FastQCFastQC Report
Fri 27 May 2016
SRR939945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060232
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28550.2692806857367067No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13890.13100906216752561No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11410.1076179553154404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG551.5816113E-81361.1624108-109
CCGCTCC4750.01050.7219108-109
CCGCGCC6200.0965.9862108-109
CCGCGCT552.8946974E-5907.4416108-109
CCTCGCC5150.0823.74554108-109
CCGCTCG1850.0809.33984108-109
CGTCGCT754.2678384E-5796.79736110-111
TTGTTCG2300.0650.9907108-109
CTATTCG808.901465E-5623.8661108-109
CTCCGCC2950.0607.72687110-111
CCGCGCG3850.0583.35535108-109
GTTGTCG1101.3453166E-4543.27094110-111
CATCGCG951.489938E-4525.3609108-109
CGCCGTC1251.9733977E-4478.07843110-111
TTTTTCG4350.0451.4937492-93
CGTCGCC2804.3655746E-11445.61865108-109
CGGCGCC2854.7293724E-11437.80075108-109
CCGCTAC1152.6413868E-4433.9938108-109
CTCCGCT2201.9554827E-6407.45322110-111
CGCCGCC5200.0402.22943110-111
Produced by FastQC (version 0.11.5)