##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939946_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 358546 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81074394917249 34.0 31.0 34.0 31.0 34.0 2 32.86034985747993 34.0 31.0 34.0 31.0 34.0 3 32.90272098977537 34.0 31.0 34.0 31.0 34.0 4 36.3317621727756 37.0 37.0 37.0 35.0 37.0 5 36.24172909473261 37.0 37.0 37.0 35.0 37.0 6 36.384985469089045 37.0 37.0 37.0 35.0 37.0 7 36.381554946924524 37.0 37.0 37.0 35.0 37.0 8 36.404483664578606 37.0 37.0 37.0 35.0 37.0 9 38.235333820486076 39.0 39.0 39.0 37.0 39.0 10-11 38.22432965365671 39.0 39.0 39.0 37.0 39.0 12-13 38.207550774517074 39.0 39.0 39.0 37.0 39.0 14-15 39.67616986383895 41.0 40.0 41.0 37.0 41.0 16-17 39.698328805787824 41.0 40.0 41.0 37.0 41.0 18-19 39.787539116319806 41.0 40.0 41.0 37.5 41.0 20-21 39.81764125105286 41.0 40.0 41.0 37.5 41.0 22-23 39.75321994946255 41.0 40.0 41.0 37.0 41.0 24-25 39.71309399630731 41.0 40.0 41.0 37.0 41.0 26-27 39.614777462306094 41.0 40.0 41.0 37.0 41.0 28-29 39.52352138916624 41.0 40.0 41.0 36.0 41.0 30-31 39.35307324583178 41.0 39.0 41.0 36.0 41.0 32-33 39.125820675729194 41.0 39.0 41.0 35.0 41.0 34-35 38.978906472251815 41.0 39.0 41.0 35.0 41.0 36-37 38.928025971562924 41.0 39.0 41.0 35.0 41.0 38-39 38.961505636654714 41.0 39.0 41.0 35.0 41.0 40-41 38.859761369531384 41.0 39.0 41.0 35.0 41.0 42-43 38.71280811946026 41.0 39.0 41.0 35.0 41.0 44-45 38.47901524490581 41.0 39.0 41.0 35.0 41.0 46-47 38.130668031438084 41.0 39.0 41.0 34.5 41.0 48-49 37.76202077278787 41.0 39.0 41.0 33.5 41.0 50-51 37.55391218978876 41.0 39.0 41.0 33.0 41.0 52-53 37.44268796751324 41.0 38.5 41.0 33.0 41.0 54-55 37.346075538424635 41.0 38.0 41.0 33.0 41.0 56-57 37.205005773317794 40.5 38.0 41.0 33.0 41.0 58-59 37.04357320957423 40.0 38.0 41.0 32.0 41.0 60-61 36.86106664137935 40.0 37.0 41.0 32.0 41.0 62-63 36.66585180144249 40.0 37.0 41.0 31.0 41.0 64-65 36.44128786822333 39.5 36.0 41.0 31.0 41.0 66-67 36.18261255180646 39.0 36.0 41.0 31.0 41.0 68-69 35.866756287896116 39.0 35.0 41.0 31.0 41.0 70-71 35.5137290612641 38.5 35.0 40.5 30.0 41.0 72-73 35.18065185499211 37.0 35.0 40.0 30.0 41.0 74-75 34.82367255526488 37.0 35.0 39.0 30.0 41.0 76-77 34.438819565690316 36.5 35.0 39.0 29.0 41.0 78-79 34.0676802976466 36.0 35.0 39.0 29.0 40.5 80-81 33.78221901792239 35.5 35.0 37.0 29.0 39.0 82-83 33.5231811259922 35.0 35.0 37.0 29.0 39.0 84-85 33.23317928522421 35.0 35.0 37.0 29.0 39.0 86-87 33.00045182487045 35.0 35.0 36.0 29.0 37.0 88-89 32.777593391085105 35.0 34.0 36.0 29.0 37.0 90-91 32.59059925365225 35.0 34.0 36.0 29.0 37.0 92-93 32.4424327701327 35.0 34.0 35.0 29.0 36.0 94-95 32.3401753192059 35.0 34.0 35.0 29.0 36.0 96-97 32.22722746872088 35.0 34.0 35.0 29.0 36.0 98-99 32.1254664673431 35.0 34.0 35.0 27.5 36.0 100-101 32.08503790308636 35.0 34.0 35.0 27.0 36.0 102-103 31.268117619496522 34.5 32.5 35.0 25.5 35.0 104-105 31.924701433009993 35.0 34.0 35.0 27.0 35.0 106-107 31.992312004596343 35.0 34.0 35.0 27.0 35.0 108-109 31.991097376626712 35.0 34.0 35.0 27.0 35.0 110-111 31.964151043380767 35.0 34.0 35.0 27.0 35.0 112-113 31.895677263168466 35.0 34.0 35.0 27.0 35.0 114-115 31.840164163036263 35.0 34.0 35.0 27.0 35.0 116-117 31.78695202289246 35.0 34.0 35.0 26.0 35.0 118-119 31.712301908262816 35.0 34.0 35.0 25.0 35.0 120-121 31.615640670932045 35.0 34.0 35.0 25.0 35.0 122-123 31.45854088457269 35.0 34.0 35.0 24.5 35.0 124-125 29.50995409236193 33.5 29.