FastQCFastQC Report
Fri 27 May 2016
SRR939948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554672
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20530.37012865260910954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.33208815299852884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.235274180055961No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10590.19092364496495226No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9770.17614013326794933No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8730.15739031355467736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.1523422851703349No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA7150.12890501052874492No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6820.1229555485043413No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6260.11285949173565639No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6020.10853261026336286No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5760.10384515533504485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9200.055.005042
GTACCTG9600.052.732191
GTCGGAC350.00777610851.0298843
ATGGGCG505.3148286E-447.627895
ACCTGGG10800.045.2023963
GTACACG801.190021E-437.222721
CGTAGAA650.001932469436.636842
TAAGACG1001.0569453E-535.7209174
TAGGACC10650.035.2178084
CCTGGGG8050.032.5407944
GTACATG32400.031.9839671
TACATGG33150.030.8898852
ACATGGG32150.030.7396183
CGATGAT504.43972E-429.74864684-85
CATGGGT5300.028.0824814
CATGGGG13500.027.7829364
AGTACTT27650.027.1126912-13
TAGCCCT6450.026.7676124
ACGTAGA900.00948093526.469491
GAGTACT27150.026.4067212-13
Produced by FastQC (version 0.11.5)