##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939944_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 429837 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75756391376266 34.0 31.0 34.0 31.0 34.0 2 32.80493303275428 34.0 31.0 34.0 31.0 34.0 3 32.84121190125559 34.0 31.0 34.0 31.0 34.0 4 36.28710650781575 37.0 37.0 37.0 35.0 37.0 5 36.20525920290715 37.0 37.0 37.0 35.0 37.0 6 36.357623936515466 37.0 37.0 37.0 35.0 37.0 7 36.34986750791579 37.0 37.0 37.0 35.0 37.0 8 36.36760213755447 37.0 37.0 37.0 35.0 37.0 9 38.17408226839476 39.0 39.0 39.0 37.0 39.0 10-11 38.16774963532688 39.0 39.0 39.0 37.0 39.0 12-13 38.16576981506944 39.0 39.0 39.0 37.0 39.0 14-15 39.61755735313619 41.0 40.0 41.0 37.0 41.0 16-17 39.64975327856838 41.0 39.5 41.0 37.0 41.0 18-19 39.73961757596484 41.0 40.0 41.0 37.0 41.0 20-21 39.76196325583884 41.0 40.0 41.0 37.0 41.0 22-23 39.71295514346136 41.0 40.0 41.0 37.0 41.0 24-25 39.66211494124517 41.0 40.0 41.0 37.0 41.0 26-27 39.54003378024693 41.0 40.0 41.0 36.5 41.0 28-29 39.44952272605662 41.0 39.0 41.0 36.0 41.0 30-31 39.281748662865226 41.0 39.0 41.0 35.5 41.0 32-33 39.04662930366628 41.0 39.0 41.0 35.0 41.0 34-35 38.88054076312649 41.0 38.0 41.0 35.0 41.0 36-37 38.84652554340366 41.0 38.5 41.0 35.0 41.0 38-39 38.87389173105154 41.0 39.0 41.0 35.0 41.0 40-41 38.792526004043395 41.0 39.0 41.0 35.0 41.0 42-43 38.60910298555034 41.0 39.0 41.0 35.0 41.0 44-45 38.3308312220679 41.0 39.0 41.0 35.0 41.0 46-47 37.9264593322585 41.0 38.5 41.0 34.0 41.0 48-49 37.503252395675574 41.0 38.0 41.0 33.0 41.0 50-51 37.24413324120539 41.0 38.0 41.0 33.0 41.0 52-53 37.13656804788792 41.0 38.0 41.0 32.0 41.0 54-55 36.99723499838311 40.5 38.0 41.0 32.0 41.0 56-57 36.83701030855883 40.0 37.5 41.0 31.0 41.0 58-59 36.65937436749279 40.0 37.0 41.0 31.0 41.0 60-61 36.4160728369126 40.0 36.5 41.0 31.0 41.0 62-63 36.22388835767977 40.0 36.0 41.0 31.0 41.0 64-65 35.9794061469813 39.0 36.0 41.0 30.0 41.0 66-67 35.68376151890135 39.0 35.0 41.0 30.0 41.0 68-69 35.33123486344823 38.5 35.0 41.0 29.5 41.0 70-71 34.94893762053988 37.0 35.0 40.0 29.0 41.0 72-73 34.575917149989415 37.0 35.0 39.0 29.0 41.0 74-75 34.224168696505885 36.5 35.0 39.0 29.0 41.0 76-77 33.84937080800397 36.0 35.0 39.0 28.5 40.5 78-79 33.47917582711586 35.5 34.5 37.0 28.0 39.0 80-81 33.181965489243595 35.0 34.0 37.0 28.0 39.0 82-83 32.94445219932207 35.0 34.0 36.5 28.5 39.0 84-85 32.68757924515572 35.0 34.0 36.0 27.5 37.0 86-87 32.52250620584082 35.0 34.0 36.0 27.5 37.0 88-89 32.33419296151797 35.0 34.0 35.5 27.0 37.0 90-91 32.16790085544055 35.0 34.0 35.0 27.0 36.0 92-93 32.036488250197166 35.0 34.0 35.0 26.0 36.0 94-95 31.936427064212715 35.0 34.0 35.0 26.0 36.0 96-97 31.861703389889655 35.0 34.0 35.0 26.0 36.0 98-99 31.81418421401601 35.0 34.0 35.0 26.0 36.0 100-101 31.748469768772814 35.0 34.0 35.0 25.0 35.0 102-103 30.87657414322173 34.5 32.0 35.0 23.5 35.0 104-105 31.548657979652752 35.0 34.0 35.0 25.0 35.0 106-107 31.58373639309785 35.0 34.0 35.0 25.0 35.0 108-109 31.60914602512115 35.0 34.0 35.0 25.0 35.0 110-111 31.598986359945748 35.0 34.0 35.0 25.0 35.0 112-113 31.504683170597225 35.0 34.0 35.0 25.0 35.0 114-115 31.44338318944158 35.0 34.0 35.0 24.0 35.0 116-117 31.37534693383771 35.0 34.0 35.0 24.0 35.0 118-119 31.274839066902103 35.0 34.0 35.0 21.5 35.0 120-121 31.191543538597188 35.0 34.0 35.0 19.5 35.0 122-123 31.017645060802117 35.0 34.0 35.0 10.0 35.0 124-125 29.03722690229087 33.0 