##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939945_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1060232 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.209075938096568 33.0 31.0 34.0 28.0 34.0 2 31.406788325574027 34.0 31.0 34.0 28.0 34.0 3 31.474627251394036 34.0 31.0 34.0 28.0 34.0 4 34.75877166506953 37.0 35.0 37.0 32.0 37.0 5 34.80326758671687 37.0 35.0 37.0 32.0 37.0 6 34.84408978412272 37.0 35.0 37.0 32.0 37.0 7 34.761268288450076 37.0 35.0 37.0 32.0 37.0 8 34.72919134679957 37.0 35.0 37.0 32.0 37.0 9 36.325701355929645 39.0 38.0 39.0 32.0 39.0 10-11 36.31244435180225 39.0 38.0 39.0 32.0 39.0 12-13 36.23519144866407 39.0 37.5 39.0 32.0 39.0 14-15 37.65033219144489 41.0 38.0 41.0 32.0 41.0 16-17 37.65443270906745 41.0 38.0 41.0 32.0 41.0 18-19 37.660986934934996 41.0 38.5 41.0 32.0 41.0 20-21 37.693122825947526 41.0 39.0 41.0 32.0 41.0 22-23 37.66962419545911 41.0 38.0 41.0 32.0 41.0 24-25 37.67579312829645 40.5 38.0 41.0 32.0 41.0 26-27 37.632582774336186 40.0 38.0 41.0 32.0 41.0 28-29 37.57308777701484 40.0 38.0 41.0 31.0 41.0 30-31 37.50015373993617 40.0 38.0 41.0 31.0 41.0 32-33 37.37272738419516 40.0 38.0 41.0 31.0 41.0 34-35 37.273882508733934 40.0 38.0 41.0 30.0 41.0 36-37 37.44565953489425 40.0 38.0 41.0 31.0 41.0 38-39 37.41962796821828 40.0 38.0 41.0 31.0 41.0 40-41 37.36078094228433 40.0 38.0 41.0 31.0 41.0 42-43 37.235116936670465 40.0 38.0 41.0 30.0 41.0 44-45 37.092506640056136 40.0 37.5 41.0 30.0 41.0 46-47 36.963473560503736 40.0 37.0 41.0 30.0 41.0 48-49 36.83146141599197 40.0 37.0 41.0 30.0 41.0 50-51 36.675411608025414 40.0 36.5 41.0 30.0 41.0 52-53 36.50938521002951 40.0 36.0 41.0 30.0 41.0 54-55 36.30322372839152 40.0 36.0 41.0 30.0 41.0 56-57 36.07090853699945 39.5 35.0 41.0 29.5 41.0 58-59 35.84220387613277 39.0 35.0 41.0 29.0 41.0 60-61 35.542483154630304 39.0 35.0 41.0 28.5 41.0 62-63 35.25043103773514 38.5 35.0 41.0 28.0 41.0 64-65 34.909450007168246 37.5 35.0 40.0 28.0 41.0 66-67 34.57056097156094 37.0 34.0 40.0 28.0 41.0 68-69 34.196255630843055 36.5 34.0 39.5 27.0 41.0 70-71 33.79756977718084 36.0 34.0 39.0 26.0 41.0 72-73 33.41222534313245 35.5 34.0 39.0 26.0 40.5 74-75 33.01133006738148 35.0 34.0 37.5 26.0 40.0 76-77 32.61823591440364 35.0 33.0 37.0 25.0 39.0 78-79 32.21581597235323 35.0 33.0 36.5 24.0 39.0 80-81 31.836620664156527 35.0 33.0 36.0 22.5 38.0 82-83 31.69641502991798 35.0 33.0 36.0 21.5 37.0 84-85 31.355464181424445 35.0 33.0 35.5 10.5 37.0 86-87 30.728700888107507 35.0 33.0 35.0 2.0 36.5 88-89 15.73407565513963 18.0 16.0 18.5 2.0 19.0 90-91 2.709888967697636 2.0 2.0 2.0 2.0 2.0 92-93 2.2676404786876834 2.0 2.0 2.0 2.0 2.0 94-95 2.090887654777445 2.0 2.0 2.0 2.0 2.0 96-97 2.0000146194417825 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06160907504085955 1101 2 0.1001377047992662 1101 3 0.08142419798203804 1101 4 0.04416886472128567 1101 5 0.03719451977028854 1101 6 -0.03188572371963261 1101 7 -0.03201989056786658 1101 8 0.06078816889308314 1101 9 0.1686416747169588 1101 10-11 0.04235883345577918 1101 12-13 0.09474475208038058 1101 14-15 0.1021454054400266 1101 16-17 0.1368357038092256 1101 18-19 0.12205733262622687 1101 20-21 0.13322334167358463 1101 22-23 0.17134479938412994 1101 24-25 0.15061289970341107 1101 26-27 0.13605830445723655 1101 28-29 0.20521533719098528 1101 30-31 0.14139386706451518 1101 32-33 0.1918065126046926 1101 34-35 0.23033308887600157 1101 36-37 0.3402134865575661 1101 38-39 0.24720637950261448 1101 40-41 0.23671679184412397 1101 42-43 0.2090907437390399 