##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939947_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531141 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.879346186928572 34.0 31.0 34.0 30.0 34.0 2 32.001543946638485 34.0 31.0 34.0 30.0 34.0 3 32.093071114939775 34.0 31.0 34.0 30.0 34.0 4 35.33842735580851 37.0 35.0 37.0 33.0 37.0 5 35.39690074264878 37.0 35.0 37.0 33.0 37.0 6 35.39820238632497 37.0 37.0 37.0 33.0 37.0 7 35.402382275701584 37.0 37.0 37.0 33.0 37.0 8 35.38288113243653 37.0 37.0 37.0 33.0 37.0 9 37.059734537838125 39.0 39.0 39.0 34.0 39.0 10-11 37.057206031319126 39.0 38.5 39.0 34.5 39.0 12-13 37.0105369133215 39.0 38.0 39.0 34.0 39.0 14-15 38.5206907789681 41.0 39.0 41.0 34.5 41.0 16-17 38.51573009931809 41.0 39.0 41.0 34.0 41.0 18-19 38.52114599504553 41.0 39.0 41.0 34.0 41.0 20-21 38.56611833919933 41.0 39.0 41.0 34.0 41.0 22-23 38.53399458312461 41.0 39.0 41.0 34.0 41.0 24-25 38.545024266217155 41.0 39.0 41.0 34.0 41.0 26-27 38.50422723968596 41.0 39.0 41.0 34.0 41.0 28-29 38.44703884227514 41.0 39.0 41.0 34.0 41.0 30-31 38.3635844118863 41.0 39.0 41.0 34.0 41.0 32-33 38.27060669134978 41.0 39.0 41.0 33.5 41.0 34-35 38.21750936066633 40.0 38.5 41.0 33.0 41.0 36-37 38.38728405809785 41.0 39.0 41.0 34.0 41.0 38-39 38.353785510282854 41.0 39.0 41.0 34.0 41.0 40-41 38.27919832334187 41.0 39.0 41.0 33.0 41.0 42-43 38.213621083884505 41.0 39.0 41.0 33.0 41.0 44-45 38.126080158522306 41.0 38.5 41.0 33.0 41.0 46-47 38.03265114055466 41.0 38.0 41.0 33.0 41.0 48-49 37.955294450347814 40.5 38.0 41.0 33.0 41.0 50-51 37.847255086239606 40.0 38.0 41.0 33.0 41.0 52-53 37.75233632957383 40.0 38.0 41.0 33.0 41.0 54-55 37.61712703140991 40.0 38.0 41.0 32.0 41.0 56-57 37.44708651913834 40.0 38.0 41.0 32.0 41.0 58-59 37.2975558749978 40.0 37.0 41.0 32.0 41.0 60-61 37.099354011158994 40.0 37.0 41.0 31.0 41.0 62-63 36.860158535366764 40.0 36.5 41.0 31.0 41.0 64-65 36.58843372360873 39.5 36.0 41.0 31.0 41.0 66-67 36.30828480198754 39.0 35.5 41.0 30.5 41.0 68-69 35.94154424706804 39.0 35.0 41.0 30.0 41.0 70-71 35.55082647515807 38.0 35.0 40.0 30.0 41.0 72-73 35.16797016081472 37.0 35.0 40.0 29.5 41.0 74-75 34.741176678045974 37.0 35.0 39.0 29.0 41.0 76-77 34.27886360563789 36.0 34.5 39.0 29.0 40.5 78-79 33.821374060259636 36.0 34.0 38.5 28.5 39.5 80-81 33.38214671281091 35.0 34.0 37.0 28.0 39.0 82-83 33.14605937663481 35.0 34.0 37.0 29.0 39.0 84-85 32.70722617969214 35.0 34.0 36.5 28.0 38.0 86-87 32.0533784935548 35.0 34.0 36.0 14.5 37.0 88-89 16.474749222965094 18.0 16.5 18.5 2.0 19.0 90-91 2.802999527803122 2.0 2.0 2.0 2.0 2.0 92-93 2.3055348919531253 2.0 2.0 2.0 2.0 2.0 94-95 2.1041230036946303 2.0 2.0 2.0 2.0 2.0 96-97 2.000019593231453 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20319132625921554 1101 2 0.2275859392966666 1101 3 0.21334373253704797 1101 4 0.2083681947599274 1101 5 0.19769910185150508 1101 6 0.18127884645971903 1101 7 0.182740692900623 1101 8 0.22411004086854547 1101 9 0.29876969654703345 1101 10-11 0.23100537083573158 1101 12-13 0.23476184267229172 1101 14-15 0.2546428877240885 1101 16-17 0.26976008080819014 1101 18-19 0.2827714057733317 1101 20-21 0.26688401509323967 1101 22-23 0.2671112996770475 1101 24-25 0.3463056778340885 1101 26-27 0.3274949986501454 1101 28-29 0.3031501187707164 1101 30-31 0.2756530143933986 1101 32-33 0.2896795580782836 1101 34-35 0.3015823266764244 1101 36-37 0.22665707465044704 1101 