##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939947_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531141 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8394628580908 34.0 31.0 34.0 31.0 34.0 2 32.91091349523003 34.0 31.0 34.0 31.0 34.0 3 32.95505573947794 34.0 33.0 34.0 31.0 34.0 4 36.368591788737945 37.0 37.0 37.0 35.0 37.0 5 36.27120755044767 37.0 37.0 37.0 35.0 37.0 6 36.40007092749786 37.0 37.0 37.0 35.0 37.0 7 36.39167653403573 37.0 37.0 37.0 35.0 37.0 8 36.41253287580961 37.0 37.0 37.0 35.0 37.0 9 38.258226381502425 39.0 39.0 39.0 37.0 39.0 10-11 38.248062719240096 39.0 39.0 39.0 37.0 39.0 12-13 38.23314541247344 39.0 39.0 39.0 37.0 39.0 14-15 39.7637673473573 41.0 40.0 41.0 37.0 41.0 16-17 39.7740619577165 41.0 40.0 41.0 37.0 41.0 18-19 39.82012891040081 41.0 40.0 41.0 37.5 41.0 20-21 39.84286195719402 41.0 40.0 41.0 38.0 41.0 22-23 39.79408297472277 41.0 40.0 41.0 37.5 41.0 24-25 39.76979618467534 41.0 40.0 41.0 37.0 41.0 26-27 39.686664062943905 41.0 40.0 41.0 37.0 41.0 28-29 39.60525777834961 41.0 40.0 41.0 37.0 41.0 30-31 39.48865584554264 41.0 39.5 41.0 36.5 41.0 32-33 39.356930877038764 41.0 39.0 41.0 36.0 41.0 34-35 39.282673509493904 41.0 39.0 41.0 35.0 41.0 36-37 39.27709498994541 41.0 39.5 41.0 35.5 41.0 38-39 39.34041215015469 41.0 40.0 41.0 36.0 41.0 40-41 39.272615977235276 41.0 40.0 41.0 35.0 41.0 42-43 39.190285871777974 41.0 40.0 41.0 35.0 41.0 44-45 39.056600600467235 41.0 40.0 41.0 35.0 41.0 46-47 38.87445506325022 41.0 39.0 41.0 35.0 41.0 48-49 38.697838735949205 41.0 39.0 41.0 35.0 41.0 50-51 38.56647069081163 41.0 39.0 41.0 35.0 41.0 52-53 38.46975294894461 41.0 39.0 41.0 35.0 41.0 54-55 38.37406842348288 41.0 39.0 41.0 35.0 41.0 56-57 38.2489519253942 41.0 38.5 41.0 35.0 41.0 58-59 38.10659828193485 41.0 38.0 41.0 34.0 41.0 60-61 37.94892860944877 40.0 38.0 41.0 34.0 41.0 62-63 37.748169502351516 40.0 37.0 41.0 34.0 41.0 64-65 37.52421560130648 40.0 37.0 41.0 34.0 41.0 66-67 37.26883187113401 39.0 36.5 41.0 33.5 41.0 68-69 36.951013655829215 39.0 36.0 41.0 33.0 41.0 70-71 36.588342941636334 39.0 35.0 41.0 33.0 41.0 72-73 36.24204400509163 37.5 35.0 40.0 32.5 41.0 74-75 35.86107451893719 37.0 35.0 39.0 32.0 41.0 76-77 35.45478567943775 36.5 35.0 39.0 32.0 41.0 78-79 35.0631924166357 36.0 35.0 39.0 31.5 40.0 80-81 34.746646455159905 36.0 35.0 37.0 31.5 39.0 82-83 34.4723376227271 35.0 35.0 37.0 32.0 39.0 84-85 34.16802142977035 35.0 35.0 37.0 32.0 38.5 86-87 33.92430775480508 35.0 35.0 36.0 31.0 37.0 88-89 33.695373254324714 35.0 35.0 36.0 31.0 37.0 90-91 33.512950472882636 35.0 35.0 36.0 31.0 37.0 92-93 33.36952736553982 35.0 35.0 35.0 31.0 36.0 94-95 33.26476562249982 35.0 35.0 35.0 31.0 36.0 96-97 33.16146455486464 35.0 34.0 35.0 31.0 36.0 98-99 33.06772596384004 35.0 34.0 35.0 31.0 36.0 100-101 33.03451282409654 35.0 34.0 35.0 31.0 36.0 102-103 32.21221135088147 34.5 33.0 35.0 28.0 35.0 104-105 32.87769806960952 35.0 34.0 35.0 30.5 35.0 106-107 32.946725350571896 35.0 34.0 35.0 31.0 35.0 108-109 32.944314403441616 35.0 34.0 35.0 31.0 35.0 110-111 32.92052005661138 35.0 34.0 35.0 31.0 35.0 112-113 32.8579644199979 35.0 34.0 35.0 31.0 35.0 114-115 32.807430537096195 35.0 34.0 35.0 31.0 35.0 116-117 32.75258222462856 35.0 34.0 35.0 30.5 35.0 118-119 32.68317189599129 35.0 34.0 35.0 30.0 35.0 120-121 32.59423593254965 35.0 34.0 35.0 30.0 35.0 122-123 32.43881523648051 35.0 34.0 35.0 30.0 35.0 124-125 30.446937610579297 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07572591185137867 1101 2 0.0686843369009793 