1101 44-45 0.1461989972334763 1101 46-47 0.20564682855028593 1101 48-49 0.17850033835133416 1101 50-51 0.09736720262647225 1101 52-53 -0.05416538368415047 1101 54-55 -0.055246190889079116 1101 56-57 0.05425172828009295 1101 58-59 -0.04495971584579195 1101 60-61 0.03809112471310527 1101 62-63 -0.027873283461282483 1101 64-65 -0.061948355176966174 1101 66-67 -0.0849059910490837 1101 68-69 -0.041859522900324464 1101 70-71 -0.11100427222239517 1101 72-73 -0.02765493840941957 1101 74-75 0.09420417224595212 1101 76-77 -0.04351606553608889 1101 78-79 0.051746216272775314 1101 80-81 0.15030842843758307 1101 82-83 0.18966244586873415 1101 84-85 0.20110596308441941 1101 86-87 0.3684280619516045 1101 88-89 0.9398411676599832 1101 90-91 0.11509423403156749 1101 92-93 -0.14112393241616905 1101 94-95 -0.0135692411206918 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.1993126805244394 1102 2 0.2149990936640016 1102 3 0.22436104405339208 1102 4 0.19920067354950532 1102 5 0.26739913965841566 1102 6 0.08283256164685326 1102 7 0.2193042696500811 1102 8 0.31938705884468277 1102 9 0.3480318726707594 1102 10-11 0.44258852362764856 1102 12-13 0.35154216824710005 1102 14-15 0.3564584361909766 1102 16-17 0.38159159460133196 1102 18-19 0.3208280916906574 1102 20-21 0.3060108529044854 1102 22-23 0.3971856682940853 1102 24-25 0.46721036948864025 1102 26-27 0.4500862513024444 1102 28-29 0.38514987186913885 1102 30-31 0.4709422034410622 1102 32-33 0.49326399456278125 1102 34-35 0.5931412142564056 1102 36-37 0.5836497457234344 1102 38-39 0.5413673944868194 1102 40-41 0.47224431843601167 1102 42-43 0.4977513312241797 1102 44-45 0.48112548913272946 1102 46-47 0.5213059741318347 1102 48-49 0.40189576773429536 1102 50-51 0.41914900420894696 1102 52-53 0.273185679991478 1102 54-55 0.27396884113711906 1102 56-57 0.12942997365006192 1102 58-59 0.19511361111128878 1102 60-61 0.2209674325703972 1102 62-63 0.2261635031949467 1102 64-65 0.07984366290841649 1102 66-67 0.15900939312040663 1102 68-69 0.1189543918317355 1102 70-71 0.1683228877347105 1102 72-73 0.22696513085991654 1102 74-75 0.2645652640350704 1102 76-77 0.18804803758538213 1102 78-79 0.20146689273568796 1102 80-81 0.19864661642123593 1102 82-83 0.19761555864153735 1102 84-85 0.1561424816480219 1102 86-87 0.36833839583561456 1102 88-89 1.1811607630574859 1102 90-91 0.1353995335707383 1102 92-93 0.05306117467596749 1102 94-95 0.11692122347658973 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.04029149140363231 1103 2 0.20471815333088017 1103 3 0.1156956424088662 1103 4 0.09443312136733795 1103 5 0.05501246455826703 1103 6 -0.14034369810435976 1103 7 -0.10054340060081302 1103 8 -0.028660063229956734 1103 9 -0.048204686469027536 1103 10-11 -0.0597781219553184 1103 12-13 0.016030624920084335 1103 14-15 0.033226831721229644 1103 16-17 0.03521008500824507 1103 18-19 0.07981238433244187 1103 20-21 0.03648470911364399 1103 22-23 0.0702591449965908 1103 24-25 0.03385858791579466 1103 26-27 0.06540224329063449 1103 28-29 0.09837857732981092 1103 30-31 0.09612009717920245 1103 32-33 0.11666953957649184 1103 34-35 0.1384197826730187 1103 36-37 0.09245614462707863 1103 38-39 0.15173794055471035 1103 40-41 0.18367563827577982 1103 42-43 0.20014301370362375 1103 44-45 0.12529189225311654 1103 46-47 0.11434571691066253 1103 48-49 0.07718830410367872 1103 50-51 0.06686405797238137 1103 52-53 0.02598585920294738 1103 54-55 0.09150389279218984 1103 56-57 0.14915377714995515 1103 58-59 0.09156627819962893 1103 60-61 0.1270645757287312 1103 