38-39 0.21087655972581132 1101 40-41 0.21980926447702132 1101 42-43 0.25516293969374715 1101 44-45 0.1851086722574422 1101 46-47 0.21399367357718546 1101 48-49 0.2722240491491945 1101 50-51 0.195137866200497 1101 52-53 0.16880673084668274 1101 54-55 0.2776292190358376 1101 56-57 0.29887231709530937 1101 58-59 0.26981645345001937 1101 60-61 0.20347057301915328 1101 62-63 0.20461334450600788 1101 64-65 0.27837870848104984 1101 66-67 0.2622428195090549 1101 68-69 0.27665292371054306 1101 70-71 0.3379941018196462 1101 72-73 0.28489995587422356 1101 74-75 0.29851809085113956 1101 76-77 0.2828762130146458 1101 78-79 0.31017648495397054 1101 80-81 0.1558613469478516 1101 82-83 0.2550869574759247 1101 84-85 0.35008109205952564 1101 86-87 0.40531835439038844 1101 88-89 0.6812178981678834 1101 90-91 -0.16987673293538474 1101 92-93 -0.24856175535653557 1101 94-95 -0.059549736315695156 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.02975241645895821 1102 2 0.10210988357778916 1102 3 0.1288743531925931 1102 4 0.06366857665953063 1102 5 0.15074688051458196 1102 6 -0.01405791206433804 1102 7 0.1503169217106901 1102 8 0.15468563775839783 1102 9 0.21202760241839513 1102 10-11 0.18006875518059928 1102 12-13 0.13741348978606283 1102 14-15 0.20094604457767673 1102 16-17 0.18073779875197005 1102 18-19 0.14876607566370836 1102 20-21 0.2078222242863106 1102 22-23 0.19872950996910532 1102 24-25 0.18782558332080868 1102 26-27 0.19696072384728325 1102 28-29 0.2918410548368726 1102 30-31 0.27293333969493005 1102 32-33 0.26768082191870946 1102 34-35 0.12782789636623448 1102 36-37 0.21771809616321036 1102 38-39 0.20519191538699033 1102 40-41 0.27463389288045903 1102 42-43 0.22144972157137488 1102 44-45 0.22613557007868934 1102 46-47 0.18285165637796297 1102 48-49 0.17137617707182784 1102 50-51 0.1660384349286872 1102 52-53 0.2286962821833356 1102 54-55 0.21572741394201245 1102 56-57 0.19420171237513983 1102 58-59 0.20728064005881208 1102 60-61 0.10766311636063364 1102 62-63 0.17224232491647484 1102 64-65 0.13951913214233258 1102 66-67 0.16627271208261618 1102 68-69 0.15532072600193914 1102 70-71 0.2743414930881585 1102 72-73 0.29949698310174 1102 74-75 0.14002195105939563 1102 76-77 0.09861553218929942 1102 78-79 0.11017857303815504 1102 80-81 0.01889972923210337 1102 82-83 0.14367700610630862 1102 84-85 0.07797053349700889 1102 86-87 0.3356631630284639 1102 88-89 0.92357149873936 1102 90-91 -0.05762825091763624 1102 92-93 -0.0747277580490655 1102 94-95 0.06590576825885774 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.08703550162904605 1103 2 0.19470941232805217 1103 3 0.23753274104780076 1103 4 0.05761157006531903 1103 5 0.16470530369326752 1103 6 0.12193790624976941 1103 7 0.10978104514375531 1103 8 0.18405741918089547 1103 9 0.3076361287429137 1103 10-11 0.184775070408854 1103 12-13 0.1885812643706899 1103 14-15 0.15609524630330895 1103 16-17 0.2556289024582483 1103 18-19 0.2751334215061476 1103 20-21 0.2872004969575457 1103 22-23 0.2610707594993187 1103 24-25 0.25570240777206976 1103 26-27 0.29984563091583283 1103 28-29 0.3061727514659651 1103 30-31 0.34590328958334027 1103 32-33 0.3044971809740886 1103 34-35 0.36817453544492196 1103 36-37 0.3344522796334033 1103 38-39 0.26732126668207457 1103 40-41 0.35239851998753124 1103 42-43 0.3219452308849782 1103 44-45 0.3036432119519077 1103 46-47 0.3277672392334807 1103 48-49 0.2920092667506111 1103 50-51 0.2954081698491109 