##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939946_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 358546 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53136557094487 33.0 31.0 34.0 28.0 34.0 2 31.6772799027182 34.0 31.0 34.0 28.0 34.0 3 31.781511995671405 34.0 31.0 34.0 28.0 34.0 4 34.94144405459829 37.0 35.0 37.0 32.0 37.0 5 34.992650873249175 37.0 35.0 37.0 32.0 37.0 6 35.036932499595586 37.0 36.0 37.0 32.0 37.0 7 34.944598461564205 37.0 36.0 37.0 32.0 37.0 8 34.89069742794509 37.0 36.0 37.0 32.0 37.0 9 36.42746816308089 39.0 38.0 39.0 32.0 39.0 10-11 36.411440930870796 39.0 38.0 39.0 32.0 39.0 12-13 36.33270208006783 39.0 38.0 39.0 32.0 39.0 14-15 37.81415773708255 41.0 39.0 41.0 32.0 41.0 16-17 37.858846563620844 41.0 39.0 41.0 32.0 41.0 18-19 37.92751139323825 41.0 39.0 41.0 32.0 41.0 20-21 38.01761419734149 41.0 39.0 41.0 32.0 41.0 22-23 38.0577303888483 41.0 39.0 41.0 32.0 41.0 24-25 38.12096634741428 41.0 39.0 41.0 32.0 41.0 26-27 38.12602427582514 41.0 39.0 41.0 32.0 41.0 28-29 38.08930932153754 41.0 39.0 41.0 32.0 41.0 30-31 38.024812715802156 40.5 38.0 41.0 32.0 41.0 32-33 37.92273515811081 40.0 38.0 41.0 32.0 41.0 34-35 37.85252240995577 40.0 38.0 41.0 32.0 41.0 36-37 38.02892097527235 41.0 38.0 41.0 33.0 41.0 38-39 37.98319044139385 41.0 38.0 41.0 32.0 41.0 40-41 37.91754196114306 41.0 38.0 41.0 32.0 41.0 42-43 37.841981224166496 41.0 38.0 41.0 32.0 41.0 44-45 37.757676839234016 41.0 38.0 41.0 32.0 41.0 46-47 37.67081490241141 40.0 38.0 41.0 31.0 41.0 48-49 37.57732898986462 40.0 38.0 41.0 31.0 41.0 50-51 37.47044730662174 40.0 38.0 41.0 31.0 41.0 52-53 37.37064002945229 40.0 38.0 41.0 30.5 41.0 54-55 37.234151266504156 40.0 37.0 41.0 30.0 41.0 56-57 37.04739001411255 40.0 37.0 41.0 30.0 41.0 58-59 36.88781913617778 40.0 37.0 41.0 30.5 41.0 60-61 36.68061559744078 40.0 36.0 41.0 30.0 41.0 62-63 36.440070451211284 40.0 36.0 41.0 30.0 41.0 64-65 36.151976315451854 39.0 35.0 41.0 30.0 41.0 66-67 35.84782984610064 39.0 35.0 41.0 29.5 41.0 68-69 35.476177394253455 38.5 35.0 40.5 29.0 41.0 70-71 35.07780591611676 37.5 35.0 40.0 29.0 41.0 72-73 34.687550551393684 37.0 34.5 39.5 28.5 41.0 74-75 34.26567860190882 36.5 34.0 39.0 28.0 41.0 76-77 33.80915558951989 36.0 34.0 39.0 27.5 40.5 78-79 33.34095206751714 36.0 34.0 38.5 26.0 40.0 80-81 32.87582346477161 35.0 34.0 37.0 26.0 39.0 82-83 32.6181480200588 35.0 34.0 37.0 26.0 39.0 84-85 32.15879691866594 35.0 34.0 36.5 24.5 38.5 86-87 31.469450223960106 35.0 34.0 36.0 2.0 37.0 88-89 16.06608635990919 18.0 16.5 18.5 2.0 19.5 90-91 2.782726623641039 2.0 2.0 2.0 2.0 2.0 92-93 2.30200030121658 2.0 2.0 2.0 2.0 2.0 94-95 2.1006760638802273 2.0 2.0 2.0 2.0 2.0 96-97 2.0000850657935105 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.36713276136989137 1101 2 -0.18006042038496517 1101 3 -0.20752530807521197 1101 4 -0.32530861245987097 1101 5 -0.22019403822693562 1101 6 -0.18895971991714333 1101 7 -0.23873852922814365 1101 8 -0.0652919474793876 1101 9 0.0022367590546252814 1101 10-11 -0.20592833033131086 1101 12-13 -0.27819305927747706 1101 14-15 -0.2568252736403167 1101 16-17 -0.31642261756114465 1101 18-19 -0.3706265931513997 1101 20-21 -0.35544845907658384 1101 22-23 -0.3898154731349024 1101 24-25 -0.42462115633716735 1101 26-27 -0.22065319628223534 1101 28-29 -0.054587559068934866 1101 30-31 -0.13795735468298886 1101 32-33 -0.04892202307972582 1101 34-35 -0.03067590725932945 1101 36-37 -0.031725184548662355 1101 38-39 -0.09354957872668734 1101 40-41 -0.007103607174414606 1101 42-43 -0.02015916222489267 1101 44-45 -0.20246616529928474 1101 46-47 -0.09625111694533217 1101 48-49 -0.006697896437202644 