62-63 0.13753684285339318 1103 64-65 0.1286366209518519 1103 66-67 0.14181299843350104 1103 68-69 0.10379257441658751 1103 70-71 0.14298027757236298 1103 72-73 0.05686050298726997 1103 74-75 0.1459812981906623 1103 76-77 0.09604271395054553 1103 78-79 -0.017194819260936356 1103 80-81 -0.05285296190472266 1103 82-83 -0.004557380426565061 1103 84-85 0.04054054194741141 1103 86-87 0.16203305111708488 1103 88-89 0.8702077797136081 1103 90-91 -0.5494715922989641 1103 92-93 -0.27926409414123654 1103 94-95 -0.09611641093201229 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.237892403513559 1104 2 0.051713521057259015 1104 3 0.08839695594398833 1104 4 -0.00665197286452468 1104 5 0.00567557769996796 1104 6 0.036520316109744044 1104 7 0.12836706326110914 1104 8 0.15730985871940817 1104 9 0.17930340414586254 1104 10-11 0.1496482375852679 1104 12-13 0.2131084851904106 1104 14-15 0.20505562796464716 1104 16-17 0.1365934215143696 1104 18-19 0.07853374990132522 1104 20-21 0.041488199182808216 1104 22-23 -0.09700083342843868 1104 24-25 0.10699140091154646 1104 26-27 0.13042540465874453 1104 28-29 0.16820851577768536 1104 30-31 0.050365290402119456 1104 32-33 0.07104481719562727 1104 34-35 0.03152530967346223 1104 36-37 0.051980860662361295 1104 38-39 0.05981269149067714 1104 40-41 0.08839357723129382 1104 42-43 0.0019134306082477792 1104 44-45 -0.03873501300736848 1104 46-47 -0.019070108966616317 1104 48-49 -0.09873973701568417 1104 50-51 -0.15287704235123556 1104 52-53 -0.0687282724043854 1104 54-55 0.014525658132626518 1104 56-57 -0.08567076755466019 1104 58-59 -0.03193775124386633 1104 60-61 -0.05206798344380559 1104 62-63 0.003099378547283038 1104 64-65 -0.10439529731706898 1104 66-67 -0.008739067052886185 1104 68-69 -0.11131625228560438 1104 70-71 -0.20170529892360634 1104 72-73 -0.08835810225418328 1104 74-75 -0.12823343624680206 1104 76-77 -0.2911519049973563 1104 78-79 -0.24245340165830243 1104 80-81 -0.2772707549118927 1104 82-83 -0.30315500717953014 1104 84-85 -0.22619909889274226 1104 86-87 -0.27762999856881265 1104 88-89 0.2994037946891783 1104 90-91 -0.5319006804390751 1104 92-93 -0.27926409414123654 1104 94-95 -0.09611641093201229 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.15059409359960085 1105 2 -0.16092143583812302 1105 3 -0.08301153276890005 1105 4 0.01302825172717803 1105 5 -0.06909153601632312 1105 6 0.006923413868634043 1105 7 4.836768968488059E-4 1105 8 0.005535153318199093 1105 9 -0.07154017451039607 1105 10-11 -0.08108459326178519 1105 12-13 0.10164794877270111 1105 14-15 -0.05043689017778519 1105 16-17 0.010768513507848354 1105 18-19 0.01646037686278845 1105 20-21 0.03501591646837454 1105 22-23 -0.14269424148621113 1105 24-25 -0.04886783598709599 1105 26-27 0.010773212191011794 1105 28-29 -0.07196904890016498 1105 30-31 -0.18695467118968168 1105 32-33 -0.19235150473866724 1105 34-35 0.04886652547270387 1105 36-37 -0.05988530477318932 1105 38-39 0.03232761232085579 1105 40-41 0.057707815249131045 1105 42-43 0.12662437547910343 1105 44-45 0.01543769416362295 1105 46-47 0.12220321888581509 1105 48-49 0.0851714973809905 1105 50-51 0.01900799246925544 1105 52-53 0.0599584225872718 1105 54-55 0.004697888339130429 1105 56-57 -0.03462845369109857 1105 58-59 -0.11252048471066445 1105 60-61 -0.10378725218192386 1105 62-63 -0.060286902029176304 1105 64-65 -0.04180437595972819 1105 66-67 0.04059558839256283 1105 68-69 0.09216433337658714 1105 70-71 0.014358572480070109 1105 72-73 -0.019315608482962432 1105 74-75 -0.010784485045704173 1105 