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14200852159644484 1101 2 0.15220250139456937 1101 3 0.11843287456811424 1101 4 0.054016577770234164 1101 5 -0.005088755652145949 1101 6 0.03888386801116184 1101 7 0.08739986394434851 1101 8 0.06811931164929774 1101 9 0.11839723134792024 1101 10-11 0.1550540356753629 1101 12-13 0.12704751378029755 1101 14-15 0.2207127555227757 1101 16-17 0.21299906951036007 1101 18-19 0.22641836367534296 1101 20-21 0.2425080182099535 1101 22-23 0.24130023935065026 1101 24-25 0.31877166286668057 1101 26-27 0.2743375272790587 1101 28-29 0.12850239114113293 1101 30-31 0.2943103107660079 1101 32-33 0.3497503984292578 1101 34-35 0.46130278589818374 1101 36-37 0.37358979413312454 1101 38-39 0.31707562005135514 1101 40-41 0.3345978962087486 1101 42-43 0.46317475226152993 1101 44-45 0.4325747260239865 1101 46-47 0.578580587704657 1101 48-49 0.720141843179519 1101 50-51 0.8396881252274824 1101 52-53 0.8777067454832093 1101 54-55 0.5634742875918306 1101 56-57 0.6160833680886597 1101 58-59 0.6374837148466739 1101 60-61 0.6502828793670261 1101 62-63 0.7791462888931164 1101 64-65 0.7272090382990797 1101 66-67 0.8124144038136762 1101 68-69 0.6877173231223281 1101 70-71 0.7793904897189208 1101 72-73 0.6372908635542416 1101 74-75 0.6009696153957975 1101 76-77 0.5832432859840466 1101 78-79 0.7234773057471529 1101 80-81 0.7074052256053278 1101 82-83 0.49230098525577404 1101 84-85 0.436380364228242 1101 86-87 0.5933366426861824 1101 88-89 0.4077307513174304 1101 90-91 0.46026666752624834 1101 92-93 0.49565181235571387 1101 94-95 0.46683206940685906 1101 96-97 0.5072901611479566 1101 98-99 0.35400804781694006 1101 100-101 0.4404690823727009 1101 102-103 0.4469508347806759 1101 104-105 0.37061910213630966 1101 106-107 0.4544317318993478 1101 108-109 0.49944316802311306 1101 110-111 0.48901244853408343 1101 112-113 0.38763823647923346 1101 114-115 0.49860536177138215 1101 116-117 0.4718123120330233 1101 118-119 0.4057032564051859 1101 120-121 0.24763536769478023 1101 122-123 0.31193139349091936 1101 124-125 0.5193507600624159 1102 1 -0.015895405348004488 1102 2 -0.020345373552004276 1102 3 0.09543361471426692 1102 4 0.016005902986286458 1102 5 -0.05990126933442497 1102 6 -0.120059912155007 1102 7 -0.0961824434352252 1102 8 -0.16522479376419597 1102 9 -0.07265429741874385 1102 10-11 0.04549937431828255 1102 12-13 0.014817641064581721 1102 14-15 0.19709974771475913 1102 16-17 0.16165143015960126 1102 18-19 0.0093731105140904 1102 20-21 -0.09193119077187362 1102 22-23 0.03436162978295698 1102 24-25 0.09168491982249094 1102 26-27 0.16354494541993603 1102 28-29 0.14922257419699747 1102 30-31 0.08305461633170097 1102 32-33 8.522779834976291E-4 1102 34-35 0.16429177752723234 1102 36-37 0.22420927561383053 1102 38-39 0.13323921320031218 1102 40-41 -0.045315996647282475 1102 42-43 0.18440026749707528 1102 44-45 0.2711616202247811 1102 46-47 0.37104517014755345 1102 48-49 0.13803904626102792 1102 50-51 0.2113027384229298 1102 52-53 -0.018589396374665057 1102 54-55 -0.03806125506039848 1102 56-57 -0.13552723163822122 1102 58-59 -0.010417814441225914 1102 60-61 0.048856794948669346 1102 62-63 -0.06267874472619894 1102 64-65 0.16043300027016016 1102 66-67 0.31515867810558973 1102 68-69 0.27735449513853894 1102 70-71 0.07071748837415726 1102 72-73 0.0761545307178153 1102 74-75 0.12758490618983842 1102 76-77 0.1365259905406404 1102 78-79 0.12934322462296421 1102 80-81 0.0037438476825357725 1102 82-83 -0.04562498970435769 1102 84-85 0.13741083531563447 1102 86-87 0.008959981266571049 1102 88-89 -0.0958839765485493 1102 90-91 0.07345169363985349 1102 92-93 0.1304053228376354 1102 94-95 0.2109330524460482 1102 96-97 0.11367574601978703 1102 98-99 0.05470493190588144 1102 100-101 0.02213665250415886 1102 102-103 -0.10691564702319667 1102 104-105 -0.02386055426907774 1102 106-107 -0.07608887231010897 1102 108-109 -0.08915805999401982 1102 110-111 -0.026759961736228632 1102 112-113 -0.13199518268325505 1102 114-115 -0.2029567635947842 1102 116-117 -0.07699042338036577 1102 118-119 -0.18953583165135512 1102 120-121 -0.13406243375840887 1102 122-123 -0.06743911741841657 1102 124-125 -0.021586619403219487 1103 1 0.1752314135634947 1103 2 0.26139042469769436 1103 3 0.22921487927850848 1103 4 0.19485992401057217 1103 5 0.06839866334642863 1103 6 0.19399943226009242 1103 7 0.055342594108147125 1103 8 0.08799875757959796 1103 9 0.11323975260455654 1103 10-11 0.19042454649994056 1103 12-13 0.20969491308571264 1103 14-15 0.2737671911407844 1103 16-17 0.23825155444109924 1103 18-19 0.1616438370430089 1103 20-21 0.21566810496630495 1103 22-23 0.19679236874002726 1103 24-25 0.3677011799850902 1103 26-27 0.15532165299593004 1103 28-29 0.26796189375579615 1103 30-31 0.3101233854270333 1103 32-33 0.16185636891613342 1103 34-35 0.2543117143508127 1103 36-37 0.3227295490331272 1103 38-39 0.2403374991513232 1103 40-41 0.21854397953210025 1103 42-43 0.14181831938460476 1103 44-45 0.17743654125424513 1103 46-47 0.4853401315685062 1103 48-49 0.5945806546707431 1103 50-51 0.3906681260258509 1103 52-53 0.5535560129775234 1103 54-55 0.6080886077121193 1103 56-57 0.5182788892255346 1103 58-59 0.4032868098661666 1103 60-61 0.5800980564058662 1103 62-63 0.5117943535079732 1103 64-65 0.581370548817624 1103 66-67 0.6785448310753992 1103 68-69 0.7748209650261728 1103 70-71 0.8286313716531168 1103 72-73 0.7483789106049272 1103 74-75 0.8397890903335821 1103 76-77 0.7766766818564648 1103 78-79 0.7735045834340042 1103 80-81 0.5708105454195689 1103 82-83 0.5968184363222591 1103 84-85 0.6145715209352289 1103 86-87 0.6139053491915831 1103 88-89 0.49165421411998267 1103 90-91 0.49855655615345285 1103 92-93 0.4200899585375062 1103 94-95 0.4376289770486075 1103 96-97 0.6018612361549458 1103 98-99 0.7124155631523195 1103 100-101 0.7207320126615784 1103 102-103 0.605887936426651 1103 104-105 0.49162025977472723 1103 106-107 0.46900200853500706 1103 108-109 0.5207663354282772 1103 110-111 0.564897642737904 1103 112-113 0.6657043150123556 1103 114-115 0.5381726445145851 1103 116-117 0.5924156146290613 1103 118-119 0.4436525066014525 1103 120-121 0.6261791384025379 1103 122-123 0.7961036820223129 1103 124-125 0.7769945001659373 1104 1 0.10605320065460688 1104 2 0.16167669516603667 1104 3 0.0650941113102661 1104 4 0.046709231236043536 1104 5 -0.04051389056784416 1104 6 -0.0068555622053452225 1104 7 0.02479180972706274 1104 8 0.03616471811244537 1104 9 -0.116662152048292 1104 10-11 0.05688509842940448 1104 12-13 0.14003194885043513 1104 14-15 0.10318434446724467 1104 16-17 0.11160931618773162 1104 18-19 0.08501877501774402 1104 20-21 0.04212331318035467 1104 22-23 0.10185363062025488 1104 24-25 0.17345330792890934 1104 26-27 0.12353418019162632 1104 28-29 0.13378119983618575 1104 30-31 0.033864749601235644 1104 32-33 0.15900893199105326 1104 34-35 0.022184533924068717 1104 36-37 0.12640144490701744 1104 38-39 0.2273141013117197 1104 40-41 