672098 1102 74-75 0.557121236397883 1102 76-77 0.3260522889311588 1102 78-79 0.32930434979172674 1102 80-81 0.42223231306579834 1102 82-83 0.3746456772183251 1102 84-85 0.4448987554506303 1102 86-87 0.40542926561383297 1102 88-89 0.8652474891413995 1102 90-91 -0.13774135270297005 1102 92-93 -0.0407374724444729 1102 94-95 0.08382493055785689 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.07349000031613429 1103 2 -0.016461610749136213 1103 3 -0.02555729944561591 1103 4 0.004948669761063229 1103 5 -0.006817279887975758 1103 6 0.1154028091456425 1103 7 0.03523153829562631 1103 8 0.0018635794132038086 1103 9 0.2284700983203578 1103 10-11 0.04034632983351827 1103 12-13 0.028613522148646098 1103 14-15 0.107005760045773 1103 16-17 0.13595268464455756 1103 18-19 0.04654723814490325 1103 20-21 -0.07304119827106348 1103 22-23 0.018226803806818737 1103 24-25 -0.11186861555837169 1103 26-27 -0.09223822266636006 1103 28-29 0.0032069505842144963 1103 30-31 -0.06334258984271912 1103 32-33 -0.12968110636376906 1103 34-35 -0.05107038801606478 1103 36-37 0.073838898000254 1103 38-39 -0.053390300183444594 1103 40-41 0.011984058064349767 1103 42-43 -0.03540627158847087 1103 44-45 0.014344398187105867 1103 46-47 0.03864511339519083 1103 48-49 -0.01060647374866619 1103 50-51 -0.03880459329692343 1103 52-53 -0.03505372726431233 1103 54-55 -0.010256684961412077 1103 56-57 -0.21016017818342192 1103 58-59 -0.16354882572060347 1103 60-61 -0.12619227605277672 1103 62-63 -0.06915348133617982 1103 64-65 -0.15196209574304476 1103 66-67 -0.20451543245315662 1103 68-69 -0.28609544471080994 1103 70-71 -0.2848618615887091 1103 72-73 -0.14211007002447928 1103 74-75 -0.05375484816240572 1103 76-77 -0.014764433464513615 1103 78-79 -0.11889692735692137 1103 80-81 -0.2021187981274153 1103 82-83 -0.06302097097464099 1103 84-85 -0.027059703349927133 1103 86-87 -0.22322467882958819 1103 88-89 0.5848397745231608 1103 90-91 -0.4783625617452314 1103 92-93 -0.324572058910638 1103 94-95 -0.10602469350229349 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.03205024058996031 1104 2 0.1461769701821254 1104 3 0.11158538647756089 1104 4 0.2856312370289942 1104 5 0.4187894798651257 1104 6 0.37878448350292615 1104 7 0.4399447881308305 1104 8 0.3325191795809985 1104 9 0.357873226503969 1104 10-11 0.46581226139701215 1104 12-13 0.48212074333879684 1104 14-15 0.5644351961337364 1104 16-17 0.5087413824332501 1104 18-19 0.4203591875666106 1104 20-21 0.30846086920661975 1104 22-23 0.19030237161408792 1104 24-25 0.1877373651451606 1104 26-27 0.17936392271627 1104 28-29 0.2117300524000001 1104 30-31 0.2529755020851283 1104 32-33 0.12922402937063993 1104 34-35 0.12174228052831637 1104 36-37 0.2505962540259006 1104 38-39 0.2874663546975995 1104 40-41 0.36332791855455326 1104 42-43 0.32405468604321186 1104 44-45 0.3367270913015119 1104 46-47 0.4939696175001842 1104 48-49 0.3273647014176291 1104 50-51 0.3204211512946742 1104 52-53 0.26935389543599086 1104 54-55 0.23216855624431787 1104 56-57 0.31575969956572436 1104 58-59 0.30230782916044063 1104 60-61 0.23489270027610587 1104 62-63 0.32920152555541193 1104 64-65 0.24172760282470307 1104 66-67 0.4090520152606345 1104 68-69 0.3744996292988958 1104 70-71 0.2988889624060249 1104 72-73 0.4842427794990982 1104 74-75 0.3952638490270246 1104 76-77 0.3905076346124403 1104 78-79 0.3906314480251112 1104 80-81 0.4905341652572517 1104 82-83 0.3656720235720101 1104 84-85 0.3530670420207187 1104 86-87 0.24887935425005736 1104 88-89 1.0554501298896142 1104 90-91 -0.6543453627280327 1104 92-93 -0.324572058910638 1104 94-95 -0.10602469350229349 