FastQCFastQC Report
Fri 27 May 2016
SRR939944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429837
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40560.9436135093070165No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21900.5094954599068949No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15040.34990007840181275No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14500.33733717665068386No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12380.28801615496106664No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9310.2165937320426115No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8840.20565935459255485No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8510.19798202574464271No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8230.19146792854035366No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6680.15540774758803919No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6510.15145275999972083No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6290.1463345407677794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG100.00126130242539.3723108-109
CGTCGCG100.00126130242539.3723108-109
CCGCTCC1100.02308.5203108-109
CCGCTCG503.6379788E-122031.498108-109
AACGACG150.00283756271692.915108-109
TTTCGCG200.0036252321496.7844110-111
GTGCGCT200.0036252321496.7844110-111
CTCCGCC1050.01425.509110-111
CCGCGCC1250.01422.0486108-109
CTCGTAT250.00566380421197.4276110-111
CGCCGTC250.00566380421197.4276110-111
GTGCGTC250.00566380421197.4276110-111
GGTCGCC451.486361E-51128.61108-109
CGTCGCC451.486361E-51128.61108-109
CCGTACC250.0078800791015.749108-109
CGCGGCT300.008154985997.8563110-111
CTGCGCG300.008154985997.8563110-111
CGCCGCT300.008154985997.8563110-111
CAGCTCG806.4339474E-8952.2646108-109
CGTGGCC603.5216977E-5846.4575108-109
Produced by FastQC (version 0.11.5)