1101 50-51 -0.08623996100376274 1101 52-53 0.011978562755317057 1101 54-55 0.024765926652619896 1101 56-57 -0.10280178031771925 1101 58-59 -0.1321756327595125 1101 60-61 -0.3896999503778602 1101 62-63 -0.3370630303170117 1101 64-65 -0.2435168287926217 1101 66-67 -0.06156749767695402 1101 68-69 -0.24973531657536086 1101 70-71 -0.1517223730742785 1101 72-73 -0.29180090984466744 1101 74-75 -0.13721633237652497 1101 76-77 -0.11626395924683663 1101 78-79 -0.13476040863424998 1101 80-81 -0.11467527150726653 1101 82-83 -0.19607400912488515 1101 84-85 -0.1910711409666419 1101 86-87 -0.2118315533040125 1101 88-89 0.3429319374980544 1101 90-91 -0.06523869885251532 1101 92-93 -0.152085541523316 1101 94-95 0.014799802356590508 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.35495379019758033 1102 2 0.47211472727519066 1102 3 0.3843241759255669 1102 4 0.5791220034939144 1102 5 0.4648111542180544 1102 6 0.4719452258867136 1102 7 0.27271324593899493 1102 8 0.5250484821954373 1102 9 0.8175885472028668 1102 10-11 0.7500819247475548 1102 12-13 0.6875753414494667 1102 14-15 0.6434432731241486 1102 16-17 0.6422548504728667 1102 18-19 0.525554931480265 1102 20-21 0.6175751665481357 1102 22-23 0.6316337869416913 1102 24-25 0.5676307421505342 1102 26-27 0.5855367660725292 1102 28-29 0.3486463605779804 1102 30-31 0.3345632528330711 1102 32-33 0.3681023002797872 1102 34-35 0.4567912342997076 1102 36-37 0.31802655167513194 1102 38-39 0.24691863377412204 1102 40-41 0.21040304564572665 1102 42-43 0.19113683959246686 1102 44-45 0.193731585722972 1102 46-47 0.1900939697035895 1102 48-49 0.24841138830350218 1102 50-51 0.38624616543227575 1102 52-53 0.41793040282801286 1102 54-55 0.3871722192468994 1102 56-57 0.2599675777662682 1102 58-59 0.4710891170295497 1102 60-61 0.3164948798941012 1102 62-63 0.23367794780980944 1102 64-65 0.40345573238087695 1102 66-67 0.5508996438820759 1102 68-69 0.3051666760254008 1102 70-71 0.2714882617021246 1102 72-73 0.5769052789007105 1102 74-75 0.6019464670003742 1102 76-77 0.6855205380595777 1102 78-79 0.3908184678504725 1102 80-81 0.23258368546035513 1102 82-83 0.08093887462924698 1102 84-85 0.23621231785077867 1102 86-87 0.4125660419448991 1102 88-89 1.249954719350459 1102 90-91 -0.020522822951950737 1102 92-93 -0.029797377052620977 1102 94-95 0.08056130686752727 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.21621891116532055 1103 2 0.32739446517841486 1103 3 0.3738025025384708 1103 4 0.10249649946165817 1103 5 0.33754300905676615 1103 6 0.2990621613705855 1103 7 0.17493098787447536 1103 8 0.13366743380834123 1103 9 0.1775935875254504 1103 10-11 0.31220390055400316 1103 12-13 0.34911768015914646 1103 14-15 0.3515770936886611 1103 16-17 0.2703735500696425 1103 18-19 0.24833718954477746 1103 20-21 0.4467082310642638 1103 22-23 0.4889296538771717 1103 24-25 0.30515594376343813 1103 26-27 0.4120551632499527 1103 28-29 0.37595860856185226 1103 30-31 0.3677978798491992 1103 32-33 0.5210760906023637 1103 34-35 0.49519219196099584 1103 36-37 0.4884839609493241 1103 38-39 0.44046702087089784 1103 40-41 0.37229190653282274 1103 42-43 0.33718018636665903 1103 44-45 0.29809776515845954 1103 46-47 0.2994374676874614 1103 48-49 0.49913593467447015 1103 50-51 0.47275070172259603 1103 52-53 0.40302994919898083 1103 54-55 0.3806197998920595 1103 56-57 0.4176981725243323 1103 58-59 0.32819597186825433 1103 60-61 0.3894220472328058 1103 62-63 0.3390836937775532 1103 64-65 0.3209834541550691 1103 66-67 0.35687872654336417 1103 68-69 0.14192322844476024 1103 70-71 0.3293574653311566 1103 72-73 0.2615700974490949 1103 74-75 0.2810039266777977 1103 76-77 0.1038988142692574 1103 