76-77 0.17379894464794887 1105 78-79 0.1864286814213827 1105 80-81 -0.034013631300684466 1105 82-83 0.06960048784895179 1105 84-85 -0.03799497482339831 1105 86-87 0.15900568632501333 1105 88-89 0.7705238035590423 1105 90-91 -0.7063343373970037 1105 92-93 -0.27926409414123654 1105 94-95 -0.09611641093201229 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.03338672441736179 1106 2 0.05702533062611437 1106 3 0.014861604858804611 1106 4 0.005508307219322717 1106 5 -0.01157132906745062 1106 6 0.05649569557524359 1106 7 -0.0024241695646551875 1106 8 0.08218609909506114 1106 9 0.07777907368087966 1106 10-11 0.07724142873399842 1106 12-13 0.014154958976007492 1106 14-15 0.058950746104805773 1106 16-17 -0.016892971496467624 1106 18-19 0.06118819928470032 1106 20-21 0.12028058015828691 1106 22-23 0.13394503575931083 1106 24-25 0.024106513027966514 1106 26-27 -0.20614821740998934 1106 28-29 0.13414768405640842 1106 30-31 0.10860922774710957 1106 32-33 0.3016662532317582 1106 34-35 0.1184410520593886 1106 36-37 0.19714337033977358 1106 38-39 0.13848621471342426 1106 40-41 0.2712889763447137 1106 42-43 -0.1317659647534697 1106 44-45 0.0840288744497002 1106 46-47 -0.1047069176763813 1106 48-49 0.034176583487884216 1106 50-51 0.0928269978016516 1106 52-53 -0.016452894353861325 1106 54-55 0.004407184444062295 1106 56-57 -0.03198627828925282 1106 58-59 0.02894814329211215 1106 60-61 0.10141618603764613 1106 62-63 0.17446719275725542 1106 64-65 0.019082747351610863 1106 66-67 0.08087988065172169 1106 68-69 0.06480182925323419 1106 70-71 0.09000154287631545 1106 72-73 0.09443056744861167 1106 74-75 0.11906123597465523 1106 76-77 0.056637603582437634 1106 78-79 0.056082707448261715 1106 80-81 0.07995945517120617 1106 82-83 0.029622645945842407 1106 84-85 0.13310748336066425 1106 86-87 0.10919781333677392 1106 88-89 0.5780011114956256 1106 90-91 -0.2944042936743303 1106 92-93 -0.2505320516677827 1106 94-95 -0.09611641093201229 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.15470972813597683 1107 2 0.07307313998731857 1107 3 0.1701901052306667 1107 4 0.2208917243684425 1107 5 0.09629600323861354 1107 6 0.1402601978957705 1107 7 0.1690029183342574 1107 8 0.15749699127889016 1107 9 0.3681767316238833 1107 10-11 0.16832924976272778 1107 12-13 0.21201437294252656 1107 14-15 0.3462627714434987 1107 16-17 0.18816644277547567 1107 18-19 0.2331782376012157 1107 20-21 0.2047985464660229 1107 22-23 0.3137323859637178 1107 24-25 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20-21 -0.756215024288494 2216 22-23 -0.8037905626057551 2216 24-25 -0.8306107963291325 2216 26-27 -0.703988451583804 2216 28-29 -0.6233921485043865 2216 30-31 -0.8104846499828184 2216 32-33 -0.8987404621434933 2216 34-35 -0.7066341818952608 2216 36-37 -0.8646559984757758 2216 38-39 -0.8336679761979582 2216 40-41 -0.9271968247445201 2216 42-43 -0.8957386942427092 2216 44-45 -0.8228236478225597 2216 46-47 -0.7954119800277581 2216 48-49 -0.7682902820676318 2216 50-51 -0.776008749045161 2216 52-53 -0.7987582928438357 2216 54-55 -0.77914024205473 2216 56-57 -0.9566888011094221 2216 58-59 -0.7664649825502892 2216 60-61 -0.8017140190425067 2216 62-63 -0.7734474192725074 2216 64-65 -0.6873022765369683 2216 66-67 -0.7074839571908598 2216 68-69 -0.7453990320505568 2216 70-71 -0.6453797155476977 2216 72-73 -0.48442776670658105 2216 74-75 -0.48952889272780453 2216 76-77 -0.6009569850518588 2216 78-79 -0.6526555605750062 2216 80-81 -0.5904733609415302 2216 82-83 -0.5598388131751015 2216 84-85 -0.6837521137113427 