1101 3 0.10599940709872158 1101 4 0.07192312287649116 1101 5 -0.02599577047229218 1101 6 0.05690471797107932 1101 7 0.06082186989886651 1101 8 0.0881603449422741 1101 9 0.10746110511691853 1101 10-11 0.08553682752927472 1101 12-13 0.06431306428702754 1101 14-15 0.10084965565289394 1101 16-17 0.05613994834062197 1101 18-19 0.12270078644077387 1101 20-21 0.17113019059430457 1101 22-23 0.1593629851243321 1101 24-25 0.18594101399985874 1101 26-27 0.17518401951124218 1101 28-29 0.23274509294025592 1101 30-31 0.16741253561269787 1101 32-33 0.2130136611968112 1101 34-35 0.2828647446043817 1101 36-37 0.24228395372165323 1101 38-39 0.17614133760007178 1101 40-41 0.19341898033240312 1101 42-43 0.14255665734830814 1101 44-45 0.13710077100931528 1101 46-47 0.20201518180147815 1101 48-49 0.20520073951696816 1101 50-51 0.17867261741302087 1101 52-53 0.14404915214333158 1101 54-55 0.08868318040818934 1101 56-57 0.03390747934177085 1101 58-59 0.053405237905543856 1101 60-61 0.03499199854211099 1101 62-63 0.04773852845112714 1101 64-65 0.08622047757277329 1101 66-67 0.1083421449453752 1101 68-69 0.042294639931689915 1101 70-71 0.14498277573662222 1101 72-73 0.09336594161575817 1101 74-75 0.24022182235062672 1101 76-77 0.21228034810996377 1101 78-79 0.23134311419376985 1101 80-81 0.20208909397241825 1101 82-83 0.17741656841809572 1101 84-85 0.17658797438070906 1101 86-87 0.15354844129765155 1101 88-89 0.16715014183795773 1101 90-91 0.12203563932873607 1101 92-93 0.07853209489753965 1101 94-95 0.10796315945629686 1101 96-97 0.07711001627157543 1101 98-99 0.08088624556334878 1101 100-101 0.09669251160102732 1101 102-103 0.15891849448915707 1101 104-105 0.09936591116958482 1101 106-107 0.08663271826439711 1101 108-109 0.10207540851764918 1101 110-111 0.094928189973011 1101 112-113 0.16416675979341022 1101 114-115 0.1189589495192962 1101 116-117 0.1353785828020122 1101 118-119 0.06691097198508089 1101 120-121 0.124081549910116 1101 122-123 0.06543350414378324 1101 124-125 0.14021811479587498 1102 1 0.047326648175804564 1102 2 0.10378151523985224 1102 3 0.11990187158755106 1102 4 0.05316179639353891 1102 5 -0.06432991889111861 1102 6 -0.029245019644662307 1102 7 -0.02154909474107569 1102 8 -0.010442726739455566 1102 9 0.03645876975961215 1102 10-11 0.12058466114193322 1102 12-13 0.10987209387737806 1102 14-15 0.09545151844378097 1102 16-17 0.1992954831374476 1102 18-19 0.08448565882036974 1102 20-21 0.04627440577687736 1102 22-23 0.0837364224955337 1102 24-25 0.11705533660886402 1102 26-27 0.12799002000578952 1102 28-29 0.1334149283662498 1102 30-31 0.02695568477334831 1102 32-33 0.125498316525551 1102 34-35 0.1368198507944527 1102 36-37 0.24394299155444088 1102 38-39 0.19797060924334176 1102 40-41 0.1625556674219979 1102 42-43 0.15476722415326094 1102 44-45 0.16482448450218357 1102 46-47 0.16868891864792346 1102 48-49 0.10667602594166681 1102 50-51 0.08971396697480571 1102 52-53 0.1763390661472286 1102 54-55 0.17167309715956947 1102 56-57 0.19647527339072468 1102 58-59 0.20586901239244781 1102 60-61 0.1519339443068688 1102 62-63 0.11090769074472462 1102 64-65 0.12209607171514847 1102 66-67 0.2886875580288688 1102 68-69 0.1858385583865143 1102 70-71 0.1885725221442911 1102 72-73 0.18314378044863133 1102 74-75 0.15985992439200203 1102 76-77 0.1951778396614685 1102 78-79 0.20467135553234073 1102 80-81 0.18898185985971594 1102 82-83 0.19876514110265475 1102 84-85 0.19714428396666506 1102 86-87 0.23753272571669726 1102 88-89 0.2166050969166733 1102 90-91 0.2411639191320205 