FastQCFastQC Report
Fri 27 May 2016
SRR939945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060232
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.42509563944495166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.29040813708697716No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30120.2840887654777445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.21070859962725141No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14530.13704547683903145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1051.4748661E-534.0292554
TGTACGC700.002777154634.029252
CGCTATA750.003893920231.760642
GTATCAA89200.029.5795021
GAGTACT44700.028.2834312-13
GTACATG57300.028.0647471
TATACCG850.007179912628.0240925
AGTACTT46550.027.03157212-13
TACATGG57900.026.7414682
ATACCGA900.00953252126.4422326
TAAGGTG5700.026.1189445
TATCAAC102050.025.8512292
ATCAACG101700.025.8230843
TCAACGC101850.025.7265834
ACATGGG57700.025.5956613
CAACGCA102700.025.5136575
GTACTTT49300.025.40304814-15
AACGCAG104600.025.0265876
AGGTAAG5300.024.7193622
AGAGTAC79200.024.26375210-11
Produced by FastQC (version 0.11.5)