1103 52-53 0.2808775016470193 1103 54-55 0.2669292968944603 1103 56-57 0.3340564661543013 1103 58-59 0.29800889847915357 1103 60-61 0.2739244619229524 1103 62-63 0.2496207813465574 1103 64-65 0.2526002028295835 1103 66-67 0.2672304963689385 1103 68-69 0.34714107040692 1103 70-71 0.37155481811895186 1103 72-73 0.39126256225862477 1103 74-75 0.39184147260000657 1103 76-77 0.4363694876407891 1103 78-79 0.4365484343727175 1103 80-81 0.36987611323775127 1103 82-83 0.43560114653812576 1103 84-85 0.43449304470000527 1103 86-87 0.5194016362654352 1103 88-89 0.8414762813933798 1103 90-91 -0.5441241085629525 1103 92-93 -0.3311499906506534 1103 94-95 -0.11578503043334232 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.13447261659483445 1104 2 -0.07423580430417687 1104 3 -0.024857685288594666 1104 4 -0.015622497377094646 1104 5 -0.013050397580279594 1104 6 -0.11873219309870109 1104 7 -0.03476003765838698 1104 8 -0.006117785312525825 1104 9 0.03496949090819612 1104 10-11 -0.035991219888870774 1104 12-13 0.004764764908344432 1104 14-15 -0.05063488704727348 1104 16-17 -0.02995354005874873 1104 18-19 0.030690990166434062 1104 20-21 0.003225050863569834 1104 22-23 -0.09605467250759148 1104 24-25 0.03981452571547095 1104 26-27 0.03137551166491903 1104 28-29 -0.023621459777913856 1104 30-31 0.046529901670844254 1104 32-33 0.06021010626812284 1104 34-35 0.07363546808018384 1104 36-37 0.050001736158108656 1104 38-39 -0.01133394950088018 1104 40-41 0.0335770008263907 1104 42-43 0.06219145855298791 1104 44-45 0.04329320605256015 1104 46-47 0.051488559664093714 1104 48-49 -0.005830499257100996 1104 50-51 -0.049551997969643935 1104 52-53 -0.0622203810100217 1104 54-55 0.004398524734199327 1104 56-57 0.06770602021777705 1104 58-59 0.07305732146236465 1104 60-61 0.09206692563563479 1104 62-63 0.012110719905102485 1104 64-65 -0.06030054905453852 1104 66-67 -0.015359756387908874 1104 68-69 0.01811912859175635 1104 70-71 -0.10968964730096786 1104 72-73 -0.07537029684508667 1104 74-75 -0.12080319276810059 1104 76-77 -0.10838424480233755 1104 78-79 -0.06758573161619097 1104 80-81 -0.10806765353918735 1104 82-83 -0.08922095961916199 1104 84-85 -0.08775948372920794 1104 86-87 -0.13603561498024774 1104 88-89 0.6526724141048916 1104 90-91 -0.7228500540025422 1104 92-93 -0.3311499906506534 1104 94-95 -0.11578503043334232 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.07208497401590463 1105 2 -0.1284638445366859 1105 3 -0.020672774734009636 1105 4 0.013707117857002515 1105 5 0.019776921843643436 1105 6 -0.018774605656091126 1105 7 0.008288708097794029 1105 8 -0.09628298939478697 1105 9 -0.016981384286189893 1105 10-11 0.03716808536475469 1105 12-13 0.09760318945599522 1105 14-15 -0.009939321105989052 1105 16-17 0.05262186516558387 1105 18-19 0.06705176736117835 1105 20-21 0.10991978084719989 1105 22-23 -0.11742793784423355 1105 24-25 0.019225892690634794 1105 26-27 0.012771560945445515 1105 28-29 -0.11515513473381134 1105 30-31 -0.07041432479172727 1105 32-33 -0.10152075634592705 1105 34-35 -0.007910664283883762 1105 36-37 -0.11723497303722752 1105 38-39 -0.11241126943703961 1105 40-41 0.04902818233369999 1105 42-43 0.03173309559574733 1105 44-45 -0.1123125251747581 1105 46-47 0.05572510289724164 1105 48-49 0.04778542737531666 1105 50-51 -0.07435617284050977 1105 52-53 -0.10550219094029245 1105 54-55 -0.1595593044804744 1105 56-57 -0.10413357835863479 1105 58-59 -0.2737999490129681 