0.12941462344981147 1104 42-43 -0.010908883943777425 1104 44-45 0.12839650155807902 1104 46-47 -0.03472170285265719 1104 48-49 -0.05613502150009708 1104 50-51 0.09159564234326467 1104 52-53 0.0013860828275156223 1104 54-55 -0.08665928342876583 1104 56-57 0.047932692805822796 1104 58-59 0.052636021668149624 1104 60-61 0.20169811747690147 1104 62-63 0.17828544095405618 1104 64-65 0.017765067692387504 1104 66-67 0.1249206469462294 1104 68-69 0.10787566177032204 1104 70-71 0.1698161496980788 1104 72-73 0.22998889571886139 1104 74-75 0.2349568448280337 1104 76-77 0.1939330656879079 1104 78-79 0.15284730643870148 1104 80-81 0.16665389821930177 1104 82-83 0.14636612898228663 1104 84-85 0.14156491762975776 1104 86-87 0.12784099559029727 1104 88-89 0.1664013418729553 1104 90-91 0.22214142580495633 1104 92-93 0.07969910392472812 1104 94-95 0.027521721046468883 1104 96-97 0.06539419542819758 1104 98-99 0.14108841326110522 1104 100-101 0.2355112027070021 1104 102-103 0.18752082317805474 1104 104-105 0.2476300311400479 1104 106-107 0.13162271924915814 1104 108-109 0.17674557127697454 1104 110-111 0.18335636253637233 1104 112-113 0.16631502534481513 1104 114-115 0.0640782134294291 1104 116-117 0.030023920289583117 1104 118-119 0.30925806406547807 1104 120-121 0.3362596758276304 1104 122-123 0.4392580008894491 1104 124-125 0.217560933999291 1105 1 0.12561871271208247 1105 2 0.096932323423772 1105 3 0.0771503507592044 1105 4 0.08033460552903904 1105 5 -0.018996509817903018 1105 6 0.10719320986716241 1105 7 0.019224547888313737 1105 8 -0.025809577354145574 1105 9 0.18448999266600907 1105 10-11 0.11098661038829505 1105 12-13 0.1317229522637433 1105 14-15 0.1337377436022109 1105 16-17 0.07548988745435281 1105 18-19 0.009067177418152994 1105 20-21 0.2211863176287423 1105 22-23 0.21022300698341922 1105 24-25 0.2267242899012345 1105 26-27 0.19611929719284404 1105 28-29 0.12473162680745986 1105 30-31 0.14053116981983038 1105 32-33 0.13348430566794178 1105 34-35 0.08603183470162179 1105 36-37 -0.13377131518811325 1105 38-39 -0.05089865730472809 1105 40-41 0.029497183552329886 1105 42-43 0.029623197433373605 1105 44-45 -0.04137787523637115 1105 46-47 0.12101006307096895 1105 48-49 -0.08902474831242557 1105 50-51 -0.1302076181646612 1105 52-53 -0.13507467647897897 1105 54-55 -0.28986926950445024 1105 56-57 -0.13336313113167364 1105 58-59 -0.20139278811091543 1105 60-61 -0.07331081626553981 1105 62-63 -0.10436338287254188 1105 64-65 -0.04230911317585395 1105 66-67 -0.12141035745286644 1105 68-69 -0.08171190738916323 1105 70-71 0.008050102848294216 1105 72-73 0.03636943617947708 1105 74-75 -0.12069359323341189 1105 76-77 -0.03365556319827334 1105 78-79 0.025210620179407783 1105 80-81 -0.2280819710646469 1105 82-83 -0.0483771337436778 1105 84-85 -0.40688677238786397 1105 86-87 -0.11548457462892259 1105 88-89 -0.23608408688507154 1105 90-91 -0.21565508202592554 1105 92-93 -0.15446352715483513 1105 94-95 -0.09656857752536396 1105 96-97 -0.04158028487443843 1105 98-99 -0.1876868630687909 1105 100-101 0.006091464681297509 1105 102-103 -0.09560364541830069 1105 104-105 -0.17820881646878917 1105 106-107 -0.13727799914695993 1105 108-109 0.03910062900743583 1105 110-111 -0.19285154105314462 1105 112-113 -0.23824549613929946 1105 114-115 -0.1494209794531507 1105 116-117 -0.087201567369533 1105 118-119 -0.1572373211944651 1105 120-121 -0.2872835080349496 1105 122-123 -0.30287845740537733 1105 124-125 -1.0813194283088023E-5 