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.2248676661342337 1105 2 -0.06716722282659049 1105 3 -0.07935412219550386 1105 4 -0.09899938725558854 1105 5 -0.006625095580268692 1105 6 -0.1177418878979708 1105 7 0.07347917221113676 1105 8 -0.2934956130579067 1105 9 -0.1610446215192738 1105 10-11 -0.18598839018351043 1105 12-13 -0.1563261763289603 1105 14-15 -0.19683171707691116 1105 16-17 -0.11571710132125901 1105 18-19 -0.06618068505233765 1105 20-21 0.10588756639777586 1105 22-23 -0.0706754394275535 1105 24-25 -0.00747483878192412 1105 26-27 -0.03344682542731192 1105 28-29 0.012495366009815712 1105 30-31 0.08917931177665395 1105 32-33 0.07471873161007636 1105 34-35 0.03326747791025042 1105 36-37 -0.03797467139454369 1105 38-39 0.03757275016588579 1105 40-41 0.07260046705621193 1105 42-43 -0.0423485601192084 1105 44-45 0.11603207207694766 1105 46-47 0.07228401977778276 1105 48-49 0.06777663532036371 1105 50-51 0.05538050088816959 1105 52-53 0.21924676620586325 1105 54-55 0.0790750162836531 1105 56-57 -0.018559075932500946 1105 58-59 0.02939978419551892 1105 60-61 0.0873104455726903 1105 62-63 0.06613909709719223 1105 64-65 0.0424892476252694 1105 66-67 -0.013053294240116031 1105 68-69 0.06322170966049612 1105 70-71 0.10663161744990646 1105 72-73 0.06233411817422052 1105 74-75 -0.11415985441579579 1105 76-77 0.15761675967480926 1105 78-79 0.1387897057809795 1105 80-81 0.03686682352968518 1105 82-83 0.05811427651439516 1105 84-85 0.07191078009709173 1105 86-87 0.10361652075529548 1105 88-89 0.699422276289777 1105 90-91 -0.7793453627280327 1105 92-93 -0.324572058910638 1105 94-95 -0.10602469350229349 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.1327079239408384 1106 2 -0.10638016676938733 1106 3 -0.011133564682896946 1106 4 0.023495171358412392 1106 5 -0.16541887069296024 1106 6 -0.05173284951543877 1106 7 -0.2181222998039729 1106 8 -0.014752420253643095 1106 9 -0.16472465928118396 1106 10-11 -0.3412951619774631 1106 12-13 -0.16357553020989712 1106 14-15 -0.2650471755664867 1106 16-17 -0.2606896103028262 1106 18-19 -0.1767559131243459 1106 20-21 -0.2435455679279741 1106 22-23 -0.10803962330707151 1106 24-25 -0.23795359333826838 1106 26-27 -0.5390572076209423 1106 28-29 -0.16328471167015834 1106 30-31 -0.06544710465910697 1106 32-33 0.057491318096793975 1106 34-35 -0.23035274102722525 1106 36-37 -0.11637652102873375 1106 38-39 -0.07759742608548947 1106 40-41 -0.18450028672508267 1106 42-43 -0.4512465009880202 1106 44-45 -0.2414575186317549 1106 46-47 -0.43262342566995926 1106 48-49 -0.2209300484790191 1106 50-51 -0.2002692166259621 1106 52-53 -0.22363907686661122 1106 54-55 -0.17057460799119895 1106 56-57 -0.29852749112701105 1106 58-59 -0.10603682329334418 1106 60-61 -0.09752476857170933 1106 62-63 -0.19711690590977327 1106 64-65 -0.14572589035429928 1106 66-67 -0.04077376871149596 1106 68-69 -0.05803742851719562 1106 70-71 -0.04714836116333743 1106 72-73 -0.055888916006715306 1106 74-75 -0.24456518304054242 1106 76-77 -0.20102812396549297 1106 78-79 -0.18593501981874283 1106 80-81 -0.24757780545083108 1106 82-83 -0.09866684136627413 1106 84-85 -0.2317717343331509 1106 86-87 -0.29313918942206385 1106 88-89 0.41631796193355086 1106 90-91 -0.3683284135754903 1106 92-93 -0.30701757949175157 1106 94-95 -0.10602469350229349 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.007390252398206343 1107 2 0.03489612242677964 1107 3 0.05297105181000816 1107 4 0.11401405155371691 1107 5 0.16494952824246667 1107 6 0.08335009527474568 1107 7 0.01723479302615516 1107 8 0.019876279244080308 1107 9 -0.03527475633359245 1107 10-11 0.1402298615352322 1107 12-13 0.0673591745306652 