FastQCFastQC Report
Fri 27 May 2016
SRR939946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358546
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.48250433696094785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.32603905774991215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7650.21336174437868502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.20778365955832723No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.11211950488919135No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG250.002048091371.4666752
AGTCGAT150.004236629759.4974270-71
TACCTGG5800.049.2873652
GTACCTG5950.049.0594561
ACCTGGG6400.045.597233
GTACACG705.4056163E-542.5515671
ACACGGG801.1892726E-437.222233
CCTGGGG5250.036.3005334
TAGGACC4550.035.3406644
GTATCAA36150.034.276671
GAGTACT21550.029.67968812-13
ATCAACG42150.029.5305143
TATCAAC42100.029.4241242
TCAACGC42400.029.3563964
CAACGCA42750.029.1160555
GTACATG28000.028.5095481
AACGCAG43650.028.4878836
TACATGG27700.028.3802682
GGTATCA25800.028.1697961
ACATGGG27050.027.7412243
Produced by FastQC (version 0.11.5)