1106 1 -0.05168256941770011 1106 2 -0.05587746337776167 1106 3 -0.030793465750512894 1106 4 -0.006715516316148751 1106 5 -0.14730010639399183 1106 6 -0.049500928093429764 1106 7 -0.0334738026094783 1106 8 -0.020828007204379162 1106 9 -0.1387952965173227 1106 10-11 -0.08907829207431917 1106 12-13 0.015267116552692528 1106 14-15 -0.10428285374536017 1106 16-17 -0.14665532262144865 1106 18-19 -0.02893260215839888 1106 20-21 -1.8618576945073073E-4 1106 22-23 -0.19490306298877869 1106 24-25 -0.11943483134391641 1106 26-27 0.04874172405511956 1106 28-29 0.07273373307605624 1106 30-31 -0.12979493444046142 1106 32-33 0.09090339189025087 1106 34-35 0.08432600205200202 1106 36-37 0.03263737439442593 1106 38-39 0.14125671674163698 1106 40-41 -0.005970175407270517 1106 42-43 0.11858120676463813 1106 44-45 0.19885797786363923 1106 46-47 0.2928215765229254 1106 48-49 0.21040459984642723 1106 50-51 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80-81 -0.5988021338532903 2216 82-83 -0.5905285537206666 2216 84-85 -0.5979090673537968 2216 86-87 -0.5897303500867608 2216 88-89 -0.590722236049011 2216 90-91 -0.5346854279517856 2216 92-93 -0.541927032418922 2216 94-95 -0.5568512458435109 2216 96-97 -0.5184182924830374 2216 98-99 -0.43830837948036105 2216 100-101 -0.5007065093079106 2216 102-103 -0.5525652927685627 2216 104-105 -0.44680122556372126 2216 106-107 -0.43530396570450947 2216 108-109 -0.43209175354586904 2216 110-111 -0.5634423134144626 2216 112-113 -0.651837020513355 2216 114-115 -0.7304249164610255 2216 116-117 -0.8477894643880219 2216 118-119 -0.7687287394020181 2216 120-121 -0.9346868522798424 2216 122-123 -0.8427871099754896 2216 124-125 -0.9578118394704944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40.0 3 0.0 4 12.0 5 29.0 6 46.0 7 113.0 8 232.0 9 465.0 10 961.0 11 1551.0 12 1903.0 13 3397.0 14 5992.0 15 6667.0 16 2307.0 17 1348.0 18 1125.0 19 866.0 20 851.0 21 835.0 22 846.0 23 898.0 24 1112.0 25 1304.0 26 1653.0 27 2147.0 28 2695.0 29 3474.0 30 4408.0 31 5789.0 32 7320.0 33 9869.0 34 14331.0 35 23791.0 36 51606.0 37 154553.0 38 114226.0 39 1074.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45501081974079 18.749331037531704 14.578030109128138 21.217628033599368 2 14.408019784243795 21.120796953263678 41.708368521090556 22.762814741401975 3 20.994469996766217 26.351849654636528 28.886531406091148 23.767148942506115 4 11.684196567536066 16.969688509830473 39.36887703943588 31.977237883197585 5 11.990376396552085 39.264395229189425 33.99447085319812 14.750757521060368 6 29.0203030451078 37.76036032263393 18.29809904684799 14.921237585410282 7 27.13796159939698 34.095947998892605 20.807189702142907 17.958900699567508 8 25.690203495743734 34.20901411465277 19.536708100977812 20.56407428862569 9 26.271586671226533 17.74533136979832 21.37205498828626 34.61102697068889 10-11 24.954692127480882 26.891821783606346 26.88775047285366 21.26573561605911 12-13 27.341759783359738 24.542791802473964 27.657111881945944 20.458336532220354 14-15 22.705467421371356 25.825603659061457 28.338416655615966 23.130512263951218 16-17 20.270823591268318 29.31971887017637 28.670751936199068 21.73870560235624 18-19 19.99909267931797 27.933263074142054 31.85509856061716 20.2125456859228 20-21 22.017898252401885 25.703291040287933 31.54226725931992 20.736543447990258 22-23 20.76605961343396 26.368196242287755 31.73896555895737 21.126778585320913 24-25 21.26061739682717 26.18050563353085 32.193598968911466 