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.012882674636053082 1101 2 -0.03156640000486988 1101 3 0.027656714252678682 1101 4 -0.045606844198793794 1101 5 -0.058181502965240384 1101 6 0.031955897831686286 1101 7 0.019960688990529718 1101 8 0.05495884387708827 1101 9 0.005878309220705091 1101 10-11 0.004655817010956298 1101 12-13 -0.009606500453763545 1101 14-15 0.052853753689561245 1101 16-17 0.040888070947318056 1101 18-19 0.13571918300378627 1101 20-21 -0.018620329763209043 1101 22-23 0.039224729002093284 1101 24-25 0.08691093720942433 1101 26-27 0.13578971194105804 1101 28-29 0.18128671703608035 1101 30-31 0.17866881795048073 1101 32-33 0.12726186858189692 1101 34-35 0.1453903307392963 1101 36-37 0.1359087936771175 1101 38-39 -0.0854801624494641 1101 40-41 0.1418517938657402 1101 42-43 0.1870462358100724 1101 44-45 0.16580540267288768 1101 46-47 0.21355516897390459 1101 48-49 -0.24460623929915215 1101 50-51 -0.2761430736796271 1101 52-53 -0.10664069221100902 1101 54-55 -0.27221415368229884 1101 56-57 -0.2512263354750175 1101 58-59 -0.09181300888520383 1101 60-61 -0.09065316593433437 1101 62-63 -0.15414291198195684 1101 64-65 0.05209837649854876 1101 66-67 -0.12015371298664945 1101 68-69 -6.300319705019319E-4 1101 70-71 -0.1251917795636075 1101 72-73 -0.08932095135811835 1101 74-75 0.03790628112135863 1101 76-77 0.3145049271728695 1101 78-79 0.1087479916044174 1101 80-81 0.0094066232549892 1101 82-83 0.08388665840226395 1101 84-85 0.18981756484021872 1101 86-87 0.1411114066784407 1101 88-89 -0.05184194764169092 1101 90-91 -0.018100871115343864 1101 92-93 0.08156548863614432 1101 94-95 0.05910502837509313 1101 96-97 -0.014519676729229047 1101 98-99 0.010676496973047733 1101 100-101 0.05104833291222377 1101 102-103 0.056039643633784664 1101 104-105 0.036697306119954476 1101 106-107 0.07789383892041357 1101 108-109 0.09606088795056067 1101 110-111 0.024603504493015294 1101 112-113 0.07601595707957287 1101 114-115 0.0636052097869566 1101 116-117 0.19115631788373832 1101 118-119 -0.019352516586327084 1101 120-121 0.03524731090045918 1101 122-123 0.11841835593545369 1101 124-125 0.17640246110410018 1102 1 0.08953424624406381 1102 2 0.2362136674969122 1102 3 0.14244625796656862 1102 4 0.12029988650802892 1102 5 -0.009404972816604129 1102 6 0.04893275899868854 1102 7 0.04698979913526813 1102 8 0.06597656309562439 1102 9 0.03863127865018612 1102 10-11 0.20773315198857034 1102 12-13 0.14907745813648177 1102 14-15 0.17225159233433374 1102 16-17 0.261112644193247 1102 18-19 0.22023028185495264 1102 20-21 0.2098066839318662 1102 22-23 0.25858727524730796 1102 24-25 0.3496283708434831 1102 26-27 0.33993798869837377 1102 28-29 0.31475892122897875 1102 30-31 0.2694615582633304 1102 32-33 0.4059744667515588 1102 34-35 0.6788674028493773 1102 36-37 0.65521008529781 1102 38-39 0.5836049125164564 1102 40-41 0.5470551916719373 1102 42-43 0.6716940420020663 1102 44-45 0.3555547860700443 1102 46-47 0.2000811798780333 1102 48-49 0.2684722315257986 1102 50-51 0.3221462590911841 1102 52-53 0.40809084215648994 1102 54-55 0.28234505641700736 1102 56-57 0.38977288565823187 1102 58-59 0.3757828890832471 1102 60-61 0.3423602370642982 1102 62-63 0.27028875868722224 1102 64-65 0.3252554641859646 1102 66-67 0.3336031020973991 1102 68-69 0.26924729651653934 1102 70-71 0.29294405155211223 1102 72-73 0.30499859495200354 1102 74-75 0.3138137420157605 1102 76-77 0.4409713872234988 1102 78-79 0.28749182557599084 1102 80-81 0.4151901145251884 1102 82-83 0.31188367274639006 1102 84-85 0.3801206738166556 