2216 86-87 -0.7463422068808541 2216 88-89 -1.129181625171153 2216 90-91 -0.7063343373970037 2216 92-93 -0.27926409414123654 2216 94-95 -0.09611641093201229 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 20430.0 3 3513.0 4 2720.0 5 2448.0 6 2393.0 7 2220.0 8 2446.0 9 2651.0 10 3262.0 11 3755.0 12 4574.0 13 4751.0 14 5229.0 15 5447.0 16 6217.0 17 7248.0 18 9389.0 19 11430.0 20 11812.0 21 15856.0 22 18800.0 23 24671.0 24 37403.0 25 63764.0 26 137208.0 27 348541.0 28 292091.0 29 9668.0 30 295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.385379803665614 18.370601905997933 13.573632940714864 23.670385349621593 2 14.98723852174356 20.00863969998736 39.694894088219264 25.309227690049816 3 19.68378820074807 24.57416332954644 27.846396853734113 27.895651615971374 4 12.694692144373674 16.05718329794763 36.10540221750413 35.14272234017457 5 14.186146581562747 38.21523340039482 32.936877904058036 14.661742113984397 6 32.89959178745784 35.35103637694391 17.392325453297016 14.357046382301233 7 30.136894566472243 32.08080872865561 19.793592345826198 17.988704359045947 8 27.110481479525234 34.902643949626125 19.15693923594081 18.829935334907834 9 26.86063050351244 17.50597982328396 19.482245395347434 36.15114427785617 10-11 25.652456462030663 26.805844802607755 26.21728993118435 21.32440880417723 12-13 26.355279992869367 23.977695079627754 26.644608237295376 23.0224166902075 14-15 23.017792332244262 25.40769378777475 24.595324419561003 26.979189460419985 16-17 23.34602714983341 28.714922033242868 25.12102355390706 22.818027263016663 18-19 23.395940589763374 27.772380239097338 26.26811765083186 22.563561520307427 20-21 24.22923506880221 26.691994910456675 25.963745021047657 23.115024999693457 22-23 23.85361814026619 26.7554023808109 26.03055760700303 23.360421871919883 24-25 23.812807008277435 26.79196628662406 26.0362354654453 23.358991239653207 26-27 23.348568992446936 27.097229662941697 26.13112035856303 23.423080986048337 28-29 23.179202091637414 27.485658663940193 26.008986797104754 23.32615244731764 30-31 23.279386021172723 26.77461159444348 26.07896196304205 23.867040421341745 32-33 23.017338341716005 27.12091094086383 26.0114740066354 23.850276710784765 34-35 23.975199744206687 26.85900525451529 25.83102173017012 23.3347732711079 36-37 23.666881871442104 26.518478764759173 26.080177848118876 23.734461515679843 38-39 23.3984637324661 26.989375910178147 26.0884410204559 23.52371933689985 40-41 23.50183733371564 26.850727010691998 26.156916599385795 23.490519056206566 42-43 23.348946268363903 26.783053143085667 25.89593598382241 23.972064604728022 44-45 23.487167878667837 26.756647142601132 25.963045754223142 23.79313922450789 46-47 23.722638064121814 27.0617657267466 25.688387069999774 23.527209139131813 48-49 23.6554692588074 26.91900487676972 25.713465138005372 23.712060726417512 50-51 23.217182654362443 27.047476401391396 26.138052803537338 23.597288140708823 52-53 23.839829395830346 26.98041560715013 25.683199526141447 23.49655547087807 54-55 23.15232882449093 26.81682831657412 26.059103217938283 23.971739640996667 56-57 23.55932475156381 26.950563650219948 25.934182329905152 23.555929268311086 58-59 23.83841461114171 26.51641338876774 25.777942940790314 23.86722905930023 60-61 23.293533868059065 26.80102090863132 26.429734246844088 23.47571097646553 62-63 23.26340992509655 26.955339470672207 26.270536198933915 23.510714405297335 64-65 23.037856844404665 27.018687436983075 26.092662825365416 23.85079289324685 66-67 23.092351485335286 27.12057361030416 