78-79 0.11464029244724117 1103 80-81 0.09703051005712382 1103 82-83 0.08424658632279858 1103 84-85 -0.06604952690728538 1103 86-87 0.38090651573522294 1103 88-89 0.9674446387052988 1103 90-91 -0.5939225205325958 1103 92-93 -0.30701511898810496 1103 94-95 -0.10290643506795671 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.11018494419866443 1104 2 0.08052994171098149 1104 3 0.05696970799824186 1104 4 0.2916652614348365 1104 5 0.13983587304144152 1104 6 0.22824080121733203 1104 7 0.1635564668016798 1104 8 -0.056137403356409266 1104 9 0.013037193847289075 1104 10-11 0.16662499729729774 1104 12-13 0.17194454797523662 1104 14-15 0.0049946882767812895 1104 16-17 0.09047277779952623 1104 18-19 0.019888913682713394 1104 20-21 0.23268763719453034 1104 22-23 0.15525957485418473 1104 24-25 0.1282073087059672 1104 26-27 0.23671168264650788 1104 28-29 0.24981741483579611 1104 30-31 0.28875618324958197 1104 32-33 0.23057680899317035 1104 34-35 0.3695299254380018 1104 36-37 0.15520675189759459 1104 38-39 0.09168828523871753 1104 40-41 0.25710617257688284 1104 42-43 0.2584415608877322 1104 44-45 0.3064378334055817 1104 46-47 0.2951682806663882 1104 48-49 0.31541871125493515 1104 50-51 0.18803596273792067 1104 52-53 0.1953064997258025 1104 54-55 0.19365859299550436 1104 56-57 0.15516749125038842 1104 58-59 0.10444716918625829 1104 60-61 0.2849822423956425 1104 62-63 0.028476952877170447 1104 64-65 -0.02696464210929861 1104 66-67 0.24668431203186714 1104 68-69 0.2554663917589366 1104 70-71 -0.09101714554049067 1104 72-73 0.056940966701972684 1104 74-75 -0.008133405697677176 1104 76-77 -0.042753933575568226 1104 78-79 -0.08337739792631993 1104 80-81 -0.3627807626919193 1104 82-83 -0.0954222055833327 1104 84-85 -0.009682040077784393 1104 86-87 -0.00211925653489331 1104 88-89 0.4902834797014677 1104 90-91 -0.6590641033870019 1104 92-93 -0.30701511898810496 1104 94-95 -0.10290643506795671 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.29079210350936435 1105 2 0.17282437420487895 1105 3 0.2975124800904574 1105 4 0.39657657389454926 1105 5 0.18736283419854516 1105 6 0.3519036074953519 1105 7 0.40443240564385263 1105 8 0.24305720223934202 1105 9 0.15485758799803762 1105 10-11 -0.012723734134091558 1105 12-13 0.09103934840111805 1105 14-15 0.17689424574442825 1105 16-17 0.29864586516888636 1105 18-19 0.35018028973381377 1105 20-21 0.19969145412665057 1105 22-23 0.14715493152367287 1105 24-25 0.48743530340426844 1105 26-27 0.4081802961658312 1105 28-29 0.2438457629805626 1105 30-31 0.33492150638511475 1105 32-33 0.31400791952485463 1105 34-35 0.2640717849430416 1105 36-37 0.21920192822248197 1105 38-39 0.23081437436806596 1105 40-41 0.24759281331448335 1105 42-43 0.3830730857050284 1105 44-45 0.2257933730270807 1105 46-47 0.41439308855229484 1105 48-49 0.37592106877182374 1105 50-51 0.14003641178876336 1105 52-53 0.1797146579655191 1105 54-55 0.0888251401567075 1105 56-57 0.115770523067809 1105 58-59 0.09841395390984076 1105 60-61 0.0739591307630505 1105 62-63 0.1907237280403109 1105 64-65 0.14164670439136273 1105 66-67 0.2889457899885386 1105 68-69 0.4063999829343601 1105 70-71 0.2288084489086657 1105 72-73 0.23635669362111855 1105 74-75 0.19172137705587033 1105 76-77 0.4169618607012069 1105 78-79 0.4418219589169965 1105 80-81 0.2554680838992809 1105 82-83 0.3346471800137252 1105 84-85 0.4935355540237154 1105 86-87 0.3771313355603674 1105 88-89 0.39063402906446854 1105 90-91 -0.7443131455325958 1105 92-93 -0.30701511898810496 1105 94-95 -0.10290643506795671 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.06326084070733984 1106 2 0.024586939429351418 1106 3 -0.08883940991471206 1106 