1102 86-87 0.36512484212985896 1102 88-89 0.4132733408635332 1102 90-91 0.42957713238956075 1102 92-93 0.40417250168216157 1102 94-95 0.3985732895550811 1102 96-97 0.39612767512382874 1102 98-99 0.5613271752361726 1102 100-101 0.42931592231963833 1102 102-103 0.46997689618132554 1102 104-105 0.4357874112669684 1102 106-107 0.45804368963762343 1102 108-109 0.5699729084607164 1102 110-111 0.37836924409189976 1102 112-113 0.40857380416206723 1102 114-115 0.4938073906138527 1102 116-117 0.5489543155471424 1102 118-119 0.5303793423089793 1102 120-121 0.4235854039750322 1102 122-123 0.36951566559989146 1102 124-125 0.5375897461044232 1103 1 0.08090269382471149 1103 2 0.09092636910981611 1103 3 0.04321490716012022 1103 4 0.02207660021770863 1103 5 -0.0306373316875721 1103 6 0.04307338399868854 1103 7 0.006100182199787696 1103 8 0.03157636148272047 1103 9 0.20376484719857046 1103 10-11 0.11423516811760237 1103 12-13 0.11420472628164902 1103 14-15 0.11668203588271808 1103 16-17 0.18938255346743915 1103 18-19 0.1586438403226964 1103 20-21 0.10150275248025054 1103 22-23 0.10551897887633999 1103 24-25 0.19605604221445105 1103 26-27 0.06281475281127769 1103 28-29 0.23004999985801078 1103 30-31 0.09761806027945852 1103 32-33 0.058664738928982274 1103 34-35 0.11888756413969759 1103 36-37 0.08297596231393811 1103 38-39 0.08543202945194395 1103 40-41 0.0017867944945137992 1103 42-43 0.07712498958270686 1103 44-45 0.10382217518294823 1103 46-47 -0.16877592697679944 1103 48-49 -0.14363714347420142 1103 50-51 -0.09601150300558459 1103 52-53 -0.1673879884886773 1103 54-55 -0.1939339254378325 1103 56-57 -0.17902751756757596 1103 58-59 -0.18344090123932943 1103 60-61 -0.10651199075827833 1103 62-63 -0.09412656389342544 1103 64-65 -0.20139524145919552 1103 66-67 -0.0380261821767931 1103 68-69 0.018144725952026874 1103 70-71 -0.1299390129640159 1103 72-73 -0.025331546177028486 1103 74-75 -0.0709833850003676 1103 76-77 -0.28590613293779654 1103 78-79 -0.23667022079497713 1103 80-81 -0.3285409842651319 1103 82-83 -0.21259339378586617 1103 84-85 -0.1975947999736647 1103 86-87 -0.1970916397249809 1103 88-89 -0.2822281712332426 1103 90-91 -0.1327968595459268 1103 92-93 -0.1326848571888064 1103 94-95 -0.11305095439653456 1103 96-97 -0.12334057084391503 1103 98-99 -0.14038023403801958 1103 100-101 -0.14877757566423355 1103 102-103 -0.21386886994770649 1103 104-105 -0.23426047179755116 1103 106-107 -0.07994774181399222 1103 108-109 0.06597215241233201 1103 110-111 0.046054475946739615 1103 112-113 -0.025996508337932767 1103 114-115 0.20773758214610893 1103 116-117 0.09834947683746265 1103 118-119 0.20036422134123555 1103 120-121 0.09826408341051973 1103 122-123 0.13665276237408364 1103 124-125 0.45036066344313497 1104 1 -0.012824856462643197 1104 2 0.09971168999103952 1104 3 -0.14742056937244996 1104 4 -0.14569010236849778 1104 5 -0.0885052076454258 1104 6 -0.07439099579057995 1104 7 -0.06272315352818225 1104 8 -0.007531037942570151 1104 9 0.057340338098953225 1104 10-11 -4.379640663074724E-4 1104 12-13 0.0676327974023394 1104 14-15 0.04895873607429024 1104 16-17 0.10547675246169064 1104 18-19 -0.032743701583427764 1104 20-21 0.14709811885956015 1104 22-23 0.18367765344530795 1104 24-25 0.3030102148677045 1104 26-27 0.27383831004307524 1104 28-29 0.24248339904383442 1104 30-31 0.21347728561470092 1104 32-33 0.3398361649347308 1104 34-35 0.36355949900560347 1104 36-37 0.2253033988560844 1104 38-39 0.03203912954773358 1104 40-41 0.2289741448297633 1104 42-43 