##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 554672 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82542836126576 34.0 31.0 34.0 31.0 34.0 2 32.88807619638273 34.0 31.0 34.0 31.0 34.0 3 32.93276927625696 34.0 31.0 34.0 31.0 34.0 4 36.34515353217758 37.0 37.0 37.0 35.0 37.0 5 36.25846265901289 37.0 37.0 37.0 35.0 37.0 6 36.39860133556408 37.0 37.0 37.0 35.0 37.0 7 36.39403827847809 37.0 37.0 37.0 35.0 37.0 8 36.415346366861854 37.0 37.0 37.0 35.0 37.0 9 38.25092667378198 39.0 39.0 39.0 37.0 39.0 10-11 38.23833544869761 39.0 39.0 39.0 37.0 39.0 12-13 38.228017639253466 39.0 39.0 39.0 37.0 39.0 14-15 39.72053123287276 41.0 40.0 41.0 37.0 41.0 16-17 39.748381025182454 41.0 40.0 41.0 37.0 41.0 18-19 39.81607238151556 41.0 40.0 41.0 37.5 41.0 20-21 39.84613609484524 41.0 40.0 41.0 38.0 41.0 22-23 39.78938453716791 41.0 40.0 41.0 37.0 41.0 24-25 39.759352374015634 41.0 40.0 41.0 37.0 41.0 26-27 39.65889120056538 41.0 40.0 41.0 37.0 41.0 28-29 39.579046715896965 41.0 40.0 41.0 37.0 41.0 30-31 39.42905717252719 41.0 39.0 41.0 36.0 41.0 32-33 39.2503218118095 41.0 39.0 41.0 35.0 41.0 34-35 39.138206904260535 41.0 39.0 41.0 35.0 41.0 36-37 39.10817744540918 41.0 39.0 41.0 35.0 41.0 38-39 39.14574379092509 41.0 39.0 41.0 35.0 41.0 40-41 39.04815188075115 41.0 39.0 41.0 35.0 41.0 42-43 38.94257957856175 41.0 39.0 41.0 35.0 41.0 44-45 38.77615149133182 41.0 39.0 41.0 35.0 41.0 46-47 38.502450998067324 41.0 39.0 41.0 35.0 41.0 48-49 38.228349366833015 41.0 39.0 41.0 35.0 41.0 50-51 38.05461425851674 41.0 39.0 41.0 34.0 41.0 52-53 37.95986547004355 41.0 39.0 41.0 34.0 41.0 54-55 37.85735353506216 41.0 39.0 41.0 34.0 41.0 56-57 37.710588419822884 41.0 38.0 41.0 34.0 41.0 58-59 37.56426590850088 40.0 38.0 41.0 33.0 41.0 60-61 37.374818811838345 40.0 37.5 41.0 33.0 41.0 62-63 37.18526534600629 40.0 37.0 41.0 33.0 41.0 64-65 36.95403499725964 40.0 37.0 41.0 32.5 41.0 66-67 36.676701726425705 39.0 36.0 41.0 32.0 41.0 68-69 36.34138644099576 39.0 35.5 41.0 32.0 41.0 70-71 35.968796874549284 38.5 35.0 40.5 31.0 41.0 72-73 35.62528665589754 37.0 35.0 40.0 31.0 41.0 74-75 35.27435493408717 37.0 35.0 39.0 31.0 41.0 76-77 34.88736766954164 36.5 35.0 39.0 31.0 41.0 78-79 34.508783569388754 36.0 35.0 39.0 30.5 40.0 80-81 34.21442582282863 36.0 35.0 37.0 30.5 39.0 82-83 33.95805629272795 35.0 35.0 37.0 30.5 39.0 84-85 33.662238223670926 35.0 35.0 37.0 30.0 39.0 86-87 33.43372389448179 35.0 35.0 36.0 30.0 37.0 88-89 33.198236074653124 35.0 35.0 36.0 30.0 37.0 90-91 32.99366923154585 35.0 34.0 36.0 30.0 37.0 92-93 32.84990228459341 35.0 34.0 35.0 30.0 36.0 94-95 32.741040651051435 35.0 34.0 35.0 29.5 36.0 96-97 32.60985771771425 35.0 34.0 35.0 29.0 36.0 98-99 32.49092526754551 35.0 34.0 35.0 29.0 36.0 100-101 32.4826401909597 35.0 34.0 35.0 29.0 36.0 102-103 31.638840612109497 34.5 32.5 35.0 26.5 35.0 104-105 32.30403463668618 35.0 34.0 35.0 29.0 35.0 106-107 32.36857277814636 35.0 34.0 35.0 29.0 35.0 108-109 32.377761091239506 35.0 34.0 35.0 29.0 35.0 110-111 32.36075824992067 35.0 34.0 35.0 29.0 35.0 112-113 32.277346792338534 35.0 34.0 35.0 29.0 35.0 114-115 32.20383307612427 35.0 34.0 35.0 29.0 35.0 116-117 32.14506591282776 35.0 34.0 35.0 29.0 35.0 118-119 32.06782566994548 35.0 34.0 35.0 29.0 35.0 120-121 31.97260813597946 35.0 34.0 35.0 29.0 35.0 122-123 31.798977593965443 35.0 34.0 35.0 27.0 35.0 124-125 29.78203605013413 33.5 29.5 34.5 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.00853400084382372 1101 2 0.09283559290108911 