1102 92-93 0.21556195999630745 1102 94-95 0.0949515376193375 1102 96-97 0.06696896069007607 1102 98-99 0.1458374228581789 1102 100-101 0.21411394743365264 1102 102-103 0.15120082545050195 1102 104-105 0.19156537815862862 1102 106-107 0.21391815387722346 1102 108-109 0.13980939758268107 1102 110-111 0.20250903454810754 1102 112-113 0.23364962926066823 1102 114-115 0.311293152887707 1102 116-117 0.26606319966603564 1102 118-119 0.2347765653032141 1102 120-121 0.31624573926638533 1102 122-123 0.2584515001324661 1102 124-125 0.16868562585447933 1103 1 0.009517948793956066 1103 2 0.04385098119447406 1103 3 0.03384347507735441 1103 4 0.0228277326437194 1103 5 -0.10075744255325958 1103 6 0.018695144045658196 1103 7 0.014110564290362504 1103 8 -0.0293432392389974 1103 9 0.09293988193115865 1103 10-11 0.06338567441627418 1103 12-13 -0.011201420100974246 1103 14-15 -0.014388511179333818 1103 16-17 0.0298031158440466 1103 18-19 0.04342685833918836 1103 20-21 0.042967492927381556 1103 22-23 0.023766024295269972 1103 24-25 0.09060898725945066 1103 26-27 0.08484869049146226 1103 28-29 0.12577486715024833 1103 30-31 0.06873054780160714 1103 32-33 0.01645834130528101 1103 34-35 0.07570991334280563 1103 36-37 0.09447564701239486 1103 38-39 0.03073419071576211 1103 40-41 0.07759939754721046 1103 42-43 0.05761902478730008 1103 44-45 0.02529471951523732 1103 46-47 0.11480611798426565 1103 48-49 0.17938110303617805 1103 50-51 0.185593228468953 1103 52-53 0.1656362866776533 1103 54-55 0.14724585659687506 1103 56-57 0.07803130139455305 1103 58-59 0.04125456805294192 1103 60-61 0.06490409884548853 1103 62-63 0.026919885338926974 1103 64-65 -0.015090661579371556 1103 66-67 0.10708384226327894 1103 68-69 0.09631155404558456 1103 70-71 0.16932932713673665 1103 72-73 0.20984093289022354 1103 74-75 0.10128359913802143 1103 76-77 0.15144377066748405 1103 78-79 0.12203573373360399 1103 80-81 0.07925332196393242 1103 82-83 0.14822560001795182 1103 84-85 0.037545581704783615 1103 86-87 0.04633379313806785 1103 88-89 0.03585398375810911 1103 90-91 0.0825428479629906 1103 92-93 0.06786750446072176 1103 94-95 0.10355249551020762 1103 96-97 0.03429303772296777 1103 98-99 0.09318060484060453 1103 100-101 0.10825553681110733 1103 102-103 1.567150169847764E-4 1103 104-105 -0.040457859872375934 1103 106-107 -0.054849602459420055 1103 108-109 -0.055357297472099276 1103 110-111 0.03687304459216989 1103 112-113 0.06171201176000807 1103 114-115 0.1489959457874548 1103 116-117 0.0837617546010847 1103 118-119 0.10597462811846015 1103 120-121 0.12259686657991864 1103 122-123 0.1740483187597306 1103 124-125 0.09987210035175664 1104 1 0.005195255430578527 1104 2 0.035307206103858846 1104 3 -0.0056756835447089315 1104 4 -0.01361137574356519 1104 5 0.015184163506823722 1104 6 8.765779202093427E-4 1104 7 -0.08753529228523149 1104 8 -0.04377817986966903 1104 9 -0.16458305311675048 1104 10-11 -0.021514311601627867 1104 12-13 0.029729699386329855 1104 14-15 -0.04038807366146102 1104 16-17 -0.01578093482405052 1104 18-19 -0.04446258820070881 1104 20-21 0.06626888857579871 1104 22-23 0.056919129937355706 1104 24-25 0.07558201188935243 1104 26-27 0.09974487237125373 1104 28-29 0.10102854983726672 1104 30-31 0.04444830594659521 1104 32-33 0.004781505777145867 1104 34-35 0.038559208590527305 1104 36-37 0.10733823155054267 1104 38-39 0.06813441081831684 1104 40-41 -0.009528906458008635 1104 42-43 -0.03610311861359605 1104 44-45 0.0013477540711690494 1104 46-47 -0.008030383434736166 1104 48-49 -0.015004791085331703 1104 