4 0.20257403925566564 1106 5 0.06030167010545284 1106 6 0.1351459628687124 1106 7 -0.01619728927908426 1106 8 0.1745440723851175 1106 9 0.12520302104604752 1106 10-11 0.13264807190231664 1106 12-13 0.18944422510296732 1106 14-15 0.15660474467181018 1106 16-17 0.12899143981683636 1106 18-19 -0.048011125061968585 1106 20-21 0.04355982282456239 1106 22-23 -0.17565221644117912 1106 24-25 -0.10497104593693507 1106 26-27 -0.30997564784555465 1106 28-29 -0.05580271517410296 1106 30-31 0.07328199150650505 1106 32-33 -0.043735473068046815 1106 34-35 -0.09059534900342214 1106 36-37 0.13186308665157043 1106 38-39 0.07447919315553975 1106 40-41 0.18075764272195727 1106 42-43 -0.25275134453222137 1106 44-45 -0.047075016910824274 1106 46-47 -0.06068001241553844 1106 48-49 0.16508938153776853 1106 50-51 -0.022692006610732562 1106 52-53 -0.10043373876918338 1106 54-55 0.1542036013901864 1106 56-57 -0.19089777199813796 1106 58-59 -0.26815819479841707 1106 60-61 -0.05559419223348527 1106 62-63 -0.1549252598741333 1106 64-65 -0.10351765773631882 1106 66-67 -0.07279780084427756 1106 68-69 -0.11501877764138158 1106 70-71 -0.10071407610142558 1106 72-73 -0.16632520919884541 1106 74-75 0.014252405142215707 1106 76-77 0.3381384853834888 1106 78-79 0.09010187367383082 1106 80-81 -0.08451867650654776 1106 82-83 0.19486149151942556 1106 84-85 0.23026586986237163 1106 86-87 0.2470735627857863 1106 88-89 0.5130029252221533 1106 90-91 -0.23775883841649081 1106 92-93 -0.2807978905236852 1106 94-95 -0.10290643506795671 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.13758914105279274 1107 2 -0.15985787423932862 1107 3 -0.03658841127853307 1107 4 -0.05228414402262871 1107 5 -0.3154866813314072 1107 6 -0.28616773696582953 1107 7 -0.1979317662844906 1107 8 -0.17913582404747785 1107 9 -0.2551337114579155 1107 10-11 -0.21788361099867615 1107 12-13 -0.2956229621698725 1107 14-15 -0.38337012500357304 1107 16-17 -0.42118613396455373 1107 18-19 -0.0485666921557808 1107 20-21 -0.20159930128323822 1107 22-23 -0.2523083602170999 1107 24-25 -0.29967740882436544 1107 26-27 -0.27479319338129216 1107 28-29 -0.07986863554628343 1107 30-31 -0.09690326675431749 1107 32-33 -0.283004893685991 1107 34-35 -0.17944281554824215 1107 36-37 -0.32928875450816264 1107 38-39 -0.44637447635646055 1107 40-41 -0.353434829257381 1107 42-43 -0.2601294832266845 1107 44-45 -0.29788154110309506 1107 46-47 -0.19365439056115008 1107 48-49 -0.2479271574003974 1107 50-51 -0.18150285072656658 1107 52-53 -0.13311008372615873 1107 54-55 -0.2481637028805821 1107 56-57 -0.13437011825663348 1107 58-59 0.04869331475180161 1107 60-61 -0.08377753975887714 1107 62-63 -0.029948624845367533 1107 64-65 -0.16501253128855353 1107 66-67 -0.2870442026387039 1107 68-69 -0.15210331383804743 1107 70-71 -0.35809740701172643 1107 72-73 -0.299460546035192 1107 74-75 -0.25826796565122834 1107 76-77 -0.5925021648203881 1107 78-79 -0.344064291078638 1107 80-81 -0.2044709773504536 1107 82-83 -0.13420557114023524 1107 84-85 -0.09039499536847018 1107 86-87 0.1775342161742266 1107 88-89 0.5797800130121402 1107 90-91 -0.1583612046823184 1107 92-93 -0.30701511898810496 1107 94-95 -0.10290643506795671 1107 96-97 0.0 1107 98-99 0.0 1107 100-101 0.0 1107 102-103 0.0 1107 104-105 0.0 1107 106-107 0.0 1107 108-109 0.0 1107 110-111 0.0 1107 112-113 0.0 1107 114-115 0.0 1107 116-117 0.0 1107 118-119 0.0 1107 120-121 0.0 1107 122-123 0.0 1107 124-125 0.0 1108 1 0.12901679785596798 1108 2 0.34106634017841486 1108 3 0.2944831455240795 1108 4 0.010559111871728533 1108 5 0.13953266733014402 1108 6 0.16405766496770724 1108 7 0.16211623967303979 1108 8 0.31470385467164874 1108 9 