1107 14-15 -0.011345176486990738 1107 16-17 -0.14146522913699044 1107 18-19 0.021626213223875368 1107 20-21 0.21394147507525219 1107 22-23 0.16528797359526237 1107 24-25 0.22487633482293745 1107 26-27 0.06646977695073275 1107 28-29 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-0.2772302437408598 2216 28-29 -0.19193477033069684 2216 30-31 -0.2278768992223874 2216 32-33 -0.2576630714591346 2216 34-35 -0.26873420928813374 2216 36-37 -0.3715561221370365 2216 38-39 -0.19610794754026983 2216 40-41 -0.2486849051819391 2216 42-43 -0.3532334708589744 2216 44-45 -0.2705019758345202 2216 46-47 -0.24277918813220367 2216 48-49 -0.38530188143376876 2216 50-51 -0.2545851348506645 2216 52-53 -0.19333154585140733 2216 54-55 -0.2550957133055931 2216 56-57 -0.3502511126042549 2216 58-59 -0.310905648335158 2216 60-61 -0.39131423962834333 2216 62-63 -0.3419073504817973 2216 64-65 -0.3298338756766839 2216 66-67 -0.35682678785729394 2216 68-69 -0.5753872200850623 2216 70-71 -0.47912210120976795 2216 72-73 -0.4036123877123643 2216 74-75 -0.5021274006586722 2216 76-77 -0.44218660727843684 2216 78-79 -0.40019077978479345 2216 80-81 -0.4197876853839304 2216 82-83 -0.5875213382585116 2216 84-85 -0.6782945370589921 2216 86-87 -0.7044020136151694 2216 88-89 -0.9863841673193381 2216 90-91 -0.7793453627280327 2216 92-93 -0.324572058910638 2216 94-95 -0.10602469350229349 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7074.0 3 1165.0 4 793.0 5 778.0 6 692.0 7 782.0 8 861.0 9 1009.0 10 1185.0 11 1426.0 12 1864.0 13 2044.0 14 2407.0 15 2762.0 16 3128.0 17 3751.0 18 4720.0 19 5860.0 20 5892.0 21 8641.0 22 9813.0 23 11740.0 24 18046.0 25 30439.0 26 53713.0 27 144102.0 28 221368.0 29 8288.0 30 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.681029509331644 20.247281276141578 15.036634263132084 23.0350549513947 2 13.14469093957614 20.512922214309537 45.135532258209906 21.20685458790441 3 19.025891111608075 27.0031525259885 29.156176281750696 24.814780080652724 4 11.256120985850714 17.937036135234333 39.984128272416555 30.82271460649839 5 11.786413195415152 39.07094609828023 34.21140440268021 14.93123630362441 6 28.02521129604523 37.0665907058586 21.29510773934866 13.613090258747512 7 27.805982634782357 32.163512850837975 22.46877433870828 17.56173017567139 8 24.401267776271382 38.29109816251767 20.839703464389768 16.467930596821184 9 25.479021836328496 19.53893472178152 22.45344996682724 32.52859347506274 10-11 23.60466080610383 27.013456698421397 30.279013752370787 19.102868743103983 12-13 26.23122834820282 24.930881017993922 29.19583997028808 19.642050663515185 14-15 20.63282444399573 29.945445236103502 26.25713935442928 23.164590965471486 16-17 19.76088549709152 30.994020785275612 29.87894636735437 19.3661473502785 18-19 20.90327536033291 28.99728826272118 30.81193452885368 19.28750184809223 20-21 21.973989166209172 28.84530612355281 28.45857819341264 20.72212651682537 22-23 20.47754865089982 30.933841266636136 28.84141086708037 19.747199215383674 24-25 21.197662762858048 29.192928433380448 30.39003230738166 19.21937649637984 26-27 19.329171113739292 30.815238555398505 29.792471947385124 20.063118383477082 28-29 19.302604191550373 31.251380341414208 27.94523165847401 21.50078380856141 30-31 20.380513168142613 29.129737935212162 29.54809328756454 20.94165560908068 32-33 19.426393024264176 30.255712677280272 30.48089343937923 19.837000859076326 34-35 20.00191103931693 30.572933192950067 30.04604523033432 19.379110537398677 36-37 19.359098710589322 29.950673551215857 29.83105330717974 20.859174431015088 38-39 20.416660657109066 30.960729944904376 28.55579513658523 