0.2854508396784965 1104 44-45 0.21294648672509453 1104 46-47 -0.025760032580244285 1104 48-49 -0.03899903284202111 1104 50-51 -0.1674988713006087 1104 52-53 -0.394324650366066 1104 54-55 -0.3105938571907103 1104 56-57 -0.3515909754219848 1104 58-59 -0.31822897595197475 1104 60-61 -0.27334703026977536 1104 62-63 -0.22780711944898258 1104 64-65 -0.35335734634425364 1104 66-67 -0.2850665617266017 1104 68-69 -0.3339228925824571 1104 70-71 -0.21405060300232748 1104 72-73 -0.1526588330544243 1104 74-75 -0.3131521769064918 1104 76-77 -0.33718875985350394 1104 78-79 -0.3748220107853939 1104 80-81 -0.35882190500267797 1104 82-83 -0.3129772838720726 1104 84-85 -0.24362793574952235 1104 86-87 -0.2569088990640651 1104 88-89 -0.32756910753592194 1104 90-91 -0.3767354371321332 1104 92-93 -0.4046930588171591 1104 94-95 -0.35450883873561523 1104 96-97 -0.3344950847328043 1104 98-99 -0.40133195218935924 1104 100-101 -0.3599058982217045 1104 102-103 -0.4599027540473237 1104 104-105 -0.5106538506289695 1104 106-107 -0.5980796263925363 1104 108-109 -0.5141097142064446 1104 110-111 -0.45565544383295276 1104 112-113 -0.36980263261953894 1104 114-115 -0.46418605889795117 1104 116-117 -0.38201450783686397 1104 118-119 -0.36834686582351495 1104 120-121 -0.5325541337829662 1104 122-123 -0.36262884682131613 1104 124-125 -0.3268970293250639 1105 1 0.04654763120656469 1105 2 0.09280934406255881 1105 3 -0.0024749262519776494 1105 4 0.02571332635192647 1105 5 -0.06041418069513327 1105 6 0.08591291022742098 1105 7 0.10312508177445778 1105 8 0.16105858029179387 1105 9 0.25567957427796273 1105 10-11 0.2631747947716647 1105 12-13 0.26897234501984 1105 14-15 0.24834998248006457 1105 16-17 0.31611599805629 1105 18-19 0.3089513928983081 1105 20-21 0.3006735050794944 1105 22-23 0.28549630933475356 1105 24-25 0.23464041426076676 1105 26-27 0.3215336652640133 1105 28-29 0.3259543447304125 1105 30-31 0.06512935812916965 1105 32-33 0.24120070005847083 1105 34-35 0.21467560761796278 1105 36-37 0.20708062618444956 1105 38-39 0.0010636752458950127 1105 40-41 -0.046973026512105776 1105 42-43 -0.20488189250614397 1105 44-45 -0.20508725002971318 1105 46-47 -0.3982422697225445 1105 48-49 -0.3043065964515179 1105 50-51 -0.26205666486758616 1105 52-53 -0.1318182224206268 1105 54-55 -0.3420889791949193 1105 56-57 -0.24355079261484036 1105 58-59 -0.3728755657005749 1105 60-61 -0.3237131515569587 1105 62-63 -0.35845194196525654 1105 64-65 -0.24289084046675669 1105 66-67 -0.35839221773917274 1105 68-69 -0.515739592927936 1105 70-71 -0.34381543073339316 1105 72-73 -0.4128367151278809 1105 74-75 -0.3225912979256975 1105 76-77 -0.18223543941228115 1105 78-79 -0.20036757133846095 1105 80-81 -0.3263127972377191 1105 82-83 -0.26161166233501376 1105 84-85 -0.4065736368120412 1105 86-87 -0.3967737492476644 1105 88-89 -0.3378719271406112 1105 90-91 -0.2488102692812788 1105 92-93 -0.30296855874834705 1105 94-95 -0.12811660007233883 1105 96-97 -0.14929239622198764 1105 98-99 -0.26517901416561784 1105 100-101 -0.14812591864627578 1105 102-103 -0.28235746871897405 1105 104-105 -0.22012339122099078 1105 106-107 -0.19193306199357707 1105 108-109 -0.28755270285704526 1105 110-111 -0.1407458696345465 1105 112-113 -0.1382716589759312 1105 114-115 -0.08510748224425058 1105 116-117 -0.09895676134779308 1105 118-119 -0.1022335456719965 1105 120-121 -0.24018220380592936 1105 122-123 -0.2623311695729882 1105 124-125 -0.4318481769396598 1106 1 0.002931310397748632 1106 2 -2.9261497257948577E-4 1106 3 