FastQCFastQC Report
Fri 27 May 2016
SRR939946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358546
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6510.1815666609026457No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3830.10682032430985146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG109.124058E-42981.5110-111
CGCGTGT109.124058E-42981.5110-111
CGTCACG109.124058E-42981.5110-111
CGTCGCA100.00134605122456.756108-109
CCGCGCC600.02047.2968108-109
AGGCGTA150.0020526881987.6665110-111
GTTCGCT150.0020526881987.6665110-111
TTATTCG252.8066352E-61965.4049108-109
CGGCGCC404.5638444E-91842.567108-109
CCGCTCC850.01734.1807108-109
CAGTTCG150.0030282111637.8374108-109
CCGGTCG150.0030282111637.8374108-109
AAGCTCG304.849142E-61637.8374108-109
CGGATCT200.00364882311490.75110-111
CTCCGCC1050.01419.7618110-111
CAGCGCT200.0053827681228.378108-109
CGGCGCA200.0053827681228.378108-109
GGTCGCC200.0053827681228.378108-109
CCGCTCG200.0053827681228.378108-109
CAGCTCG401.149084E-51228.378108-109
Produced by FastQC (version 0.11.5)