1101 3 0.08431853109892273 1101 4 0.011566784700264066 1101 5 -0.07555768330879431 1101 6 0.0175259242137642 1101 7 0.08135629785152787 1101 8 0.1632178686585135 1101 9 0.14461874757066084 1101 10-11 0.16407498017930777 1101 12-13 0.07645050961946254 1101 14-15 0.1957530641598737 1101 16-17 0.21730302483613428 1101 18-19 0.2596631919395307 1101 20-21 0.16388413410782476 1101 22-23 0.1974237266821035 1101 24-25 0.16427365573799335 1101 26-27 0.11111736820534901 1101 28-29 0.21820162736756998 1101 30-31 0.20461531253852883 1101 32-33 0.35656620812362405 1101 34-35 0.24573076026307206 1101 36-37 0.2844306504164962 1101 38-39 0.19495186329002934 1101 40-41 0.27843312532395714 1101 42-43 0.24477288589125834 1101 44-45 0.3130156171887961 1101 46-47 0.2793959650194253 1101 48-49 0.39877029627297844 1101 50-51 0.3726852383310515 1101 52-53 0.3582787255739319 1101 54-55 0.23218540102118368 1101 56-57 0.3367270214144469 1101 58-59 0.3482262554301627 1101 60-61 0.3124603790005054 1101 62-63 0.30287380709060585 1101 64-65 0.3701841074144525 1101 66-67 0.37000571106126046 1101 68-69 0.4320511230292752 1101 70-71 0.46225906367370584 1101 72-73 0.3951769866926895 1101 74-75 0.2977999747647999 1101 76-77 0.43150153968886684 1101 78-79 0.33093388216305186 1101 80-81 0.3609660127088077 1101 82-83 0.415441825326738 1101 84-85 0.3440371877356654 1101 86-87 0.21661169696467653 1101 88-89 0.2566838321571723 1101 90-91 0.29144267600631224 1101 92-93 0.2813348600992782 1101 94-95 0.2700995904192709 1101 96-97 0.4482328681313916 1101 98-99 0.3888358648777057 1101 100-101 0.22358957869629847 1101 102-103 0.2777719224310964 1101 104-105 0.2881625718155689 1101 106-107 0.3913642076081416 1101 108-109 0.31927301777675154 1101 110-111 0.2645728796552902 1101 112-113 0.28298222492831826 1101 114-115 0.3937260254928816 1101 116-117 0.3965641718558004 1101 118-119 0.26205434899429747 1101 120-121 0.33958422539999233 1101 122-123 0.2956169011165706 1101 124-125 0.36004398104783064 1102 1 0.12861988514155343 1102 2 0.1805313654990428 1102 3 0.13070483478053063 1102 4 0.06463907744714703 1102 5 -0.022703394896900875 1102 6 0.11829725178174044 1102 7 0.1070055273081465 1102 8 -0.0014804854871712791 1102 9 0.10500783041544537 1102 10-11 0.15079475207462423 1102 12-13 0.04675722132990501 1102 14-15 0.16430711511956275 1102 16-17 0.2523429086508102 1102 18-19 0.18519432264488955 1102 20-21 0.23533466280754567 1102 22-23 0.25692793149953275 1102 24-25 0.28511549751389254 1102 26-27 0.23491403132749156 1102 28-29 0.3438944576911922 1102 30-31 0.2423888766114075 1102 32-33 0.2852795747692767 1102 34-35 0.45609710164845296 1102 36-37 0.46975315567839715 1102 38-39 0.3920983590663951 1102 40-41 0.3583968017239627 1102 42-43 0.49563314040739925 1102 44-45 0.6113561180027816 1102 46-47 0.7784690545015849 1102 48-49 0.7804645193149398 1102 50-51 0.7647864237100279 1102 52-53 0.7790764959698819 1102 54-55 0.7379068385323535 1102 56-57 0.8104418152827151 1102 58-59 0.871897762106741 1102 60-61 0.7857407325753059 1102 62-63 0.6236373868786274 1102 64-65 0.7909834461869139 1102 66-67 0.8067507338183972 1102 68-69 0.74754417355296 1102 70-71 0.9164295336534067 1102 72-73 0.9227584560105413 1102 74-75 1.0240500492626836 1102 76-77 0.9350241133319841 1102 78-79 0.8639309555724779 1102 80-81 0.8140550870843555 1102 82-83 0.7824513390986425 1102 84-85 0.7776368314005282 1102 86-87 0.8026559690968256 1102 88-89 0.8192926382776093 1102 90-91 0.8174910572849114 1102 92-93 