0.015264005942889014 1106 4 0.0635311297120893 1106 5 -0.017258059683825877 1106 6 0.029035755253367768 1106 7 -0.07374386040320502 1106 8 -0.10645910200042863 1106 9 0.01695977931467496 1106 10-11 -0.0063465266389499675 1106 12-13 -0.1737788001696643 1106 14-15 -0.2246539893981776 1106 16-17 -0.05192348000634439 1106 18-19 -0.0420854726454678 1106 20-21 -0.054374178699525544 1106 22-23 -5.532648034360932E-4 1106 24-25 0.027191195538421198 1106 26-27 0.11139050772700898 1106 28-29 0.05678879316325691 1106 30-31 -0.03430565811942188 1106 32-33 0.12925886931287778 1106 34-35 0.38626818211722025 1106 36-37 0.21977152060045313 1106 38-39 0.22716160576280942 1106 40-41 0.1909290288109915 1106 42-43 0.36446844697970704 1106 44-45 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12-13 0.02375154254104217 2216 14-15 0.20370623701053603 2216 16-17 0.020777936456156 2216 18-19 -0.0374249449216677 2216 20-21 -0.0605243856776454 2216 22-23 -0.16309444922516292 2216 24-25 -0.12319178438329459 2216 26-27 -0.07889841683157783 2216 28-29 -0.12468596113822628 2216 30-31 -0.1635529336115198 2216 32-33 -0.0502770491349267 2216 34-35 -0.22013969149939783 2216 36-37 -0.11497545920862251 2216 38-39 0.0866685191135943 2216 40-41 0.11886121883662071 2216 42-43 -0.027588943687966605 2216 44-45 -0.04236996532457482 2216 46-47 0.1146988733051586 2216 48-49 0.33087824656339393 2216 50-51 0.222723811844034 2216 52-53 0.19629269760154955 2216 54-55 0.08371087014486989 2216 56-57 -0.01616332583824942 2216 58-59 -0.05020546890850852 2216 60-61 -0.09462436152895748 2216 62-63 -0.05646209020920878 2216 64-65 0.03159641737538976 2216 66-67 0.06157878727809418 2216 68-69 -0.00940902169834601 2216 70-71 0.012518693802903158 2216 72-73 0.0552517479959036 2216 74-75 -0.0858785330266798 2216 76-77 -0.07150587447914347 2216 78-79 -0.07300568837016641 2216 80-81 -0.10356594902077632 2216 82-83 -0.027444486361055453 2216 84-85 -0.11105080326313299 2216 86-87 -0.16278565993174965 2216 88-89 0.10886969060886287 2216 90-91 0.11706803707061653 2216 92-93 0.20382536367586113 2216 94-95 0.18059184400572548 2216 96-97 0.23371711242676696 2216 98-99 0.2698949656800238 2216 100-101 0.16791797179816825 2216 102-103 0.13263606426282237 2216 104-105 0.26695252162349803 2216 106-107 0.15514683055443257 2216 108-109 0.20487164724315576 2216 110-111 0.17258613713094917 2216 112-113 0.1528877139365008 2216 114-115 0.220917504608515 2216 116-117 0.04816150691264909 2216 118-119 -0.039737987305382916 2216 120-121 0.10301179252706305 2216 122-123 -0.0010352075507285008 2216 124-125 0.07036624380027945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34.0 3 2.0 4 9.0 5 20.0 6 40.0 7 124.0 8 200.0 9 383.0 10 989.0 11 1456.0 12 1505.0 13 2432.0 14 4301.0 15 4796.0 16 1677.0 17 971.0 18 725.0 19 677.0 20 610.0 21 624.0 22 609.0 23 674.0 24 771.0 25 1010.0 26 1317.0 27 1585.0 28 2050.0 29 2621.0 30 3397.0 31 4295.0 32 5426.0 33 7418.0 34 10940.0 35 17534.0 36 37820.0 37 123106.0 38 114889.0 39 1508.