25.819726248594648 23.967348655765907 68-69 23.277482165401135 26.67557040769985 26.254717733016985 23.792229693882028 70-71 23.285563914313094 26.901753578462074 26.005770435150044 23.80691207207479 72-73 23.292590678266645 26.669210135140233 26.256281644017538 23.781917542575588 74-75 23.03717488247855 27.285018750613073 26.04038550053196 23.637420866376416 76-77 23.326215394366518 26.588567407888085 26.145834119324828 23.939383078420573 78-79 23.34014632687844 26.965683828682295 26.172836245754453 23.521333598684812 80-81 23.415535467708953 27.082893178096867 26.00982615125746 23.491745202936716 82-83 23.33101621729707 27.029472961425206 26.15819646000004 23.48131436127769 84-85 23.128261671277297 27.05863156236707 26.17726702954356 23.635839736812073 86-87 23.284426886268577 26.9744105359116 26.170710554351146 23.57045202346868 88-89 32.40512439896523 17.20293125975488 31.94190419941615 18.450040141863735 90-91 30.774801794666757 12.350894552781138 14.494190472720438 42.38011317983167 92-93 23.27877508337081 15.62905343399221 32.12940003841499 28.96277144422198 94-95 15.370095944330304 6.294711600324843 25.39052214201533 52.944670313329524 96-97 25.977848764762378 6.967558703886309 13.385044574393964 53.66954795695735 98-99 57.56125941136207 14.209445585215605 18.394934976043807 9.834360027378509 100-101 31.329031248012445 7.02789179880331 11.184019334921745 50.459057618262506 102-103 24.109769435307875 2.086806884523758 34.99956070412564 38.80386297604272 104-105 44.6172565002934 5.265101734348958 34.7550332865266 15.362608478831044 106-107 5.3438062694385335 1.582194303486659 5.903942953020135 87.17005647405468 108-109 17.737840106874543 8.553416769721181 17.98554862770235 55.723194495701925 110-111 40.14070527204487 2.44480817165796 21.623702998792695 35.790783557504476 112-113 28.877738528421443 2.9367792277839513 35.742624833786245 32.44285741000836 114-115 28.478820392514248 4.018767777235882 20.863132638204103 46.63927919204577 116-117 19.390024995754512 4.0511086690778235 18.383738865240463 58.175127469927205 118-119 19.836762702525462 4.2004279528237785 13.35983530915028 62.60297403550048 120-121 25.6385943463884 4.287859607914863 26.929500724390042 43.1440453213067 122-123 34.48064717449925 1.56867593781033 31.360958358356 32.58971852933442 124-125 38.58093273208335 1.639721094169029 16.84237809694352 42.936968076804106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 35.0 1 96.0 2 227.0 3 381.0 4 487.0 5 517.5 6 477.0 7 388.5 8 310.0 9 258.5 10 230.5 11 220.0 12 216.0 13 210.0 14 204.0 15 211.5 16 251.0 17 300.0 18 362.0 19 469.5 20 666.0 21 964.0 22 1372.5 23 1919.5 24 2669.5 25 3706.0 26 4960.0 27 6171.0 28 7558.0 29 9164.5 30 10775.0 31 12648.0 32 14795.5 33 16787.0 34 18744.0 35 20863.0 36 22865.0 37 24904.5 38 27243.5 39 29340.0 40 31296.5 41 33300.0 42 35431.5 43 38570.0 44 41717.5 45 43530.5 46 43943.0 47 44248.5 48 44687.0 49 45038.0 50 45350.0 51 44521.5 52 42521.0 53 39903.0 54 36835.0 55 33559.5 56 30173.5 57 26579.5 58 23074.5 59 19699.5 60 16417.0 61 13413.0 62 10671.0 63 8198.5 64 6217.5 65 4689.5 66 3470.5 67 2603.5 68 1900.0 69 1341.5 70 961.5 71 660.5 72 490.5 73 363.5 74 276.5 75 202.5 76 128.0 77 83.5 78 52.0 79 33.0 80 20.5 81 10.5 82 8.5 83 9.5 84 6.0 85 7.0 86 9.5 87 6.0 88 5.0 89 6.0 90 5.5 91 5.5 92 6.0 93 3.5 94 2.5 95 2.5 96 4.5 97 6.0 98 3.