0.7564376671795614 1102 94-95 0.7832716005447153 1102 96-97 0.6958003161632362 1102 98-99 0.7546275342890283 1102 100-101 0.7363836100826475 1102 102-103 0.7870535746216483 1102 104-105 0.857182901111905 1102 106-107 0.8077179831609129 1102 108-109 0.8013347114351745 1102 110-111 0.8430528718761465 1102 112-113 0.8651541754554444 1102 114-115 0.9646676160148786 1102 116-117 1.0494887235353758 1102 118-119 0.9097005295269796 1102 120-121 1.0304383946152633 1102 122-123 1.1384742008113697 1102 124-125 0.9648460362484101 1103 1 0.017836169094678667 1103 2 0.005957954083527284 1103 3 0.04593517106349765 1103 4 -0.008427495619429237 1103 5 -0.0198646025844198 1103 6 0.04795247091064425 1103 7 -0.01940694120958142 1103 8 0.1133889978033622 1103 9 0.1288235121258623 1103 10-11 0.11673386275057851 1103 12-13 0.013709306703042046 1103 14-15 0.03163514034233117 1103 16-17 0.0226747579300266 1103 18-19 -0.03469508145503397 1103 20-21 -0.014571429203812158 1103 22-23 0.07854739785584286 1103 24-25 0.13358956967217495 1103 26-27 0.19238756774839771 1103 28-29 0.17392253771926391 1103 30-31 0.1896895607542035 1103 32-33 0.19415957575454001 1103 34-35 0.2628252241660505 1103 36-37 0.22078152512781912 1103 38-39 0.1693455310503964 1103 40-41 0.21004835074393924 1103 42-43 0.28126275498437536 1103 44-45 0.33189056222143876 1103 46-47 0.4128052019956243 1103 48-49 0.44314394515226496 1103 50-51 0.4863815663577995 1103 52-53 0.6415105294565535 1103 54-55 0.5012448518703678 1103 56-57 0.4671126693219918 1103 58-59 0.55787252702887 1103 60-61 0.5923549339263516 1103 62-63 0.5147410674559936 1103 64-65 0.37882282349997354 1103 66-67 0.42364462702808936 1103 68-69 0.3436582301933342 1103 70-71 0.45252868817362213 1103 72-73 0.43491877080243313 1103 74-75 0.43451570972834475 1103 76-77 0.44419784618992963 1103 78-79 0.4691710213388589 1103 80-81 0.44453330966783966 1103 82-83 0.4097859532753674 1103 84-85 0.35732006897849544 1103 86-87 0.4052481374784662 1103 88-89 0.36733812053362414 1103 90-91 0.4618368726833637 1103 92-93 0.4723906331325267 1103 94-95 0.5125210140046548 1103 96-97 0.5718417711520587 1103 98-99 0.5841352716915011 1103 100-101 0.436556954992831 1103 102-103 0.4465662999238482 1103 104-105 0.4519545353572241 1103 106-107 0.599896525339453 1103 108-109 0.5376041319151241 1103 110-111 0.5570722569481603 1103 112-113 0.5893725838843835 1103 114-115 0.6822139388243542 1103 116-117 0.6213714401549382 1103 118-119 0.6019501954608586 1103 120-121 0.6730923267428786 1103 122-123 0.5173590665383365 1103 124-125 0.4184216214029455 1104 1 0.0617056483300118 1104 2 0.1299017426614597 1104 3 0.11317014439603668 1104 4 0.05012107512426667 1104 5 0.14306162741497985 1104 6 0.13737237862324037 1104 7 0.06205167927342359 1104 8 0.07177276960176471 1104 9 0.03339893011591499 1104 10-11 0.08242871503079385 1104 12-13 0.15279212566390044 1104 14-15 0.09144966662027088 1104 16-17 0.023158667560274182 1104 18-19 0.016308693938981378 1104 20-21 0.09636223172985581 1104 22-23 0.03014145310842764 1104 24-25 0.0416302600299403 1104 26-27 0.07625824552151528 1104 28-29 0.08483522424171497 1104 30-31 0.1960672799367984 1104 32-33 0.15915328343117352 1104 34-35 0.11241472497505356 1104 36-37 7.299880031155226E-4 1104 38-39 0.0750970142409102 1104 40-41 0.09607789470030781 1104 42-43 0.1552410015235992 1104 44-45 0.16046669933415814 1104 46-47 0.29285104606585577 1104 48-49 0.38421933317886925 1104 50-51 0.3771588181106651 1104 52-53 0.524462827652755 