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09081881426188 20.15263853788348 15.64154490730668 21.11499774054796 2 13.794324856503767 21.275652217567618 43.974831681290546 20.955191244638065 3 18.775833505324282 28.72351106970933 29.483246222242055 23.017409202724338 4 11.328532461664611 18.28468313689178 39.764772163125514 30.622012238318096 5 11.654137438005451 38.48939949063938 34.08862427952281 15.767838791832359 6 26.772296999548175 37.92316745968439 21.04806635689702 14.25646918387041 7 26.30820034249441 33.56891444891311 22.77922498089506 17.34366022769742 8 25.21935818556057 35.746877667021806 21.063406090153006 17.97035805726462 9 26.599376370117085 17.932705984727203 22.503946495010403 32.96397115014531 10-11 23.647872239545276 27.497308574074175 29.087760008478693 19.767059177901857 12-13 26.156894791742204 25.209178180763413 29.460654978719607 19.173272048774773 14-15 21.590674557797325 27.786811176250747 28.521584399212372 22.100929866739556 16-17 19.391654069491782 30.085679382840695 30.3492438906026 20.173422657064926 18-19 19.687432017091254 27.36064549597541 33.787993730232664 19.163928756700674 20-21 21.278485831867872 26.6750526798751 32.697920835849374 19.348540652407657 22-23 20.045545380128214 28.058938455166526 32.72852526959769 19.166990895107567 24-25 20.026858478410023 27.30374903080776 33.85967212017426 18.809720370607955 26-27 18.95949194803456 28.027644988369694 33.42137410541465 19.591488958181095 28-29 18.774857340480718 28.35187116855299 32.556352601897665 20.316918889068628 30-31 19.616450887752197 27.3526967251064 33.17747234664451 19.853380040496894 32-33 19.01638841320221 27.818466807606278 33.88100829461213 19.284136484579385 34-35 19.455244236443857 28.13334969571547 33.518293329168365 18.893112738672304 36-37 19.178571229354112 27.87619998549698 32.96327388954276 19.981954895606144 38-39 19.81154440434421 28.498016990846363 32.249836841019004 19.44060176379042 40-41 20.95853809553028 27.038650549720263 32.97554567614755 19.02726567860191 42-43 19.483553016907177 28.264713593234898 32.722997885905855 19.528735503952074 44-45 19.57140785282781 28.430661616640542 31.985296168413534 20.01263436211811 46-47 20.84627913852058 27.898791229019427 31.111907537666017 20.143022094793974 48-49 20.53739269159327 28.265271401716934 30.709588169997716 20.487747736692082 50-51 19.918225276533555 29.394554673598368 30.48144450084508 20.205775549023 52-53 19.902327734795534 29.089572886045307 30.71795529722825 20.29014408193091 54-55 20.072738226057467 28.522700016176444 30.658130337529915 20.746431420236174 56-57 20.474499785243736 28.86491551990539 29.56078160124503 21.09980309360584 58-59 20.697623178058045 29.065866025558783 29.460236622358078 20.77627417402509 60-61 20.154317716555198 28.146458195043312 31.45105509474377 20.248168993657718 62-63 20.352479179798408 27.99375812308602 31.197810043899526 20.455952653216045 64-65 20.48454033792038 28.714446681876243 30.00326317961991 20.797749800583468 66-67 20.495557055440585 29.370290004629812 29.579886541754753 20.55426639817485 68-69 20.996469072308713 28.031968004105472 30.883763868513388 20.08779905507243 70-71 21.421658587740485 27.91064465926269 30.278262761263548 20.389433991733277 72-73 20.615764783319296 28.611531016940646 30.327628811923713 20.445075387816345 74-75 20.25862790269589 29.055128212279595 30.705822962743973 19.980420922280544 76-77 20.436289904224285 28.060416236689296 31.142865908419004 20.360427950667418 78-79 20.30618107578944 28.061531853653364 30.67305171442437 20.959235356132826 80-81 20.467387727097776 28.638584728319376 30.476703128747772 20.41732441583507 82-83 20.26392708327523 28.564954008690655 31.171871949484864 19.999246958549254 84-85 20.220696925917455 28.55756304630368 30.969666374746897 20.252073653031967 86-87 19.46402972003592 29.606103540410434 29.764381697188085 21.165485042365553 88-89 20.23059802647359 28.507360282920462 30.329581141610838 20.932460548995106 90-91 20.3753803588412 28.578162173016903 