20.066814261401333 40-41 22.112257334064093 28.60483312660455 30.0309011451813 19.252008394150057 42-43 19.515136873683907 30.759620099806735 30.02296852914876 19.7022744973606 44-45 19.437991676154265 30.720796002679062 29.139048968623772 20.7021633525429 46-47 21.24219358467707 29.31254867741656 29.0698827415121 20.375374996394267 48-49 20.244433186519576 29.78594518191865 29.12537341955862 20.84424821200315 50-51 18.95608481777052 31.173406241351863 29.502958057156352 20.367550883721268 52-53 19.15726771857963 30.165575331006433 30.05352712954683 20.623629820867105 54-55 19.45072706637664 29.181904967092482 30.152318400174522 21.215049566356356 56-57 20.304161738829436 29.769305102835546 27.985367929154524 21.94116522918049 58-59 20.5940627974731 30.558600398073093 27.522211324891106 21.325125479562697 60-61 19.754377361756138 28.691280612686416 30.99065754175441 20.56368448380304 62-63 20.188327516081575 28.588517177719446 30.401931231430467 20.821224074768512 64-65 20.706940955141917 29.990724239482287 28.243654126811997 21.0586806785638 66-67 20.605961721521908 31.090085672253153 27.604512216228688 20.699440389996248 68-69 21.323019702688352 28.739224458484813 29.915237965603005 20.022517873223833 70-71 22.081879020394034 28.71516860414804 29.021565898404823 20.181386477053106 72-73 20.881259555196586 30.12113465255142 28.616822194017367 20.380783598234633 74-75 20.112697233680446 30.8534593417371 29.643374823319007 19.39046860126345 76-77 20.499772838722706 29.246113018144055 30.128616551763926 20.125497591369314 78-79 20.17680735354169 29.03727701892021 29.595535375442942 21.19038025209516 80-81 20.73171171431044 29.74090994317362 29.414050105287448 20.11332823722849 82-83 20.227018275764465 29.478571905046074 30.566247617948722 19.72816220124074 84-85 19.99794473130066 29.672040413072953 30.26617532523726 20.06383953038913 86-87 18.907955418215646 31.548986236762307 28.28801476924852 21.255043575773527 88-89 29.019512999750347 18.950724883198404 35.51504154927066 16.514720567780593 90-91 30.59638524750538 15.689933476814714 17.58584425748386 36.12783701819605 92-93 22.248858395757146 17.913707861095123 36.533148441028565 23.304285302119162 94-95 15.925866273612149 7.307788043398686 28.39282024751372 48.37352543547545 96-97 27.494605383590915 7.335978285028255 15.55049588469084 49.61892044668999 98-99 57.02484680818058 14.098818419215556 18.707500581741087 10.168834190862787 100-101 30.771731636269873 7.130245011668556 12.979471187352743 49.11855216470883 102-103 23.45195141538615 2.13547130226285 37.659415862622815 36.75316141972818 104-105 41.45441902809339 4.601108251672055 41.892414999390674 12.052057720843877 106-107 6.011268073628505 1.9697139981138436 6.920629956657496 85.09838797160015 108-109 18.307502106058745 11.42623226347955 22.8270990213658 47.439166609095906 110-111 40.564534722181165 2.8071554827763556 26.04001016806872 30.588299626973757 112-113 27.48766524801326 2.8505517682949795 39.47233625852134 30.189446725170427 114-115 29.658644250223098 4.137005524636062 24.15035729613003 42.05399292901081 116-117 20.214974074760285 4.304314786918964 20.92358834317445 54.5571227951463 118-119 20.885708760394508 4.39018887384774 16.131631534841745 58.592470830916014 120-121 25.817289769092337 4.360873187188468 30.404606186686795 39.4172308570324 122-123 34.471779596803394 1.7565665953738023 34.296720154978 29.474933652844808 124-125 39.27356800017394 2.133345895927364 20.2396073223012 38.35347878159749 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19.0 1 34.0 2 89.0 3 148.0 4 159.0 5 138.0 6 131.5 7 129.0 8 98.0 9 76.0 10 