1104 54-55 0.5451368036891466 1104 56-57 0.5641507828235177 1104 58-59 0.40531514703247495 1104 60-61 0.41705529948037423 1104 62-63 0.4774395752764491 1104 64-65 0.36804347430599194 1104 66-67 0.3834531606171012 1104 68-69 0.4600602197660564 1104 70-71 0.42934597108845907 1104 72-73 0.28699373934081507 1104 74-75 0.36731369618487264 1104 76-77 0.37475606516277793 1104 78-79 0.3163424721688415 1104 80-81 0.3068602707752959 1104 82-83 0.3589230060709596 1104 84-85 0.34737520171655945 1104 86-87 0.3813313160006686 1104 88-89 0.3765638651252132 1104 90-91 0.4273953912906592 1104 92-93 0.4852704197927977 1104 94-95 0.5259086810508578 1104 96-97 0.4814825094513324 1104 98-99 0.5413519673228322 1104 100-101 0.5889525323884115 1104 102-103 0.5643061572978461 1104 104-105 0.5349397933424811 1104 106-107 0.5026366823479052 1104 108-109 0.4870902588402828 1104 110-111 0.5137585996101137 1104 112-113 0.5091559481799379 1104 114-115 0.4994099596789141 1104 116-117 0.571918273018845 1104 118-119 0.5501583814739206 1104 120-121 0.47088402087604564 1104 122-123 0.6528088178417413 1104 124-125 0.7682478334242759 1105 1 0.06327149611845329 1105 2 0.14650677333271034 1105 3 0.005472982586873343 1105 4 0.06059919434589034 1105 5 -0.03667333780470727 1105 6 0.07132060438708265 1105 7 0.11266950675133813 1105 8 0.025717473308731087 1105 9 0.1199549454955644 1105 10-11 0.18046957089061522 1105 12-13 0.10021442028396166 1105 14-15 0.12536047052975618 1105 16-17 0.14235901389587013 1105 18-19 0.093161442610878 1105 20-21 0.12409989719314751 1105 22-23 0.15289981065591007 1105 24-25 0.11274865984209015 1105 26-27 0.14689162875065165 1105 28-29 0.1409547980728263 1105 30-31 -0.02352636275053044 1105 32-33 0.04775209180192519 1105 34-35 0.05329406517640223 1105 36-37 0.059526325063956165 1105 38-39 -0.17548485890634424 1105 40-41 -0.16434959495364154 1105 42-43 -0.25699300980568296 1105 44-45 -0.47991221528531725 1105 46-47 -0.5080052540133906 1105 48-49 -0.6486950221379715 1105 50-51 -0.6480077458293465 1105 52-53 -0.5701110034646177 1105 54-55 -0.7757600000442295 1105 56-57 -0.7448385336039394 1105 58-59 -0.7895301759694959 1105 60-61 -0.6300030492619584 1105 62-63 -0.7194111188622543 1105 64-65 -0.6858109766933893 1105 66-67 -0.6236415827129989 1105 68-69 -0.5666619192965001 1105 70-71 -0.625222430191215 1105 72-73 -0.5697091751612575 1105 74-75 -0.6589050822483955 1105 76-77 -0.635228232333624 1105 78-79 -0.5897800367096693 1105 80-81 -0.5518891055271382 1105 82-83 -0.43107435238638203 1105 84-85 -0.6636649790823625 1105 86-87 -0.5634442996255231 1105 88-89 -0.5150110813101705 1105 90-91 -0.5138178105525526 1105 92-93 -0.7081059119849655 1105 94-95 -0.6424881960587072 1105 96-97 -0.5672693616413866 1105 98-99 -0.610389249547822 1105 100-101 -0.5468176240496199 1105 102-103 -0.6271814714773534 1105 104-105 -0.5301324088235866 1105 106-107 -0.6140630036020198 1105 108-109 -0.5747431185187679 1105 110-111 -0.5513899137883769 1105 112-113 -0.6512451260474066 1105 114-115 -0.5726571942056253 1105 116-117 -0.6149657339078693 1105 118-119 -0.5698263562795916 1105 120-121 -0.8110199443729975 1105 122-123 -0.7936779304192392 1105 124-125 -0.7328865623962173 1106 1 -0.05607915058335067 1106 2 -0.015509584333166515 1106 3 -0.10265398600023445 1106 4 -0.08709619293219362 1106 5 -0.047468697985131314 1106 6 0.06689756612692577 1106 7 0.045474581299053796 1106 8 -0.09030423978818192 1106 9 -0.029162340775243933 1106 10-11 0.0038277583190478026 1106 12-13 -0.09150156121969388 1106 14-15 -0.26062173242301867 1106 16-17 -0.12224823021330877 1106 18-19 -0.029070152101290603 1106 20-21 -0.003153506768938996 1106 22-23 0.06052590949536807 1106 24-25 0.02788343430501783 1106 26-27 0.06835356235846035 1106 28-29 0.11326491773961322 1106 30-31 0.08310294993029999 1106 32-33 0.20637468542727788 1106 34-35 0.1203637003485909 1106 36-37 0.05506176618771974 1106 38-39 0.013694722857223951 1106 40-41 0.03884792954351468 1106 42-43 0.10368929774990221 1106 44-45 -0.03691710743368759 1106 46-47 -0.03783016813127915 1106 48-49 0.05435900128257032 1106 50-51 0.1357456013150511 1106 52-53 0.2819969326548204 1106 54-55 0.3281005431328339 1106 56-57 0.2651060181627969 1106 58-59 0.2785818451958235 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-0.1447904903761028 2216 68-69 0.02993701131747173 2216 70-71 0.028553916776168364 2216 72-73 0.10269024424400897 2216 74-75 -0.009037825062300442 2216 76-77 -0.02487305226241432 2216 78-79 -0.00846265732575091 2216 80-81 -0.22370296784678345 2216 82-83 -0.16794397909374226 2216 84-85 -0.17466402383033142 2216 86-87 -0.12558344386857812 2216 88-89 -0.1035970601953764 2216 90-91 -0.15444393483456054 2216 92-93 -0.09812787037567006 2216 94-95 -0.0927108814334261 2216 96-97 -0.13378653756903702 2216 98-99 -0.08250395422607681 2216 100-101 -0.13759385936416635 2216 102-103 -0.17692466995887557 2216 104-105 -0.16222515511113045 2216 106-107 -0.25224765257791404 2216 108-109 -0.3396698618537073 2216 110-111 -0.319677407430369 2216 112-113 -0.28662604829981575 2216 114-115 -0.28158073682599394 2216 116-117 -0.4645476560734352 2216 118-119 -0.38485544804581906 2216 120-121 -0.626991262000498 2216 122-123 -0.5612092862568225 2216 124-125 -0.5178723576745412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52.0 3 0.0 4 10.0 5 27.0 6 59.0 7 147.0 8 256.0 9 491.0 10 1186.0 11 1665.0 12 1862.0 13 2896.0 14 5012.0 15 5605.0 16 1960.0 17 1251.0 18 987.0 19 827.0 20 821.0 21 885.0 22 906.0 23 1018.0 24 1312.0 25 1491.0 26 1970.0 27 2491.0 28 3227.0 29 4083.0 30 5029.0 31 6723.0 32 8576.0 33 11642.0 34 16831.0 35 27751.0 36 60217.0 37 192478.0 38 180656.0 39 2272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12776469485126 20.101806405545076 15.423147523720568 22.347281375883096 2 13.440375573311794 20.73081028067038 44.75473793521216 21.074076210805664 3 19.22938240978452 27.431166527244933 29.34346785127066 23.995983211699887 4 11.266838780396341 17.89255632157383 39.65262353246604 31.187981365563793 5 11.293955835688836 38.59233026847309 34.35296793268137 15.760745963156698 6 27.635251103354776 37.43888279920385 20.77461995557735 14.151246141864021 7 26.885258314823897 32.741692387573195 22.771475755040818 17.60157354256209 8 24.684678512706608 37.131674214671015 20.74126691089509 17.44238036172729 9 25.802816799838464 18.613883520350765 22.900921625753597 32.68237805405717 10-11 23.58447875501197 26.711281622292095 30.134926587244355 19.569313035451582 12-13 26.759508322035362 24.674402169209912 29.540881818444053 19.02520769031067 14-15 20.926511523927655 29.046445466870512 27.651747338967898 22.375295670233943 16-17 19.12779083854963 30.34541134219863 30.984888366458012 19.541909452793725 18-19 20.028413188334728 27.620287304929757 33.46329001644215 18.88800949029336 20-21 21.033273535324394 27.349565938573324 31.625904858062366 19.991255668039916 22-23 19.605350929381434 29.400995186327005 31.973786215227072 19.01986766906449 24-25 20.403229295872155 