50-51 0.0033603169638709574 1104 52-53 0.011243913764623414 1104 54-55 -0.033401997361167446 1104 56-57 0.0030405493130913896 1104 58-59 -0.09511643534516878 1104 60-61 -0.06820882401322592 1104 62-63 -0.03945634140113441 1104 64-65 -0.043575615289398684 1104 66-67 -0.062085150903968156 1104 68-69 0.004347564873519616 1104 70-71 -0.0340663070059577 1104 72-73 -0.00860613479530059 1104 74-75 -0.07475593618609366 1104 76-77 -0.0823438198644908 1104 78-79 -0.08080061652758985 1104 80-81 -0.05395008563686332 1104 82-83 0.030442597965148366 1104 84-85 -0.007777196894792837 1104 86-87 -0.03947975286870076 1104 88-89 -0.032344744248952395 1104 90-91 -0.05089936797684658 1104 92-93 -0.07938056271380844 1104 94-95 -0.017354119617237984 1104 96-97 0.0031136961243873884 1104 98-99 -0.00396704736376563 1104 100-101 -0.0343716152456679 1104 102-103 0.029458025849081082 1104 104-105 0.05162999472389629 1104 106-107 0.035585908372091524 1104 108-109 0.03690564824923115 1104 110-111 0.008672981357825904 1104 112-113 0.03917763700538046 1104 114-115 -0.029871290212291512 1104 116-117 -0.1314109620867825 1104 118-119 -0.06880914273973815 1104 120-121 -0.19328410840472543 1104 122-123 -0.09131025904686396 1104 124-125 -0.14186565597094258 1105 1 -0.07350401085266611 1105 2 -0.06357715923422802 1105 3 -0.08119933520443823 1105 4 -0.01883856977384113 1105 5 -0.15274436211384312 1105 6 -0.07844023159003655 1105 7 -0.13524573130642636 1105 8 -0.019554053066372035 1105 9 -0.08457452962695555 1105 10-11 -0.06500634029465147 1105 12-13 -0.01461160491249558 1105 14-15 0.012016431494636493 1105 16-17 -0.03307163295113469 1105 18-19 -0.012884697641496246 1105 20-21 0.007267489166295604 1105 22-23 -0.03868758476954781 1105 24-25 -0.05264774533461036 1105 26-27 -0.036093328844579275 1105 28-29 -0.014691039641952841 1105 30-31 -0.12162349216143298 1105 32-33 -0.03655321945299761 1105 34-35 -0.03600647247933608 1105 36-37 -0.07079399728057467 1105 38-39 -0.0430018564258674 1105 40-41 -0.02031716535081074 1105 42-43 -0.08736709478060334 1105 44-45 -0.07406914907452489 1105 46-47 -0.005172555654304745 1105 48-49 0.036242793755143055 1105 50-51 0.014884853755226857 1105 52-53 -0.061743589897808704 1105 54-55 0.02058099253972756 1105 56-57 0.08254104412625196 1105 58-59 -0.011767564400237518 1105 60-61 0.10461128322702962 1105 62-63 0.06281150107002276 1105 64-65 0.042711503381759997 1105 66-67 0.004064403559290497 1105 68-69 -0.02075370279069233 1105 70-71 0.07546934720262755 1105 72-73 -0.03030548508409936 1105 74-75 -0.0353510847632279 1105 76-77 -0.020736814211531396 1105 78-79 0.05360068002634222 1105 80-81 0.01078868784114917 1105 82-83 0.020759421817807322 1105 84-85 0.05535543147490074 1105 86-87 -0.05510294085877376 1105 88-89 -0.036692646056714295 1105 90-91 -0.015424337089449125 1105 92-93 -0.14413736665100885 1105 94-95 -0.13521462123281935 1105 96-97 -0.10477862515381275 1105 98-99 -0.10209659211515287 1105 100-101 0.05225808214996164 1105 102-103 0.00405146972825321 1105 104-105 0.05437390278494547 1105 106-107 -0.05796154349375371 1105 108-109 -0.024355090630791665 1105 110-111 -0.07378922126765985 1105 112-113 -0.12727146895019814 1105 114-115 -0.07607157583115765 1105 116-117 -0.14815449724515872 1105 118-119 -0.03498133772276901 1105 120-121 -0.23151913134233837 1105 122-123 -0.148314170427156 1105 124-125 -0.1756540193584506 1106 1 -0.04814051506873085 1106 2 0.0068831552929609074 1106 3 -0.022268766546282848 1106 4 0.007765917636312736 1106 5 -0.009841647687807153 1106 6 