FastQCFastQC Report
Fri 27 May 2016
SRR939944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429837
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43471.0113135909658777No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22640.5267112882325161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.4987937287855629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.3971272831329085No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14810.34454921284114676No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14620.3401289325953792No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12600.2931343741930081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.2263648778490451No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9620.22380576823307438No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9480.22054871963092987No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9010.20961434218087321No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8890.2068225862361779No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7380.17169299059876186No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7020.1633177227646759No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6720.1563383329029376No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.15168540632844543No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC4490.10445820159734968No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC300.004236373659.494246
TCGTTCA750.003909638631.7302639
GTACATG30450.030.9209371
TACATGG30850.030.3197822
ACATGGG29900.028.8920563
GAGTACT28950.028.66821112-13
AGTACTT29000.028.3110512-13
CATGGGG14250.027.5937484
CGGTTAC656.3806285E-527.45888128-29
CGAAACA557.7540183E-427.0428452-53
GTGTAGC3750.027.0147191
GTACTTT30750.026.79815314-15
GATCACG450.00953214826.44188548-49
TCGAAAC450.00953214826.44188552-53
GTCCTAC4300.024.9451921
AGAGTAC46250.024.76246610-11
TACCTGG3850.024.7594432
TGTAGCC4400.024.3725782
TAGCCCT4700.024.0844864
TACTTTT34200.023.92085814-15
Produced by FastQC (version 0.11.5)