27.64426543975539 33.50854198517322 18.443963279199238 26-27 18.577014884472266 29.17760406149941 32.982104739377505 19.26327631465082 28-29 18.42512331612196 29.693043816886377 31.268299102893245 20.61353376409842 30-31 19.588243141892868 27.601627628580495 32.70842948625494 20.101699743271702 32-33 18.683023480543458 28.756093691406814 33.50574754088903 19.055135287160702 34-35 19.281214844088037 29.00047235122739 32.99211065278219 18.726202151902385 36-37 18.754056451380276 28.379745146681284 32.72285242449592 20.143345977442525 38-39 19.768349583177088 29.43279992500072 31.543236363111895 19.255614128710302 40-41 21.47305073989673 27.178584821299793 32.67417500793262 18.674189430870857 42-43 19.0344924568033 29.429284333804485 32.38896140421726 19.14726180517495 44-45 19.058110018172904 29.39638200594225 31.409463610926817 20.136044364958032 46-47 21.00908284535726 28.139783511697004 30.882485504947066 19.968648137998674 48-49 20.302358871549313 28.856062682089593 30.42482764588802 20.41675080047307 50-51 19.028993711598925 30.294750771627193 30.65829895866386 20.01795655811002 52-53 19.260391727002624 29.409002076903107 31.08666022442092 20.243945971673348 54-55 19.530911962384977 28.457358583090546 31.124340150575474 20.887389303949 56-57 20.229613176796377 29.043470735858307 29.121354602359595 21.60556148498572 58-59 20.57278896356766 29.607768194536593 28.717061614792165 21.102381227103585 60-61 19.750501197103873 27.861601090374133 32.15161392678916 20.236283785732827 62-63 20.095479851155275 27.868361842674588 31.545309660484033 20.4908486456861 64-65 20.608305448986066 29.180037932327572 29.519968557994638 20.691688060691725 66-67 20.38898664435919 30.245622638243862 28.916999596157726 20.44839112123922 68-69 21.20343193815444 27.901624743992848 31.147236565033026 19.747706752819685 70-71 22.008051605273028 27.869894279862695 30.213711887385696 19.90834222747858 72-73 20.78408500879799 29.184184527071857 29.999891827963193 20.03183863616696 74-75 20.01353953327372 29.917951510081636 30.876355756194652 19.192153200449997 76-77 20.36293521216142 28.24984855914847 31.37647113970058 20.01074508898953 78-79 20.00046874549283 28.17584085729945 30.89871131046817 20.92497908673955 80-81 20.542140219805578 28.93115210430669 30.553281939596733 19.973425736291 82-83 20.135683791502004 28.716610897972135 31.674304814376786 19.473400496149075 84-85 19.869490437591946 28.92529278564629 31.27019211353737 19.935024663224393 86-87 18.809765050336054 30.672541610176822 29.444698849049527 21.07299449043759 88-89 19.871383448236074 28.867060172498345 30.338470303170162 20.923086076095423 90-91 20.126671167886172 28.87403105841459 31.209406767514412 19.78989100618482 92-93 19.48331626618975 30.03757175411775 31.07836703493236 19.400744944760145 94-95 19.464939743070836 30.19553190971512 30.33201031610887 20.007518031105178 96-97 20.61713949865867 29.505996336573688 30.06731906423977 19.809545100527878 98-99 20.58453932957006 28.661574048198986 31.077112243738945 19.67677437849201 100-101 19.69326016095999 28.80720497879828 31.26045663022471 20.239078230017018 102-103 19.488126691888937 29.63779266354588 30.440884961017257 20.433195683547922 104-105 20.20663220693149 28.950865129260066 30.236794992220418 20.60570767158803 106-107 19.758544342595865 28.582349504900513 30.4204018491407 21.238704303362926 108-109 19.292427402910914 29.551031151799894 29.926659226820945 21.229882218468248 110-111 18.85779553510578 29.92032641110057 