20.36527800073051 26-27 20.67167321566082 25.92657216572794 33.05066804393294 20.35108657467831 28-29 19.724918980916023 27.669791106861435 31.99736179063226 20.607928121590277 30-31 20.666903965921964 26.157589970151474 32.06634142709911 21.109164636827447 32-33 20.514404297442983 26.387677189260117 32.44287951013989 20.65503900315701 34-35 21.381477164599605 26.279729292731897 32.12543359459516 20.21335994807334 36-37 20.84371517575267 26.016838941273086 32.64318799917178 20.496257883802464 38-39 20.45717330057673 27.015008014666027 32.171613890847 20.356204793910248 40-41 21.204316985275813 26.792365478076576 31.591742916500902 20.41157462014671 42-43 20.446471569455397 27.0743328284908 30.93451703785388 21.544678564199916 44-45 21.300632565367803 26.360806538292426 30.711641854935706 21.626919041404065 46-47 22.11919867298534 27.183211310333917 30.03952661125031 20.65806340543043 48-49 22.069528681802637 27.16808929896682 29.675551429960663 21.086830589269887 50-51 20.97865004641294 27.9161635689808 29.9864832483011 21.118703136305157 52-53 21.682754160297975 28.651907583572378 28.517670651898282 21.147667604231373 54-55 21.124984587180723 27.331174375402767 29.314484327780065 22.229356709636445 56-57 21.962395047424955 27.272780146892895 28.940272708026534 21.824552097655623 58-59 22.929505835933156 27.134122934973025 28.657142125968683 21.27922910312514 60-61 21.665654655136716 27.23741790492675 30.316143096103872 20.78078434383266 62-63 21.100440399500275 27.405737523759004 29.965545078715884 21.528276998024833 64-65 20.89722383135933 28.402278072850873 29.155470562096795 21.545027533693002 66-67 21.446618136642496 27.769945351377384 28.43042827862655 22.353008233353574 68-69 21.296212285122035 27.352461514481067 29.160821427657464 22.190504772739434 70-71 22.111986636794878 27.276502488152488 28.900024893157173 21.71148598189546 72-73 21.92435737267848 26.679415685480773 29.873184486212217 21.52304245562853 74-75 21.01296537989982 28.425659028887694 28.955162073064905 21.606213518147577 76-77 21.362283842479822 27.064910652177453 29.10265984547631 22.470145659866414 78-79 21.463484995475028 27.356183855740664 29.12894888062219 22.05138226816212 80-81 21.970072376272867 27.183792926155732 29.03961269039194 21.806522007179467 82-83 21.61296026168059 27.210081961301608 29.746043267564215 21.430914509453583 84-85 21.219671642971637 27.757847742283705 29.36473593478458 21.65774467996008 86-87 20.916649799807836 27.98537585177637 28.967608651651673 22.130365696764123 88-89 20.999471892833796 27.83927395733732 28.381456226430018 22.77979792339887 90-91 21.426253249812426 27.3116853658111 28.530299593446284 22.73176179093019 92-93 21.748127778669588 27.84567173137724 28.947368421052634 21.458832068900538 94-95 21.32376376981865 27.715169774448334 29.064175788383917 21.8968906673491 96-97 20.550231832066572 27.746564395340563 29.656474431005243 22.046729341587625 98-99 21.338460947887476 27.878728658689084 28.33809685003007 22.44471354339337 100-101 20.79453466608815 27.330973521327294 28.952403995472697 22.922087817111855 102-103 21.54044977671691 27.017661407678055 28.65037904035381 22.791509775251228 104-105 22.277347503309457 26.933850438197517 28.30951378338828 22.47928827510475 106-107 21.274823555141417 27.36172039408248 29.273851029004778 22.089605021771323 108-109 20.839459700909185 27.599712448468715 28.751547101685293 22.809280748936803 110-111 20.476317329291316 28.651046895375604 