1105 60-61 -0.3000609349069592 1105 62-63 -0.22157714416162122 1105 64-65 -0.18720598954070056 1105 66-67 -0.09122711367579939 1105 68-69 -0.06668416524530585 1105 70-71 -0.16973496931882437 1105 72-73 -0.18285426920111547 1105 74-75 -0.18457846358354857 1105 76-77 -0.08962529360137239 1105 78-79 -0.14715824582030024 1105 80-81 -0.20461133744884563 1105 82-83 -0.014273975315894916 1105 84-85 -0.10622861578798393 1105 86-87 -0.11583453438204572 1105 88-89 0.6712370256406999 1105 90-91 -0.8058767329353849 1105 92-93 -0.3311499906506534 1105 94-95 -0.11578503043334232 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.13818139400792973 1106 2 -0.1019254914765142 1106 3 -0.07792469797926316 1106 4 -0.15397652653470573 1106 5 -0.15654698086105157 1106 6 -0.2763805421865726 1106 7 -0.2636071108496978 1106 8 -0.1957101492162181 1106 9 -0.20556834609360664 1106 10-11 -0.2550105551853292 1106 12-13 -0.23290371088537398 1106 14-15 -0.15542030261755002 1106 16-17 -0.27954277046332976 1106 18-19 -0.3144281832325788 1106 20-21 -0.2897607318885278 1106 22-23 -0.2781232238266753 1106 24-25 -0.3556722312334628 1106 26-27 -0.5689894606357484 1106 28-29 -0.19376845302481627 1106 30-31 -0.18196887104200243 1106 32-33 -0.17182108409998875 1106 34-35 -0.28061906042863427 1106 36-37 -0.19869304094164164 1106 38-39 -0.31557502003273186 1106 40-41 -0.2573708023095236 1106 42-43 -0.5444877153280885 1106 44-45 -0.2990516154384295 1106 46-47 -0.420746629530953 1106 48-49 -0.3633115275268892 1106 50-51 -0.48052563238671553 1106 52-53 -0.5176047769878878 1106 54-55 -0.4856279091429414 1106 56-57 -0.5765769508214547 1106 58-59 -0.38709441220628094 1106 60-61 -0.3226599176050442 1106 62-63 -0.3698284741428566 1106 64-65 -0.30391463855208656 1106 66-67 -0.24722442939923894 1106 68-69 -0.30146986077442506 1106 70-71 -0.32201514554741806 1106 72-73 -0.2390733295098073 1106 74-75 -0.29398023948536434 1106 76-77 -0.2334952228959679 1106 78-79 -0.2065473783904963 1106 80-81 -0.25886970879322035 1106 82-83 -0.17612855755104562 1106 84-85 -0.1830558203473629 1106 86-87 -0.11405231090629542 1106 88-89 0.7656350571712238 1106 90-91 -0.2812042438524154 1106 92-93 -0.30669584217903756 1106 94-95 -0.11578503043334232 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.06790235565799563 1107 2 -0.09908944196694591 1107 3 0.0113742336263698 1107 4 -0.02018082484791961 1107 5 -0.038989351307535 1107 6 0.004843116612221365 1107 7 -0.055638392066697406 1107 8 0.035700454811902205 1107 9 0.0021596747605414635 1107 10-11 0.038892081075388774 1107 12-13 0.026622409121095814 1107 14-15 0.046390164412542845 1107 16-17 -0.026030768865020093 1107 18-19 -0.09723730882361536 1107 20-21 -0.1648894053860701 1107 22-23 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0.0 2216 1 -0.11836864592026686 2216 2 -0.1761013749697753 2216 3 -0.18860437798627316 2216 4 -0.06980391306564826 2216 5 -0.1449973805393725 2216 6 -0.158963360634516 2216 7 -0.09675702033730715 2216 8 -0.13854386770312033 2216 9 -0.3092129367069134 2216 10-11 -0.22532358568417266 2216 12-13 -0.21105955804851106 2216 14-15 -0.22936040015501646 2216 16-17 -0.32477158610436874 2216 18-19 -0.3482525156547851 2216 20-21 -0.3728186426090403 2216 22-23 -0.4330634637431885 2216 24-25 -0.3892666449681954 2216 26-27 -0.39925181358245254 2216 28-29 -0.38024432134141506 2216 30-31 -0.3725689595986381 2216 32-33 -0.5292950451632308 2216 34-35 -0.3905191760529334 2216 36-37 -0.36094913859801636 2216 38-39 -0.35477572598245644 2216 40-41 -0.43067966000714364 2216 42-43 -0.39144401121990313 2216 44-45 -0.5194215193247516 2216 46-47 -0.45056627752614986 2216 48-49 -0.27864449891495724 2216 50-51 -0.4121277564943 2216 52-53 -0.3640025089366503 2216 54-55 -0.3697676659094711 2216 56-57 -0.42135437795389663 2216 58-59 -0.4321987635289162 2216 60-61 -0.4678933488304935 2216 62-63 -0.4237911489287782 2216 64-65 -0.4060243517756632 2216 66-67 -0.4555185563418931 2216 68-69 -0.46251558009370086 2216 70-71 -0.4730869781221827 2216 72-73 -0.47683444250713336 2216 74-75 -0.46905170281336694 2216 76-77 -0.5516009088265719 2216 78-79 -0.5041199721455314 2216 80-81 -0.4895702293372679 2216 82-83 -0.5176002092574876 2216 84-85 -0.4715347740647857 2216 86-87 -0.6516449941880254 2216 88-89 -1.3337979300193563 2216 90-91 -0.8058767329353849 2216 92-93 -0.3311499906506534 2216 94-95 -0.11578503043334232 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16780.0 3 2933.0 4 2329.0 5 2147.0 6 2168.0 7 1982.0 8 2311.0 9 2676.0 10 3185.0 11 3753.0 12 4376.0 13 4902.0 14 5336.0 15 6044.0 16 6680.0 17 8078.0 18 10514.0 19 13299.0 20 14053.0 21 19577.0 22 23573.0 23 29523.0 24 44541.0 25 74749.0 26 140571.0 27 419856.0 28 640635.0 29 23715.0 30 855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.76384865926783 20.73166351106789 14.96165278050813 23.542835049156153 2 13.030237125366803 20.858894503474882 43.9002933125905 22.210575058567816 3 17.93131295707893 26.859138834386254 29.542824697959563 25.666723510575252 4 11.349019192732989 18.362831713846433 40.36184767217952 29.926301421241057 5 12.517846839647332 39.1945022912565 35.659504842905335 12.628146026190828 6 28.97538502332574 38.590044940341876 19.97967528790621 12.454894748426174 7 27.289714010662635 32.42986766078369 23.142545330573736 17.13787299797994 8 23.870891054448936 38.39215330266775 21.527540572684032 16.20941507019928 9 25.613251816782384 17.76668510607449 21.99692908752362 34.623133989619504 10-11 23.32188859701945 27.64896553255578 30.044032953525708 18.985112916899066 12-13 25.152846328128625 25.2654441572895 29.337286013507818 20.244423501074056 14-15 20.696859628492618 28.917463812106075 26.3426837706925 24.04299278870881 16-17 20.29676716522993 30.51139254395275 29.06370641871085 20.128133872106474 18-19 21.0863119585561 28.966283232861983 29.997253489228026 19.950151319353886 20-21 22.19115892268515 28.654963121357607 28.516598817976323 20.63727913798092 22-23 21.167780715701806 30.089153312936585 28.496404444701334 20.246661526660272 24-25 21.37432150272248 28.962780044424385 29.37322558797655 20.289672864876586 26-27 20.327781700052444 29.918766462396345 29.010522218397917 20.742929619153298 28-29 20.476109458542886 29.68845977630079 28.223615611724366 21.61181515343195 30-31 21.189622640893294 28.911021258003018 28.91552770123718 20.983828399866507 32-33 20.379748034201096 29.80758209428977 29.155412653674222 20.65725721783491 34-35 20.65391778220222 29.77453734650739 29.268248080434162 20.30329679085623 36-37 20.395607582201087 29.23958218018814 29.007336697496665 21.357473540114107 38-39 21.25121069842686 29.65504156704053 28.406724135791546 20.68702359874107 40-41 22.096103494060966 28.482092766113638 29.121942394593315 20.299861345232085 42-43 20.617271694768803 29.81750211117069 29.134906582737973 20.43031961132254 44-45 20.422293744913915 