FastQCFastQC Report
Fri 27 May 2016
SRR939947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531141
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39510.25804285823448003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.17816778467822364No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22770.14871262672738828No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18790.12271893966656237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.11749407794579338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69800.040.5395851
ATCAACG79550.034.6699753
TCAACGC80300.034.346164
TAGGACC18250.034.271984
CAACGCA81900.033.675175
AACGCAG83300.033.210966
TATCAAC84200.033.179782
GGTATCA46250.030.1401391
CGCAGAG93900.029.3985488
ACGCAGA94000.029.3672737
GCAGAGT104500.026.4734239
GTCCTAC45900.025.827451
AGTACTT53450.025.71210912-13
GTAGGAC53300.025.704753
GTACATG71300.025.4830531
GAGTACT51200.025.33144812-13
TACATGG73150.024.5112192
TACAGCG2201.5224941E-724.3687115
CTACAGT48800.023.9248354
TCCTACA54050.023.8051432
Produced by FastQC (version 0.11.5)