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 9.431897924227906E-4 3 0.041123074949633666 4 0.0454617479947785 5 0.04791404145507776 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.545518339382324E-4 12-13 0.0016505821367398834 14-15 0.0 16-17 4.244354065902557E-4 18-19 0.007356880380897765 20-21 0.0017920606056033021 22-23 4.715948962113953E-4 24-25 0.0 26-27 0.0 28-29 9.431897924227906E-4 30-31 0.0 32-33 8.960303028016511E-4 34-35 9.431897924227905E-5 36-37 1.4147846886341858E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.886379584845581E-4 46-47 0.0 48-49 1.4147846886341858E-4 50-51 0.0 52-53 0.0 54-55 0.0017920606056033021 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.7159489621139524E-5 64-65 9.431897924227905E-5 66-67 0.0 68-69 3.301164273479767E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.8295693772683716E-4 80-81 0.0 82-83 5.659138754536743E-4 84-85 9.431897924227905E-5 86-87 9.9034928204393E-4 88-89 19.113599664978985 90-91 26.22308136332425 92-93 40.33706773611813 94-95 44.658669046020115 96-97 48.49150940548861 98-99 93.10999856635152 100-101 51.80370899953972 102-103 53.3016830278656 104-105 74.8451753955738 106-107 81.56177138588535 108-109 12.59516785005546 110-111 38.94633438719073 112-113 52.08543979053641 114-115 86.75256924899456 116-117 66.39787329565604 118-119 72.09789932769432 120-121 80.40419455364486 122-123 75.9704479774238 124-125 50.14624157731515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1060232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.532371045877866 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0347547630091 38.892212075009674 2 13.524177753773111 14.209142477032325 3 5.0859835550930494 8.015363257481432 4 2.44691816206439 5.141696512338561 5 1.3591881075151093 3.5700686992564132 6 0.853283644312802 2.6895007806251394 7 0.5337603517043815 1.9627787791718987 8 0.38484614878987833 1.6173504547045607 9 0.2781429214524403 1.315035643817165 >10 1.3574460846800074 12.632990350244885 >50 0.09021934921778083 3.264344763974831 >100 0.04751390621062465 5.009649238607856 >500 0.0030480612863976932 1.0782796213419454 >1k 7.171908909171043E-4 0.6015873463933443 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2855 0.2692806857367067 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1389 0.13100906216752561 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1141 0.1076179553154404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.431897924227905E-5 0.0 0.0 0.0 0.0 6 9.431897924227905E-5 0.0 0.0 0.0 0.0 7 9.431897924227905E-5 0.0 0.0 0.0 0.0 8 9.431897924227905E-5 0.0 0.0 0.0 0.0 9 9.431897924227905E-5 0.0 0.0 0.0 0.0 10-11 9.431897924227905E-5 0.0 0.0 1.4147846886341858E-4 0.0 12-13 1.886379584845581E-4 9.431897924227905E-5 0.0 2.3579744810569761E-4 0.0 14-15 2.8295693772683716E-4 9.431897924227905E-5 0.0 2.8295693772683716E-4 0.0 16-17 2.8295693772683716E-4 9.431897924227905E-5 0.0 2.8295693772683716E-4 0.0 18-19 3.3011642734797665E-4 9.431897924227905E-5 0.0 2.8295693772683716E-4 0.0 20-21 3.772759169691162E-4 9.431897924227905E-5 0.0 4.2443540659025574E-4 0.0 22-23 3.772759169691162E-4 9.431897924227905E-5 0.0 7.073923443170929E-4 0.0 24-25 5.659138754536743E-4 9.431897924227905E-5 0.0 9.9034928204393E-4 0.0 26-27 5.659138754536743E-4 9.431897924227905E-5 0.0 0.0012261467301496276 0.0 28-29 6.130733650748138E-4 9.431897924227905E-5 0.0 0.002593771929162674 0.0 30-31 6.602328546959533E-4 9.431897924227905E-5 0.0 0.006508009567717254 0.0 32-33 6.602328546959533E-4 9.431897924227905E-5 0.0 0.013676251990130462 0.0 34-35 7.073923443170929E-4 9.431897924227905E-5 0.0 0.023485425831327483 0.0 36-37 7.545518339382324E-4 9.431897924227905E-5 