30.288494477479006 20.93338357631464 112-113 19.874978476428648 28.362162147543067 29.87038978624276 21.89246958978552 114-115 19.654167710900722 29.115108549256895 28.649305035942685 22.581418703899697 116-117 20.16507424825501 29.093074219408482 27.841315257378625 22.90053627495788 118-119 19.836966564894716 28.138907443414706 29.82224614028303 22.201879851407543 120-121 19.36953219721725 28.120089963807143 30.462935001600055 22.047442837375545 122-123 20.23089654277184 28.80482598474031 29.254557714219782 21.70971975826807 124-125 20.8522487569006 29.06770249268924 28.493298621502827 21.586750128907333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 71.0 1 51.5 2 163.0 3 220.0 4 88.0 5 38.0 6 79.0 7 236.5 8 403.0 9 413.5 10 330.0 11 251.0 12 254.5 13 315.5 14 399.0 15 480.5 16 561.0 17 690.5 18 819.0 19 930.0 20 1117.0 21 1386.0 22 1648.5 23 1995.5 24 2427.0 25 2950.5 26 3602.5 27 4506.0 28 5632.5 29 6888.0 30 8477.0 31 10727.0 32 14092.0 33 18637.5 34 24283.0 35 30192.5 36 34377.5 37 35268.5 38 32512.5 39 27552.5 40 23158.5 41 20371.5 42 18201.5 43 16749.5 44 15998.0 45 15487.5 46 15153.0 47 16072.5 48 18462.0 49 17497.0 50 14626.0 51 12941.0 52 11335.5 53 10371.0 54 9485.0 55 8337.5 56 7225.5 57 6208.0 58 5213.5 59 4209.0 60 3242.0 61 2524.0 62 1933.5 63 1371.0 64 937.0 65 671.5 66 470.5 67 383.0 68 300.0 69 183.5 70 134.5 71 113.0 72 86.5 73 70.0 74 46.0 75 31.5 76 18.5 77 9.0 78 5.5 79 2.0 80 0.0 81 0.0 82 0.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01622580552110076 2 0.0 3 0.0 4 0.0 5 0.11718637320794992 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004867741656330227 22-23 3.605734560244613E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00135215046009173 92-93 0.0 94-95 9.915770040672687E-4 96-97 0.0 98-99 0.0023437274641589984 100-101 0.0 102-103 6.310035480428073E-4 104-105 0.00270430092018346 106-107 0.006490322208440303 108-109 1.8028672801223065E-4 110-111 0.006129748752415842 112-113 0.008563619580580956 114-115 0.007031182392476995 116-117 0.0022535841001528833 118-119 9.014336400611532E-5 120-121 8.112902760550379E-4 122-123 0.0014422938240978452 124-125 0.0018028672801223067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 554672.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.34494954923056 #Duplication Level Percentage of deduplicated Percentage of total 1 90.86797443319465 71.19046872609407 2 6.512399853990547 10.204272760106118 3 1.1937571177858726 2.805745235009072 4 0.4489059106867306 1.406780437004132 5 0.24207373571054036 0.9482627305718025 6 0.152793604610206 0.7182364346779002 7 0.10614023946898116 0.5820886194438405 8 0.0746192974431894 0.4676836074868562 9 0.05036951039839256 0.35515770758833576 >10 0.3000941247956766 4.4311264325211805 >50 0.02670215041558065 1.484222350347928 >100 0.02117754264736373 3.119208599329895 >500 0.0020717161285393445 1.1579809445108173 >1k 9.207627237952643E-4 1.1287654153080837 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2053 0.37012865260910954 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1842 0.33208815299852884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1305 0.235274180055961 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1059 0.19092364496495226 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 977 0.17614013326794933 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 873 0.15739031355467736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.1523422851703349 