29.749473920704734 28.775427165938016 21.052805168443342 46-47 21.364622853153303 28.69755300132385 28.680866099203143 21.256958046319703 48-49 20.955407648041945 28.97092160923549 28.604788467655084 21.468882275067482 50-51 20.49197967136262 29.899019717654912 28.609164214518522 20.99983639646394 52-53 20.524399777682135 29.491829949038006 28.9921045808322 20.991665692447658 54-55 20.521260820141148 29.002043935969517 29.32605628122521 21.150638962664125 56-57 21.006393271423075 29.067734454240334 28.1098540238946 21.81601825044199 58-59 21.21806548188573 29.2061279790692 27.912386906235287 21.66341963280978 60-61 20.68411726940889 28.575389203215117 29.424167989754046 21.316325537621942 62-63 20.97596530168198 28.639566388584196 29.240719581253323 21.143748728480503 64-65 21.052243151093126 29.084958676174626 28.385446515778945 21.477351656953307 66-67 21.28321297646657 29.655694674755622 27.794664240589206 21.2664281081886 68-69 21.761739416099633 28.37774865630681 29.066388354517514 20.794123573076043 70-71 21.872218169326015 28.53199019554698 28.642659297870022 20.953132337256985 72-73 21.375921616624467 29.249820885209132 28.187443220448017 21.18681427771838 74-75 20.763502512178828 29.539702744554553 28.976397340284144 20.72039740298248 76-77 21.092407557501236 28.625449909577238 29.293089271334257 20.989053261587273 78-79 21.022542101121065 28.628925600943088 28.783941324572947 21.5645909733629 80-81 21.266754662046147 29.148785121683762 28.606640407382468 20.97781980888762 82-83 20.855140505842588 29.23745459639736 29.190136556141432 20.717268341618617 84-85 20.83699754333292 28.805376649221053 29.247465694667273 21.11016011277875 86-87 20.349181613756702 29.774975622467952 28.247893866136163 21.627948897639186 88-89 30.19139143353317 18.172066080240292 34.867325418061554 16.76921706816498 90-91 30.939952177280837 15.136076729053633 17.198941093683253 36.725029999982276 92-93 22.741843567459753 17.58353699299703 35.865781888719624 23.80883755082359 94-95 15.915812946824717 7.029185010632455 27.953041024073627 49.1019610184692 96-97 27.448217217438582 7.2621673460361595 15.18066074887576 50.1089546876495 98-99 57.26854713341199 14.120357363600608 18.911895263246613 9.699200239740781 100-101 30.871529199383296 7.133205507068954 12.64439571011814 49.350869583429606 102-103 23.463706203081568 2.0464893562230566 37.19795768658451 37.291846754110864 104-105 42.167218667964065 4.579052396327392 40.43953699536111 12.814191940347436 106-107 5.767772080463606 1.8111628886513191 6.664251306882169 85.7568137240029 108-109 18.60094338318713 11.104607052549515 21.756387186813704 48.538062377449656 110-111 40.401902505960756 2.712460898865913 25.3154252062184 31.570211388954927 112-113 27.63005244495461 2.7941559388764756 38.850641622508114 30.7251499936608 114-115 29.00712730152445 3.98807166897644 23.614135814690158 43.39066521480895 116-117 19.909192019338086 4.228356165184041 20.284052505789933 55.578399309687946 118-119 20.682213713887922 4.373361178791043 15.69439610163592 59.250029005685114 120-121 25.66771448747206 4.28833045919926 29.95621188572688 40.0877431676018 122-123 34.362627699365 1.606887897416363 34.11638050392199 29.914103899296652 124-125 38.55854937315446 1.940927111804774 19.786515664275882 39.71400785076488 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 69.0 2 171.0 3 282.0 4 376.5 5 407.5 6 375.5 7 319.5 8 256.5 9 220.5 10 195.5 