0.05830518033943122 1106 7 -0.002872966987851555 1106 8 -0.06626143774459337 1106 9 -0.05063496475207785 1106 10-11 0.0107189492862787 1106 12-13 0.030413244096941128 1106 14-15 -0.14214905999057237 1106 16-17 -0.0291264272052274 1106 18-19 -0.041042855994362526 1106 20-21 0.0028938689914284055 1106 22-23 0.01963269999717454 1106 24-25 -0.016539849087735092 1106 26-27 -0.05006129258739378 1106 28-29 0.015090327977496543 1106 30-31 0.04774852328287693 1106 32-33 0.09046703905450926 1106 34-35 0.05199549209988419 1106 36-37 0.05424028559938421 1106 38-39 -0.037709163298977444 1106 40-41 -0.01734598242125429 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-0.012715495469130644 2216 9 -0.049262649809627135 2216 10-11 -0.05021388379072533 2216 12-13 -0.09257752942806263 2216 14-15 -0.06114954345348167 2216 16-17 -0.0913355084848888 2216 18-19 -0.11543473319166253 2216 20-21 -0.11144354430989978 2216 22-23 -0.11695754093632615 2216 24-25 -0.07634178575565898 2216 26-27 -0.11611406045165751 2216 28-29 -0.28744206325507804 2216 30-31 -0.29286752424356166 2216 32-33 -0.2486634943662338 2216 34-35 -0.19732359185524473 2216 36-37 -0.11910909536469916 2216 38-39 -0.11764920763987874 2216 40-41 -0.10860500432416131 2216 42-43 -0.1003643281723754 2216 44-45 -0.07871772120304854 2216 46-47 -0.06016948740837336 2216 48-49 0.002719992578278152 2216 50-51 -0.055062455405071375 2216 52-53 -0.11149585597099332 2216 54-55 -0.2229027154334844 2216 56-57 -0.28261453469495024 2216 58-59 -0.22093615354175 2216 60-61 -0.20434376557363976 2216 62-63 -0.17276291526501808 2216 64-65 -0.20699376147290138 2216 66-67 -0.12339364446174983 2216 68-69 -0.18302481124541004 2216 70-71 -0.20881333360643595 2216 72-73 -0.10441802786598231 2216 74-75 -0.16293816668977712 2216 76-77 -0.18503623671099945 2216 78-79 -0.2697859772134805 2216 80-81 -0.22212453386496378 2216 82-83 -0.17118030809675133 2216 84-85 -0.21089936939234377 2216 86-87 -0.13470632718085795 2216 88-89 -0.16491907223247892 2216 90-91 -0.1586941855284678 2216 92-93 -0.14354989131866347 2216 94-95 -0.1177030365097238 2216 96-97 -0.05205945703548309 2216 98-99 -0.03826295248921241 2216 100-101 -0.09640741041722123 2216 102-103 -0.08247506042474129 2216 104-105 -0.19511247524050646 2216 106-107 -0.14825024588507318 2216 108-109 -0.13641166126548399 2216 110-111 -0.12812869455077447 2216 112-113 -0.1161530075259094 2216 114-115 -0.1902157506725004 2216 116-117 -0.253819301152717 2216 118-119 -0.1420294457441713 2216 120-121 -0.20568218646941716 2216 122-123 -0.30247856616624347 2216 124-125 -0.2548241007288148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 156.0 3 5.0 4 23.0 5 34.0 6 108.0 7 279.0 8 507.0 9 958.0 10 2037.0 11 2719.0 12 3354.0 13 5370.0 14 9230.0 15 9634.0 16 3677.0 17 2437.0 18 2194.0 19 2093.0 20 2046.0 21 2210.0 22 2280.0 23 2453.0 24 2989.0 25 3976.0 26 5083.0 27 6632.0 28 8626.0 29 11226.0 30 14042.0 31 18153.0 32 23653.0 33 31531.0 34 45418.0 35 73720.0 36 160089.0 37 549927.0 38 517864.0 39 4408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86249918348684 20.61734927167026 15.319746554314456 23.200404990528448 2 13.338222933093686 21.06572810733956 43.36609103929684 22.229957920269918 3 17.840747520966392 27.055640205572185 29.71796849539004 25.385643778071387 4 11.366098876589419 18.511750387456154 40.19316313781683 29.928987598137596 5 12.30182317772141 38.863811285640764 35.66878730988267 13.165578226755153 6 28.813218377667372 38.89576466177837 19.51205016389738 12.77896679665687 7 