30.932412569761507 20.11404489838039 92-93 19.931891584343433 29.057498898328248 31.027539004758108 19.983070512570215 94-95 19.7823973939006 29.410238924984 30.39087307397847 20.416490607136932 96-97 20.59136066223023 28.92376431476017 30.13811338015206 20.34676164285754 98-99 20.589387839343562 28.3916924540116 30.691984052055343 20.326935654589494 100-101 20.02462724448188 28.42020270760237 30.82742521182777 20.72774483608798 102-103 19.9533950363343 28.94472249686581 30.35639828138967 20.74548418541022 104-105 20.30886544801388 28.493771929335242 30.129860712114198 21.067501910536677 106-107 20.18010077498839 28.290214115471457 30.178943243292245 21.350741866247912 108-109 19.74042382288465 28.86003469568758 29.856280644603483 21.543260836824285 110-111 19.44607145845536 29.052938387303712 30.175299138147437 21.325691016093494 112-113 20.009623028325173 28.328103426634872 29.83989512293767 21.822378422102283 114-115 19.826791529241135 28.763902350586772 29.01548695706745 22.393819163104645 116-117 20.19994951755799 28.575572355145372 28.66789107506683 22.556587052229805 118-119 20.05112314737858 28.042426913143643 29.85126036826516 22.05518957121262 120-121 19.64659994338236 28.122232743124652 30.171763416358594 22.059403897134395 122-123 20.060913195906767 28.470783141771 29.56688598020321 21.90141768211902 124-125 20.386510887178872 28.694207914854925 29.059995927941294 21.859285270024905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 35.0 2 107.0 3 149.5 4 64.0 5 26.5 6 62.5 7 158.5 8 307.0 9 360.0 10 286.0 11 228.5 12 224.0 13 285.5 14 361.0 15 434.5 16 515.0 17 621.0 18 711.5 19 791.0 20 929.5 21 1114.5 22 1310.5 23 1565.5 24 1886.0 25 2318.5 26 2932.0 27 3561.5 28 4263.5 29 5054.5 30 6025.5 31 7349.0 32 9033.0 33 11226.5 34 13794.0 35 16451.0 36 18379.0 37 18828.5 38 17807.5 39 15976.5 40 14142.5 41 13055.5 42 12473.0 43 11780.5 44 11283.5 45 11002.0 46 10767.0 47 10974.0 48 11804.5 49 11395.5 50 10267.5 51 9391.0 52 8281.0 53 7474.0 54 6894.5 55 6211.0 56 5290.0 57 4482.5 58 3737.5 59 2948.5 60 2325.0 61 1838.0 62 1411.0 63 1015.0 64 702.5 65 543.0 66 420.0 67 323.5 68 233.0 69 137.0 70 98.0 71 73.5 72 55.0 73 43.5 74 34.5 75 30.5 76 19.5 77 11.5 78 5.0 79 3.0 80 2.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014503020532930224 2 0.0 3 0.0 4 0.0 5 0.12550690845805001 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0034863030127236117 22-23 6.972606025447223E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.367127230536668E-4 92-93 0.0 94-95 6.972606025447223E-4 96-97 0.0 98-99 0.0012550690845805 100-101 0.0 102-103 1.3945212050894446E-4 104-105 0.0022312339281431114 106-107 0.006833153904938279 108-109 0.0 110-111 0.004462467856286223 112-113 0.008646031471554558 114-115 0.005159728458830945 116-117 0.0015339733255983889 118-119 0.0 120-121 1.3945212050894446E-4 122-123 8.367127230536668E-4 124-125 0.0013945212050894447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 358546.