11 192.5 12 183.5 13 163.5 14 168.0 15 195.5 16 245.5 17 343.5 18 468.5 19 721.0 20 1125.5 21 1654.5 22 2402.0 23 3532.0 24 5094.0 25 7234.0 26 10085.0 27 13425.5 28 17283.0 29 22085.5 30 27793.5 31 34420.0 32 42379.0 33 50830.0 34 58889.5 35 65419.0 36 68884.0 37 70296.5 38 70571.5 39 69943.5 40 69169.5 41 67955.0 42 66305.0 43 64603.0 44 62410.5 45 59789.0 46 57296.0 47 54604.0 48 51174.5 49 47280.0 50 43243.5 51 39196.0 52 34935.5 53 30417.0 54 26167.5 55 22162.0 56 18451.5 57 15189.5 58 12158.5 59 9562.5 60 7621.0 61 6008.0 62 4520.5 63 3308.0 64 2371.0 65 1670.5 66 1230.0 67 916.0 68 693.5 69 532.5 70 397.0 71 252.5 72 176.5 73 145.0 74 106.0 75 86.5 76 77.0 77 59.0 78 36.5 79 23.0 80 17.5 81 15.0 82 11.5 83 10.0 84 8.0 85 5.0 86 5.0 87 7.0 88 4.0 89 5.0 90 7.0 91 4.5 92 3.0 93 3.0 94 4.0 95 3.0 96 1.5 97 2.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.531077150961277E-4 3 0.04140702913709449 4 0.04689313394390197 5 0.04950556480428648 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.796615726441916E-4 12-13 0.0018287016022691576 14-15 3.265538575480638E-5 16-17 5.224861720769021E-4 18-19 0.007020907937283372 20-21 0.002481809317365285 22-23 9.470061868893851E-4 24-25 0.0 26-27 0.0 28-29 0.0014694923589662872 30-31 0.0 32-33 0.0013388708159470617 34-35 3.265538575480638E-5 36-37 1.6327692877403193E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.959323145288383E-4 46-47 0.0 48-49 2.6124308603845103E-4 50-51 3.265538575480638E-5 52-53 0.0 54-55 0.0017307354450047383 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.265538575480638E-5 64-65 1.6327692877403193E-4 66-67 0.0 68-69 4.2452001481248295E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.918646290576766E-4 80-81 0.0 82-83 8.163846438701596E-4 84-85 9.796615726441916E-5 86-87 0.0011755938871730299 88-89 19.33042090832915 90-91 26.30763593947259 92-93 40.40588032062364 94-95 44.762794543415666 96-97 48.46395596486542 98-99 93.02598519666053 100-101 51.66565326119541 102-103 53.08067643672268 104-105 74.89721717333676 106-107 81.70308939542473 108-109 12.58280589442775 110-111 39.14874593522086 112-113 52.09252446378224 114-115 86.80461172419783 116-117 66.47003770390839 118-119 71.85432301793237 120-121 80.43873816977013 122-123 75.82479340570202 124-125 50.035888268944525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1531141.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.20953245176864 #Duplication Level Percentage of deduplicated Percentage of total 1 74.79624906916133 46.53039683738562 2 14.592574387690965 18.15594459851818 3 5.157582495264836 9.625523868355549 4 2.26511825963594 5.636477915196628 5 1.0969407100249409 3.4120084348981345 6 0.6199276432481418 2.3139245304236233 7 0.38959210657160714 1.696543995770351 8 0.24612909495620197 1.2249260736001832 9 0.1649148664144945 0.9233349060592427 >10 0.6206222375156704 6.39746089416699 >50 0.033134907775076536 1.4122906884002544 >100 0.015744826434852092 1.8010708833508138 >500 0.0010495680757253907 0.4098450351800829 >1k 4.198272302901562E-4 0.4602513386943451 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2355 0.15380686690513806 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1931 0.12611509978506227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.531077150961277E-5 0.0 16-17 0.0 0.0 0.0 1.3062154301922554E-4 0.0 18-19 0.0 0.0 0.0 1.3062154301922554E-4 0.0 20-21 0.0 0.0 0.0 1.3062154301922554E-4 