28.259369943359815 22.613265831973266 112-113 21.013017531206014 27.582624678633334 27.993276020520934 23.411081769639722 114-115 20.508074919995302 27.71902558870779 28.070918306215297 23.70198118508161 116-117 21.501955410589694 27.57815244525199 27.78195252574837 23.13793961840994 118-119 20.97425416408332 26.943849580014728 29.00346992402925 23.078426331872702 120-121 20.380169576895295 28.447460592390794 28.276231898556304 22.896137932157608 122-123 20.66009280337619 27.632909021749118 27.999329973117497 23.707668201757194 124-125 20.826232666132345 27.7338392692003 28.038261593814305 23.401666470853044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44.0 1 28.5 2 47.0 3 81.0 4 52.5 5 31.0 6 82.0 7 221.5 8 364.0 9 399.5 10 332.0 11 281.5 12 316.5 13 376.0 14 445.0 15 509.5 16 589.0 17 712.0 18 813.0 19 908.5 20 1045.0 21 1242.0 22 1420.5 23 1614.5 24 1898.0 25 2328.0 26 2913.5 27 3561.0 28 4171.0 29 4918.0 30 5894.0 31 6935.5 32 8103.0 33 9301.5 34 10622.0 35 11876.0 36 12939.5 37 13670.0 38 13903.0 39 13955.0 40 14054.0 41 14449.5 42 14756.5 43 14898.0 44 15139.0 45 15455.0 46 15685.0 47 18456.5 48 24709.0 49 23681.0 50 17409.5 51 14444.0 52 12476.0 53 11353.0 54 10646.0 55 9597.0 56 8319.0 57 7208.5 58 6183.5 59 5208.0 60 4156.5 61 3204.5 62 2583.0 63 1919.0 64 1357.0 65 1043.5 66 748.0 67 597.0 68 401.5 69 215.5 70 152.0 71 116.0 72 92.5 73 73.0 74 49.5 75 36.0 76 19.0 77 7.0 78 6.0 79 3.5 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01558730402454884 2 0.0 3 0.0 4 0.0 5 0.11073965247291415 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004303957081405277 22-23 2.3264632872460958E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0010469084792607431 92-93 0.0 94-95 4.6529265744921916E-4 96-97 0.0 98-99 0.0017448474654345719 100-101 1.1632316436230479E-4 102-103 3.489694930869143E-4 104-105 0.0018611706297968766 106-107 0.006630420368651372 108-109 2.3264632872460958E-4 110-111 0.004303957081405277 112-113 0.0074446825191875066 114-115 0.004303957081405277 116-117 9.305853148984383E-4 118-119 1.1632316436230479E-4 120-121 5.81615821811524E-4 122-123 8.142621505361335E-4 124-125 0.001512201136709962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 429837.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.75051105210639 #Duplication Level Percentage of deduplicated Percentage of total 1 86.97511579200498 63.27484122684493 2 8.974269891752988 13.057654418891229 3 2.036535602782961 4.444770176348099 4 0.7334390093932944 2.1343225103565135 5 0.36092007058099107 1.3128559791864705 6 0.21312150001350544 0.9302818825304411 7 0.13887263734335478 0.7072138736518027 8 0.09832337846919618 0.5722460825602957 9 0.07036009027780982 0.46068592728447055 >10 0.35906047373193933 4.767924702234386 >50 0.020150703112411905 0.9822025446422408 >100 0.014713211688424254 2.211991787554099 >500 0.002878671852083006 1.7305473305322778 >1k 0.00223896699606456 3.4124615573827963 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4347 1.0113135909658777 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2264 0.5267112882325161 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2144 0.4987937287855629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1707 0.3971272831329085 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1481 0.34454921284114676 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1462 0.3401289325953792 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1260 