FastQCFastQC Report
Fri 27 May 2016
SRR939947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531141
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23550.15380686690513806No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19310.12611509978506227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC3100.02167.2834108-109
CTCCGCC1800.01838.0525110-111
CGTCGCG406.37252E-61503.8612110-111
CCGCGCG1100.01357.2886108-109
CCGTTCG602.277011E-81244.1813108-109
CCGCGCC4150.01139.2502108-109
CTCGTCG300.0081259141002.5742110-111
CCGCACC1550.0963.2371108-109
CCGCTCG1650.0904.8591108-109
CGCCGTC703.4127363E-5859.3493110-111
CCGCTAC603.7994825E-5829.45416108-109
CCGCGCT603.577768E-5829.45416108-109
CGTCGCT754.1969968E-5802.05927110-111
CGTCGCC1651.8189894E-12754.04926108-109
GTTCGCT805.092969E-5751.9306110-111
CAGCGCG1001.7547427E-7746.5087108-109
CTCCGTC1707.6579454E-10707.69934110-111
TTGTTCG3250.0689.08496108-109
CGACGCC1102.568304E-7678.6443108-109
CGTCTCT2405.456968E-12626.6089110-111
Produced by FastQC (version 0.11.5)