0.0 0.037633272717669336 0.0 38-39 0.0011318277509073486 9.431897924227905E-5 0.0 0.06446702231209772 0.0 40-41 0.0011318277509073486 9.431897924227905E-5 0.0 0.09573376393091323 0.0 42-43 0.001178987240528488 9.431897924227905E-5 0.0 0.12912268258268003 0.0 44-45 0.0013204657093919066 9.431897924227905E-5 0.0 0.16524685163247288 0.0 46-47 0.0019335390744667205 9.431897924227905E-5 0.0 0.20646424556134885 0.0 48-49 0.002169336522572418 9.431897924227905E-5 0.0 0.2548027224230168 0.0 50-51 0.002169336522572418 9.431897924227905E-5 0.0 0.3121015023127014 0.0 52-53 0.002169336522572418 9.431897924227905E-5 0.0 0.3710508643391258 0.0 54-55 0.002169336522572418 9.431897924227905E-5 0.0 0.43042466177214045 0.0 56-57 0.002169336522572418 9.431897924227905E-5 0.0 0.5010695772246074 0.0 58-59 0.002169336522572418 9.431897924227905E-5 0.0 0.5761003252118404 0.0 60-61 0.002169336522572418 9.431897924227905E-5 0.0 0.6673067781391242 0.0 62-63 0.0022636555018146973 9.431897924227905E-5 0.0 0.7548819503655804 0.0 64-65 0.0022636555018146973 9.431897924227905E-5 0.0 0.8430230364674902 0.0 66-67 0.0024522934602992552 9.431897924227905E-5 0.0 0.946726754144376 0.0 68-69 0.0025466124395415344 9.431897924227905E-5 0.0 1.0675022070641143 0.0 70-71 0.002640931418783813 9.431897924227905E-5 0.0 1.2031328992145114 0.0 72-73 0.0028295693772683716 9.431897924227905E-5 0.0 1.3397067811573315 0.0 74-75 0.0029710478461317897 9.431897924227905E-5 0.0 1.4781198831953761 0.0 76-77 0.0030182073357529295 9.431897924227905E-5 0.0 1.6320484573187755 0.0 78-79 0.0030182073357529295 9.431897924227905E-5 0.0 1.674114722060832 0.0 80-81 0.0030182073357529295 1.4147846886341858E-4 0.0 1.6750107523636337 0.0 82-83 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 84-85 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 86-87 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 88-89 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 90-91 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 92-93 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 94-95 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 96-97 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 98-99 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 100-101 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 102-103 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 104-105 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 106-107 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 108-109 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 110-111 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 112-113 0.0030182073357529295 1.886379584845581E-4 0.0 1.6750107523636337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGCG 55 1.5816113E-8 1361.1624 108-109 CCGCTCC 475 0.0 1050.7219 108-109 CCGCGCC 620 0.0 965.9862 108-109 CCGCGCT 55 2.8946974E-5 907.4416 108-109 CCTCGCC 515 0.0 823.74554 108-109 CCGCTCG 185 0.0 809.33984 108-109 CGTCGCT 75 4.2678384E-5 796.79736 110-111 TTGTTCG 230 0.0 650.9907 108-109 CTATTCG 80 8.901465E-5 623.8661 108-109 CTCCGCC 295 0.0 607.72687 110-111 CCGCGCG 385 0.0 583.35535 108-109 GTTGTCG 110 1.3453166E-4 543.27094 110-111 CATCGCG 95 1.489938E-4 525.3609 108-109 CGCCGTC 125 1.9733977E-4 478.07843 110-111 TTTTTCG 435 0.0 451.49374 92-93 CGTCGCC 280 4.3655746E-11 445.61865 108-109 CGGCGCC 285 4.7293724E-11 437.80075 108-109 CCGCTAC 115 2.6413868E-4 433.9938 108-109 CTCCGCT 220 1.9554827E-6 407.45322 110-111 CGCCGCC 520 0.0 402.22943 110-111 >>END_MODULE