71.5 11 67.0 12 69.5 13 71.5 14 91.0 15 126.5 16 155.0 17 196.0 18 261.5 19 349.5 20 489.5 21 755.5 22 1079.0 23 1459.0 24 2042.0 25 2880.5 26 3907.5 27 5112.5 28 6652.5 29 8591.0 30 11168.5 31 14496.0 32 18426.0 33 22632.5 34 26421.5 35 29275.0 36 30553.5 37 30072.0 38 28528.5 39 26777.0 40 25087.0 41 23217.0 42 21509.5 43 20385.0 44 19425.0 45 18313.5 46 17130.0 47 16031.0 48 15221.5 49 14478.0 50 13559.0 51 12416.0 52 11106.0 53 9654.0 54 8200.0 55 6907.0 56 5834.5 57 4915.5 58 4097.5 59 3305.0 60 2594.0 61 2010.0 62 1447.5 63 1072.5 64 840.0 65 593.5 66 390.0 67 290.0 68 224.0 69 174.5 70 126.0 71 80.0 72 65.5 73 49.0 74 31.0 75 22.0 76 19.5 77 18.0 78 9.0 79 4.5 80 4.0 81 3.5 82 3.0 83 3.5 84 2.0 85 0.0 86 0.5 87 2.0 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 1.5 97 4.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0012620070960856146 3 0.035336198690397204 4 0.04092508725877636 5 0.04272795453889867 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.211469120489226E-4 12-13 0.0022535841001528833 14-15 0.0 16-17 9.014336400611532E-5 18-19 0.00829318948856261 20-21 0.0033353044682262667 22-23 0.0010817203680733838 24-25 0.0 26-27 0.0 28-29 0.0015324371881039606 30-31 0.0 32-33 0.0011718637320794992 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 9.014336400611532E-5 46-47 0.0 48-49 1.8028672801223065E-4 50-51 9.014336400611532E-5 52-53 0.0 54-55 0.0020732973721406525 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.014336400611532E-5 66-67 0.0 68-69 2.7043009201834596E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.014336400611532E-5 80-81 0.0 82-83 5.408601840366919E-4 84-85 0.0 86-87 0.0010817203680733838 88-89 19.119046932241034 90-91 26.28436265035913 92-93 40.324462024403616 94-95 44.591308016269075 96-97 48.491811376813686 98-99 93.02705022067096 100-101 51.75536172729108 102-103 53.08091989500101 104-105 74.85018172902184 106-107 81.55225069951251 108-109 12.577072576225229 110-111 39.0064758992702 112-113 52.13207084547265 114-115 86.76722459399429 116-117 66.42907880693456 118-119 72.03211988346267 120-121 80.4142808722993 122-123 75.84978149248565 124-125 50.24960697493294 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 554672.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.98380871086147 #Duplication Level Percentage of deduplicated Percentage of total 1 91.54503984808025 74.13665995485123 2 6.039195500689262 9.781541063906289 3 1.1648649076678326 2.8300559056970123 4 0.41326366290073646 1.3387066169401272 5 0.22009467661681198 0.8912052594707408 6 0.13003880089471773 0.6318622425988573 7 0.08680230424040578 0.49207068415869143 8 0.05162595431187587 0.3344693126806903 9 0.0494052011642926 0.36009192243696325 >10 0.2551384647316713 3.7335402274675555 >50 0.02560516198494381 1.4302448669479673 >100 0.016698985338722213 2.52203780853568 >500 0.0017812251027868149 0.9940655207272898 >1k 4.453062756967037E-4 0.5234486135808675 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1894 0.3414630628551648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1009 0.18190930856434073 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 958 0.17271468543571697 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 937 0.16892866414746013 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 730 0.13160931144892837 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 642 0.11574407938385207 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 624 0.11249891827963192 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 586 0.10564802261516716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8028672801223065E-4 