0.0 22-23 0.0 0.0 0.0 2.938984717932574E-4 0.0 24-25 0.0 0.0 0.0 4.5717540056728934E-4 0.0 26-27 0.0 0.0 0.0 7.837292581153532E-4 0.0 28-29 0.0 0.0 0.0 0.0013062154301922554 0.0 30-31 0.0 0.0 0.0 0.0038859909048219597 0.0 32-33 0.0 0.0 0.0 0.008849609539552529 0.0 34-35 0.0 0.0 0.0 0.015413342076268613 0.0 36-37 0.0 0.0 0.0 0.02671210554743162 0.0 38-39 0.0 0.0 0.0 0.045717540056728935 0.0 40-41 0.0 0.0 0.0 0.06919676241443473 0.0 42-43 0.0 0.0 0.0 0.09238208630034725 3.2655385754806385E-5 44-45 0.0 0.0 0.0 0.12069430574976439 6.531077150961277E-5 46-47 0.0 0.0 0.0 0.15459059616325344 6.531077150961277E-5 48-49 0.0 0.0 0.0 0.19106666205137215 6.531077150961277E-5 50-51 0.0 0.0 0.0 0.23133075268704842 6.531077150961277E-5 52-53 0.0 0.0 0.0 0.2728684033671621 6.531077150961277E-5 54-55 0.0 0.0 0.0 0.3181940787948334 6.531077150961277E-5 56-57 0.0 0.0 0.0 0.37214077606177354 6.531077150961277E-5 58-59 0.0 0.0 0.0 0.4312470242779731 6.531077150961277E-5 60-61 0.0 0.0 0.0 0.49874570663315787 6.531077150961277E-5 62-63 0.0 0.0 0.0 0.5695425829495782 6.531077150961277E-5 64-65 0.0 0.0 0.0 0.6414497423816617 6.531077150961277E-5 66-67 0.0 0.0 0.0 0.7208023297658412 6.531077150961277E-5 68-69 0.0 0.0 0.0 0.8137069022382655 6.531077150961277E-5 70-71 0.0 0.0 0.0 0.9255515984484772 6.531077150961277E-5 72-73 0.0 0.0 0.0 1.0385065777743527 6.531077150961277E-5 74-75 0.0 0.0 0.0 1.1511676586284345 6.531077150961277E-5 76-77 0.0 0.0 0.0 1.2746376721673576 6.531077150961277E-5 78-79 0.0 0.0 0.0 1.3104606303403803 6.531077150961277E-5 80-81 0.0 0.0 0.0 1.3112770149842503 6.531077150961277E-5 82-83 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 84-85 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 86-87 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 88-89 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 90-91 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 92-93 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 94-95 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 96-97 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 98-99 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 100-101 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 102-103 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 104-105 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 106-107 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 108-109 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 110-111 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 112-113 0.0 0.0 0.0 1.3113096703700051 6.531077150961277E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 310 0.0 2167.2834 108-109 CTCCGCC 180 0.0 1838.0525 110-111 CGTCGCG 40 6.37252E-6 1503.8612 110-111 CCGCGCG 110 0.0 1357.2886 108-109 CCGTTCG 60 2.277011E-8 1244.1813 108-109 CCGCGCC 415 0.0 1139.2502 108-109 CTCGTCG 30 0.008125914 1002.5742 110-111 CCGCACC 155 0.0 963.2371 108-109 CCGCTCG 165 0.0 904.8591 108-109 CGCCGTC 70 3.4127363E-5 859.3493 110-111 CCGCTAC 60 3.7994825E-5 829.45416 108-109 CCGCGCT 60 3.577768E-5 829.45416 108-109 CGTCGCT 75 4.1969968E-5 802.05927 110-111 CGTCGCC 165 1.8189894E-12 754.04926 108-109 GTTCGCT 80 5.092969E-5 751.9306 110-111 CAGCGCG 100 1.7547427E-7 746.5087 108-109 CTCCGTC 170 7.6579454E-10 707.69934 110-111 TTGTTCG 325 0.0 689.08496 108-109 CGACGCC 110 2.568304E-7 678.6443 108-109 CGTCTCT 240 5.456968E-12 626.6089 110-111 >>END_MODULE