0.2931343741930081 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 973 0.2263648778490451 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 962 0.22380576823307438 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 948 0.22054871963092987 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 901 0.20961434218087321 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 889 0.2068225862361779 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 738 0.17169299059876186 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 702 0.1633177227646759 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 672 0.1563383329029376 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 652 0.15168540632844543 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 449 0.10445820159734968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.652926574492191E-4 0.0 24-25 0.0 0.0 0.0 5.816158218115238E-4 0.0 26-27 0.0 0.0 0.0 0.001163231643623048 0.0 28-29 0.0 0.0 0.0 0.002210140122883791 0.0 30-31 0.0 0.0 0.0 0.0052345423963037156 0.0 32-33 0.0 0.0 0.0 0.012330255422404308 0.0 34-35 0.0 0.0 0.0 0.02361360236554787 0.0 36-37 0.0 0.0 0.0 0.04245795499224125 0.0 38-39 0.0 0.0 0.0 0.07712225797220806 0.0 40-41 0.0 0.0 0.0 0.12330255422404307 0.0 42-43 0.0 0.0 0.0 0.16250346061413978 0.0 44-45 0.0 0.0 0.0 0.20682258623617789 0.0 46-47 0.0 0.0 0.0 0.25835374804867894 0.0 48-49 0.0 0.0 0.0 0.32186619579049736 0.0 50-51 0.0 0.0 0.0 0.3932886187089525 0.0 52-53 0.0 0.0 0.0 0.45819694442311854 0.0 54-55 0.0 0.0 0.0 0.5313642148070082 0.0 56-57 0.0 0.0 0.0 0.6248880389543012 0.0 58-59 0.0 0.0 0.0 0.7314400575101725 0.0 60-61 0.0 0.0 0.0 0.8426450026405359 0.0 62-63 0.0 0.0 0.0 0.9493133443607693 0.0 64-65 0.0 0.0 0.0 1.0545858081086552 0.0 66-67 0.0 0.0 0.0 1.1810290877704803 0.0 68-69 0.0 0.0 0.0 1.329690091825506 0.0 70-71 0.0 0.0 0.0 1.505687039505673 0.0 72-73 0.0 0.0 0.0 1.6821492798432893 0.0 74-75 0.0 0.0 0.0 1.8550055020856744 0.0 76-77 0.0 0.0 0.0 2.035422730011609 0.0 78-79 0.0 0.0 0.0 2.2570183581217997 0.0 80-81 0.0 0.0 0.0 2.504903021377871 0.0 82-83 0.0 0.0 0.0 2.755230471085551 0.0 84-85 0.0 0.0 0.0 2.9974152992878693 0.0 86-87 0.0 0.0 0.0 3.2428571760923326 0.0 88-89 0.0 0.0 0.0 3.521683801068777 0.0 90-91 0.0 0.0 0.0 3.83878074712042 0.0 92-93 0.0 0.0 0.0 4.197986678671217 0.0 94-95 0.0 0.0 0.0 4.5164795026952085 0.0 96-97 0.0 0.0 0.0 4.849512722264486 0.0 98-99 0.0 0.0 0.0 5.205926897870588 0.0 100-101 0.0 0.0 0.0 5.606543875934365 0.0 102-103 0.0 0.0 0.0 6.051014686962732 0.0 104-105 0.0 0.0 0.0 6.4629150119696535 0.0 106-107 0.0 0.0 0.0 6.877490769756908 0.0 108-109 0.0 0.0 0.0 7.307537508404348 0.0 110-111 0.0 0.0 0.0 7.781321756852016 0.0 112-113 0.0 0.0 0.0 8.300123069907896 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGAAC 30 0.0042363736 59.49424 6 TCGTTCA 75 0.0039096386 31.730263 9 GTACATG 3045 0.0 30.920937 1 TACATGG 3085 0.0 30.319782 2 ACATGGG 2990 0.0 28.892056 3 GAGTACT 2895 0.0 28.668211 12-13 AGTACTT 2900 0.0 28.31105 12-13 CATGGGG 1425 0.0 27.593748 4 CGGTTAC 65 6.3806285E-5 27.458881 28-29 CGAAACA 55 7.7540183E-4 27.04284 52-53 GTGTAGC 375 0.0 27.014719 1 GTACTTT 3075 0.0 26.798153 14-15 GATCACG 45 0.009532148 26.441885 48-49 TCGAAAC 45 0.009532148 26.441885 52-53 GTCCTAC 430 0.0 24.945192 1 AGAGTAC 4625 0.0 24.762466 10-11 TACCTGG 385 0.0 24.759443 2 TGTAGCC 440 0.0 24.372578 2 TAGCCCT 470 0.0 24.084486 4 TACTTTT 3420 0.0 23.920858 14-15 >>END_MODULE