26.776632589683118 32.710965221361064 23.174874162470992 17.337528026484826 8 24.010198930078943 37.88801945738505 21.332718541270857 16.769063071265155 9 25.70004983211866 17.094310713383027 22.088494789180096 35.117144665318214 10-11 23.284857501693182 27.45041116396204 29.872559091553292 19.392172242791485 12-13 25.59065428984006 25.029798039501262 29.42906629761727 19.950481373041413 14-15 20.822282206537476 28.388469775154608 27.20206695529674 23.58718106301118 16-17 19.873989274001488 30.11603449587709 29.772466845770744 20.237509384350673 18-19 20.573807376329157 27.950495741411142 31.67360158208813 19.802095300171572 20-21 21.59326668950466 27.696758061087163 30.4893066927834 20.220668556624776 22-23 20.604315221750095 29.061390939288295 30.502769835246724 19.831524003714883 24-25 20.80458298745837 28.062699646864658 31.314359683399505 19.818357682277465 26-27 19.80637315570545 28.98263451896331 30.947476424444254 20.263515900886986 28-29 19.946366794436308 28.641810257840394 30.272293668577877 21.13952927914542 30-31 20.710404854941512 27.807432496419338 30.943263879681883 20.538898768957267 32-33 19.835697692113268 28.80766696208906 31.143637326673378 20.2129980191243 34-35 20.212900052967036 28.83362799376413 31.153368631628307 19.800103321640528 36-37 19.972752346126192 28.259546312194633 30.8805328836469 20.887168458032278 38-39 20.82045350493521 28.70271255227311 30.216714202023198 20.260119740768488 40-41 21.70436948654631 27.573358691328885 30.80294368709348 19.919328135031325 42-43 20.283696929283458 28.825072282696368 30.798306622316296 20.092924165703877 44-45 20.137694699573714 28.876733102960472 30.23020087633993 20.755371321125878 46-47 21.20170513362257 27.93929494409724 29.902340803361678 20.956659118918505 48-49 20.95381810035784 28.304349501450226 29.558740834449605 21.183091563742334 50-51 20.507941463261712 29.29488531755077 29.35346907959489 20.84370413959263 52-53 20.475416699049926 28.972478693993565 29.727863077273746 20.824241529682766 54-55 20.50196552766858 28.49175876029706 29.99557192969165 21.01070378234271 56-57 20.938927244453645 28.565723209031695 28.84378381873387 21.651565727780785 58-59 21.18429981301526 28.618461657025705 28.652488568982214 21.544749960976812 60-61 20.69525275595128 28.03771827676223 30.09791390864721 21.169115058639278 62-63 20.90728417565724 28.132059686207867 29.93212251517006 21.028533622964833 64-65 21.006589203737605 28.483823501558643 29.206421877540993 21.303165417162756 66-67 21.225837463695374 29.118807477560853 28.60850176437049 21.04685329437328 68-69 21.713219096085858 27.84273296842028 29.806693178485848 20.63735475700801 70-71 21.777027719850754 28.02099871925577 29.349615744075823 20.852357816817655 72-73 21.324554694832155 28.730959460951016 28.9540284010421 20.99045744317473 74-75 20.75122408713502 29.109827246478282 29.619969682739868 20.518978983646836 76-77 21.06595669503984 28.08539513996425 29.93401652754384 20.914631637452068 78-79 20.86639963269222 28.148028169841965 29.516843974526186 21.468728222939625 80-81 21.120458533864614 28.65898699074742 29.3361943805306 20.884360094857364 82-83 20.79723552566354 28.719595386708342 29.811166966334255 20.672002121293858 84-85 20.729018424821753 28.3170851018946 29.803460295296127 21.150436177987526 86-87 20.202548295682764 29.265789368843237 28.922352676859937 21.609309658614066 88-89 20.999470329643056 28.162951681131915 29.401374530497193 21.43620345872784 90-91 20.848964551532077 28.530543574524337 29.731785276698687 20.888706597244898 