0.3251599094678994 1108 10-11 0.1669419850863747 1108 12-13 0.2951046405908002 1108 14-15 0.10386042327499467 1108 16-17 0.11840215843294999 1108 18-19 0.11524366436491817 1108 20-21 0.13364618070455236 1108 22-23 0.17458893894911398 1108 24-25 0.24667460383538042 1108 26-27 0.1438377059657867 1108 28-29 0.15541707304026886 1108 30-31 0.2885839072772569 1108 32-33 0.1447833747750238 1108 34-35 -0.17049322845267056 1108 36-37 -0.05534894147873359 1108 38-39 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12-13 0.21346213504636324 2216 14-15 0.15884771163978684 2216 16-17 0.1006058691485876 2216 18-19 0.08950260307861413 2216 20-21 -0.06163293434926942 2216 22-23 0.07962220556890287 2216 24-25 -0.0499838588681385 2216 26-27 -0.0833557272575618 2216 28-29 -0.020265839276497388 2216 30-31 -0.2164493593425263 2216 32-33 -0.3087758830818501 2216 34-35 -0.22415255662922817 2216 36-37 -0.1800149229792467 2216 38-39 -0.14623410694865413 2216 40-41 -0.18965152626793014 2216 42-43 -0.29515651476116034 2216 44-45 -0.4150469129430405 2216 46-47 -0.406120861729832 2216 48-49 -0.3769933533894374 2216 50-51 -0.4190579213977017 2216 52-53 -0.40705140653410865 2216 54-55 -0.4535672301831255 2216 56-57 -0.5439221869681532 2216 58-59 -0.4680328815151995 2216 60-61 -0.5608320058686971 2216 62-63 -0.7103024669367386 2216 64-65 -0.7569447648298961 2216 66-67 -0.7798561771220491 2216 68-69 -0.726236428510127 2216 70-71 -0.9100618838229764 2216 72-73 -0.9151833095057924 2216 74-75 -1.0536941466304768 2216 76-77 -0.9404723675916458 2216 78-79 -0.8564696112181736 2216 80-81 -0.7536141680443791 2216 82-83 -0.3989756293726927 2216 84-85 -0.3920877670388663 2216 86-87 -0.5701816539076354 2216 88-89 -1.2080370342270328 2216 90-91 -0.7443131455325958 2216 92-93 -0.30701511898810496 2216 94-95 -0.10290643506795671 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4808.0 3 878.0 4 567.0 5 495.0 6 483.0 7 512.0 8 533.0 9 723.0 10 884.0 11 1016.0 12 1282.0 13 1543.0 14 1675.0 15 1900.0 16 2284.0 17 2730.0 18 3506.0 19 4303.0 20 3861.0 21 5767.0 22 6428.0 23 7238.0 24 10962.0 25 18460.0 26 33045.0 27 92689.0 28 144253.0 29 5495.0 30 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.334038031382306 20.273549279590345 15.145058095753406 22.247354593273947 2 13.57804664446564 20.927534291658997 44.16776835070927 21.3266507131661 3 18.876547446660695 28.25148641371825 28.930312237558347 23.941653902062704 4 11.469564052769035 18.282495144980913 39.88760909841738 30.360331703832678 5 12.248422803155231 39.24925151162564 33.835580891154585 14.66674479406455 6 27.578609160330892 37.110719405599276 21.798318765235145 13.512352668834682 7 27.66730070897458 32.7112839077831 22.523190887640638 17.09822449560168 8 25.090783330451323 37.1578542223313 21.133411054648494 16.617951392568877 9 26.343621181103682 19.22235919519392 21.946416917215643 32.487602706486754 10-11 23.65076022641541 27.99164399398122 29.250344099546915 19.10725168005645 12-13 25.645291522970272 25.604570765564183 28.91996558259271 19.83017212887283 14-15 21.243159873489038 28.98805174231479 26.61011418339627 23.158674200799897 16-17 20.19297411346677 31.055054379430224 28.825351315244507 19.926620191858497 18-19 20.850748704045387 29.35572542079301 30.158052769793553 19.63547310536805 20-21 22.529672380107687 28.684207223451747 28.44852952455859 20.33759087188198 22-23 21.18531678685132 30.207323756678022 28.46403036713611 20.143329089334546 24-25 21.179569706536956 29.398598785093128 29.635806842078843 19.786024666291073 26-27 20.00998477182844 30.218158897324194 29.181611285581212 20.59024504526616 28-29 19.84015864205967 30.57764340678803 