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 715 0.12890501052874492 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 682 0.1229555485043413 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 626 0.11285949173565639 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 602 0.10853261026336286 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 576 0.10384515533504485 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8028672801223065E-4 0.0 20-21 0.0 0.0 0.0 2.7043009201834596E-4 0.0 22-23 0.0 0.0 0.0 3.605734560244613E-4 0.0 24-25 0.0 0.0 0.0 5.408601840366919E-4 0.0 26-27 0.0 0.0 0.0 9.014336400611533E-4 0.0 28-29 0.0 0.0 0.0 0.0017127239161161914 0.0 30-31 0.0 0.0 0.0 0.0031550177402140363 0.0 32-33 0.0 0.0 0.0 0.006760752300458649 0.0 34-35 0.0 0.0 0.0 0.012710214324862261 0.0 36-37 0.0 0.0 0.0 0.02082311708541264 0.0 38-39 0.0 0.0 0.0 0.03849121643061125 0.0 40-41 0.0 0.0 0.0 0.05931433351602389 0.0 42-43 0.0 0.0 0.0 0.08157974442553437 0.0 44-45 0.0 0.0 0.0 0.1075410332592956 0.0 46-47 0.0 0.0 0.0 0.1370179132892953 0.0 48-49 0.0 0.0 0.0 0.16856809069143563 0.0 50-51 0.0 0.0 0.0 0.20489586638590013 0.0 52-53 0.0 0.0 0.0 0.24032220844030347 0.0 54-55 0.0 0.0 0.0 0.27845285141489023 0.0 56-57 0.0 0.0 0.0 0.3226230997778867 0.0 58-59 0.0 0.0 0.0 0.3804951394698128 0.0 60-61 0.0 0.0 0.0 0.44675051201430754 0.0 62-63 0.0 0.0 0.0 0.5202173536792916 0.0 64-65 0.0 0.0 0.0 0.5894474572359881 0.0 66-67 0.0 0.0 0.0 0.6703060547494736 0.0 68-69 0.0 0.0 0.0 0.7661284506879742 0.0 70-71 0.0 9.014336400611532E-5 0.0 0.8752019211353737 0.0 72-73 0.0 1.8028672801223065E-4 0.0 0.987971269507024 0.0 74-75 0.0 1.8028672801223065E-4 0.0 1.093439005394179 0.0 76-77 0.0 1.8028672801223065E-4 0.0 1.2092732281420373 0.0 78-79 0.0 1.8028672801223065E-4 0.0 1.3590013557561949 0.0 80-81 0.0 1.8028672801223065E-4 0.0 1.5159409524908414 0.0 82-83 0.0 1.8028672801223065E-4 0.0 1.6810835953500447 0.0 84-85 0.0 1.8028672801223065E-4 0.0 1.8451445178411747 0.0 86-87 0.0 1.8028672801223065E-4 0.0 2.001633397755791 0.0 88-89 0.0 1.8028672801223065E-4 0.0 2.183362419592119 0.0 90-91 0.0 1.8028672801223065E-4 0.0 2.3810468168575305 0.0 92-93 0.0 1.8028672801223065E-4 0.0 2.5928837222719014 0.0 94-95 0.0 1.8028672801223065E-4 0.0 2.8103996596186573 0.0 96-97 0.0 1.8028672801223065E-4 0.0 3.0387328006461476 0.0 98-99 0.0 1.8028672801223065E-4 0.0 3.2903229295872154 0.0 100-101 0.0 1.8028672801223065E-4 0.0 3.5525499754810053 0.0 102-103 0.0 1.8028672801223065E-4 0.0 3.8333465543600544 0.0 104-105 0.0 1.8028672801223065E-4 0.0 4.1116191190469324 0.0 106-107 0.0 1.8028672801223065E-4 0.0 4.385835232353535 0.0 108-109 0.0 1.8028672801223065E-4 0.0 4.6887169354140825 0.0 110-111 0.0 1.8028672801223065E-4 0.0 5.020895231776617 0.0 112-113 0.0 1.8028672801223065E-4 0.0 5.396252199498082 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 920 0.0 55.00504 2 GTACCTG 960 0.0 52.73219 1 GTCGGAC 35 0.007776108 51.029884 3 ATGGGCG 50 5.3148286E-4 47.62789 5 ACCTGGG 1080 0.0 45.202396 3 GTACACG 80 1.190021E-4 37.22272 1 CGTAGAA 65 0.0019324694 36.63684 2 TAAGACG 100 1.0569453E-5 35.720917 4 TAGGACC 1065 0.0 35.217808 4 CCTGGGG 805 0.0 32.540794 4 GTACATG 3240 0.0 31.983967 1 TACATGG 3315 0.0 30.889885 2 ACATGGG 3215 0.0 30.739618 3 CGATGAT 50 4.43972E-4 29.748646 84-85 CATGGGT 530 0.0 28.082481 4 CATGGGG 1350 0.0 27.782936 4 AGTACTT 2765 0.0 27.11269 12-13 TAGCCCT 645 0.0 26.767612 4 ACGTAGA 90 0.009480935 26.46949 1 GAGTACT 2715 0.0 26.40672 12-13 >>END_MODULE