92-93 20.374478901681815 29.122040360750578 29.735798336012166 20.76768240155544 94-95 20.30345541955587 29.000947665483878 29.475368208206877 21.220228706753375 96-97 21.14739922711233 28.425990813386882 29.212757022377428 21.213852937123363 98-99 21.072450355477283 28.14411500672224 29.692305029584737 21.091129608215738 100-101 20.733787309247333 28.345966089938955 29.711355308484507 21.208891292329206 102-103 20.442632859904116 28.82168667852934 29.44357889106339 21.29210157050316 104-105 20.706789501579415 28.266159146995978 29.33623493858853 21.690816412836075 106-107 20.67952800957253 27.880804520824952 29.3265462611231 22.11312120847942 108-109 20.455773004993027 28.55848765784859 29.033853971073746 21.951885366084635 110-111 20.272581859792837 28.68572357897748 29.442387413189397 21.599307148040285 112-113 20.49505196239609 27.93160859066329 29.226177996108454 22.34716145083216 114-115 20.298669188669148 28.341340321091586 28.62744957165255 22.732540918586714 116-117 20.62466201123886 28.23750780476463 28.07883269458356 23.058997489412953 118-119 20.656249806108455 27.83815583100038 28.92545062027333 22.580143742617835 120-121 20.160718058608406 27.824019995709047 29.532694349298573 22.482567596383973 122-123 20.62653380118181 28.31135327186427 28.77382443272828 22.288288494225643 124-125 20.83235229670336 28.19951558288924 28.33418837274842 22.633943747658982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 192.0 1 131.5 2 134.0 3 189.0 4 125.5 5 82.5 6 210.5 7 559.5 8 970.0 9 1077.5 10 768.5 11 498.5 12 517.5 13 592.5 14 706.5 15 839.0 16 954.5 17 1099.0 18 1282.5 19 1490.5 20 1832.5 21 2338.5 22 2929.5 23 3681.0 24 4568.5 25 5931.0 26 8126.5 27 10634.5 28 13345.5 29 17165.5 30 22032.0 31 27305.0 32 34104.5 33 42386.5 34 52411.0 35 62820.0 36 71045.5 37 75437.0 38 73921.0 39 68757.5 40 63635.5 41 61168.0 42 59286.5 43 57488.5 44 57651.5 45 58376.0 46 57680.0 47 56290.0 48 55874.0 49 53544.0 50 50151.5 51 45752.5 52 39693.5 53 35117.0 54 31272.0 55 26738.5 56 22562.0 57 19043.0 58 15769.0 59 12722.5 60 10107.5 61 7764.0 62 5737.0 63 4047.0 64 2738.5 65 2041.5 66 1516.5 67 1125.0 68 850.0 69 578.5 70 377.5 71 287.5 72 257.0 73 260.0 74 177.0 75 119.5 76 83.5 77 47.5 78 33.5 79 17.0 80 11.0 81 8.5 82 7.5 83 2.5 84 2.0 85 3.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 2.5 98 7.5 99 9.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015739895933816677 2 0.0 3 0.0 4 0.0 5 0.1233067366101489 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.796615726441916E-5 18-19 0.0 20-21 0.004343166305389249 22-23 1.959323145288383E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.001110283115663417 92-93 0.0 94-95 6.531077150961277E-4 96-97 0.0 98-99 0.0019919785310431896 100-101 1.6327692877403193E-4 102-103 4.898307863220958E-4 104-105 0.0022532216170816403 106-107 0.006073901750393987 108-109 3.918646290576766E-4 110-111 0.005094240177749796 112-113 0.008490400296249659 114-115 0.005388138649543053 116-117 0.0016327692877403193 118-119 9.796615726441916E-5 120-121 8.163846438701596E-4 122-123 0.0012082492729278361 124-125 0.0014694923589662872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1531141.