28.109320221005568 21.472877730146735 30-31 20.800399390873135 29.270581738465918 29.13126907007748 20.797749800583468 32-33 20.090170369843545 29.864437648430386 29.71982479113413 20.325567190591936 34-35 20.397661666843305 30.2869087927351 29.404455774154503 19.91097376626709 36-37 20.077479598154767 29.72449839072253 29.24046008043598 20.95756193068672 38-39 20.62399245842932 30.39163733523732 28.57108990199305 20.413280304340308 40-41 21.928985402152026 28.936872814088012 29.351882324722627 19.782259459037334 42-43 20.019746420264067 30.1731158623998 29.377820419137297 20.42931729819884 44-45 20.05223876434265 30.237542742074936 28.87732675863069 20.832891734951723 46-47 21.043882793281753 29.350906159879063 28.657410764588086 20.94780028225109 48-49 20.485098146402414 29.568172563632 28.968249541202525 20.97847974876306 50-51 19.757018625225214 30.568462624042663 29.077858907922554 20.59665984280957 52-53 19.86732525254779 30.054023751485165 29.329151629079668 20.749499366887374 54-55 19.979137555468164 29.498185692490914 29.342414632717777 21.180262119323142 56-57 20.586619290132926 29.857675165808573 28.04410033858975 21.511605205468754 58-59 20.73164949546223 30.170605724230644 27.877176150340542 21.22056862996659 60-61 20.189320198802942 29.140054553669543 29.863532154869947 20.807093092657567 62-63 20.54004228188294 29.075348769753397 29.59843367378244 20.786175274581225 64-65 20.741411143897853 29.73328387431459 28.396356394995344 21.12894858679221 66-67 20.567095993261674 30.617689222582317 27.869785187953568 20.945429596202437 68-69 21.187298665439485 29.152407647575618 29.242493968678968 20.417799718305933 70-71 21.596113190497174 29.01482654945251 28.72281380910678 20.666246450943532 72-73 20.857295856040786 29.778605813480002 28.627846915040188 20.73625141543902 74-75 20.338394515627005 30.268222208586902 29.203086912139586 20.190296363646507 76-77 20.53055953768833 29.31729819883641 29.475297451373045 20.676844812102214 78-79 20.518316364310806 29.083756454899028 29.163523179066537 21.234404001723632 80-81 20.6984598907811 29.78362608981832 28.949005148572287 20.568908870828288 82-83 20.518316364310806 29.65188518612003 29.60991003931161 20.219888410257557 84-85 20.445075387816345 29.645568490514467 29.371126717352862 20.538229404316322 86-87 19.653121958158202 30.607936051943852 28.43231318036152 21.306628809536427 88-89 29.868502902958255 18.78715095382914 35.00501105888858 16.339335084324023 90-91 30.54373200455538 15.73213319561261 17.688410649898035 36.035724149933976 92-93 22.252555734905375 18.05647179919059 36.447469997894586 23.24350246800945 94-95 16.07908425565456 7.23061104360194 28.57128420967534 48.11902049106816 96-97 27.980098481739844 7.385860527395634 15.669881001231023 48.9641599896335 98-99 57.257533097705625 13.99189280936121 18.846222967711014 9.90435112522215 100-101 30.997191213539793 7.183003240907454 12.967951026287361 48.851854519265395 102-103 23.56012080285793 2.1170196022533236 37.539458406459445 36.783401188429295 104-105 41.84843827163919 4.532313821670542 41.35292452987232 12.266323376817947 106-107 6.097570267202036 2.0442060510937226 7.029386890566785 84.82883679113745 108-109 18.672242200687318 11.676393537826307 22.513425634905694 47.137938626580684 110-111 40.626963560896186 2.752081087898732 26.023803517783843 30.597151833421236 112-113 27.765263919689126 2.852965881248492 39.34337606775503 30.03839413130735 114-115 29.64840607933618 4.045129334067143 24.33427881969434 41.97218576690233 116-117 20.34655464563913 4.31569612769731 20.993534366113916 54.34421486054964 118-119 21.218765001954896 4.467056325690275 16.43001727235384 