0.0 20-21 0.0 0.0 0.0 2.7043009201834596E-4 0.0 22-23 0.0 0.0 0.0 3.605734560244613E-4 0.0 24-25 0.0 0.0 0.0 5.408601840366919E-4 0.0 26-27 0.0 0.0 0.0 9.014336400611533E-4 0.0 28-29 2.7043009201834596E-4 0.0 0.0 0.0017127239161161914 0.0 30-31 3.605734560244613E-4 0.0 0.0 0.0031550177402140363 0.0 32-33 4.5071682003057663E-4 0.0 0.0 0.006400178844434188 0.0 34-35 5.408601840366919E-4 0.0 0.0 0.012169354140825569 0.0 36-37 5.408601840366919E-4 0.0 0.0 0.020282256901375948 0.0 38-39 5.408601840366919E-4 0.0 0.0 0.03795035624657456 0.0 40-41 5.408601840366919E-4 0.0 0.0 0.05841289987596273 0.0 42-43 5.408601840366919E-4 0.0 0.0 0.0800473072374304 0.0 44-45 5.408601840366919E-4 0.0 0.0 0.10573816597917327 0.0 46-47 5.408601840366919E-4 0.0 0.0 0.1353051893731791 0.0 48-49 5.408601840366919E-4 0.0 0.0 0.16667508004730724 0.0 50-51 5.408601840366919E-4 0.0 0.0 0.20336342919779615 0.0 52-53 5.408601840366919E-4 0.0 0.0 0.23897005798021173 0.0 54-55 5.408601840366919E-4 0.0 0.0 0.2770105575907924 0.0 56-57 5.408601840366919E-4 0.0 0.0 0.32091037586177057 0.0 58-59 5.408601840366919E-4 0.0 0.0 0.37779083854962936 0.0 60-61 5.408601840366919E-4 0.0 0.0 0.44548850491822195 0.0 62-63 5.408601840366919E-4 0.0 0.0 0.5188652032191998 0.0 64-65 5.408601840366919E-4 0.0 0.0 0.5881854501399024 0.0 66-67 5.408601840366919E-4 0.0 0.0 0.6689539042893818 0.0 68-69 5.408601840366919E-4 0.0 0.0 0.764505870135864 0.0 70-71 5.408601840366919E-4 0.0 0.0 0.8734891972192576 0.0 72-73 5.408601840366919E-4 0.0 0.0 0.9859881154988894 0.0 74-75 5.408601840366919E-4 0.0 0.0 1.0913657080220383 0.0 76-77 5.408601840366919E-4 0.0 0.0 1.206749213949866 0.0 78-79 5.408601840366919E-4 0.0 0.0 1.2415445524562263 0.0 80-81 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 82-83 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 84-85 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 86-87 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 88-89 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 90-91 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 92-93 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 94-95 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 96-97 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 98-99 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 100-101 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 102-103 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 104-105 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 106-107 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 108-109 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 110-111 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 112-113 5.408601840366919E-4 0.0 0.0 1.2423558427322814 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCGCA 5 3.1885886E-4 5054.862 108-109 CCACGCG 25 6.330083E-10 3032.917 108-109 CGCGGCT 10 9.46148E-4 2933.0679 110-111 CCGCGCT 10 0.001275269 2527.431 108-109 CCGCGCC 70 0.0 2527.431 108-109 CCGCTCG 55 0.0 2297.6643 108-109 CCGCTCC 130 0.0 2138.5955 108-109 AAGCGTC 15 0.0028689809 1684.9539 108-109 CATCGCG 15 0.0028689809 1684.9539 108-109 CGGGTTG 35 4.5503075E-6 1676.0388 110-111 CTGCGCG 35 4.5503075E-6 1676.0388 110-111 CTTCGCT 80 2.0008883E-11 1466.5339 110-111 CTCCGCC 90 3.45608E-11 1303.5857 110-111 CAGCGCA 40 1.064002E-5 1263.7155 108-109 CGGCGCG 20 0.005099745 1263.7155 108-109 CCGTCCG 20 4.5239646E-4 1263.7155 108-109 GCGCTCG 20 0.005099745 1263.7155 108-109 GTTCGGT 25 0.0059114336 1173.2273 110-111 CGTCGCC 50 1.3261211E-5 1173.2273 110-111 CGGTATG 25 0.0059114336 1173.2273 110-111 >>END_MODULE