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.10400445426984 #Duplication Level Percentage of deduplicated Percentage of total 1 73.25688241627951 44.030319870539856 2 15.479173229160093 18.60720593427705 3 5.4080222925439285 9.751313878795527 4 2.4046690794811267 5.781209642567144 5 1.2054734460594292 3.622689068572997 6 0.682488237465153 2.4612165638755394 7 0.40829819773130266 1.7178249686578793 8 0.25842449419191543 1.2425877560002652 9 0.17968830629974542 0.9719988085998091 >10 0.6592558283125327 6.6421716009184655 >50 0.03677051713777254 1.5361486687306019 >100 0.01879042152744627 2.1280631120613633 >500 0.0013032845115835603 0.4898399717857096 >1k 7.602492984237435E-4 1.0174101546178131 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3951 0.25804285823448003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2728 0.17816778467822364 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2277 0.14871262672738828 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1879 0.12271893966656237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1799 0.11749407794579338 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.531077150961277E-5 0.0 16-17 0.0 0.0 0.0 1.3062154301922554E-4 0.0 18-19 0.0 0.0 0.0 1.3062154301922554E-4 0.0 20-21 0.0 0.0 0.0 2.285877002836447E-4 0.0 22-23 0.0 0.0 0.0 3.918646290576766E-4 0.0 24-25 0.0 0.0 0.0 5.224861720769022E-4 0.0 26-27 0.0 0.0 0.0 8.490400296249659E-4 0.0 28-29 0.0 0.0 0.0 0.001371526201701868 0.0 30-31 0.0 0.0 0.0 0.003918646290576766 0.0 32-33 0.0 0.0 0.0 0.008784298768042917 0.0 34-35 0.0 0.0 0.0 0.015380686690513807 0.0 36-37 0.0 0.0 0.0 0.026581484004412397 0.0 38-39 0.0 0.0 0.0 0.0455216077422001 0.0 40-41 0.0 0.0 0.0 0.06916410702867992 0.0 42-43 0.0 0.0 0.0 0.09195756628553478 0.0 44-45 0.0 0.0 0.0 0.11994323187740386 0.0 46-47 0.0 0.0 0.0 0.15380686690513806 0.0 48-49 0.0 0.0 0.0 0.19011965586448276 0.0 50-51 0.0 0.0 0.0 0.2306449895861975 0.0 52-53 0.0 0.0 0.0 0.27224795103782085 0.0 54-55 0.0 0.0 0.0 0.31770424800851127 0.0 56-57 0.0 0.0 0.0 0.3719448437472447 0.0 58-59 0.0 0.0 0.0 0.4311490581207087 0.0 60-61 0.0 0.0 0.0 0.49903960510495116 0.0 62-63 0.0 0.0 0.0 0.570587555293732 0.0 64-65 0.0 0.0 0.0 0.6424294039543059 0.0 66-67 0.0 0.0 0.0 0.7221738559675431 0.0 68-69 0.0 0.0 0.0 0.8151437392114769 0.0 70-71 0.0 0.0 0.0 0.9267271923356504 0.0 72-73 0.0 0.0 0.0 1.0395842055042612 0.0 74-75 0.0 0.0 0.0 1.1521473202010788 0.0 76-77 0.0 0.0 0.0 1.2749968814106605 0.0 78-79 0.0 0.0 0.0 1.4196928956902075 0.0 80-81 0.0 0.0 0.0 1.5772224765713934 0.0 82-83 0.0 0.0 0.0 1.7428832485055263 0.0 84-85 0.0 0.0 0.0 1.9136056052316541 0.0 86-87 0.0 0.0 0.0 2.090859039108743 0.0 88-89 0.0 0.0 0.0 2.2905140676136293 0.0 90-91 0.0 0.0 0.0 2.500194299545241 0.0 92-93 0.0 0.0 0.0 2.726920642840862 0.0 94-95 0.0 0.0 0.0 2.9596555771153668 0.0 96-97 0.0 0.0 0.0 3.1956887053511074 0.0 98-99 0.0 0.0 0.0 3.4485393572505734 0.0 100-101 0.0 0.0 0.0 3.730910477872384 0.0 102-103 0.0 0.0 0.0 4.035650537736237 0.0 104-105 0.0 0.0 0.0 4.34479907467699 0.0 106-107 0.0 0.0 0.0 4.671385587610808 0.0 108-109 0.0 0.0 0.0 5.007148263941727 0.0 110-111 0.0 0.0 0.0 5.372464064380746 0.0 112-113 0.0 0.0 0.0 5.770206662874288 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6980 0.0 40.539585 1 ATCAACG 7955 0.0 34.669975 3 TCAACGC 8030 0.0 34.34616 4 TAGGACC 1825 0.0 34.27198 4 CAACGCA 8190 0.0 33.67517 5 AACGCAG 8330 0.0 33.21096 6 TATCAAC 8420 0.0 33.17978 2 GGTATCA 4625 0.0 30.140139 1 CGCAGAG 9390 0.0 29.398548 8 ACGCAGA 9400 0.0 29.367273 7 GCAGAGT 10450 0.0 26.473423 9 GTCCTAC 4590 0.0 25.82745 1 AGTACTT 5345 0.0 25.712109 12-13 GTAGGAC 5330 0.0 25.70475 3 GTACATG 7130 0.0 25.483053 1 GAGTACT 5120 0.0 25.331448 12-13 TACATGG 7315 0.0 24.511219 2 TACAGCG 220 1.5224941E-7 24.368711 5 CTACAGT 4880 0.0 23.924835 4 TCCTACA 5405 0.0 23.805143 2 >>END_MODULE