57.88416140000099 120-121 26.031344037447802 4.376498374463787 30.671058341455087 38.921099246633325 122-123 34.672645636523974 1.774094076554638 34.50837766647262 29.04488262044877 124-125 38.87285050834693 2.1653208373429282 20.423134317950435 38.53869433635971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 21.5 2 55.0 3 89.5 4 103.0 5 98.5 6 91.0 7 74.5 8 62.5 9 56.5 10 57.0 11 59.5 12 55.5 13 60.0 14 62.0 15 74.5 16 106.0 17 133.5 18 183.0 19 261.0 20 373.0 21 536.5 22 817.0 23 1195.5 24 1616.5 25 2199.5 26 2905.5 27 3628.5 28 4559.0 29 5879.5 30 7440.0 31 9133.5 32 11205.5 33 13414.0 34 15274.5 35 16587.0 36 17279.0 37 17203.5 38 16776.5 39 16273.0 40 15615.0 41 14783.5 42 13972.5 43 13555.5 44 13148.0 45 12551.5 46 11909.5 47 11408.5 48 10771.0 49 10070.5 50 9490.5 51 8715.0 52 7898.5 53 6990.5 54 6003.0 55 5132.0 56 4304.5 57 3565.0 58 2922.0 59 2365.0 60 1863.5 61 1415.5 62 1073.0 63 828.0 64 631.5 65 441.5 66 310.5 67 217.5 68 149.0 69 124.0 70 88.5 71 54.0 72 41.5 73 39.5 74 32.0 75 20.0 76 10.5 77 6.0 78 3.5 79 3.0 80 2.5 81 0.5 82 0.5 83 1.0 84 1.5 85 2.5 86 1.5 87 0.5 88 0.5 89 1.0 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0011156169640715557 3 0.03709426405537923 4 0.04295125311675489 5 0.043787965839808554 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.183563615268334E-4 12-13 0.001812877566616278 14-15 0.0 16-17 1.3945212050894446E-4 18-19 0.007390962386974056 20-21 0.0015339733255983889 22-23 1.3945212050894446E-4 24-25 0.0 26-27 0.0 28-29 8.367127230536668E-4 30-31 0.0 32-33 6.972606025447223E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0019523296871252224 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.789042410178889E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.3945212050894446E-4 80-81 0.0 82-83 1.3945212050894446E-4 84-85 0.0 86-87 0.0013945212050894447 88-89 19.296268819063663 90-91 26.284772386248907 92-93 40.38840204604151 94-95 44.800527686824005 96-97 48.34358771259476 98-99 93.01637725703257 100-101 51.59268266833266 102-103 53.039916774974486 104-105 74.86835719823955 106-107 81.69704863532155 108-109 12.593363194680737 110-111 39.18911380966459 112-113 52.019685061331046 114-115 86.8240058458329 116-117 66.50457681859511 118-119 71.82272288632421 120-121 80.39735487217818 122-123 75.80547544805967 124-125 50.00432301573577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 358546.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.7468971218837 #Duplication Level Percentage of deduplicated Percentage of total 1 92.84541099582614 79.61205904898114 2 5.230243659887982 8.969543300535987 3 0.894525890020519 2.301084583933527 4 0.34113763555220905 1.1700597496039171 5 0.18095681177813777 0.7758242561522027 6 0.10708182842826884 0.5509160715517182 7 0.0773237475637851 0.46411899992012706 8 0.053692549253494704 0.3683175597640867 9 0.037885656150939015 0.2923719714332665 >10 0.21058150516689778 3.3106322276691955 >50 0.012370298069746497 0.7215651148894193 >100 0.008463888142519398 1.281789943672751 >500 3.2553415932766916E-4 0.1817171718926308 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 651 0.1815666609026457 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 383 0.10682032430985146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.789042410178889E-4 0.0 4 0.0 0.0 0.0 2.789042410178889E-4 0.0 5 0.0 0.0 0.0 2.789042410178889E-4 0.0 6 0.0 0.0 0.0 2.789042410178889E-4 0.0 7 0.0 0.0 0.0 2.789042410178889E-4 0.0 8 0.0 0.0 0.0 2.789042410178889E-4 0.0 9 0.0 0.0 0.0 2.789042410178889E-4 0.0 10-11 0.0 0.0 0.0 2.789042410178889E-4 0.0 12-13 0.0 0.0 0.0 2.789042410178889E-4 0.0 14-15 0.0 0.0 