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.49431156902597 #Duplication Level Percentage of deduplicated Percentage of total 1 92.18773129428843 76.04963428232675 2 5.625119764986978 9.280807650118437 3 0.9735099112071032 2.409270897919607 4 0.40620925161929555 1.3403981026121214 5 0.20949359166621268 0.8641014811313427 6 0.1267730862540138 0.627483508560337 7 0.08194151704796228 0.4731796326455277 8 0.05335918597057203 0.3521463450020774 9 0.046572281355542325 0.3457753459762003 >10 0.25609594020403065 3.9496409926700067 >50 0.018968100232070302 1.1014795320531712 >100 0.012871210866133659 1.974112859314535 >500 6.774321508491399E-4 0.4219264568386337 >1k 6.774321508491399E-4 0.8100429128312578 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1730 0.48250433696094785 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1169 0.32603905774991215 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 765 0.21336174437868502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 745 0.20778365955832723 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.11211950488919135 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.789042410178889E-4 0.0 10-11 0.0 0.0 0.0 2.789042410178889E-4 0.0 12-13 0.0 0.0 0.0 2.789042410178889E-4 0.0 14-15 0.0 0.0 0.0 2.789042410178889E-4 0.0 16-17 0.0 0.0 0.0 2.789042410178889E-4 0.0 18-19 0.0 0.0 0.0 2.789042410178889E-4 0.0 20-21 0.0 0.0 0.0 2.789042410178889E-4 0.0 22-23 0.0 0.0 0.0 8.367127230536667E-4 0.0 24-25 0.0 0.0 0.0 0.0013945212050894447 0.0 26-27 0.0 0.0 0.0 0.0013945212050894447 0.0 28-29 0.0 0.0 0.0 0.0018128775666162778 0.0 30-31 0.0 0.0 0.0 0.0029284945306878338 0.0 32-33 0.0 0.0 0.0 0.006135893302393556 0.0 34-35 0.0 0.0 0.0 0.010737813279188723 0.0 36-37 0.0 0.0 0.0 0.016594802340564388 0.0 38-39 0.0 0.0 0.0 0.033329056801637724 0.0 40-41 0.0 0.0 0.0 0.05745427364968512 0.0 42-43 0.0 0.0 0.0 0.07851154384653573 0.0 44-45 0.0 0.0 0.0 0.1019395000920384 0.0 46-47 0.0 0.0 0.0 0.1295510199528094 0.0 48-49 0.0 0.0 0.0 0.1637167894775008 0.0 50-51 0.0 0.0 0.0 0.20597078199171098 0.0 52-53 0.0 0.0 0.0 0.2501771041930464 0.0 54-55 0.0 0.0 0.0 0.29549904335845334 0.0 56-57 0.0 0.0 0.0 0.3482119449108343 0.0 58-59 0.0 0.0 0.0 0.4065029312835731 0.0 60-61 0.0 0.0 0.0 0.47343994912786647 0.0 62-63 0.0 0.0 0.0 0.5421898445387761 0.0 64-65 0.0 0.0 0.0 0.6088479581420515 0.0 66-67 0.0 0.0 0.0 0.6941926558935255 0.0 68-69 0.0 0.0 0.0 0.7901357148036793 0.0 70-71 0.0 0.0 0.0 0.8984900124391291 0.0 72-73 0.0 0.0 0.0 1.0131196554974815 0.0 74-75 0.0 0.0 0.0 1.122450117976494 0.0 76-77 0.0 0.0 0.0 1.2480964785550528 0.0 78-79 0.0 0.0 0.0 1.400936002632856 0.0 80-81 0.0 0.0 0.0 1.5682785472435894 0.0 82-83 0.0 0.0 0.0 1.7466378093745294 0.0 84-85 0.0 0.0 0.0 1.9124463806596643 0.0 86-87 0.0 0.0 0.0 2.0901083821880597 0.0 88-89 0.0 0.0 0.0 2.3048646477718338 0.0 90-91 0.0 0.0 0.0 2.5196209133556087 0.0 92-93 0.0 0.0 0.0 2.7366084128675263 0.0 94-95 0.0 0.0 0.0 2.9710274274430617 0.0 96-97 0.0 0.0 0.0 3.2182760371054204 0.0 98-99 0.0 0.0 0.0 3.4734734176367885 0.0 100-101 0.0 0.0 0.0 3.7678568440311704 0.0 102-103 0.0 0.0 0.0 4.069491780692017 0.0 104-105 0.0 0.0 0.0 4.373915759763044 0.0 106-107 0.0 0.0 0.0 4.6652312395062285 0.0 108-109 0.0 0.0 0.0 4.996151121473954 0.0 110-111 0.0 0.0 0.0 5.3663965014252 0.0 112-113 0.0 0.0 0.0 5.761185454586022 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 25 0.0020480913 71.466675 2 AGTCGAT 15 0.0042366297 59.49742 70-71 TACCTGG 580 0.0 49.287365 2 GTACCTG 595 0.0 49.059456 1 ACCTGGG 640 0.0 45.59723 3 GTACACG 70 5.4056163E-5 42.551567 1 ACACGGG 80 1.1892726E-4 37.22223 3 CCTGGGG 525 0.0 36.300533 4 TAGGACC 455 0.0 35.340664 4 GTATCAA 3615 0.0 34.27667 1 GAGTACT 2155 0.0 29.679688 12-13 ATCAACG 4215 0.0 29.530514 3 TATCAAC 4210 0.0 29.424124 2 TCAACGC 4240 0.0 29.356396 4 CAACGCA 4275 0.0 29.116055 5 GTACATG 2800 0.0 28.509548 1 AACGCAG 4365 0.0 28.487883 6 TACATGG 2770 0.0 28.380268 2 GGTATCA 2580 0.0 28.169796 1 ACATGGG 2705 0.0 27.741224 3 >>END_MODULE