0.0 2.789042410178889E-4 0.0 16-17 0.0 0.0 0.0 2.789042410178889E-4 0.0 18-19 0.0 0.0 0.0 2.789042410178889E-4 0.0 20-21 0.0 0.0 0.0 2.789042410178889E-4 0.0 22-23 0.0 0.0 0.0 8.367127230536667E-4 0.0 24-25 0.0 0.0 0.0 0.0013945212050894447 0.0 26-27 0.0 0.0 0.0 0.0013945212050894447 0.0 28-29 0.0 0.0 0.0 0.0018128775666162778 0.0 30-31 0.0 0.0 0.0 0.0029284945306878338 0.0 32-33 0.0 0.0 0.0 0.006135893302393556 0.0 34-35 1.3945212050894446E-4 0.0 0.0 0.010458909038170833 0.0 36-37 2.789042410178889E-4 0.0 0.0 0.016176445979037557 0.0 38-39 2.789042410178889E-4 0.0 0.0 0.03249234407858406 0.0 40-41 2.789042410178889E-4 0.0 0.0 0.05661756092663145 0.0 42-43 2.789042410178889E-4 0.0 0.0 0.07753537900297311 0.0 44-45 2.789042410178889E-4 0.0 0.0 0.10110278736898473 0.0 46-47 2.789042410178889E-4 0.0 0.0 0.12871430722975574 0.0 48-49 2.789042410178889E-4 0.0 0.0 0.16274062463393818 0.0 50-51 2.789042410178889E-4 0.0 0.0 0.20457626078662153 0.0 52-53 2.789042410178889E-4 0.0 0.0 0.24850367874693902 0.0 54-55 2.789042410178889E-4 0.0 0.0 0.29326780943031017 0.0 56-57 2.789042410178889E-4 0.0 0.0 0.34584125886218225 0.0 58-59 2.789042410178889E-4 0.0 0.0 0.4041322452349211 0.0 60-61 2.789042410178889E-4 0.0 0.0 0.4706509067176875 0.0 62-63 2.789042410178889E-4 0.0 0.0 0.5394008021285972 0.0 64-65 2.789042410178889E-4 0.0 0.0 0.6060589157318725 0.0 66-67 2.789042410178889E-4 0.0 0.0 0.6916825177243645 0.0 68-69 2.789042410178889E-4 0.0 0.0 0.7870677681524825 0.0 70-71 2.789042410178889E-4 0.0 0.0 0.8951431615469145 0.0 72-73 2.789042410178889E-4 0.0 0.0 1.0100517088462846 0.0 74-75 2.789042410178889E-4 0.0 0.0 1.119939979807333 0.0 76-77 2.789042410178889E-4 0.0 0.0 1.2447496276628383 0.0 78-79 2.789042410178889E-4 0.0 0.0 1.2798915620310922 0.0 80-81 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 82-83 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 84-85 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 86-87 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 88-89 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 90-91 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 92-93 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 94-95 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 96-97 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 98-99 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 100-101 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 102-103 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 104-105 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 106-107 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 108-109 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 110-111 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 112-113 2.789042410178889E-4 0.0 0.0 1.2810071789951638 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTCG 10 9.124058E-4 2981.5 110-111 CGCGTGT 10 9.124058E-4 2981.5 110-111 CGTCACG 10 9.124058E-4 2981.5 110-111 CGTCGCA 10 0.0013460512 2456.756 108-109 CCGCGCC 60 0.0 2047.2968 108-109 AGGCGTA 15 0.002052688 1987.6665 110-111 GTTCGCT 15 0.002052688 1987.6665 110-111 TTATTCG 25 2.8066352E-6 1965.4049 108-109 CGGCGCC 40 4.5638444E-9 1842.567 108-109 CCGCTCC 85 0.0 1734.1807 108-109 CAGTTCG 15 0.003028211 1637.8374 108-109 CCGGTCG 15 0.003028211 1637.8374 108-109 AAGCTCG 30 4.849142E-6 1637.8374 108-109 CGGATCT 20 0.0036488231 1490.75 110-111 CTCCGCC 105 0.0 1419.7618 110-111 CAGCGCT 20 0.005382768 1228.378 108-109 CGGCGCA 20 0.005382768 1228.378 108-109 GGTCGCC 20 0.005382768 1228.378 108-109 CCGCTCG 20 0.005382768 1228.378 108-109 CAGCTCG 40 1.149084E-5 1228.378 108-109 >>END_MODULE